schliessen

Filtern

 

Bibliotheken

Rapid Identification of Mycobacterium Leprae by Polymerase Chain Reaction-restriction Fragment Length Polymorphism Analysis of the Heat Shock Protein 65 Gene from Skin Specimens

INTRODUCTION Leprosy caused by Mvcobacterium leprae (M. leprae), is a chronic granulomatous disease affecting the skin and peripheral nervous system, which is transmitted through direct contact with nontreated or inadequate treatment patients. Diagnosis of leprosy depends on the clinical signs and s... Full description

Journal Title: Chinese Medical Journal 2015, Vol.128 (21), p.2964-2966
Main Author: Zhao, Zheng
Other Authors: Liu, Xi-Wan , Jia, Jun , Cai, Lin , Zhang, Jian-Zhong
Format: Electronic Article Electronic Article
Language: English
Subjects:
DNA
Publisher: China: Medknow Publications Pvt Ltd
ID: ISSN: 0366-6999
Link: https://www.ncbi.nlm.nih.gov/pubmed/26521798
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: cdi_doaj_primary_oai_doaj_org_article_1c0edcc380f147f58bccdc3c44da2dcc
title: Rapid Identification of Mycobacterium Leprae by Polymerase Chain Reaction-restriction Fragment Length Polymorphism Analysis of the Heat Shock Protein 65 Gene from Skin Specimens
format: Article
creator:
  • Zhao, Zheng
  • Liu, Xi-Wan
  • Jia, Jun
  • Cai, Lin
  • Zhang, Jian-Zhong
subjects:
  • Adult
  • Analysis
  • Biopsy
  • Deoxyribonucleic acid
  • Dermatology
  • Diagnosis
  • Disease
  • DNA
  • Enzymes
  • Female
  • Genetic aspects
  • Genetic polymorphisms
  • Heat Shock Protein 65 Gene
  • Heat Shock Protein 65 Gene
  • Mycobacterium Leprae
  • Polymerase Chain Reaction-restriction Fragment Length Polymorphism
  • Heat shock proteins
  • Heat-Shock Proteins - genetics
  • Humans
  • Leprosy
  • Leprosy - diagnosis
  • Leprosy - genetics
  • Leprosy - microbiology
  • Male
  • Medicine
  • Middle Aged
  • Mycobacterium Leprae
  • Mycobacterium leprae - pathogenicity
  • Nervous system
  • Physiological aspects
  • Polymerase Chain Reaction
  • Polymerase Chain Reaction - methods
  • Polymerase Chain Reaction-restriction Fragment Length Polymorphism
  • Polymorphism, Restriction Fragment Length
  • Research
  • restriction Fragment Length Polymorphism
  • Short Communication
  • Skin
  • Skin - metabolism
  • Tuberculosis
  • 快速鉴定
  • 热休克蛋白
  • 结核分枝杆菌
  • 聚合酶链反应
  • 限制性片段长度多态性分析
ispartof: Chinese Medical Journal, 2015, Vol.128 (21), p.2964-2966
description: INTRODUCTION Leprosy caused by Mvcobacterium leprae (M. leprae), is a chronic granulomatous disease affecting the skin and peripheral nervous system, which is transmitted through direct contact with nontreated or inadequate treatment patients. Diagnosis of leprosy depends on the clinical signs and symptoms and slit skin smear positivity. However, it's sometimes similar with other granulomatous disease caused by mycobacterial infection. Early stage leprosy is difficult to diagnose by clinical criterion alone because the sensitivity of acid-fast bacilli staining is quite low. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) shows the great advantage in rapid identification and diagnosis for early cases and has a differentiation between leprosy and nonleprosy cases.
language: eng
source:
identifier: ISSN: 0366-6999
fulltext: no_fulltext
issn:
  • 0366-6999
  • 2542-5641
url: Link


@attributes
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
RANK2.3031366
LOCALfalse
PrimoNMBib
record
control
sourceidwanfang_jour_doaj_
recordidTN_cdi_doaj_primary_oai_doaj_org_article_1c0edcc380f147f58bccdc3c44da2dcc
sourceformatXML
sourcesystemPC
galeidA432973727
cqvip_id666647815
wanfj_idzhcmj201521021
sourcerecordidzhcmj201521021
originalsourceidFETCH-LOGICAL-1714i-2a948431e0f267f7b2d2a3edf35b50b14575aba9b09c5474a3d6f426cce9c6730
addsrcrecordideNpdk01v1DAQhiMEoqVw54QikBCXLf52ckGqKvohFahaOFuOY2-8m9ipnbBa_hX_EO-m3XZJIiWaeefxZOw3y95CcEwgwJ8BZmzGyrI8hqwAnD3LDhElaEYZgc-zw136IHsV4wIARClnL7MDxCiCvCwOs783srd1fllrN1hjlRysd7k3-be18pVUgw527PIr3Qep82qdX_t23ekgo85PG2ldfqOTKhXNgo5DsNvv_CzIeZeQqdDNh2aq8qFvbOzyEyfbdbRxs8zQ6PxCyyG_bbxa5tfBDzpBGc3PtdO5Cb7Lb5cpcttrZRMyvs5eGNlG_eb-fZT9Ovv68_RidvXj_PL05GoGOSR2hmRJCoKhBgYxbniFaiSxrg2mFQUVJJRTWcmyAqWihBOJa2YIYkrpUjGOwVF2OXFrLxeiD7aTYS28tGIb8GEuZBisarWACuhaKVwAAwk3tKiUqhVWhNQSpURifZlY_Vh1SZomE2S7B93PONuIuf8tCKesKEACfJ8AvtdO2qD3G3J6ELWux16sjAAACmRMKRVTFCuKagwoowWtmORFhTUAm44-TsCVdEa6uVj4MaR9ieJPo7oFAjCdEJCeo-zTfevB341pi0Vno9JtK532YxSQoxJTjDlN0g__SXdQWCJaYFgQnlTHk2ou0-isMz79sUp3rTurvNPGpvgJwajkmCP-2Oq2oNGyHZro23Fz0OK-EExCFXyMQZvdkCAQG7-IjSHExhBi8ksqefd0Y3YFDwZ5PAUr3yYvxGU7rnQQSbt0frUHnj0BC1QyIrbeEg_eSqz39_013s3vbJr6w3osXYQXkIJ_WOo0ew
sourcetypeOpen Website
isCDItrue
recordtypearticle
pqid1925831847
display
typearticle
titleRapid Identification of Mycobacterium Leprae by Polymerase Chain Reaction-restriction Fragment Length Polymorphism Analysis of the Heat Shock Protein 65 Gene from Skin Specimens
creatorZhao, Zheng ; Liu, Xi-Wan ; Jia, Jun ; Cai, Lin ; Zhang, Jian-Zhong
creatorcontribZhao, Zheng ; Liu, Xi-Wan ; Jia, Jun ; Cai, Lin ; Zhang, Jian-Zhong
descriptionINTRODUCTION Leprosy caused by Mvcobacterium leprae (M. leprae), is a chronic granulomatous disease affecting the skin and peripheral nervous system, which is transmitted through direct contact with nontreated or inadequate treatment patients. Diagnosis of leprosy depends on the clinical signs and symptoms and slit skin smear positivity. However, it's sometimes similar with other granulomatous disease caused by mycobacterial infection. Early stage leprosy is difficult to diagnose by clinical criterion alone because the sensitivity of acid-fast bacilli staining is quite low. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) shows the great advantage in rapid identification and diagnosis for early cases and has a differentiation between leprosy and nonleprosy cases.
identifier
0ISSN: 0366-6999
1EISSN: 2542-5641
2DOI: 10.4103/0366-6999.168076
3PMID: 26521798
languageeng
publisherChina: Medknow Publications Pvt Ltd
subjectAdult ; Analysis ; Biopsy ; Deoxyribonucleic acid ; Dermatology ; Diagnosis ; Disease ; DNA ; Enzymes ; Female ; Genetic aspects ; Genetic polymorphisms ; Heat Shock Protein 65 Gene ; Heat Shock Protein 65 Gene; Mycobacterium Leprae; Polymerase Chain Reaction-restriction Fragment Length Polymorphism ; Heat shock proteins ; Heat-Shock Proteins - genetics ; Humans ; Leprosy ; Leprosy - diagnosis ; Leprosy - genetics ; Leprosy - microbiology ; Male ; Medicine ; Middle Aged ; Mycobacterium Leprae ; Mycobacterium leprae - pathogenicity ; Nervous system ; Physiological aspects ; Polymerase Chain Reaction ; Polymerase Chain Reaction - methods ; Polymerase Chain Reaction-restriction Fragment Length Polymorphism ; Polymorphism, Restriction Fragment Length ; Research ; restriction Fragment Length Polymorphism ; Short Communication ; Skin ; Skin - metabolism ; Tuberculosis ; 快速鉴定 ; 热休克蛋白 ; 结核分枝杆菌 ; 聚合酶链反应 ; 限制性片段长度多态性分析
ispartofChinese Medical Journal, 2015, Vol.128 (21), p.2964-2966
rights
0COPYRIGHT 2015 Medknow Publications and Media Pvt. Ltd.
1Copyright Medknow Publications & Media Pvt. Ltd. Nov 5, 2015
2Copyright © Wanfang Data Co. Ltd. All Rights Reserved.
3Copyright: © 2015 Chinese Medical Journal 2015
lds50peer_reviewed
oafree_for_read
citesFETCH-LOGICAL-1714i-2a948431e0f267f7b2d2a3edf35b50b14575aba9b09c5474a3d6f426cce9c6730
links
openurl$$Topenurl_article
thumbnail$$Uhttp://image.cqvip.com/vip1000/qk/85656X/85656X.jpg
backlink$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26521798$$D View this record in MEDLINE/PubMed
search
creatorcontrib
0Zhao, Zheng
1Liu, Xi-Wan
2Jia, Jun
3Cai, Lin
4Zhang, Jian-Zhong
title
0Rapid Identification of Mycobacterium Leprae by Polymerase Chain Reaction-restriction Fragment Length Polymorphism Analysis of the Heat Shock Protein 65 Gene from Skin Specimens
1Chinese Medical Journal
addtitleChinese Medical Journal
descriptionINTRODUCTION Leprosy caused by Mvcobacterium leprae (M. leprae), is a chronic granulomatous disease affecting the skin and peripheral nervous system, which is transmitted through direct contact with nontreated or inadequate treatment patients. Diagnosis of leprosy depends on the clinical signs and symptoms and slit skin smear positivity. However, it's sometimes similar with other granulomatous disease caused by mycobacterial infection. Early stage leprosy is difficult to diagnose by clinical criterion alone because the sensitivity of acid-fast bacilli staining is quite low. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) shows the great advantage in rapid identification and diagnosis for early cases and has a differentiation between leprosy and nonleprosy cases.
subject
0Adult
1Analysis
2Biopsy
3Deoxyribonucleic acid
4Dermatology
5Diagnosis
6Disease
7DNA
8Enzymes
9Female
10Genetic aspects
11Genetic polymorphisms
12Heat Shock Protein 65 Gene
13Heat Shock Protein 65 Gene; Mycobacterium Leprae; Polymerase Chain Reaction-restriction Fragment Length Polymorphism
14Heat shock proteins
15Heat-Shock Proteins - genetics
16Humans
17Leprosy
18Leprosy - diagnosis
19Leprosy - genetics
20Leprosy - microbiology
21Male
22Medicine
23Middle Aged
24Mycobacterium Leprae
25Mycobacterium leprae - pathogenicity
26Nervous system
27Physiological aspects
28Polymerase Chain Reaction
29Polymerase Chain Reaction - methods
30Polymerase Chain Reaction-restriction Fragment Length Polymorphism
31Polymorphism, Restriction Fragment Length
32Research
33restriction Fragment Length Polymorphism
34Short Communication
35Skin
36Skin - metabolism
37Tuberculosis
38快速鉴定
39热休克蛋白
40结核分枝杆菌
41聚合酶链反应
42限制性片段长度多态性分析
issn
00366-6999
12542-5641
fulltextfalse
rsrctypearticle
creationdate2015
recordtypearticle
recordideNpdk01v1DAQhiMEoqVw54QikBCXLf52ckGqKvohFahaOFuOY2-8m9ipnbBa_hX_EO-m3XZJIiWaeefxZOw3y95CcEwgwJ8BZmzGyrI8hqwAnD3LDhElaEYZgc-zw136IHsV4wIARClnL7MDxCiCvCwOs783srd1fllrN1hjlRysd7k3-be18pVUgw527PIr3Qep82qdX_t23ekgo85PG2ldfqOTKhXNgo5DsNvv_CzIeZeQqdDNh2aq8qFvbOzyEyfbdbRxs8zQ6PxCyyG_bbxa5tfBDzpBGc3PtdO5Cb7Lb5cpcttrZRMyvs5eGNlG_eb-fZT9Ovv68_RidvXj_PL05GoGOSR2hmRJCoKhBgYxbniFaiSxrg2mFQUVJJRTWcmyAqWihBOJa2YIYkrpUjGOwVF2OXFrLxeiD7aTYS28tGIb8GEuZBisarWACuhaKVwAAwk3tKiUqhVWhNQSpURifZlY_Vh1SZomE2S7B93PONuIuf8tCKesKEACfJ8AvtdO2qD3G3J6ELWux16sjAAACmRMKRVTFCuKagwoowWtmORFhTUAm44-TsCVdEa6uVj4MaR9ieJPo7oFAjCdEJCeo-zTfevB341pi0Vno9JtK532YxSQoxJTjDlN0g__SXdQWCJaYFgQnlTHk2ou0-isMz79sUp3rTurvNPGpvgJwajkmCP-2Oq2oNGyHZro23Fz0OK-EExCFXyMQZvdkCAQG7-IjSHExhBi8ksqefd0Y3YFDwZ5PAUr3yYvxGU7rnQQSbt0frUHnj0BC1QyIrbeEg_eSqz39_013s3vbJr6w3osXYQXkIJ_WOo0ew
startdate2015
enddate2015
creator
0Zhao, Zheng
1Liu, Xi-Wan
2Jia, Jun
3Cai, Lin
4Zhang, Jian-Zhong
general
0Medknow Publications Pvt Ltd
1Medknow Publications and Media Pvt. Ltd
2Lippincott Williams & Wilkins Ovid Technologies
3Department of Dermatology, Peking University People's Hospital, Beijing 100044, China
4Medknow Publications & Media Pvt Ltd
5Wolters Kluwer
scope
02RA
192L
2CQIGP
3W91
4~WA
5CGR
6CUY
7CVF
8ECM
9EIF
10NPM
11AAYXX
12CITATION
133V.
147X7
157XB
1688E
178FI
188FJ
198FK
20ABUWG
21AZQEC
22BENPR
23DWQXO
24FYUFA
25GHDGH
26K9.
27M0S
28M1P
29PIMPY
30PQEST
31PQQKQ
32PQUKI
33PRINS
347X8
352B.
364A8
3792I
3893N
39PSX
40TCJ
41BOBZL
42CLFQK
435PM
44DOAMJ
sort
creationdate2015
titleRapid Identification of Mycobacterium Leprae by Polymerase Chain Reaction-restriction Fragment Length Polymorphism Analysis of the Heat Shock Protein 65 Gene from Skin Specimens
authorZhao, Zheng ; Liu, Xi-Wan ; Jia, Jun ; Cai, Lin ; Zhang, Jian-Zhong
facets
frbrtype5
frbrgroupidcdi_FETCH-LOGICAL-1714i-2a948431e0f267f7b2d2a3edf35b50b14575aba9b09c5474a3d6f426cce9c6730
rsrctypearticles
prefilterarticles
languageeng
creationdate2015
topic
0Adult
1Analysis
2Biopsy
3Deoxyribonucleic acid
4Dermatology
5Diagnosis
6Disease
7DNA
8Enzymes
9Female
10Genetic aspects
11Genetic polymorphisms
12Heat Shock Protein 65 Gene
13Heat Shock Protein 65 Gene; Mycobacterium Leprae; Polymerase Chain Reaction-restriction Fragment Length Polymorphism
14Heat shock proteins
15Heat-Shock Proteins - genetics
16Humans
17Leprosy
18Leprosy - diagnosis
19Leprosy - genetics
20Leprosy - microbiology
21Male
22Medicine
23Middle Aged
24Mycobacterium Leprae
25Mycobacterium leprae - pathogenicity
26Nervous system
27Physiological aspects
28Polymerase Chain Reaction
29Polymerase Chain Reaction - methods
30Polymerase Chain Reaction-restriction Fragment Length Polymorphism
31Polymorphism, Restriction Fragment Length
32Research
33restriction Fragment Length Polymorphism
34Short Communication
35Skin
36Skin - metabolism
37Tuberculosis
38快速鉴定
39热休克蛋白
40结核分枝杆菌
41聚合酶链反应
42限制性片段长度多态性分析
toplevelpeer_reviewed
creatorcontrib
0Zhao, Zheng
1Liu, Xi-Wan
2Jia, Jun
3Cai, Lin
4Zhang, Jian-Zhong
collection
0中文科技期刊数据库
1中文科技期刊数据库-CALIS站点
2中文科技期刊数据库-7.0平台
3中文科技期刊数据库-医药卫生
4中文科技期刊数据库- 镜像站点
5Medline
6MEDLINE
7MEDLINE (Ovid)
8MEDLINE
9MEDLINE
10PubMed
11CrossRef
12ProQuest Central (Corporate)
13Health & Medical Collection
14ProQuest Central (purchase pre-March 2016)
15Medical Database (Alumni Edition)
16Hospital Premium Collection
17Hospital Premium Collection (Alumni Edition)
18ProQuest Central (Alumni) (purchase pre-March 2016)
19ProQuest Central (Alumni Edition)
20ProQuest Central Essentials
21ProQuest Central
22ProQuest Central Korea
23Health Research Premium Collection
24Health Research Premium Collection (Alumni)
25ProQuest Health & Medical Complete (Alumni)
26Health & Medical Collection (Alumni Edition)
27Medical Database
28Publicly Available Content Database
29ProQuest One Academic Eastern Edition
30ProQuest One Academic
31ProQuest One Academic UKI Edition
32ProQuest Central China
33MEDLINE - Academic
34Wanfang Data Journals - Hong Kong
35WANFANG Data Centre
36Wanfang Data Journals
37万方数据期刊 - 香港版
38China Online Journals (COJ)
39China Online Journals (COJ)
40OpenAIRE (Open Access)
41OpenAIRE
42PubMed Central (Full Participant titles)
43DOAJ Directory of Open Access Journals
jtitleChinese Medical Journal
delivery
delcategoryRemote Search Resource
fulltextno_fulltext
addata
au
0Zhao, Zheng
1Liu, Xi-Wan
2Jia, Jun
3Cai, Lin
4Zhang, Jian-Zhong
formatjournal
genrearticle
ristypeJOUR
atitleRapid Identification of Mycobacterium Leprae by Polymerase Chain Reaction-restriction Fragment Length Polymorphism Analysis of the Heat Shock Protein 65 Gene from Skin Specimens
jtitleChinese Medical Journal
addtitleChinese Medical Journal
date2015
risdate2015
volume128
issue21
spage2964
epage2966
pages2964-2966
issn0366-6999
eissn2542-5641
notes
0Heat Shock Protein 65 Gene; Mycobacterium Leprae; Polymerase Chain Reaction-restriction Fragment LengthPolymorphism
111-2154/R
2INTRODUCTION Leprosy caused by Mvcobacterium leprae (M. leprae), is a chronic granulomatous disease affecting the skin and peripheral nervous system, which is transmitted through direct contact with nontreated or inadequate treatment patients. Diagnosis of leprosy depends on the clinical signs and symptoms and slit skin smear positivity. However, it's sometimes similar with other granulomatous disease caused by mycobacterial infection. Early stage leprosy is difficult to diagnose by clinical criterion alone because the sensitivity of acid-fast bacilli staining is quite low. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) shows the great advantage in rapid identification and diagnosis for early cases and has a differentiation between leprosy and nonleprosy cases.
abstractINTRODUCTION Leprosy caused by Mvcobacterium leprae (M. leprae), is a chronic granulomatous disease affecting the skin and peripheral nervous system, which is transmitted through direct contact with nontreated or inadequate treatment patients. Diagnosis of leprosy depends on the clinical signs and symptoms and slit skin smear positivity. However, it's sometimes similar with other granulomatous disease caused by mycobacterial infection. Early stage leprosy is difficult to diagnose by clinical criterion alone because the sensitivity of acid-fast bacilli staining is quite low. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) shows the great advantage in rapid identification and diagnosis for early cases and has a differentiation between leprosy and nonleprosy cases.
copChina
pubMedknow Publications Pvt Ltd
pmid26521798
doi10.4103/0366-6999.168076
oafree_for_read