schliessen

Filtern

 

Bibliotheken

Chitosan coating of copper nanoparticles reduces in vitro toxicity and increases inflammation in the lung

Despite their potential for a variety of applications, copper nanoparticles induce very strong inflammatory responses and cellular toxicity following aerosolized delivery. Coating metallic nanoparticles with polysaccharides, such as biocompatible and antimicrobial chitosan, has the potential to redu... Full description

Journal Title: Nanotechnology 2013, Vol.24 (39), p.395101-1-10
Main Author: Worthington, Kristan L S
Other Authors: Adamcakova-Dodd, Andrea , Wongrakpanich, Amaraporn , Mudunkotuwa, Imali A , Mapuskar, Kranti A , Joshi, Vijaya B , Allan Guymon, C , Spitz, Douglas R , Grassian, Vicki H , Thorne, Peter S , Salem, Aliasger K
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Alma/SFX Local Collection
Publisher: Bristol: IOP Publishing
ID: ISSN: 0957-4484
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: cdi_crossref_primary_10_1088_0957_4484_24_39_395101
title: Chitosan coating of copper nanoparticles reduces in vitro toxicity and increases inflammation in the lung
format: Article
creator:
  • Worthington, Kristan L S
  • Adamcakova-Dodd, Andrea
  • Wongrakpanich, Amaraporn
  • Mudunkotuwa, Imali A
  • Mapuskar, Kranti A
  • Joshi, Vijaya B
  • Allan Guymon, C
  • Spitz, Douglas R
  • Grassian, Vicki H
  • Thorne, Peter S
  • Salem, Aliasger K
subjects:
  • Administration, Intranasal
  • Animals
  • Bronchoalveolar Lavage Fluid - chemistry
  • Cell Line
  • Cell Survival - drug effects
  • Chitosan
  • Chitosan - administration & dosage
  • Chitosan - pharmacology
  • Coatings
  • Copper
  • Copper - administration & dosage
  • Copper - toxicity
  • Copper Nanoparticles
  • Cross-disciplinary physics: materials science
  • rheology
  • Cytokines
  • Exact sciences and technology
  • Humans
  • Lung - chemistry
  • Lung Inflammation
  • Male
  • Materials science
  • Metal Nanoparticles - administration & dosage
  • Metal Nanoparticles - chemistry
  • Metal Nanoparticles - toxicity
  • Metal Oxide Nanoparticles
  • Mice
  • Mice, Inbred C57BL
  • Nanocrystalline materials
  • Nanoparticle Toxicity
  • Nanoscale materials and structures: fabrication and characterization
  • Physics
  • Pneumonia - chemically induced
ispartof: Nanotechnology, 2013, Vol.24 (39), p.395101-1-10
description: Despite their potential for a variety of applications, copper nanoparticles induce very strong inflammatory responses and cellular toxicity following aerosolized delivery. Coating metallic nanoparticles with polysaccharides, such as biocompatible and antimicrobial chitosan, has the potential to reduce this toxicity. In this study, copper nanoparticles were coated with chitosan using a newly developed and facile method. The presence of coating was confirmed using x-ray photoelectron spectroscopy, rhodamine tagging of chitosan followed by confocal fluorescence imaging of coated particles and observed increases in particle size and zeta potential. Further physical and chemical characteristics were evaluated using dissolution and x-ray diffraction studies. The chitosan coating was shown to significantly reduce the toxicity of copper nanoparticles after 24 and 52 h and the generation of reactive oxygen species as assayed by DHE oxidation after 24 h in vitro. Conversely, inflammatory response, measured using the number of white blood cells, total protein, and cytokines/chemokines in the bronchoalveolar fluid of mice exposed to chitosan coated versus uncoated copper nanoparticles, was shown to increase, as was the concentration of copper ions. These results suggest that coating metal nanoparticles with mucoadhesive polysaccharides (e.g. chitosan) could increase their potential for use in controlled release of copper ions to cells, but will result in a higher inflammatory response if administered via the lung.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0957-4484
fulltext: fulltext
issn:
  • 0957-4484
  • 1361-6528
url: Link


@attributes
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
RANK2.5587912
LOCALfalse
PrimoNMBib
record
control
sourceidproquest_cross
recordidTN_cdi_crossref_primary_10_1088_0957_4484_24_39_395101
sourceformatXML
sourcesystemPC
sourcerecordid1464577985
originalsourceidFETCH-LOGICAL-1585t-165a1fcf4e9110bdd41e77cc3149d897a3d624ae23a97616ba547b2318792ebd3
addsrcrecordideNqFkV1rFDEYhQdR7Lb6F8rcCN5sN2--AyLIolYoeKPXIZPJ7KbMJmOSKe2_N9tdV-tNIZCQnHNykqdpLgFdAZJyhRQTS0olXWG6IqoOBgheNAsgHJacYfmyWZxEZ815zrcIAUgMr5szTBGSGNNF49dbX2I2obXRFB82bRzqcppcaoMJcTKpeDu63CbXz7bOPrR3vqTYlnjvrS8PrQl93bXJmfx4Poxmt6thMezFZevacQ6bN82rwYzZvT3OF83PL59_rK-XN9-_flt_ulkCk6wsgTMDgx2oUwCo63sKTghrCVDVSyUM6TmmxmFilODAO8Oo6DABKRR2XU8umo-H3Gnudq63LpRkRj0lvzPpQUfj9dOT4Ld6E-80kcAV4zXg-hAQJxeMT-6Jtw-u6NhrzIVmRA68q70tFh1IYAyGTipLsYLae6hR749dUvw1u1z0zmfrxtEEF-esgXLKhFCSVSk_SG2KOSc3nG4FpPfI9Z6m3tPUmGqi9AF5NV7--96T7Q_jKnh3FJhszTgkE6zPf3VCIqYUqboP_zWodB8x1m_y4_M98MHu46Rv45xCpfyc6Td7PtZq
sourcetypeOpen Access Repository
isCDItrue
recordtypearticle
pqid1464577985
display
typearticle
titleChitosan coating of copper nanoparticles reduces in vitro toxicity and increases inflammation in the lung
sourceAlma/SFX Local Collection
creatorWorthington, Kristan L S ; Adamcakova-Dodd, Andrea ; Wongrakpanich, Amaraporn ; Mudunkotuwa, Imali A ; Mapuskar, Kranti A ; Joshi, Vijaya B ; Allan Guymon, C ; Spitz, Douglas R ; Grassian, Vicki H ; Thorne, Peter S ; Salem, Aliasger K
creatorcontribWorthington, Kristan L S ; Adamcakova-Dodd, Andrea ; Wongrakpanich, Amaraporn ; Mudunkotuwa, Imali A ; Mapuskar, Kranti A ; Joshi, Vijaya B ; Allan Guymon, C ; Spitz, Douglas R ; Grassian, Vicki H ; Thorne, Peter S ; Salem, Aliasger K
descriptionDespite their potential for a variety of applications, copper nanoparticles induce very strong inflammatory responses and cellular toxicity following aerosolized delivery. Coating metallic nanoparticles with polysaccharides, such as biocompatible and antimicrobial chitosan, has the potential to reduce this toxicity. In this study, copper nanoparticles were coated with chitosan using a newly developed and facile method. The presence of coating was confirmed using x-ray photoelectron spectroscopy, rhodamine tagging of chitosan followed by confocal fluorescence imaging of coated particles and observed increases in particle size and zeta potential. Further physical and chemical characteristics were evaluated using dissolution and x-ray diffraction studies. The chitosan coating was shown to significantly reduce the toxicity of copper nanoparticles after 24 and 52 h and the generation of reactive oxygen species as assayed by DHE oxidation after 24 h in vitro. Conversely, inflammatory response, measured using the number of white blood cells, total protein, and cytokines/chemokines in the bronchoalveolar fluid of mice exposed to chitosan coated versus uncoated copper nanoparticles, was shown to increase, as was the concentration of copper ions. These results suggest that coating metal nanoparticles with mucoadhesive polysaccharides (e.g. chitosan) could increase their potential for use in controlled release of copper ions to cells, but will result in a higher inflammatory response if administered via the lung.
identifier
0ISSN: 0957-4484
1EISSN: 1361-6528
2DOI: 10.1088/0957-4484/24/39/395101
3PMID: 24008224
4CODEN: NNOTER
languageeng
publisherBristol: IOP Publishing
subjectAdministration, Intranasal ; Animals ; Bronchoalveolar Lavage Fluid - chemistry ; Cell Line ; Cell Survival - drug effects ; Chitosan ; Chitosan - administration & dosage ; Chitosan - pharmacology ; Coatings ; Copper ; Copper - administration & dosage ; Copper - toxicity ; Copper Nanoparticles ; Cross-disciplinary physics: materials science; rheology ; Cytokines ; Exact sciences and technology ; Humans ; Lung - chemistry ; Lung Inflammation ; Male ; Materials science ; Metal Nanoparticles - administration & dosage ; Metal Nanoparticles - chemistry ; Metal Nanoparticles - toxicity ; Metal Oxide Nanoparticles ; Mice ; Mice, Inbred C57BL ; Nanocrystalline materials ; Nanoparticle Toxicity ; Nanoscale materials and structures: fabrication and characterization ; Physics ; Pneumonia - chemically induced
ispartofNanotechnology, 2013, Vol.24 (39), p.395101-1-10
rights
02013 IOP Publishing Ltd
12014 INIST-CNRS
lds50peer_reviewed
oafree_for_read
citedbyFETCH-LOGICAL-1585t-165a1fcf4e9110bdd41e77cc3149d897a3d624ae23a97616ba547b2318792ebd3
citesFETCH-LOGICAL-1585t-165a1fcf4e9110bdd41e77cc3149d897a3d624ae23a97616ba547b2318792ebd3
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
thumbnail$$Usyndetics_thumb_exl
backlink
0$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27805993$$DView record in Pascal Francis
1$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24008224$$D View this record in MEDLINE/PubMed
search
creatorcontrib
0Worthington, Kristan L S
1Adamcakova-Dodd, Andrea
2Wongrakpanich, Amaraporn
3Mudunkotuwa, Imali A
4Mapuskar, Kranti A
5Joshi, Vijaya B
6Allan Guymon, C
7Spitz, Douglas R
8Grassian, Vicki H
9Thorne, Peter S
10Salem, Aliasger K
title
0Chitosan coating of copper nanoparticles reduces in vitro toxicity and increases inflammation in the lung
1Nanotechnology
addtitle
0Nano
1Nanotechnology
descriptionDespite their potential for a variety of applications, copper nanoparticles induce very strong inflammatory responses and cellular toxicity following aerosolized delivery. Coating metallic nanoparticles with polysaccharides, such as biocompatible and antimicrobial chitosan, has the potential to reduce this toxicity. In this study, copper nanoparticles were coated with chitosan using a newly developed and facile method. The presence of coating was confirmed using x-ray photoelectron spectroscopy, rhodamine tagging of chitosan followed by confocal fluorescence imaging of coated particles and observed increases in particle size and zeta potential. Further physical and chemical characteristics were evaluated using dissolution and x-ray diffraction studies. The chitosan coating was shown to significantly reduce the toxicity of copper nanoparticles after 24 and 52 h and the generation of reactive oxygen species as assayed by DHE oxidation after 24 h in vitro. Conversely, inflammatory response, measured using the number of white blood cells, total protein, and cytokines/chemokines in the bronchoalveolar fluid of mice exposed to chitosan coated versus uncoated copper nanoparticles, was shown to increase, as was the concentration of copper ions. These results suggest that coating metal nanoparticles with mucoadhesive polysaccharides (e.g. chitosan) could increase their potential for use in controlled release of copper ions to cells, but will result in a higher inflammatory response if administered via the lung.
subject
0Administration, Intranasal
1Animals
2Bronchoalveolar Lavage Fluid - chemistry
3Cell Line
4Cell Survival - drug effects
5Chitosan
6Chitosan - administration & dosage
7Chitosan - pharmacology
8Coatings
9Copper
10Copper - administration & dosage
11Copper - toxicity
12Copper Nanoparticles
13Cross-disciplinary physics: materials science; rheology
14Cytokines
15Exact sciences and technology
16Humans
17Lung - chemistry
18Lung Inflammation
19Male
20Materials science
21Metal Nanoparticles - administration & dosage
22Metal Nanoparticles - chemistry
23Metal Nanoparticles - toxicity
24Metal Oxide Nanoparticles
25Mice
26Mice, Inbred C57BL
27Nanocrystalline materials
28Nanoparticle Toxicity
29Nanoscale materials and structures: fabrication and characterization
30Physics
31Pneumonia - chemically induced
issn
00957-4484
11361-6528
fulltexttrue
rsrctypearticle
creationdate2013
recordtypearticle
recordideNqFkV1rFDEYhQdR7Lb6F8rcCN5sN2--AyLIolYoeKPXIZPJ7KbMJmOSKe2_N9tdV-tNIZCQnHNykqdpLgFdAZJyhRQTS0olXWG6IqoOBgheNAsgHJacYfmyWZxEZ815zrcIAUgMr5szTBGSGNNF49dbX2I2obXRFB82bRzqcppcaoMJcTKpeDu63CbXz7bOPrR3vqTYlnjvrS8PrQl93bXJmfx4Poxmt6thMezFZevacQ6bN82rwYzZvT3OF83PL59_rK-XN9-_flt_ulkCk6wsgTMDgx2oUwCo63sKTghrCVDVSyUM6TmmxmFilODAO8Oo6DABKRR2XU8umo-H3Gnudq63LpRkRj0lvzPpQUfj9dOT4Ld6E-80kcAV4zXg-hAQJxeMT-6Jtw-u6NhrzIVmRA68q70tFh1IYAyGTipLsYLae6hR749dUvw1u1z0zmfrxtEEF-esgXLKhFCSVSk_SG2KOSc3nG4FpPfI9Z6m3tPUmGqi9AF5NV7--96T7Q_jKnh3FJhszTgkE6zPf3VCIqYUqboP_zWodB8x1m_y4_M98MHu46Rv45xCpfyc6Td7PtZq
startdate20131004
enddate20131004
creator
0Worthington, Kristan L S
1Adamcakova-Dodd, Andrea
2Wongrakpanich, Amaraporn
3Mudunkotuwa, Imali A
4Mapuskar, Kranti A
5Joshi, Vijaya B
6Allan Guymon, C
7Spitz, Douglas R
8Grassian, Vicki H
9Thorne, Peter S
10Salem, Aliasger K
general
0IOP Publishing
1Institute of Physics
scope
0IQODW
1CGR
2CUY
3CVF
4ECM
5EIF
6NPM
7AAYXX
8CITATION
97U7
10C1K
11BOBZL
12CLFQK
135PM
sort
creationdate20131004
titleChitosan coating of copper nanoparticles reduces in vitro toxicity and increases inflammation in the lung
authorWorthington, Kristan L S ; Adamcakova-Dodd, Andrea ; Wongrakpanich, Amaraporn ; Mudunkotuwa, Imali A ; Mapuskar, Kranti A ; Joshi, Vijaya B ; Allan Guymon, C ; Spitz, Douglas R ; Grassian, Vicki H ; Thorne, Peter S ; Salem, Aliasger K
facets
frbrtype5
frbrgroupidcdi_FETCH-LOGICAL-1585t-165a1fcf4e9110bdd41e77cc3149d897a3d624ae23a97616ba547b2318792ebd3
rsrctypearticles
prefilterarticles
languageeng
creationdate2013
topic
0Administration, Intranasal
1Animals
2Bronchoalveolar Lavage Fluid - chemistry
3Cell Line
4Cell Survival - drug effects
5Chitosan
6Chitosan - administration & dosage
7Chitosan - pharmacology
8Coatings
9Copper
10Copper - administration & dosage
11Copper - toxicity
12Copper Nanoparticles
13Cross-disciplinary physics: materials science; rheology
14Cytokines
15Exact sciences and technology
16Humans
17Lung - chemistry
18Lung Inflammation
19Male
20Materials science
21Metal Nanoparticles - administration & dosage
22Metal Nanoparticles - chemistry
23Metal Nanoparticles - toxicity
24Metal Oxide Nanoparticles
25Mice
26Mice, Inbred C57BL
27Nanocrystalline materials
28Nanoparticle Toxicity
29Nanoscale materials and structures: fabrication and characterization
30Physics
31Pneumonia - chemically induced
toplevel
0peer_reviewed
1online_resources
creatorcontrib
0Worthington, Kristan L S
1Adamcakova-Dodd, Andrea
2Wongrakpanich, Amaraporn
3Mudunkotuwa, Imali A
4Mapuskar, Kranti A
5Joshi, Vijaya B
6Allan Guymon, C
7Spitz, Douglas R
8Grassian, Vicki H
9Thorne, Peter S
10Salem, Aliasger K
collection
0Pascal-Francis
1Medline
2MEDLINE
3MEDLINE (Ovid)
4MEDLINE
5MEDLINE
6PubMed
7CrossRef
8Toxicology Abstracts
9Environmental Sciences and Pollution Management
10OpenAIRE (Open Access)
11OpenAIRE
12PubMed Central (Full Participant titles)
jtitleNanotechnology
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
au
0Worthington, Kristan L S
1Adamcakova-Dodd, Andrea
2Wongrakpanich, Amaraporn
3Mudunkotuwa, Imali A
4Mapuskar, Kranti A
5Joshi, Vijaya B
6Allan Guymon, C
7Spitz, Douglas R
8Grassian, Vicki H
9Thorne, Peter S
10Salem, Aliasger K
formatjournal
genrearticle
ristypeJOUR
atitleChitosan coating of copper nanoparticles reduces in vitro toxicity and increases inflammation in the lung
jtitleNanotechnology
stitleNano
addtitleNanotechnology
date2013-10-04
risdate2013
volume24
issue39
spage395101
epage1-10
pages395101-1-10
issn0957-4484
eissn1361-6528
codenNNOTER
abstractDespite their potential for a variety of applications, copper nanoparticles induce very strong inflammatory responses and cellular toxicity following aerosolized delivery. Coating metallic nanoparticles with polysaccharides, such as biocompatible and antimicrobial chitosan, has the potential to reduce this toxicity. In this study, copper nanoparticles were coated with chitosan using a newly developed and facile method. The presence of coating was confirmed using x-ray photoelectron spectroscopy, rhodamine tagging of chitosan followed by confocal fluorescence imaging of coated particles and observed increases in particle size and zeta potential. Further physical and chemical characteristics were evaluated using dissolution and x-ray diffraction studies. The chitosan coating was shown to significantly reduce the toxicity of copper nanoparticles after 24 and 52 h and the generation of reactive oxygen species as assayed by DHE oxidation after 24 h in vitro. Conversely, inflammatory response, measured using the number of white blood cells, total protein, and cytokines/chemokines in the bronchoalveolar fluid of mice exposed to chitosan coated versus uncoated copper nanoparticles, was shown to increase, as was the concentration of copper ions. These results suggest that coating metal nanoparticles with mucoadhesive polysaccharides (e.g. chitosan) could increase their potential for use in controlled release of copper ions to cells, but will result in a higher inflammatory response if administered via the lung.
copBristol
pubIOP Publishing
pmid24008224
doi10.1088/0957-4484/24/39/395101
tpages10
oafree_for_read