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Inhibition of TPA-induced tumor promotion in CD-1 mouse epidermis by a polyphenolic fraction from grape seeds

The anti-tumor promoting activity of a polyphenolic fraction from grape seeds (GSP) was examined in CD-1 mouse skin epidermis. Specifically, the ability of this fraction to inhibit 12- O-tetradecanoylphorbol-13-acetate (TPA)-induced tumor promotion and two markers of promotion in mouse skin, ornithi... Full description

Journal Title: Cancer letters 1999, Vol.135 (2), p.151-157
Main Author: Bomser, J.A.
Other Authors: Singletary, K.W. , Wallig, M.A. , Smith, M.A.L.
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Alma/SFX Local Collection
Publisher: Shannon: Elsevier Ireland Ltd
ID: ISSN: 0304-3835
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recordid: cdi_crossref_primary_10_1016_S0304_3835_98_00289_4
title: Inhibition of TPA-induced tumor promotion in CD-1 mouse epidermis by a polyphenolic fraction from grape seeds
format: Article
creator:
  • Bomser, J.A.
  • Singletary, K.W.
  • Wallig, M.A.
  • Smith, M.A.L.
subjects:
  • 9,10-Dimethyl-1,2-benzanthracene
  • Animals
  • Anthocyanins
  • Biological and medical sciences
  • Carcinogenesis, carcinogens and anticarcinogens
  • Carcinogens
  • Drug Screening Assays, Antitumor
  • Eflornithine - pharmacology
  • Enzyme Inhibitors - pharmacology
  • Female
  • Flavonoids
  • Foods and miscellaneous
  • Grape seed
  • Medical sciences
  • Mice
  • Neoplasms, Experimental - chemically induced
  • Neoplasms, Experimental - enzymology
  • Neoplasms, Experimental - prevention & control
  • Ornithine Decarboxylase - metabolism
  • Ornithine Decarboxylase Inhibitors
  • Peroxidase - antagonists & inhibitors
  • Peroxidase - metabolism
  • Phenols - chemistry
  • Phenols - therapeutic use
  • Polymers - chemistry
  • Polymers - therapeutic use
  • Polyphenolic
  • Proanthocyanidin
  • Proanthocyanidins
  • Rosales - chemistry
  • Seeds - chemistry
  • Skin Neoplasms - chemically induced
  • Skin Neoplasms - enzymology
  • Skin Neoplasms - prevention & control
  • Skin tumor
  • Tetradecanoylphorbol Acetate
  • Tumors
ispartof: Cancer letters, 1999, Vol.135 (2), p.151-157
description: The anti-tumor promoting activity of a polyphenolic fraction from grape seeds (GSP) was examined in CD-1 mouse skin epidermis. Specifically, the ability of this fraction to inhibit 12- O-tetradecanoylphorbol-13-acetate (TPA)-induced tumor promotion and two markers of promotion in mouse skin, ornithine decarboxylase (ODC) and myeloperoxidase (MPO) activities, was evaluated. Pretreatment of mouse skin with 5, 10, 20 and 30 mg of GSP resulted in a dose-dependent reduction in TPA-induced epidermal ODC activity of 27, 37, 48 and 70%, respectively, compared to controls. In addition, pretreatment of mouse skin with 1, 5, 10 and 20 mg of GSP resulted in a significant 43, 39, 54 and 73% inhibition of MPO activity, respectively, compared to controls. In 7,12-dimethylbenz[a]anthracene (DMBA)-initiated CD-1 mice, biweekly treatment of mouse skin with 5, 10, and 20 mg of GSP 20 min prior to TPA application resulted in a 30, 40, and 60% inhibition of final skin tumor incidence, respectively, compared to controls. In addition, the final number of tumors per mouse in the 5, 10 and 20 mg GSP-treated animals was decreased 63, 51, and 94%, respectively, compared to controls. These studies indicate that GSP possesses anti-tumor promoting activity when applied to CD-1 mouse skin prior to treatment with TPA. The mechanism of this tumor inhibition is due, in part, to a GSP-associated inhibition of TPA-induced epidermal ODC and MPO activities. Thus, GSP warrants further evaluation as a skin cancer chemopreventative agent.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0304-3835
fulltext: fulltext
issn:
  • 0304-3835
  • 1872-7980
url: Link


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titleInhibition of TPA-induced tumor promotion in CD-1 mouse epidermis by a polyphenolic fraction from grape seeds
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descriptionThe anti-tumor promoting activity of a polyphenolic fraction from grape seeds (GSP) was examined in CD-1 mouse skin epidermis. Specifically, the ability of this fraction to inhibit 12- O-tetradecanoylphorbol-13-acetate (TPA)-induced tumor promotion and two markers of promotion in mouse skin, ornithine decarboxylase (ODC) and myeloperoxidase (MPO) activities, was evaluated. Pretreatment of mouse skin with 5, 10, 20 and 30 mg of GSP resulted in a dose-dependent reduction in TPA-induced epidermal ODC activity of 27, 37, 48 and 70%, respectively, compared to controls. In addition, pretreatment of mouse skin with 1, 5, 10 and 20 mg of GSP resulted in a significant 43, 39, 54 and 73% inhibition of MPO activity, respectively, compared to controls. In 7,12-dimethylbenz[a]anthracene (DMBA)-initiated CD-1 mice, biweekly treatment of mouse skin with 5, 10, and 20 mg of GSP 20 min prior to TPA application resulted in a 30, 40, and 60% inhibition of final skin tumor incidence, respectively, compared to controls. In addition, the final number of tumors per mouse in the 5, 10 and 20 mg GSP-treated animals was decreased 63, 51, and 94%, respectively, compared to controls. These studies indicate that GSP possesses anti-tumor promoting activity when applied to CD-1 mouse skin prior to treatment with TPA. The mechanism of this tumor inhibition is due, in part, to a GSP-associated inhibition of TPA-induced epidermal ODC and MPO activities. Thus, GSP warrants further evaluation as a skin cancer chemopreventative agent.
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languageeng
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subject9,10-Dimethyl-1,2-benzanthracene ; Animals ; Anthocyanins ; Biological and medical sciences ; Carcinogenesis, carcinogens and anticarcinogens ; Carcinogens ; Drug Screening Assays, Antitumor ; Eflornithine - pharmacology ; Enzyme Inhibitors - pharmacology ; Female ; Flavonoids ; Foods and miscellaneous ; Grape seed ; Medical sciences ; Mice ; Neoplasms, Experimental - chemically induced ; Neoplasms, Experimental - enzymology ; Neoplasms, Experimental - prevention & control ; Ornithine Decarboxylase - metabolism ; Ornithine Decarboxylase Inhibitors ; Peroxidase - antagonists & inhibitors ; Peroxidase - metabolism ; Phenols - chemistry ; Phenols - therapeutic use ; Polymers - chemistry ; Polymers - therapeutic use ; Polyphenolic ; Proanthocyanidin ; Proanthocyanidins ; Rosales - chemistry ; Seeds - chemistry ; Skin Neoplasms - chemically induced ; Skin Neoplasms - enzymology ; Skin Neoplasms - prevention & control ; Skin tumor ; Tetradecanoylphorbol Acetate ; Tumors
ispartofCancer letters, 1999, Vol.135 (2), p.151-157
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1Cancer letters
addtitleCancer Lett
descriptionThe anti-tumor promoting activity of a polyphenolic fraction from grape seeds (GSP) was examined in CD-1 mouse skin epidermis. Specifically, the ability of this fraction to inhibit 12- O-tetradecanoylphorbol-13-acetate (TPA)-induced tumor promotion and two markers of promotion in mouse skin, ornithine decarboxylase (ODC) and myeloperoxidase (MPO) activities, was evaluated. Pretreatment of mouse skin with 5, 10, 20 and 30 mg of GSP resulted in a dose-dependent reduction in TPA-induced epidermal ODC activity of 27, 37, 48 and 70%, respectively, compared to controls. In addition, pretreatment of mouse skin with 1, 5, 10 and 20 mg of GSP resulted in a significant 43, 39, 54 and 73% inhibition of MPO activity, respectively, compared to controls. In 7,12-dimethylbenz[a]anthracene (DMBA)-initiated CD-1 mice, biweekly treatment of mouse skin with 5, 10, and 20 mg of GSP 20 min prior to TPA application resulted in a 30, 40, and 60% inhibition of final skin tumor incidence, respectively, compared to controls. In addition, the final number of tumors per mouse in the 5, 10 and 20 mg GSP-treated animals was decreased 63, 51, and 94%, respectively, compared to controls. These studies indicate that GSP possesses anti-tumor promoting activity when applied to CD-1 mouse skin prior to treatment with TPA. The mechanism of this tumor inhibition is due, in part, to a GSP-associated inhibition of TPA-induced epidermal ODC and MPO activities. Thus, GSP warrants further evaluation as a skin cancer chemopreventative agent.
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09,10-Dimethyl-1,2-benzanthracene
1Animals
2Anthocyanins
3Biological and medical sciences
4Carcinogenesis, carcinogens and anticarcinogens
5Carcinogens
6Drug Screening Assays, Antitumor
7Eflornithine - pharmacology
8Enzyme Inhibitors - pharmacology
9Female
10Flavonoids
11Foods and miscellaneous
12Grape seed
13Medical sciences
14Mice
15Neoplasms, Experimental - chemically induced
16Neoplasms, Experimental - enzymology
17Neoplasms, Experimental - prevention & control
18Ornithine Decarboxylase - metabolism
19Ornithine Decarboxylase Inhibitors
20Peroxidase - antagonists & inhibitors
21Peroxidase - metabolism
22Phenols - chemistry
23Phenols - therapeutic use
24Polymers - chemistry
25Polymers - therapeutic use
26Polyphenolic
27Proanthocyanidin
28Proanthocyanidins
29Rosales - chemistry
30Seeds - chemistry
31Skin Neoplasms - chemically induced
32Skin Neoplasms - enzymology
33Skin Neoplasms - prevention & control
34Skin tumor
35Tetradecanoylphorbol Acetate
36Tumors
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titleInhibition of TPA-induced tumor promotion in CD-1 mouse epidermis by a polyphenolic fraction from grape seeds
authorBomser, J.A. ; Singletary, K.W. ; Wallig, M.A. ; Smith, M.A.L.
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09,10-Dimethyl-1,2-benzanthracene
1Animals
2Anthocyanins
3Biological and medical sciences
4Carcinogenesis, carcinogens and anticarcinogens
5Carcinogens
6Drug Screening Assays, Antitumor
7Eflornithine - pharmacology
8Enzyme Inhibitors - pharmacology
9Female
10Flavonoids
11Foods and miscellaneous
12Grape seed
13Medical sciences
14Mice
15Neoplasms, Experimental - chemically induced
16Neoplasms, Experimental - enzymology
17Neoplasms, Experimental - prevention & control
18Ornithine Decarboxylase - metabolism
19Ornithine Decarboxylase Inhibitors
20Peroxidase - antagonists & inhibitors
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22Phenols - chemistry
23Phenols - therapeutic use
24Polymers - chemistry
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26Polyphenolic
27Proanthocyanidin
28Proanthocyanidins
29Rosales - chemistry
30Seeds - chemistry
31Skin Neoplasms - chemically induced
32Skin Neoplasms - enzymology
33Skin Neoplasms - prevention & control
34Skin tumor
35Tetradecanoylphorbol Acetate
36Tumors
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abstractThe anti-tumor promoting activity of a polyphenolic fraction from grape seeds (GSP) was examined in CD-1 mouse skin epidermis. Specifically, the ability of this fraction to inhibit 12- O-tetradecanoylphorbol-13-acetate (TPA)-induced tumor promotion and two markers of promotion in mouse skin, ornithine decarboxylase (ODC) and myeloperoxidase (MPO) activities, was evaluated. Pretreatment of mouse skin with 5, 10, 20 and 30 mg of GSP resulted in a dose-dependent reduction in TPA-induced epidermal ODC activity of 27, 37, 48 and 70%, respectively, compared to controls. In addition, pretreatment of mouse skin with 1, 5, 10 and 20 mg of GSP resulted in a significant 43, 39, 54 and 73% inhibition of MPO activity, respectively, compared to controls. In 7,12-dimethylbenz[a]anthracene (DMBA)-initiated CD-1 mice, biweekly treatment of mouse skin with 5, 10, and 20 mg of GSP 20 min prior to TPA application resulted in a 30, 40, and 60% inhibition of final skin tumor incidence, respectively, compared to controls. In addition, the final number of tumors per mouse in the 5, 10 and 20 mg GSP-treated animals was decreased 63, 51, and 94%, respectively, compared to controls. These studies indicate that GSP possesses anti-tumor promoting activity when applied to CD-1 mouse skin prior to treatment with TPA. The mechanism of this tumor inhibition is due, in part, to a GSP-associated inhibition of TPA-induced epidermal ODC and MPO activities. Thus, GSP warrants further evaluation as a skin cancer chemopreventative agent.
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pmid10096423
doi10.1016/S0304-3835(98)00289-4