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Subtype-specific neuronal differentiation of PC12 cells transfected with α 2-adrenergic receptors

Cells of the PC12 rat pheochromocytoma cell line acquire characteristics of sympathetic neurons under appropriate treatment. Stably transfected PC12 cells expressing individual α 2-adrenergic receptor (α 2-AR) subtypes were used to assess the role of α 2-ARs in neuronal differentiation and to charac... Full description

Journal Title: European journal of cell biology 2002, Vol.81 (6), p.363-374
Main Author: Taraviras, Stavros
Other Authors: Olli-Lähdesmäki, Tuire , Lymperopoulos, Anastasios , Charitonidou, Despina , Mavroidis, Manolis , Kallio, Jaana , Scheinin, Mika , Flordellis, Christodoulos
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Alma/SFX Local Collection
Publisher: Elsevier GmbH
ID: ISSN: 0171-9335
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recordid: cdi_elsevier_sciencedirect_doi_10_1078_0171_9335_00250
title: Subtype-specific neuronal differentiation of PC12 cells transfected with α 2-adrenergic receptors
format: Article
creator:
  • Taraviras, Stavros
  • Olli-Lähdesmäki, Tuire
  • Lymperopoulos, Anastasios
  • Charitonidou, Despina
  • Mavroidis, Manolis
  • Kallio, Jaana
  • Scheinin, Mika
  • Flordellis, Christodoulos
subjects:
  • AP-1
  • MAP-kinase
  • PI3-kinase
  • signalling
  • α 2-Adrenoceptors
ispartof: European journal of cell biology, 2002, Vol.81 (6), p.363-374
description: Cells of the PC12 rat pheochromocytoma cell line acquire characteristics of sympathetic neurons under appropriate treatment. Stably transfected PC12 cells expressing individual α 2-adrenergic receptor (α 2-AR) subtypes were used to assess the role of α 2-ARs in neuronal differentiation and to characterise the signalling pathways activated by the α 2-AR agonist epinephrine in these cells. The effects of α 2-AR activation were compared with the differentiating action and the signalling mechanisms of nerve growth factor (NGF). Epinephrine induced neuronal differentiation of PC12α 2 cells through α 2-AR activation in a subtype-dependent manner, internalization of all human α 2-AR subtypes, and activation of mitogen-activated protein kinase (MAPK) and the serine-threonine protein kinase Akt. Epinephrine and NGF showed synergism in their differentiating effects. The MAPK kinase (MEK-1) inhibitor PD 98059 abolished the differentiating effect of epinephrine indicating that the differentiation is dependent on MAPK activation. Activating protein-1 (AP-1) DNA-binding activity was increased after epinephrine treatment in all three PC12α 2 subtype clones. Evaluation of the potential physiological consequences of these findings requires further studies on endogenously expressed α 2-ARs in neuronal cells.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0171-9335
fulltext: fulltext
issn:
  • 0171-9335
  • 1618-1298
url: Link


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titleSubtype-specific neuronal differentiation of PC12 cells transfected with α 2-adrenergic receptors
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creatorTaraviras, Stavros ; Olli-Lähdesmäki, Tuire ; Lymperopoulos, Anastasios ; Charitonidou, Despina ; Mavroidis, Manolis ; Kallio, Jaana ; Scheinin, Mika ; Flordellis, Christodoulos
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descriptionCells of the PC12 rat pheochromocytoma cell line acquire characteristics of sympathetic neurons under appropriate treatment. Stably transfected PC12 cells expressing individual α 2-adrenergic receptor (α 2-AR) subtypes were used to assess the role of α 2-ARs in neuronal differentiation and to characterise the signalling pathways activated by the α 2-AR agonist epinephrine in these cells. The effects of α 2-AR activation were compared with the differentiating action and the signalling mechanisms of nerve growth factor (NGF). Epinephrine induced neuronal differentiation of PC12α 2 cells through α 2-AR activation in a subtype-dependent manner, internalization of all human α 2-AR subtypes, and activation of mitogen-activated protein kinase (MAPK) and the serine-threonine protein kinase Akt. Epinephrine and NGF showed synergism in their differentiating effects. The MAPK kinase (MEK-1) inhibitor PD 98059 abolished the differentiating effect of epinephrine indicating that the differentiation is dependent on MAPK activation. Activating protein-1 (AP-1) DNA-binding activity was increased after epinephrine treatment in all three PC12α 2 subtype clones. Evaluation of the potential physiological consequences of these findings requires further studies on endogenously expressed α 2-ARs in neuronal cells.
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abstractCells of the PC12 rat pheochromocytoma cell line acquire characteristics of sympathetic neurons under appropriate treatment. Stably transfected PC12 cells expressing individual α 2-adrenergic receptor (α 2-AR) subtypes were used to assess the role of α 2-ARs in neuronal differentiation and to characterise the signalling pathways activated by the α 2-AR agonist epinephrine in these cells. The effects of α 2-AR activation were compared with the differentiating action and the signalling mechanisms of nerve growth factor (NGF). Epinephrine induced neuronal differentiation of PC12α 2 cells through α 2-AR activation in a subtype-dependent manner, internalization of all human α 2-AR subtypes, and activation of mitogen-activated protein kinase (MAPK) and the serine-threonine protein kinase Akt. Epinephrine and NGF showed synergism in their differentiating effects. The MAPK kinase (MEK-1) inhibitor PD 98059 abolished the differentiating effect of epinephrine indicating that the differentiation is dependent on MAPK activation. Activating protein-1 (AP-1) DNA-binding activity was increased after epinephrine treatment in all three PC12α 2 subtype clones. Evaluation of the potential physiological consequences of these findings requires further studies on endogenously expressed α 2-ARs in neuronal cells.
pubElsevier GmbH
doi10.1078/0171-9335-00250