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Interaction between C1^- channels and CRAC-related Ca^2+ signaling during T lymphocyte activation and proliferation

Aim: To test the hypothesis that C1^- channel blockers affect T cell proliferation through Ca^2+-release-activated Ca^2+ (CRAC) signaling and examine the effects of the combination of a CRAC channel blocker and a C1^- channel blocker on concanavalin A (ConA; 5 mg/mL)-induced Ca^2+ signaling, gene ex... Full description

Journal Title: Acta pharmacologica Sinica 2006, Vol.27 (4), p.437-446
Main Author: Guan-lei WANG Yan QIAN Qin-ying QIU Xiu-jian LAN Hua HE Yong-yuan GUAN
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1671-4083
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recordid: cdi_chongqing_backfile_21532967
title: Interaction between C1^- channels and CRAC-related Ca^2+ signaling during T lymphocyte activation and proliferation
format: Article
creator:
  • Guan-lei WANG Yan QIAN Qin-ying QIU Xiu-jian LAN Hua HE Yong-yuan GUAN
subjects:
  • T淋巴细胞
  • 氯化物
  • 细胞增生
  • 钙离子通道
ispartof: Acta pharmacologica Sinica, 2006, Vol.27 (4), p.437-446
description: Aim: To test the hypothesis that C1^- channel blockers affect T cell proliferation through Ca^2+-release-activated Ca^2+ (CRAC) signaling and examine the effects of the combination of a CRAC channel blocker and a C1^- channel blocker on concanavalin A (ConA; 5 mg/mL)-induced Ca^2+ signaling, gene expression and cellular proliferation in human peripheral T lymphocytes. Methods: [^3H]Thymidine incorporation, Fura-2 fluorescent probe, RNase protection assay, and reverse transcription-polymerase chain reaction were used. Results: The C1^-channel blocker 4,4^1-diisothiocyanostilbene-2,2^1-disulfonic acid (DIDS) inhibited ConAinduced Ca^2+ influx, interleukin-2 mRNA expression and T lymphocyte proliferation in a concentration-dependent manner, and also enhanced the inhibitory effects of 1- {beta-[3-(4-methoxyphenyl)propoxyl]-4-methoxyphenethyl }- 1H-imidazole (SK&F96365) on the above key events during T cell activation. A combination of DIDS (1 μmol/L) and SK&F96365 ( 1 μmol/L) significantly diminished ConAinduced CIC-3 mRNA expression by 64%, whereas DIDS (1 μmol/L) or SK&F96365 (1 μmol/L) alone decreased ConA-induced CIC-3 mRNA expression by only 16% and 9%, respectively. Conclusion: These results suggest that there is an interaction between CRAC-mediated Ca^2+ signaling and DIDS-sensitive C1^- channels during ConA-induced T cell activation and proliferation. Moreover, the DIDSsensitive C1^- channels may be related to the CIC-3 C1^- channels.
language: eng
source:
identifier: ISSN: 1671-4083
fulltext: no_fulltext
issn:
  • 1671-4083
  • 1745-7254
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titleInteraction between C1^- channels and CRAC-related Ca^2+ signaling during T lymphocyte activation and proliferation
creatorGuan-lei WANG Yan QIAN Qin-ying QIU Xiu-jian LAN Hua HE Yong-yuan GUAN
creatorcontribGuan-lei WANG Yan QIAN Qin-ying QIU Xiu-jian LAN Hua HE Yong-yuan GUAN
descriptionAim: To test the hypothesis that C1^- channel blockers affect T cell proliferation through Ca^2+-release-activated Ca^2+ (CRAC) signaling and examine the effects of the combination of a CRAC channel blocker and a C1^- channel blocker on concanavalin A (ConA; 5 mg/mL)-induced Ca^2+ signaling, gene expression and cellular proliferation in human peripheral T lymphocytes. Methods: [^3H]Thymidine incorporation, Fura-2 fluorescent probe, RNase protection assay, and reverse transcription-polymerase chain reaction were used. Results: The C1^-channel blocker 4,4^1-diisothiocyanostilbene-2,2^1-disulfonic acid (DIDS) inhibited ConAinduced Ca^2+ influx, interleukin-2 mRNA expression and T lymphocyte proliferation in a concentration-dependent manner, and also enhanced the inhibitory effects of 1- {beta-[3-(4-methoxyphenyl)propoxyl]-4-methoxyphenethyl }- 1H-imidazole (SK&F96365) on the above key events during T cell activation. A combination of DIDS (1 μmol/L) and SK&F96365 ( 1 μmol/L) significantly diminished ConAinduced CIC-3 mRNA expression by 64%, whereas DIDS (1 μmol/L) or SK&F96365 (1 μmol/L) alone decreased ConA-induced CIC-3 mRNA expression by only 16% and 9%, respectively. Conclusion: These results suggest that there is an interaction between CRAC-mediated Ca^2+ signaling and DIDS-sensitive C1^- channels during ConA-induced T cell activation and proliferation. Moreover, the DIDSsensitive C1^- channels may be related to the CIC-3 C1^- channels.
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descriptionAim: To test the hypothesis that C1^- channel blockers affect T cell proliferation through Ca^2+-release-activated Ca^2+ (CRAC) signaling and examine the effects of the combination of a CRAC channel blocker and a C1^- channel blocker on concanavalin A (ConA; 5 mg/mL)-induced Ca^2+ signaling, gene expression and cellular proliferation in human peripheral T lymphocytes. Methods: [^3H]Thymidine incorporation, Fura-2 fluorescent probe, RNase protection assay, and reverse transcription-polymerase chain reaction were used. Results: The C1^-channel blocker 4,4^1-diisothiocyanostilbene-2,2^1-disulfonic acid (DIDS) inhibited ConAinduced Ca^2+ influx, interleukin-2 mRNA expression and T lymphocyte proliferation in a concentration-dependent manner, and also enhanced the inhibitory effects of 1- {beta-[3-(4-methoxyphenyl)propoxyl]-4-methoxyphenethyl }- 1H-imidazole (SK&F96365) on the above key events during T cell activation. A combination of DIDS (1 μmol/L) and SK&F96365 ( 1 μmol/L) significantly diminished ConAinduced CIC-3 mRNA expression by 64%, whereas DIDS (1 μmol/L) or SK&F96365 (1 μmol/L) alone decreased ConA-induced CIC-3 mRNA expression by only 16% and 9%, respectively. Conclusion: These results suggest that there is an interaction between CRAC-mediated Ca^2+ signaling and DIDS-sensitive C1^- channels during ConA-induced T cell activation and proliferation. Moreover, the DIDSsensitive C1^- channels may be related to the CIC-3 C1^- channels.
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1氯化物
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abstractAim: To test the hypothesis that C1^- channel blockers affect T cell proliferation through Ca^2+-release-activated Ca^2+ (CRAC) signaling and examine the effects of the combination of a CRAC channel blocker and a C1^- channel blocker on concanavalin A (ConA; 5 mg/mL)-induced Ca^2+ signaling, gene expression and cellular proliferation in human peripheral T lymphocytes. Methods: [^3H]Thymidine incorporation, Fura-2 fluorescent probe, RNase protection assay, and reverse transcription-polymerase chain reaction were used. Results: The C1^-channel blocker 4,4^1-diisothiocyanostilbene-2,2^1-disulfonic acid (DIDS) inhibited ConAinduced Ca^2+ influx, interleukin-2 mRNA expression and T lymphocyte proliferation in a concentration-dependent manner, and also enhanced the inhibitory effects of 1- {beta-[3-(4-methoxyphenyl)propoxyl]-4-methoxyphenethyl }- 1H-imidazole (SK&F96365) on the above key events during T cell activation. A combination of DIDS (1 μmol/L) and SK&F96365 ( 1 μmol/L) significantly diminished ConAinduced CIC-3 mRNA expression by 64%, whereas DIDS (1 μmol/L) or SK&F96365 (1 μmol/L) alone decreased ConA-induced CIC-3 mRNA expression by only 16% and 9%, respectively. Conclusion: These results suggest that there is an interaction between CRAC-mediated Ca^2+ signaling and DIDS-sensitive C1^- channels during ConA-induced T cell activation and proliferation. Moreover, the DIDSsensitive C1^- channels may be related to the CIC-3 C1^- channels.