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Advanced Methodologies in High-Throughput Sequencing of Immune Repertoires

In recent years, major efforts have been made to develop sophisticated experimental and bioinformatic workflows for sequencing adaptive immune repertoires. The immunological insight gained has been applied to fields as varied as lymphocyte biology, immunodiagnostics, vaccines, cancer immunotherapy,... Full description

Journal Title: Trends in biotechnology (Regular ed.) 2016, Vol.35 (3), p.203-214
Main Author: Friedensohn, Simon
Other Authors: Khan, Tarik A , Reddy, Sai T
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Alma/SFX Local Collection
Publisher: England: Elsevier Ltd
ID: ISSN: 0167-7799
Link: https://www.ncbi.nlm.nih.gov/pubmed/28341036
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title: Advanced Methodologies in High-Throughput Sequencing of Immune Repertoires
format: Article
creator:
  • Friedensohn, Simon
  • Khan, Tarik A
  • Reddy, Sai T
subjects:
  • Animals
  • Antibodies
  • Antibodies - genetics
  • Antibodies - immunology
  • antibody
  • Antigens
  • B cell receptor
  • Bias
  • Cloning
  • Computational Biology - methods
  • Datasets
  • Drug discovery
  • Drug Discovery - methods
  • Error correction & detection
  • High-Throughput Nucleotide Sequencing - methods
  • Humans
  • immunoglobulin
  • Immunology
  • Immunotherapy
  • Immunotherapy - methods
  • Internal Medicine
  • Lymphocytes
  • Lymphocytes - immunology
  • Methods
  • Mice
  • Multiculturalism & pluralism
  • next-generation sequencing
  • Sequence Analysis, DNA - methods
  • Sequence Analysis, RNA - methods
  • Single-Cell Analysis - methods
  • Studies
  • T cell receptor
  • T cell receptors
  • T cells
  • Vaccines - genetics
  • Vaccines - immunology
  • Viral antibodies
ispartof: Trends in biotechnology (Regular ed.), 2016, Vol.35 (3), p.203-214
description: In recent years, major efforts have been made to develop sophisticated experimental and bioinformatic workflows for sequencing adaptive immune repertoires. The immunological insight gained has been applied to fields as varied as lymphocyte biology, immunodiagnostics, vaccines, cancer immunotherapy, and antibody engineering. In this review, we provide a detailed overview of these advanced methodologies, focusing specifically on strategies to reduce sequencing errors and bias and to achieve high-throughput pairing of variable regions (e.g., heavy-light or alpha-beta chains). In addition, we highlight recent technologies for single-cell transcriptome sequencing that can be integrated with immune repertoires. Finally, we provide a perspective on advanced immune repertoire sequencing and its ability to impact basic immunology, biopharmaceutical drug discovery and development, and cancer immunotherapy.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0167-7799
fulltext: fulltext
issn:
  • 0167-7799
  • 1879-3096
url: Link


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descriptionIn recent years, major efforts have been made to develop sophisticated experimental and bioinformatic workflows for sequencing adaptive immune repertoires. The immunological insight gained has been applied to fields as varied as lymphocyte biology, immunodiagnostics, vaccines, cancer immunotherapy, and antibody engineering. In this review, we provide a detailed overview of these advanced methodologies, focusing specifically on strategies to reduce sequencing errors and bias and to achieve high-throughput pairing of variable regions (e.g., heavy-light or alpha-beta chains). In addition, we highlight recent technologies for single-cell transcriptome sequencing that can be integrated with immune repertoires. Finally, we provide a perspective on advanced immune repertoire sequencing and its ability to impact basic immunology, biopharmaceutical drug discovery and development, and cancer immunotherapy.
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subjectAnimals ; Antibodies ; Antibodies - genetics ; Antibodies - immunology ; antibody ; Antigens ; B cell receptor ; Bias ; Cloning ; Computational Biology - methods ; Datasets ; Drug discovery ; Drug Discovery - methods ; Error correction & detection ; High-Throughput Nucleotide Sequencing - methods ; Humans ; immunoglobulin ; Immunology ; Immunotherapy ; Immunotherapy - methods ; Internal Medicine ; Lymphocytes ; Lymphocytes - immunology ; Methods ; Mice ; Multiculturalism & pluralism ; next-generation sequencing ; Sequence Analysis, DNA - methods ; Sequence Analysis, RNA - methods ; Single-Cell Analysis - methods ; Studies ; T cell receptor ; T cell receptors ; T cells ; Vaccines - genetics ; Vaccines - immunology ; Viral antibodies
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abstractIn recent years, major efforts have been made to develop sophisticated experimental and bioinformatic workflows for sequencing adaptive immune repertoires. The immunological insight gained has been applied to fields as varied as lymphocyte biology, immunodiagnostics, vaccines, cancer immunotherapy, and antibody engineering. In this review, we provide a detailed overview of these advanced methodologies, focusing specifically on strategies to reduce sequencing errors and bias and to achieve high-throughput pairing of variable regions (e.g., heavy-light or alpha-beta chains). In addition, we highlight recent technologies for single-cell transcriptome sequencing that can be integrated with immune repertoires. Finally, we provide a perspective on advanced immune repertoire sequencing and its ability to impact basic immunology, biopharmaceutical drug discovery and development, and cancer immunotherapy.
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pmid28341036
doi10.1016/j.tibtech.2016.09.010