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Telomerase activity in cell lines of pediatric soft tissue sarcomas

Telomeres and their maintenance by telomerase have been implicated to play an important role in carcinogenesis. As almost all malignant tumors express telomerase (in contrast to normal somatic cells), assessment of its activity has been proposed as a diagnostic and prognostic tool. To test the progn... Full description

Journal Title: Pediatric research 2003, Vol.54 (5), p.718-723
Main Author: KLEIDEITER, Elke
Other Authors: SCHWAB, Matthias , FRIEDRICH, Ulrike , KOSCIELNIAK, Ewa , SCHÄFER, Beat W , KLOTZ, Ulrich
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: Hagerstown, MD: Lippincott Williams & Wilkins
ID: ISSN: 0031-3998
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recordid: cdi_crossref_primary_10_1203_01_PDR_0000086901_29111_2A
title: Telomerase activity in cell lines of pediatric soft tissue sarcomas
format: Article
creator:
  • KLEIDEITER, Elke
  • SCHWAB, Matthias
  • FRIEDRICH, Ulrike
  • KOSCIELNIAK, Ewa
  • SCHÄFER, Beat W
  • KLOTZ, Ulrich
subjects:
  • Adolescent
  • Biological and medical sciences
  • Biomarkers, Tumor
  • Cell Line, Tumor
  • Child
  • DNA-Binding Proteins
  • Female
  • Fundamental and applied biological sciences. Psychology
  • General aspects
  • Genetics of eukaryotes. Biological and molecular evolution
  • Humans
  • Medical sciences
  • Molecular and cellular biology
  • Neoplasm Proteins - metabolism
  • Prognosis
  • Sarcoma - enzymology
  • Sarcoma - pathology
  • Soft Tissue Neoplasms - enzymology
  • Soft Tissue Neoplasms - pathology
  • Telomerase - genetics
  • Telomerase - metabolism
  • Telomere - metabolism
ispartof: Pediatric research, 2003, Vol.54 (5), p.718-723
description: Telomeres and their maintenance by telomerase have been implicated to play an important role in carcinogenesis. As almost all malignant tumors express telomerase (in contrast to normal somatic cells), assessment of its activity has been proposed as a diagnostic and prognostic tool. To test the prognostic value of telomerase in pediatric soft tissue sarcoma (STS), we analyzed telomere length (by telomere restriction fragment analysis), telomerase activity (by modified telomerase repeat amplification protocol assay), and expression of human telomerase reverse transcriptase (hTERT) mRNA (by TaqMan technique) in cell lines of different types of STS from 12 children and adolescents. Telomere length (3.7-9.0 kb) showed a very heterogeneous pattern, independent of subtype of STS or the age of the patients, and it was not associated with expression of hTERT mRNA. In contrast, there was a trend of an association between hTERT and telomerase activity. The three tested cell lines of embryonal rhabdomyosarcomas demonstrated no or low (n = 2) telomerase activity, which was confirmed in two cases by a very low expression of hTERT mRNA. Thus, we suggest that the significant difference (p < 0.01) in the less aggressive clinical behavior of embryonal rhabdomyosarcomas in comparison to other subtypes may be due to differences in telomerase expression. Taken together, our cell line experiments imply that telomerase activity might be a biologic marker for stratification between STS with different clinical prognosis.
language: eng
source:
identifier: ISSN: 0031-3998
fulltext: no_fulltext
issn:
  • 0031-3998
  • 1530-0447
url: Link


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titleTelomerase activity in cell lines of pediatric soft tissue sarcomas
creatorKLEIDEITER, Elke ; SCHWAB, Matthias ; FRIEDRICH, Ulrike ; KOSCIELNIAK, Ewa ; SCHÄFER, Beat W ; KLOTZ, Ulrich
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descriptionTelomeres and their maintenance by telomerase have been implicated to play an important role in carcinogenesis. As almost all malignant tumors express telomerase (in contrast to normal somatic cells), assessment of its activity has been proposed as a diagnostic and prognostic tool. To test the prognostic value of telomerase in pediatric soft tissue sarcoma (STS), we analyzed telomere length (by telomere restriction fragment analysis), telomerase activity (by modified telomerase repeat amplification protocol assay), and expression of human telomerase reverse transcriptase (hTERT) mRNA (by TaqMan technique) in cell lines of different types of STS from 12 children and adolescents. Telomere length (3.7-9.0 kb) showed a very heterogeneous pattern, independent of subtype of STS or the age of the patients, and it was not associated with expression of hTERT mRNA. In contrast, there was a trend of an association between hTERT and telomerase activity. The three tested cell lines of embryonal rhabdomyosarcomas demonstrated no or low (n = 2) telomerase activity, which was confirmed in two cases by a very low expression of hTERT mRNA. Thus, we suggest that the significant difference (p < 0.01) in the less aggressive clinical behavior of embryonal rhabdomyosarcomas in comparison to other subtypes may be due to differences in telomerase expression. Taken together, our cell line experiments imply that telomerase activity might be a biologic marker for stratification between STS with different clinical prognosis.
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subjectAdolescent ; Biological and medical sciences ; Biomarkers, Tumor ; Cell Line, Tumor ; Child ; DNA-Binding Proteins ; Female ; Fundamental and applied biological sciences. Psychology ; General aspects ; Genetics of eukaryotes. Biological and molecular evolution ; Humans ; Medical sciences ; Molecular and cellular biology ; Neoplasm Proteins - metabolism ; Prognosis ; Sarcoma - enzymology ; Sarcoma - pathology ; Soft Tissue Neoplasms - enzymology ; Soft Tissue Neoplasms - pathology ; Telomerase - genetics ; Telomerase - metabolism ; Telomere - metabolism
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descriptionTelomeres and their maintenance by telomerase have been implicated to play an important role in carcinogenesis. As almost all malignant tumors express telomerase (in contrast to normal somatic cells), assessment of its activity has been proposed as a diagnostic and prognostic tool. To test the prognostic value of telomerase in pediatric soft tissue sarcoma (STS), we analyzed telomere length (by telomere restriction fragment analysis), telomerase activity (by modified telomerase repeat amplification protocol assay), and expression of human telomerase reverse transcriptase (hTERT) mRNA (by TaqMan technique) in cell lines of different types of STS from 12 children and adolescents. Telomere length (3.7-9.0 kb) showed a very heterogeneous pattern, independent of subtype of STS or the age of the patients, and it was not associated with expression of hTERT mRNA. In contrast, there was a trend of an association between hTERT and telomerase activity. The three tested cell lines of embryonal rhabdomyosarcomas demonstrated no or low (n = 2) telomerase activity, which was confirmed in two cases by a very low expression of hTERT mRNA. Thus, we suggest that the significant difference (p < 0.01) in the less aggressive clinical behavior of embryonal rhabdomyosarcomas in comparison to other subtypes may be due to differences in telomerase expression. Taken together, our cell line experiments imply that telomerase activity might be a biologic marker for stratification between STS with different clinical prognosis.
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abstractTelomeres and their maintenance by telomerase have been implicated to play an important role in carcinogenesis. As almost all malignant tumors express telomerase (in contrast to normal somatic cells), assessment of its activity has been proposed as a diagnostic and prognostic tool. To test the prognostic value of telomerase in pediatric soft tissue sarcoma (STS), we analyzed telomere length (by telomere restriction fragment analysis), telomerase activity (by modified telomerase repeat amplification protocol assay), and expression of human telomerase reverse transcriptase (hTERT) mRNA (by TaqMan technique) in cell lines of different types of STS from 12 children and adolescents. Telomere length (3.7-9.0 kb) showed a very heterogeneous pattern, independent of subtype of STS or the age of the patients, and it was not associated with expression of hTERT mRNA. In contrast, there was a trend of an association between hTERT and telomerase activity. The three tested cell lines of embryonal rhabdomyosarcomas demonstrated no or low (n = 2) telomerase activity, which was confirmed in two cases by a very low expression of hTERT mRNA. Thus, we suggest that the significant difference (p < 0.01) in the less aggressive clinical behavior of embryonal rhabdomyosarcomas in comparison to other subtypes may be due to differences in telomerase expression. Taken together, our cell line experiments imply that telomerase activity might be a biologic marker for stratification between STS with different clinical prognosis.
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pmid12904596
doi10.1203/01.PDR.0000086901.29111.2A
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