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On the role of low-energy electrons in the radiosensitization of DNA by gold nanoparticles

Four different gold nanoparticle (GNP) preparations, including naked GNPs and GNPs coated either with thiolated undecane (S-C(11)H(23)), or with dithiolated diethylenetriaminepentaacetic (DTDTPA) or gadolinium (Gd) DTDTPA chelating agents, were synthesized. The average diameters, for each type of na... Full description

Journal Title: Nanotechnology 2011, Vol.22 (46), p.465101-1-10
Main Author: Xiao, Fangxing
Other Authors: Zheng, Yi , Cloutier, Pierre , He, Yunhui , Hunting, Darel , Sanche, Léon
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Alma/SFX Local Collection
Publisher: England: IOP Publishing
ID: ISSN: 0957-4484
Link: https://www.ncbi.nlm.nih.gov/pubmed/22024607
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recordid: cdi_crossref_primary_10_1088_0957_4484_22_46_465101
title: On the role of low-energy electrons in the radiosensitization of DNA by gold nanoparticles
format: Article
creator:
  • Xiao, Fangxing
  • Zheng, Yi
  • Cloutier, Pierre
  • He, Yunhui
  • Hunting, Darel
  • Sanche, Léon
subjects:
  • Article
  • Chelating Agents - chemistry
  • Chelating Agents - pharmacology
  • DNA - metabolism
  • DNA Breaks - drug effects
  • Electrons
  • Gadolinium - chemistry
  • Gadolinium - pharmacology
  • Gold - chemistry
  • Gold - pharmacology
  • Nanoparticles - chemistry
  • Nanoparticles - ultrastructure
  • Plasmids - drug effects
  • Radiation-Sensitizing Agents - chemistry
  • Radiation-Sensitizing Agents - pharmacology
  • Sulfhydryl Compounds - chemistry
  • Sulfhydryl Compounds - pharmacology
ispartof: Nanotechnology, 2011, Vol.22 (46), p.465101-1-10
description: Four different gold nanoparticle (GNP) preparations, including naked GNPs and GNPs coated either with thiolated undecane (S-C(11)H(23)), or with dithiolated diethylenetriaminepentaacetic (DTDTPA) or gadolinium (Gd) DTDTPA chelating agents, were synthesized. The average diameters, for each type of nanoparticle, are 5 nm, 10 and 13 nm, respectively. Dry films of plasmid DNA pGEM-3Zf(-), DNA with bound GNPs and DNA with coated GNPs were bombarded with 60 keV electrons. The yields of single and double strand breaks were measured as a function of exposure by electrophoresis. The binding of just one GNP without coating to DNA containing 3197 base pairs increases single and double strand breaks by a factor of 2.3 while for GNPs coated with S-C(11)H(23) this factor is reduced to 1.6. The GNPs coated with DTDTPA and DTDTPA:Gd in the same ratio with the DNA, produce essentially no increment in damage. These results could be explained by the attenuation by the coatings of the intensity of the low-energy photoelectrons emitted from the GNPs. Thus, coatings of GNPs may considerably attenuate the short-range low-energy electrons emitted from gold, leading to a considerable decrease of radiosensitization. According to our results, the highest radiosensitization should be obtained with GNPs having the shortest possible ligand, directed to the DNA of cancer cells.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0957-4484
fulltext: fulltext
issn:
  • 0957-4484
  • 1361-6528
url: Link


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descriptionFour different gold nanoparticle (GNP) preparations, including naked GNPs and GNPs coated either with thiolated undecane (S-C(11)H(23)), or with dithiolated diethylenetriaminepentaacetic (DTDTPA) or gadolinium (Gd) DTDTPA chelating agents, were synthesized. The average diameters, for each type of nanoparticle, are 5 nm, 10 and 13 nm, respectively. Dry films of plasmid DNA pGEM-3Zf(-), DNA with bound GNPs and DNA with coated GNPs were bombarded with 60 keV electrons. The yields of single and double strand breaks were measured as a function of exposure by electrophoresis. The binding of just one GNP without coating to DNA containing 3197 base pairs increases single and double strand breaks by a factor of 2.3 while for GNPs coated with S-C(11)H(23) this factor is reduced to 1.6. The GNPs coated with DTDTPA and DTDTPA:Gd in the same ratio with the DNA, produce essentially no increment in damage. These results could be explained by the attenuation by the coatings of the intensity of the low-energy photoelectrons emitted from the GNPs. Thus, coatings of GNPs may considerably attenuate the short-range low-energy electrons emitted from gold, leading to a considerable decrease of radiosensitization. According to our results, the highest radiosensitization should be obtained with GNPs having the shortest possible ligand, directed to the DNA of cancer cells.
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subjectArticle ; Chelating Agents - chemistry ; Chelating Agents - pharmacology ; DNA - metabolism ; DNA Breaks - drug effects ; Electrons ; Gadolinium - chemistry ; Gadolinium - pharmacology ; Gold - chemistry ; Gold - pharmacology ; Nanoparticles - chemistry ; Nanoparticles - ultrastructure ; Plasmids - drug effects ; Radiation-Sensitizing Agents - chemistry ; Radiation-Sensitizing Agents - pharmacology ; Sulfhydryl Compounds - chemistry ; Sulfhydryl Compounds - pharmacology
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abstractFour different gold nanoparticle (GNP) preparations, including naked GNPs and GNPs coated either with thiolated undecane (S-C(11)H(23)), or with dithiolated diethylenetriaminepentaacetic (DTDTPA) or gadolinium (Gd) DTDTPA chelating agents, were synthesized. The average diameters, for each type of nanoparticle, are 5 nm, 10 and 13 nm, respectively. Dry films of plasmid DNA pGEM-3Zf(-), DNA with bound GNPs and DNA with coated GNPs were bombarded with 60 keV electrons. The yields of single and double strand breaks were measured as a function of exposure by electrophoresis. The binding of just one GNP without coating to DNA containing 3197 base pairs increases single and double strand breaks by a factor of 2.3 while for GNPs coated with S-C(11)H(23) this factor is reduced to 1.6. The GNPs coated with DTDTPA and DTDTPA:Gd in the same ratio with the DNA, produce essentially no increment in damage. These results could be explained by the attenuation by the coatings of the intensity of the low-energy photoelectrons emitted from the GNPs. Thus, coatings of GNPs may considerably attenuate the short-range low-energy electrons emitted from gold, leading to a considerable decrease of radiosensitization. According to our results, the highest radiosensitization should be obtained with GNPs having the shortest possible ligand, directed to the DNA of cancer cells.
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