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Genetic characterisation of the erythrocyte-binding protein (PkβII) of Plasmodium knowlesi isolates from Malaysia

Plasmodium knowlesi contributes to the majority of human malaria incidences in Malaysia. Its uncontrollable passage among the natural monkey hosts can potentially lead to zoonotic outbreaks. The merozoite of this parasite invades host erythrocytes through interaction between its erythrocyte-binding... Full description

Journal Title: Journal of genetics 2019-06-14, Vol.98 (3), p.1-5
Main Author: Fong, Mun Yik
Other Authors: Lau, Yee Ling , Jelip, Jenarun , Ooi, Choo Huck , Cheong, Fei Wen
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: New Delhi: Springer India
ID: ISSN: 0022-1333
Link: https://www.ncbi.nlm.nih.gov/pubmed/31544794
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title: Genetic characterisation of the erythrocyte-binding protein (PkβII) of Plasmodium knowlesi isolates from Malaysia
format: Article
creator:
  • Fong, Mun Yik
  • Lau, Yee Ling
  • Jelip, Jenarun
  • Ooi, Choo Huck
  • Cheong, Fei Wen
subjects:
  • Amino acids
  • Analysis
  • Animal Genetics and Genomics
  • Animals
  • beta protein
  • Binding proteins
  • Biological diversity
  • Biomedical and Life Sciences
  • Borneo
  • Erythrocytes - parasitology
  • Evolutionary Biology
  • general
  • genetic diversity
  • Genetic research
  • Haplotypes
  • Life Sciences
  • Life Sciences, general
  • Macaca
  • Malaria
  • Malaria - parasitology
  • Malaria - transmission
  • Malaria - veterinary
  • Malaysia
  • Microbial Genetics and Genomics
  • Monkey Diseases - parasitology
  • Monkey Diseases - transmission
  • Natural selection
  • Phylogeny
  • Plant Genetics and Genomics
  • Plasmodium knowlesi
  • Plasmodium knowlesi - genetics
  • Plasmodium knowlesi - isolation & purification
  • Polymorphism, Genetic
  • Protein binding
  • Protozoan Proteins - blood
  • Protozoan Proteins - genetics
  • Receptors, Cell Surface
  • Research Note
  • Selection, Genetic
  • Sequence Analysis, DNA
ispartof: Journal of genetics, 2019-06-14, Vol.98 (3), p.1-5
description: Plasmodium knowlesi contributes to the majority of human malaria incidences in Malaysia. Its uncontrollable passage among the natural monkey hosts can potentially lead to zoonotic outbreaks. The merozoite of this parasite invades host erythrocytes through interaction between its erythrocyte-binding proteins (EBPs) and their respective receptor on the erythrocytes. The region II of P. knowlesi EBP, P. knowlesi beta ( Pk β II ) protein is found to be mediating merozoite invasion into monkey erythrocytes by interacting with sialic acid receptors. Hence, the objective of this study was to investigate the genetic diversity, natural selection and haplotype grouping of Pk β II of P. knowlesi isolates in Malaysia. Polymerase chain reaction amplifications of Pk β II were performed on archived blood samples from Malaysia and 64 Pk β II sequences were obtained. Sequence analysis revealed length polymorphism, and its amino acids at critical residues indicate the ability of Pk β II to mediate P. knowlesi invasion into monkey erythrocytes. Low genetic diversity ( π = 0.007 ) was observed in the Pk β II of Malaysia Borneo compared to Peninsular Malaysia ( π = 0.015 ). The Pk β II was found to be under strong purifying selection to retain infectivity in monkeys and it plays a limited role in the zoonotic potential of P. knowlesi . Its haplotypes could be clustered into Peninsular Malaysia and Malaysia Borneo groups, indicating the existence of two distinct P. knowlesi parasites in Malaysia as reported in an earlier study.
language: eng
source:
identifier: ISSN: 0022-1333
fulltext: no_fulltext
issn:
  • 0022-1333
  • 0973-7731
url: Link


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titleGenetic characterisation of the erythrocyte-binding protein (PkβII) of Plasmodium knowlesi isolates from Malaysia
creatorFong, Mun Yik ; Lau, Yee Ling ; Jelip, Jenarun ; Ooi, Choo Huck ; Cheong, Fei Wen
creatorcontribFong, Mun Yik ; Lau, Yee Ling ; Jelip, Jenarun ; Ooi, Choo Huck ; Cheong, Fei Wen
descriptionPlasmodium knowlesi contributes to the majority of human malaria incidences in Malaysia. Its uncontrollable passage among the natural monkey hosts can potentially lead to zoonotic outbreaks. The merozoite of this parasite invades host erythrocytes through interaction between its erythrocyte-binding proteins (EBPs) and their respective receptor on the erythrocytes. The region II of P. knowlesi EBP, P. knowlesi beta ( Pk β II ) protein is found to be mediating merozoite invasion into monkey erythrocytes by interacting with sialic acid receptors. Hence, the objective of this study was to investigate the genetic diversity, natural selection and haplotype grouping of Pk β II of P. knowlesi isolates in Malaysia. Polymerase chain reaction amplifications of Pk β II were performed on archived blood samples from Malaysia and 64 Pk β II sequences were obtained. Sequence analysis revealed length polymorphism, and its amino acids at critical residues indicate the ability of Pk β II to mediate P. knowlesi invasion into monkey erythrocytes. Low genetic diversity ( π = 0.007 ) was observed in the Pk β II of Malaysia Borneo compared to Peninsular Malaysia ( π = 0.015 ). The Pk β II was found to be under strong purifying selection to retain infectivity in monkeys and it plays a limited role in the zoonotic potential of P. knowlesi . Its haplotypes could be clustered into Peninsular Malaysia and Malaysia Borneo groups, indicating the existence of two distinct P. knowlesi parasites in Malaysia as reported in an earlier study.
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subjectAmino acids ; Analysis ; Animal Genetics and Genomics ; Animals ; beta protein ; Binding proteins ; Biological diversity ; Biomedical and Life Sciences ; Borneo ; Erythrocytes - parasitology ; Evolutionary Biology ; general ; genetic diversity ; Genetic research ; Haplotypes ; Life Sciences ; Life Sciences, general ; Macaca ; Malaria ; Malaria - parasitology ; Malaria - transmission ; Malaria - veterinary ; Malaysia ; Microbial Genetics and Genomics ; Monkey Diseases - parasitology ; Monkey Diseases - transmission ; Natural selection ; Phylogeny ; Plant Genetics and Genomics ; Plasmodium knowlesi ; Plasmodium knowlesi - genetics ; Plasmodium knowlesi - isolation & purification ; Polymorphism, Genetic ; Protein binding ; Protozoan Proteins - blood ; Protozoan Proteins - genetics ; Receptors, Cell Surface ; Research Note ; Selection, Genetic ; Sequence Analysis, DNA
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0Genetic characterisation of the erythrocyte-binding protein (PkβII) of Plasmodium knowlesi isolates from Malaysia
1Journal of genetics
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descriptionPlasmodium knowlesi contributes to the majority of human malaria incidences in Malaysia. Its uncontrollable passage among the natural monkey hosts can potentially lead to zoonotic outbreaks. The merozoite of this parasite invades host erythrocytes through interaction between its erythrocyte-binding proteins (EBPs) and their respective receptor on the erythrocytes. The region II of P. knowlesi EBP, P. knowlesi beta ( Pk β II ) protein is found to be mediating merozoite invasion into monkey erythrocytes by interacting with sialic acid receptors. Hence, the objective of this study was to investigate the genetic diversity, natural selection and haplotype grouping of Pk β II of P. knowlesi isolates in Malaysia. Polymerase chain reaction amplifications of Pk β II were performed on archived blood samples from Malaysia and 64 Pk β II sequences were obtained. Sequence analysis revealed length polymorphism, and its amino acids at critical residues indicate the ability of Pk β II to mediate P. knowlesi invasion into monkey erythrocytes. Low genetic diversity ( π = 0.007 ) was observed in the Pk β II of Malaysia Borneo compared to Peninsular Malaysia ( π = 0.015 ). The Pk β II was found to be under strong purifying selection to retain infectivity in monkeys and it plays a limited role in the zoonotic potential of P. knowlesi . Its haplotypes could be clustered into Peninsular Malaysia and Malaysia Borneo groups, indicating the existence of two distinct P. knowlesi parasites in Malaysia as reported in an earlier study.
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0Amino acids
1Analysis
2Animal Genetics and Genomics
3Animals
4beta protein
5Binding proteins
6Biological diversity
7Biomedical and Life Sciences
8Borneo
9Erythrocytes - parasitology
10Evolutionary Biology
11general
12genetic diversity
13Genetic research
14Haplotypes
15Life Sciences
16Life Sciences, general
17Macaca
18Malaria
19Malaria - parasitology
20Malaria - transmission
21Malaria - veterinary
22Malaysia
23Microbial Genetics and Genomics
24Monkey Diseases - parasitology
25Monkey Diseases - transmission
26Natural selection
27Phylogeny
28Plant Genetics and Genomics
29Plasmodium knowlesi
30Plasmodium knowlesi - genetics
31Plasmodium knowlesi - isolation & purification
32Polymorphism, Genetic
33Protein binding
34Protozoan Proteins - blood
35Protozoan Proteins - genetics
36Receptors, Cell Surface
37Research Note
38Selection, Genetic
39Sequence Analysis, DNA
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titleGenetic characterisation of the erythrocyte-binding protein (PkβII) of Plasmodium knowlesi isolates from Malaysia
authorFong, Mun Yik ; Lau, Yee Ling ; Jelip, Jenarun ; Ooi, Choo Huck ; Cheong, Fei Wen
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1Analysis
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5Binding proteins
6Biological diversity
7Biomedical and Life Sciences
8Borneo
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18Malaria
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22Malaysia
23Microbial Genetics and Genomics
24Monkey Diseases - parasitology
25Monkey Diseases - transmission
26Natural selection
27Phylogeny
28Plant Genetics and Genomics
29Plasmodium knowlesi
30Plasmodium knowlesi - genetics
31Plasmodium knowlesi - isolation & purification
32Polymorphism, Genetic
33Protein binding
34Protozoan Proteins - blood
35Protozoan Proteins - genetics
36Receptors, Cell Surface
37Research Note
38Selection, Genetic
39Sequence Analysis, DNA
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abstractPlasmodium knowlesi contributes to the majority of human malaria incidences in Malaysia. Its uncontrollable passage among the natural monkey hosts can potentially lead to zoonotic outbreaks. The merozoite of this parasite invades host erythrocytes through interaction between its erythrocyte-binding proteins (EBPs) and their respective receptor on the erythrocytes. The region II of P. knowlesi EBP, P. knowlesi beta ( Pk β II ) protein is found to be mediating merozoite invasion into monkey erythrocytes by interacting with sialic acid receptors. Hence, the objective of this study was to investigate the genetic diversity, natural selection and haplotype grouping of Pk β II of P. knowlesi isolates in Malaysia. Polymerase chain reaction amplifications of Pk β II were performed on archived blood samples from Malaysia and 64 Pk β II sequences were obtained. Sequence analysis revealed length polymorphism, and its amino acids at critical residues indicate the ability of Pk β II to mediate P. knowlesi invasion into monkey erythrocytes. Low genetic diversity ( π = 0.007 ) was observed in the Pk β II of Malaysia Borneo compared to Peninsular Malaysia ( π = 0.015 ). The Pk β II was found to be under strong purifying selection to retain infectivity in monkeys and it plays a limited role in the zoonotic potential of P. knowlesi . Its haplotypes could be clustered into Peninsular Malaysia and Malaysia Borneo groups, indicating the existence of two distinct P. knowlesi parasites in Malaysia as reported in an earlier study.
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