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Liposomes produced by reverse phase evaporation: in vitro and in vivo efficacy of diminazene aceturate against Trypanosoma evansi

SUMMARY This study aimed to develop and test the in vitro and in vivo effectiveness of diminazene aceturate encapsulated into liposomes (L-DMZ) on Trypanosoma evansi . To validate the in vitro tests with L-DMZ, the efficacy of a commercial formulation of diminazene aceturate (C-DMZ) was also assesse... Full description

Journal Title: Parasitology 2014, Vol.141(6), pp.761-769
Main Author: Oliveira, Camila Belmonte
Other Authors: Rigo, Lucas Almeida , Rosa, Luciana Dalla , Gressler, Lucas Trevisan , Zimmermann, Carine Eloise Prestes , Ourique, Aline Ferreira , Da Silva, Aleksandro Schafer , Miletti, Luiz C , Beck, Ruy Carlos Ruver , Monteiro, Silvia Gonzalez
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ID: ISSN: 0031-1820 ; E-ISSN: 1469-8161 ; DOI: 10.1017/S0031182013002114
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title: Liposomes produced by reverse phase evaporation: in vitro and in vivo efficacy of diminazene aceturate against Trypanosoma evansi
format: Article
creator:
  • Oliveira, Camila Belmonte
  • Rigo, Lucas Almeida
  • Rosa, Luciana Dalla
  • Gressler, Lucas Trevisan
  • Zimmermann, Carine Eloise Prestes
  • Ourique, Aline Ferreira
  • Da Silva, Aleksandro Schafer
  • Miletti, Luiz C
  • Beck, Ruy Carlos Ruver
  • Monteiro, Silvia Gonzalez
subjects:
  • Nanotechnology
  • Diamidines
  • Trypanosomosis
ispartof: Parasitology, 2014, Vol.141(6), pp.761-769
description: SUMMARY This study aimed to develop and test the in vitro and in vivo effectiveness of diminazene aceturate encapsulated into liposomes (L-DMZ) on Trypanosoma evansi . To validate the in vitro tests with L-DMZ, the efficacy of a commercial formulation of diminazene aceturate (C-DMZ) was also assessed. The tests were carried out in culture medium for T. evansi , at concentrations of 0·25, 0·5, 1, 2 and 3  μ g mL −1 of L-DMZ and C-DMZ. A dose-dependent effect was observed for both formulations (L-DMZ and C-DMZ), with the highest dose-dependent mortality of trypomastigotes being observed at 1 and 3 h after the onset of tests with L-DMZ. The results of in vivo tests showed the same effects in the animals treated with L-DMZ and C-DMZ in single doses of 3·5 mg kg −1 and for 5 consecutive days (3·5 mg kg −1  day −1 ). It was possible to conclude that T. evansi showed greater in vitro susceptibility to L-DMZ when compared with C-DMZ. In vivo tests suggest that treatment with the L-DMZ and C-DMZ showed similar efficacy in vivo . The potential of the formulation developed in this study was clearly demonstrated, as it increased the efficacy of the treatment against trypanosomosis, but more studies are needed to increase the effectiveness in vivo .
language:
source:
identifier: ISSN: 0031-1820 ; E-ISSN: 1469-8161 ; DOI: 10.1017/S0031182013002114
fulltext: fulltext
issn:
  • 00311820
  • 0031-1820
  • 14698161
  • 1469-8161
url: Link


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titleLiposomes produced by reverse phase evaporation: in vitro and in vivo efficacy of diminazene aceturate against Trypanosoma evansi
creatorOliveira, Camila Belmonte ; Rigo, Lucas Almeida ; Rosa, Luciana Dalla ; Gressler, Lucas Trevisan ; Zimmermann, Carine Eloise Prestes ; Ourique, Aline Ferreira ; Da Silva, Aleksandro Schafer ; Miletti, Luiz C ; Beck, Ruy Carlos Ruver ; Monteiro, Silvia Gonzalez
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subjectNanotechnology; Diamidines; Trypanosomosis
descriptionSUMMARY This study aimed to develop and test the in vitro and in vivo effectiveness of diminazene aceturate encapsulated into liposomes (L-DMZ) on Trypanosoma evansi . To validate the in vitro tests with L-DMZ, the efficacy of a commercial formulation of diminazene aceturate (C-DMZ) was also assessed. The tests were carried out in culture medium for T. evansi , at concentrations of 0·25, 0·5, 1, 2 and 3  μ g mL −1 of L-DMZ and C-DMZ. A dose-dependent effect was observed for both formulations (L-DMZ and C-DMZ), with the highest dose-dependent mortality of trypomastigotes being observed at 1 and 3 h after the onset of tests with L-DMZ. The results of in vivo tests showed the same effects in the animals treated with L-DMZ and C-DMZ in single doses of 3·5 mg kg −1 and for 5 consecutive days (3·5 mg kg −1  day −1 ). It was possible to conclude that T. evansi showed greater in vitro susceptibility to L-DMZ when compared with C-DMZ. In vivo tests suggest that treatment with the L-DMZ and C-DMZ showed similar efficacy in vivo . The potential of the formulation developed in this study was clearly demonstrated, as it increased the efficacy of the treatment against trypanosomosis, but more studies are needed to increase the effectiveness in vivo .
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titleLiposomes produced by reverse phase evaporation: in vitro and in vivo efficacy of diminazene aceturate against Trypanosoma evansi
descriptionSUMMARY This study aimed to develop and test the in vitro and in vivo effectiveness of diminazene aceturate encapsulated into liposomes (L-DMZ) on Trypanosoma evansi . To validate the in vitro tests with L-DMZ, the efficacy of a commercial formulation of diminazene aceturate (C-DMZ) was also assessed. The tests were carried out in culture medium for T. evansi , at concentrations of 0·25, 0·5, 1, 2 and 3  μ g mL −1 of L-DMZ and C-DMZ. A dose-dependent effect was observed for both formulations (L-DMZ and C-DMZ), with the highest dose-dependent mortality of trypomastigotes being observed at 1 and 3 h after the onset of tests with L-DMZ. The results of in vivo tests showed the same effects in the animals treated with L-DMZ and C-DMZ in single doses of 3·5 mg kg −1 and for 5 consecutive days (3·5 mg kg −1  day −1 ). It was possible to conclude that T. evansi showed greater in vitro susceptibility to L-DMZ when compared with C-DMZ. In vivo tests suggest that treatment with the L-DMZ and C-DMZ showed similar efficacy in vivo . The potential of the formulation developed in this study was clearly demonstrated, as it increased the efficacy of the treatment against trypanosomosis, but more studies are needed to increase the effectiveness in vivo .
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titleLiposomes produced by reverse phase evaporation: in vitro and in vivo efficacy of diminazene aceturate against Trypanosoma evansi
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atitleLiposomes produced by reverse phase evaporation: in vitro and in vivo efficacy of diminazene aceturate against Trypanosoma evansi
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abstractSUMMARY This study aimed to develop and test the in vitro and in vivo effectiveness of diminazene aceturate encapsulated into liposomes (L-DMZ) on Trypanosoma evansi . To validate the in vitro tests with L-DMZ, the efficacy of a commercial formulation of diminazene aceturate (C-DMZ) was also assessed. The tests were carried out in culture medium for T. evansi , at concentrations of 0·25, 0·5, 1, 2 and 3  μ g mL −1 of L-DMZ and C-DMZ. A dose-dependent effect was observed for both formulations (L-DMZ and C-DMZ), with the highest dose-dependent mortality of trypomastigotes being observed at 1 and 3 h after the onset of tests with L-DMZ. The results of in vivo tests showed the same effects in the animals treated with L-DMZ and C-DMZ in single doses of 3·5 mg kg −1 and for 5 consecutive days (3·5 mg kg −1  day −1 ). It was possible to conclude that T. evansi showed greater in vitro susceptibility to L-DMZ when compared with C-DMZ. In vivo tests suggest that treatment with the L-DMZ and C-DMZ showed similar efficacy in vivo . The potential of the formulation developed in this study was clearly demonstrated, as it increased the efficacy of the treatment against trypanosomosis, but more studies are needed to increase the effectiveness in vivo .
pubCambridge University Press
doi10.1017/S0031182013002114
pages761-769
date2014-05