Advanced Methodologies in High-Throughput Sequencing of Immune Repertoires

Journal Title:	Trends in biotechnology (Regular ed.) 2016, Vol.35 (3), p.203-214
Main Author:	Friedensohn, Simon
Other Authors:	Khan, Tarik A , Reddy, Sai T
Format:	Electronic Article
Language:	English
Subjects:	Animals Antibodies Antibodies - genetics Antibodies - immunology antibody Antigens B cell receptor Bias Cloning Computational Biology - methods Datasets Drug discovery Drug Discovery - methods Error correction & detection High-Throughput Nucleotide Sequencing - methods Humans immunoglobulin Immunology Immunotherapy Immunotherapy - methods Internal Medicine Lymphocytes Lymphocytes - immunology Methods Mice Multiculturalism & pluralism next-generation sequencing Sequence Analysis, DNA - methods Sequence Analysis, RNA - methods Single-Cell Analysis - methods Studies T cell receptor T cell receptors T cells Vaccines - genetics Vaccines - immunology Viral antibodies
Quelle:	Alma/SFX Local Collection
Publisher:	England: Elsevier Ltd
ID:	ISSN: 0167-7799
Link:	https://www.ncbi.nlm.nih.gov/pubmed/28341036
Zum Text:

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title:	Advanced Methodologies in High-Throughput Sequencing of Immune Repertoires
format:	Article
creator:	Friedensohn, Simon Khan, Tarik A Reddy, Sai T
subjects:	Animals Antibodies Antibodies - genetics Antibodies - immunology antibody Antigens B cell receptor Bias Cloning Computational Biology - methods Datasets Drug discovery Drug Discovery - methods Error correction & detection High-Throughput Nucleotide Sequencing - methods Humans immunoglobulin Immunology Immunotherapy Immunotherapy - methods Internal Medicine Lymphocytes Lymphocytes - immunology Methods Mice Multiculturalism & pluralism next-generation sequencing Sequence Analysis, DNA - methods Sequence Analysis, RNA - methods Single-Cell Analysis - methods Studies T cell receptor T cell receptors T cells Vaccines - genetics Vaccines - immunology Viral antibodies
ispartof:	Trends in biotechnology (Regular ed.), 2016, Vol.35 (3), p.203-214
description:	In recent years, major efforts have been made to develop sophisticated experimental and bioinformatic workflows for sequencing adaptive immune repertoires. The immunological insight gained has been applied to fields as varied as lymphocyte biology, immunodiagnostics, vaccines, cancer immunotherapy, and antibody engineering. In this review, we provide a detailed overview of these advanced methodologies, focusing specifically on strategies to reduce sequencing errors and bias and to achieve high-throughput pairing of variable regions (e.g., heavy-light or alpha-beta chains). In addition, we highlight recent technologies for single-cell transcriptome sequencing that can be integrated with immune repertoires. Finally, we provide a perspective on advanced immune repertoire sequencing and its ability to impact basic immunology, biopharmaceutical drug discovery and development, and cancer immunotherapy.
language:	eng
source:	Alma/SFX Local Collection
identifier:	ISSN: 0167-7799
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Advanced Methodologies in High-Throughput Sequencing of Immune Repertoires

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Friedensohn, Simon ; Khan, Tarik A ; Reddy, Sai T

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Friedensohn, Simon ; Khan, Tarik A ; Reddy, Sai T

description

In recent years, major efforts have been made to develop sophisticated experimental and bioinformatic workflows for sequencing adaptive immune repertoires. The immunological insight gained has been applied to fields as varied as lymphocyte biology, immunodiagnostics, vaccines, cancer immunotherapy, and antibody engineering. In this review, we provide a detailed overview of these advanced methodologies, focusing specifically on strategies to reduce sequencing errors and bias and to achieve high-throughput pairing of variable regions (e.g., heavy-light or alpha-beta chains). In addition, we highlight recent technologies for single-cell transcriptome sequencing that can be integrated with immune repertoires. Finally, we provide a perspective on advanced immune repertoire sequencing and its ability to impact basic immunology, biopharmaceutical drug discovery and development, and cancer immunotherapy.

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0	ISSN: 0167-7799
1	EISSN: 1879-3096
2	DOI: 10.1016/j.tibtech.2016.09.010
3	PMID: 28341036

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Animals ; Antibodies ; Antibodies - genetics ; Antibodies - immunology ; antibody ; Antigens ; B cell receptor ; Bias ; Cloning ; Computational Biology - methods ; Datasets ; Drug discovery ; Drug Discovery - methods ; Error correction & detection ; High-Throughput Nucleotide Sequencing - methods ; Humans ; immunoglobulin ; Immunology ; Immunotherapy ; Immunotherapy - methods ; Internal Medicine ; Lymphocytes ; Lymphocytes - immunology ; Methods ; Mice ; Multiculturalism & pluralism ; next-generation sequencing ; Sequence Analysis, DNA - methods ; Sequence Analysis, RNA - methods ; Single-Cell Analysis - methods ; Studies ; T cell receptor ; T cell receptors ; T cells ; Vaccines - genetics ; Vaccines - immunology ; Viral antibodies

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Trends in biotechnology (Regular ed.), 2016, Vol.35 (3), p.203-214

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0	Elsevier Ltd
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3	COPYRIGHT 2017 Elsevier B.V.
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title	Advanced Methodologies in High-Throughput Sequencing of Immune Repertoires
author	Friedensohn, Simon ; Khan, Tarik A ; Reddy, Sai T

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1	Antibodies
2	Antibodies - genetics
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8	Cloning
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30	Studies
31	T cell receptor
32	T cell receptors
33	T cells
34	Vaccines - genetics
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Advanced Methodologies in High-Throughput Sequencing of Immune Repertoires

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England

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Elsevier Ltd

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Advanced Methodologies in High-Throughput Sequencing of Immune Repertoires