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Characterization and Comparative Analysis of the Genes Encoding Haemophilus parasuis Outer Membrane Proteins P2 and P5

Haemophilus parasuis is a swine pathogen of significant industry concern, but little is known about how the organism causes disease. A related human pathogen, Haemophilus influenzae, has been better studied, and many of its virulence factors have been identified. Two of these, outer membrane protein... Full description

Journal Title: The Journal of Bacteriology 2009, Vol. 191(19), p.5988
Main Author: Mullins, Michael A.
Other Authors: Register, Karen B. , Bayles, Darrell O. , Loving, Crystal L. , Nicholson, Tracy L. , Brockmeier, Susan L. , Dyer, David W. , Phillips, Gregory J
Format: Electronic Article Electronic Article
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ID: ISSN: 0021-9193 ; PMID: 19633080
Link: http://jb.asm.org/cgi/content/abstract/191/19/5988
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recordid: asm19633080
title: Characterization and Comparative Analysis of the Genes Encoding Haemophilus parasuis Outer Membrane Proteins P2 and P5
format: Article
creator:
  • Mullins, Michael A.
  • Register, Karen B.
  • Bayles, Darrell O.
  • Loving, Crystal L.
  • Nicholson, Tracy L.
  • Brockmeier, Susan L.
  • Dyer, David W.
  • Phillips, Gregory J
subjects:
  • Epitopes
  • Open Reading Frames
  • Outer Membrane Proteins
  • Messenger Rna
  • Alleles
  • Amino Acid Sequences
  • Genes
  • Nucleotide Sequences
  • Haemophilus Parasuis
  • Gene Expression
  • Bacterial Antigens
  • Bacterial Proteins
  • Ompp5 Gene
  • Molecular Sequence Data
  • Ompp2 Gene
ispartof: The Journal of Bacteriology, 2009, Vol. 191(19), p.5988
description: Haemophilus parasuis is a swine pathogen of significant industry concern, but little is known about how the organism causes disease. A related human pathogen, Haemophilus influenzae, has been better studied, and many of its virulence factors have been identified. Two of these, outer membrane proteins P2 and P5, are known to have important virulence properties. The goals of this study were to identify, analyze, and compare the genetic relatedness of orthologous genes encoding P2 and P5 proteins in a diverse group of 35 H. parasuis strains. Genes encoding P2 and P5 proteins were detected in all H. parasuis strains evaluated. The predicted amino acid sequences for both P2 and P5 proteins exhibit considerable heterogeneity, particularly in regions corresponding to predicted extracellular loops. Twenty-five variants of P2 and 17 variants of P5 were identified. The P2 proteins of seven strains were predicted to contain a highly conserved additional extracellular loop compared to the remaining strains and to H. influenzae P2. Antigenic-site predictions coincided with predicted extracellular loop regions of both P2 and P5. Neighbor-joining trees constructed using P2 and P5 sequences predicted divergent evolutionary histories distinct from those predicted by a multilocus sequence typing phylogeny based on partial sequencing of seven housekeeping genes. Real-time reverse transcription-PCR indicated that both genes are expressed in all of the strains. ; Includes references ; p. 5988-6002.
language:
source:
identifier: ISSN: 0021-9193 ; PMID: 19633080
fulltext: fulltext
issn:
  • 0021-9193
  • 00219193
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titleCharacterization and Comparative Analysis of the Genes Encoding Haemophilus parasuis Outer Membrane Proteins P2 and P5
creatorMullins, Michael A. ; Register, Karen B. ; Bayles, Darrell O. ; Loving, Crystal L. ; Nicholson, Tracy L. ; Brockmeier, Susan L. ; Dyer, David W. ; Phillips, Gregory J
ispartofThe Journal of Bacteriology, 2009, Vol. 191(19), p.5988
identifierISSN: 0021-9193 ; PMID: 19633080
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subjectEpitopes ; Open Reading Frames ; Outer Membrane Proteins ; Messenger Rna ; Alleles ; Amino Acid Sequences ; Genes ; Nucleotide Sequences ; Haemophilus Parasuis ; Gene Expression ; Bacterial Antigens ; Bacterial Proteins ; Ompp5 Gene ; Molecular Sequence Data ; Ompp2 Gene;
descriptionHaemophilus parasuis is a swine pathogen of significant industry concern, but little is known about how the organism causes disease. A related human pathogen, Haemophilus influenzae, has been better studied, and many of its virulence factors have been identified. Two of these, outer membrane proteins P2 and P5, are known to have important virulence properties. The goals of this study were to identify, analyze, and compare the genetic relatedness of orthologous genes encoding P2 and P5 proteins in a diverse group of 35 H. parasuis strains. Genes encoding P2 and P5 proteins were detected in all H. parasuis strains evaluated. The predicted amino acid sequences for both P2 and P5 proteins exhibit considerable heterogeneity, particularly in regions corresponding to predicted extracellular loops. Twenty-five variants of P2 and 17 variants of P5 were identified. The P2 proteins of seven strains were predicted to contain a highly conserved additional extracellular loop compared to the remaining strains and to H. influenzae P2. Antigenic-site predictions coincided with predicted extracellular loop regions of both P2 and P5. Neighbor-joining trees constructed using P2 and P5 sequences predicted divergent evolutionary histories distinct from those predicted by a multilocus sequence typing phylogeny based on partial sequencing of seven housekeeping genes. Real-time reverse transcription-PCR indicated that both genes are expressed in all of the strains. ; Includes references ; p. 5988-6002.
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titleCharacterization and Comparative Analysis of the Genes Encoding Haemophilus parasuis Outer Membrane Proteins P2 and P5
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