schliessen

Filtern

 

Bibliotheken

Molecular Identification of Helicoverpa armigera (Lepidoptera: Noctuidae: Heliothinae) in Argentina and Development of a Novel PCR-RFLP Method for its Rapid Differentiation From H. zea and H. gelotopoeon

Helicoverpa armigera (Hu¨ bner) (Lepidoptera: Noctuidae: Heliothinae) is among the most voracious global pests of agriculture. Adults of this species were identified recently in northern Argentina by dissection of male genitalia. In this work, a rapid and simple molecular tool was designed to distin... Full description

Journal Title: Journal of economic entomology 2015, Vol.108 (6), p.2505-2510
Main Author: Arneodo, Joel D
Other Authors: Balbi, Emilia I , Flores, Fernando M , Sciocco-Cap, Alicia
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: England: Entomological Society of America
ID: ISSN: 0022-0493
Link: https://www.ncbi.nlm.nih.gov/pubmed/26318007
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: cdi_crossref_primary_10_1093_jee_tov254
title: Molecular Identification of Helicoverpa armigera (Lepidoptera: Noctuidae: Heliothinae) in Argentina and Development of a Novel PCR-RFLP Method for its Rapid Differentiation From H. zea and H. gelotopoeon
format: Article
creator:
  • Arneodo, Joel D
  • Balbi, Emilia I
  • Flores, Fernando M
  • Sciocco-Cap, Alicia
subjects:
  • Animals
  • Argentina
  • barcode
  • biological invasion
  • Bollworms
  • COI protein
  • Electron Transport Complex IV - genetics
  • Endonuclease
  • Genitalia
  • Helicoverpa
  • Helicoverpa armigera
  • Helicoverpa gelotopoeon
  • Helicoverpa zea
  • Heliothinae
  • Lepidoptera
  • Male
  • Moths - classification
  • Moths - genetics
  • Noctuidae
  • old world bollworm
  • Pests
  • Phylogeny
  • Phylogeography
  • Polymerase chain reaction
  • Polymerase Chain Reaction - methods
  • Polymorphism, Restriction Fragment Length
  • RAPID COMMUNICATION
  • Restriction fragment length polymorphism
  • South America
  • Soybeans
ispartof: Journal of economic entomology, 2015, Vol.108 (6), p.2505-2510
description: Helicoverpa armigera (Hu¨ bner) (Lepidoptera: Noctuidae: Heliothinae) is among the most voracious global pests of agriculture. Adults of this species were identified recently in northern Argentina by dissection of male genitalia. In this work, a rapid and simple molecular tool was designed to distinguish H. armigera from the morphologically similar indigenous bollworms Helicoverpa zea (Boddie) and Helicoverpa gelotopoeon (Dyar), regardless of the life stage. Amplification of partial COI gene with a new primer pair, and subsequent digestion with endonuclease HinfI, yielded different RFLP profiles for the three main Helicoverpa pests currently present in South America. The method was validated in Helicoverpa specimens collected across Argentina, whose identity was further corroborated by COI sequencing and phylogenetic analysis. The data reported here constitute the first molecular confirmation of this pest in the country. The survey revealed the occurrence of H. armigera in northern and central Argentina, including the main soybean- and maize-producing area.
language: eng
source:
identifier: ISSN: 0022-0493
fulltext: no_fulltext
issn:
  • 0022-0493
  • 1938-291X
url: Link


@attributes
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
RANK2.4683754
LOCALfalse
PrimoNMBib
record
control
sourceidproquest_cross
recordidTN_cdi_crossref_primary_10_1093_jee_tov254
sourceformatXML
sourcesystemPC
oup_id10.1093/jee/tov254
sourcerecordid2246906882
originalsourceidFETCH-LOGICAL-1385t-97bc74ee1ff830afc91e90f648bd672253d49cff2d8a1186d0bd6e3a2396c72f0
addsrcrecordideNp9kdFq2zAUhsXYWLNuN3uAIRiDbuBWkh3b6l1Jl6aQbiVssDujyEepgq3jynJge8W91JQ67cUueiXp8J3vHPET8p6zU85kerYFOAu4E9PsBZlwmZaJkPzXSzJhTIiEZTI9Im_6fssYzwVnr8mRyFNeMlZMyN8bbEAPjfL0ugYXrLFaBYuOoqELaKzGHfhOUeVbuwGv6MkSOltjF-LjnH5DHQZbKzh_oDHcWafgM7WOXvjNXuhir6vpJeygwa6Npb1axc5YoLezVbKaL2_pDYQ7rKlBT23o6UrFIfTSGgN-bxl3mnts6eKU_oFRGq-baA3YIaB7S14Z1fTw7nAek5_zrz9mi2T5_ep6drFMeFpOQyKLtS4yAG5MmTJltOQgmcmzcl3nhRDTtM6kNkbUpeK8zGsW65AqkcpcF8KwY3IyejuP9wP0oWptr6FplAMc-ooXmZSSS55F9ON_6BYH7-J2lRBZLlleliJSX0ZKe-x7D6bqvG2V_11xVu0jrmLE1RhxhD8clMO6hfoJfcw0Ap9GAIfuedHhG2uL6OA59B_ImL6e
sourcetypeAggregation Database
isCDItrue
recordtypearticle
pqid2246906882
display
typearticle
titleMolecular Identification of Helicoverpa armigera (Lepidoptera: Noctuidae: Heliothinae) in Argentina and Development of a Novel PCR-RFLP Method for its Rapid Differentiation From H. zea and H. gelotopoeon
creatorArneodo, Joel D ; Balbi, Emilia I ; Flores, Fernando M ; Sciocco-Cap, Alicia
creatorcontribArneodo, Joel D ; Balbi, Emilia I ; Flores, Fernando M ; Sciocco-Cap, Alicia
descriptionHelicoverpa armigera (Hu¨ bner) (Lepidoptera: Noctuidae: Heliothinae) is among the most voracious global pests of agriculture. Adults of this species were identified recently in northern Argentina by dissection of male genitalia. In this work, a rapid and simple molecular tool was designed to distinguish H. armigera from the morphologically similar indigenous bollworms Helicoverpa zea (Boddie) and Helicoverpa gelotopoeon (Dyar), regardless of the life stage. Amplification of partial COI gene with a new primer pair, and subsequent digestion with endonuclease HinfI, yielded different RFLP profiles for the three main Helicoverpa pests currently present in South America. The method was validated in Helicoverpa specimens collected across Argentina, whose identity was further corroborated by COI sequencing and phylogenetic analysis. The data reported here constitute the first molecular confirmation of this pest in the country. The survey revealed the occurrence of H. armigera in northern and central Argentina, including the main soybean- and maize-producing area.
identifier
0ISSN: 0022-0493
1EISSN: 1938-291X
2DOI: 10.1093/jee/tov254
3PMID: 26318007
languageeng
publisherEngland: Entomological Society of America
subjectAnimals ; Argentina ; barcode ; biological invasion ; Bollworms ; COI protein ; Electron Transport Complex IV - genetics ; Endonuclease ; Genitalia ; Helicoverpa ; Helicoverpa armigera ; Helicoverpa gelotopoeon ; Helicoverpa zea ; Heliothinae ; Lepidoptera ; Male ; Moths - classification ; Moths - genetics ; Noctuidae ; old world bollworm ; Pests ; Phylogeny ; Phylogeography ; Polymerase chain reaction ; Polymerase Chain Reaction - methods ; Polymorphism, Restriction Fragment Length ; RAPID COMMUNICATION ; Restriction fragment length polymorphism ; South America ; Soybeans
ispartofJournal of economic entomology, 2015, Vol.108 (6), p.2505-2510
rights
0The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com journals.permissions@oup.com
1The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com 2015
2The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.
3The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com
lds50peer_reviewed
citesFETCH-LOGICAL-1385t-97bc74ee1ff830afc91e90f648bd672253d49cff2d8a1186d0bd6e3a2396c72f0
links
openurl$$Topenurl_article
thumbnail$$Usyndetics_thumb_exl
backlink$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26318007$$D View this record in MEDLINE/PubMed
search
creatorcontrib
0Arneodo, Joel D
1Balbi, Emilia I
2Flores, Fernando M
3Sciocco-Cap, Alicia
title
0Molecular Identification of Helicoverpa armigera (Lepidoptera: Noctuidae: Heliothinae) in Argentina and Development of a Novel PCR-RFLP Method for its Rapid Differentiation From H. zea and H. gelotopoeon
1Journal of economic entomology
addtitleJ Econ Entomol
descriptionHelicoverpa armigera (Hu¨ bner) (Lepidoptera: Noctuidae: Heliothinae) is among the most voracious global pests of agriculture. Adults of this species were identified recently in northern Argentina by dissection of male genitalia. In this work, a rapid and simple molecular tool was designed to distinguish H. armigera from the morphologically similar indigenous bollworms Helicoverpa zea (Boddie) and Helicoverpa gelotopoeon (Dyar), regardless of the life stage. Amplification of partial COI gene with a new primer pair, and subsequent digestion with endonuclease HinfI, yielded different RFLP profiles for the three main Helicoverpa pests currently present in South America. The method was validated in Helicoverpa specimens collected across Argentina, whose identity was further corroborated by COI sequencing and phylogenetic analysis. The data reported here constitute the first molecular confirmation of this pest in the country. The survey revealed the occurrence of H. armigera in northern and central Argentina, including the main soybean- and maize-producing area.
subject
0Animals
1Argentina
2barcode
3biological invasion
4Bollworms
5COI protein
6Electron Transport Complex IV - genetics
7Endonuclease
8Genitalia
9Helicoverpa
10Helicoverpa armigera
11Helicoverpa gelotopoeon
12Helicoverpa zea
13Heliothinae
14Lepidoptera
15Male
16Moths - classification
17Moths - genetics
18Noctuidae
19old world bollworm
20Pests
21Phylogeny
22Phylogeography
23Polymerase chain reaction
24Polymerase Chain Reaction - methods
25Polymorphism, Restriction Fragment Length
26RAPID COMMUNICATION
27Restriction fragment length polymorphism
28South America
29Soybeans
issn
00022-0493
11938-291X
fulltextfalse
rsrctypearticle
creationdate2015
recordtypearticle
recordideNp9kdFq2zAUhsXYWLNuN3uAIRiDbuBWkh3b6l1Jl6aQbiVssDujyEepgq3jynJge8W91JQ67cUueiXp8J3vHPET8p6zU85kerYFOAu4E9PsBZlwmZaJkPzXSzJhTIiEZTI9Im_6fssYzwVnr8mRyFNeMlZMyN8bbEAPjfL0ugYXrLFaBYuOoqELaKzGHfhOUeVbuwGv6MkSOltjF-LjnH5DHQZbKzh_oDHcWafgM7WOXvjNXuhir6vpJeygwa6Npb1axc5YoLezVbKaL2_pDYQ7rKlBT23o6UrFIfTSGgN-bxl3mnts6eKU_oFRGq-baA3YIaB7S14Z1fTw7nAek5_zrz9mi2T5_ep6drFMeFpOQyKLtS4yAG5MmTJltOQgmcmzcl3nhRDTtM6kNkbUpeK8zGsW65AqkcpcF8KwY3IyejuP9wP0oWptr6FplAMc-ooXmZSSS55F9ON_6BYH7-J2lRBZLlleliJSX0ZKe-x7D6bqvG2V_11xVu0jrmLE1RhxhD8clMO6hfoJfcw0Ap9GAIfuedHhG2uL6OA59B_ImL6e
startdate201512
enddate201512
creator
0Arneodo, Joel D
1Balbi, Emilia I
2Flores, Fernando M
3Sciocco-Cap, Alicia
general
0Entomological Society of America
1Oxford University Press
scope
0CGR
1CUY
2CVF
3ECM
4EIF
5NPM
6AAYXX
7CITATION
83V.
97X7
107XB
1188E
128FE
138FH
148FI
158FJ
168FK
178G5
18ABUWG
19AZQEC
20BBNVY
21BENPR
22BHPHI
23DWQXO
24FYUFA
25GHDGH
26GNUQQ
27GUQSH
28HCIFZ
29K9.
30LK8
31M0S
32M1P
33M2O
34M7P
35MBDVC
36PADUT
37PQEST
38PQQKQ
39PQUKI
40PRINS
41Q9U
427X8
sort
creationdate201512
titleMolecular Identification of Helicoverpa armigera (Lepidoptera: Noctuidae: Heliothinae) in Argentina and Development of a Novel PCR-RFLP Method for its Rapid Differentiation From H. zea and H. gelotopoeon
authorArneodo, Joel D ; Balbi, Emilia I ; Flores, Fernando M ; Sciocco-Cap, Alicia
facets
frbrtype5
frbrgroupidcdi_FETCH-LOGICAL-1385t-97bc74ee1ff830afc91e90f648bd672253d49cff2d8a1186d0bd6e3a2396c72f0
rsrctypearticles
prefilterarticles
languageeng
creationdate2015
topic
0Animals
1Argentina
2barcode
3biological invasion
4Bollworms
5COI protein
6Electron Transport Complex IV - genetics
7Endonuclease
8Genitalia
9Helicoverpa
10Helicoverpa armigera
11Helicoverpa gelotopoeon
12Helicoverpa zea
13Heliothinae
14Lepidoptera
15Male
16Moths - classification
17Moths - genetics
18Noctuidae
19old world bollworm
20Pests
21Phylogeny
22Phylogeography
23Polymerase chain reaction
24Polymerase Chain Reaction - methods
25Polymorphism, Restriction Fragment Length
26RAPID COMMUNICATION
27Restriction fragment length polymorphism
28South America
29Soybeans
toplevelpeer_reviewed
creatorcontrib
0Arneodo, Joel D
1Balbi, Emilia I
2Flores, Fernando M
3Sciocco-Cap, Alicia
collection
0Medline
1MEDLINE
2MEDLINE (Ovid)
3MEDLINE
4MEDLINE
5PubMed
6CrossRef
7ProQuest Central (Corporate)
8Health & Medical Collection
9ProQuest Central (purchase pre-March 2016)
10Medical Database (Alumni Edition)
11ProQuest SciTech Collection
12ProQuest Natural Science Collection
13Hospital Premium Collection
14Hospital Premium Collection (Alumni Edition)
15ProQuest Central (Alumni) (purchase pre-March 2016)
16Research Library (Alumni Edition)
17ProQuest Central (Alumni Edition)
18ProQuest Central Essentials
19Biological Science Collection
20ProQuest Central
21Natural Science Collection
22ProQuest Central Korea
23Health Research Premium Collection
24Health Research Premium Collection (Alumni)
25ProQuest Central Student
26Research Library Prep
27SciTech Premium Collection
28ProQuest Health & Medical Complete (Alumni)
29ProQuest Biological Science Collection
30Health & Medical Collection (Alumni Edition)
31Medical Database
32Research Library
33Biological Science Database
34Research Library (Corporate)
35Research Library China
36ProQuest One Academic Eastern Edition
37ProQuest One Academic
38ProQuest One Academic UKI Edition
39ProQuest Central China
40ProQuest Central Basic
41MEDLINE - Academic
jtitleJournal of economic entomology
delivery
delcategoryRemote Search Resource
fulltextno_fulltext
addata
au
0Arneodo, Joel D
1Balbi, Emilia I
2Flores, Fernando M
3Sciocco-Cap, Alicia
formatjournal
genrearticle
ristypeJOUR
atitleMolecular Identification of Helicoverpa armigera (Lepidoptera: Noctuidae: Heliothinae) in Argentina and Development of a Novel PCR-RFLP Method for its Rapid Differentiation From H. zea and H. gelotopoeon
jtitleJournal of economic entomology
addtitleJ Econ Entomol
date2015-12
risdate2015
volume108
issue6
spage2505
epage2510
pages2505-2510
issn0022-0493
eissn1938-291X
abstractHelicoverpa armigera (Hu¨ bner) (Lepidoptera: Noctuidae: Heliothinae) is among the most voracious global pests of agriculture. Adults of this species were identified recently in northern Argentina by dissection of male genitalia. In this work, a rapid and simple molecular tool was designed to distinguish H. armigera from the morphologically similar indigenous bollworms Helicoverpa zea (Boddie) and Helicoverpa gelotopoeon (Dyar), regardless of the life stage. Amplification of partial COI gene with a new primer pair, and subsequent digestion with endonuclease HinfI, yielded different RFLP profiles for the three main Helicoverpa pests currently present in South America. The method was validated in Helicoverpa specimens collected across Argentina, whose identity was further corroborated by COI sequencing and phylogenetic analysis. The data reported here constitute the first molecular confirmation of this pest in the country. The survey revealed the occurrence of H. armigera in northern and central Argentina, including the main soybean- and maize-producing area.
copEngland
pubEntomological Society of America
pmid26318007
doi10.1093/jee/tov254
tpages6