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Cryopreservation Versus Fresh Frozen Meniscal Allograft Tissue: A Biomechanical Comparative Analysis

Abstract Introduction Meniscal allograft transplantation may offer a better alternative for irreparable meniscal injury. However, it remains to be seen whether a fresh frozen graft is better than a cryopreserved allograft. It is our hypothesis that cryopreserved meniscal allograft will maintain the... Full description

Journal Title: The knee 2015
Main Author: Ahmad, Shukur
Other Authors: Hussein, Shamsul Iskandar , Singh, Vivek Ajit
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Alma/SFX Local Collection
Publisher: Elsevier B.V
ID: ISSN: 0968-0160
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title: Cryopreservation Versus Fresh Frozen Meniscal Allograft Tissue: A Biomechanical Comparative Analysis
format: Article
creator:
  • Ahmad, Shukur
  • Hussein, Shamsul Iskandar
  • Singh, Vivek Ajit
subjects:
  • Orthopedics
ispartof: The knee, 2015
description: Abstract Introduction Meniscal allograft transplantation may offer a better alternative for irreparable meniscal injury. However, it remains to be seen whether a fresh frozen graft is better than a cryopreserved allograft. It is our hypothesis that cryopreserved meniscal allograft will maintain the original biomechanical properties better, due to the lower amount of damage caused during storage process. We examined young and healthy human menisci from orthopedic oncology patients that underwent resections, following either cryopreserved or deep-freezing process. Traction tests were carried out on the menisci after 6 weeks in cold storage. Results Twelve pairs (N=24) of menisci were equally divided into 2 groups, one group were preserved with cryopreservation and the other with deep-freezing. Donors were from male (n=6) and female (n=6) patients undergoing proximal tibia resection, aged between 8 to 35 years old (20.9±8.7 years). As compared to fresh frozen, cryopreserved specimens had higher Ultimate tensile strength (UTS) (8.2±1.3Mpa vs. 13.3±1.7Mpa: p
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0968-0160
fulltext: fulltext
issn:
  • 0968-0160
  • 1873-5800
url: Link


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titleCryopreservation Versus Fresh Frozen Meniscal Allograft Tissue: A Biomechanical Comparative Analysis
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creatorAhmad, Shukur ; Hussein, Shamsul Iskandar ; Singh, Vivek Ajit
creatorcontribAhmad, Shukur ; Hussein, Shamsul Iskandar ; Singh, Vivek Ajit
descriptionAbstract Introduction Meniscal allograft transplantation may offer a better alternative for irreparable meniscal injury. However, it remains to be seen whether a fresh frozen graft is better than a cryopreserved allograft. It is our hypothesis that cryopreserved meniscal allograft will maintain the original biomechanical properties better, due to the lower amount of damage caused during storage process. We examined young and healthy human menisci from orthopedic oncology patients that underwent resections, following either cryopreserved or deep-freezing process. Traction tests were carried out on the menisci after 6 weeks in cold storage. Results Twelve pairs (N=24) of menisci were equally divided into 2 groups, one group were preserved with cryopreservation and the other with deep-freezing. Donors were from male (n=6) and female (n=6) patients undergoing proximal tibia resection, aged between 8 to 35 years old (20.9±8.7 years). As compared to fresh frozen, cryopreserved specimens had higher Ultimate tensile strength (UTS) (8.2±1.3Mpa vs. 13.3±1.7Mpa: p<0.05) and elastic Modulus (61.7±27.6 Mpa Vs. 87.0±44.10 Mpa: p<0.05). There was a significant difference in ultimate tensile strength (p<0.05) between the 2 groups but no significant difference in the elastic modulus (p>0.05). There is no significant difference between the elastic modulus for fresh normal menisci taken from other studies (60 to 120 Mpa) and our samples, cryopreserved and fresh frozen respectively (87.0±44.1 vs 61.7±27.5; p>0.05). Conclusion Higher elastic modulus and point of rupture (ultimate tensile strength) was found in cryopreserved menisci. There was significant comparison between the two preservations in this study that was in favor for cryopreservation.
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abstractAbstract Introduction Meniscal allograft transplantation may offer a better alternative for irreparable meniscal injury. However, it remains to be seen whether a fresh frozen graft is better than a cryopreserved allograft. It is our hypothesis that cryopreserved meniscal allograft will maintain the original biomechanical properties better, due to the lower amount of damage caused during storage process. We examined young and healthy human menisci from orthopedic oncology patients that underwent resections, following either cryopreserved or deep-freezing process. Traction tests were carried out on the menisci after 6 weeks in cold storage. Results Twelve pairs (N=24) of menisci were equally divided into 2 groups, one group were preserved with cryopreservation and the other with deep-freezing. Donors were from male (n=6) and female (n=6) patients undergoing proximal tibia resection, aged between 8 to 35 years old (20.9±8.7 years). As compared to fresh frozen, cryopreserved specimens had higher Ultimate tensile strength (UTS) (8.2±1.3Mpa vs. 13.3±1.7Mpa: p<0.05) and elastic Modulus (61.7±27.6 Mpa Vs. 87.0±44.10 Mpa: p<0.05). There was a significant difference in ultimate tensile strength (p<0.05) between the 2 groups but no significant difference in the elastic modulus (p>0.05). There is no significant difference between the elastic modulus for fresh normal menisci taken from other studies (60 to 120 Mpa) and our samples, cryopreserved and fresh frozen respectively (87.0±44.1 vs 61.7±27.5; p>0.05). Conclusion Higher elastic modulus and point of rupture (ultimate tensile strength) was found in cryopreserved menisci. There was significant comparison between the two preservations in this study that was in favor for cryopreservation.
pubElsevier B.V
doi10.1016/j.knee.2015.09.008