schliessen

Filtern

 

Bibliotheken

Noninvasive Radiofrequency Field Destruction of Pancreatic Adenocarcinoma Xenografts Treated with Targeted Gold Nanoparticles

Pancreatic carcinoma is one of the deadliest cancers with few effective treatments. Gold nanoparticles (AuNP) are potentially therapeutic because of the safety demonstrated thus far and their physiochemical characteristics. We used the astounding heating rates of AuNPs in nonionizing radiofrequency... Full description

Journal Title: Clinical cancer research 2010, Vol.16 (23), p.5712-5721
Main Author: GLAZER, Evan S
Other Authors: CIHUI ZHU , MASSEY, Katheryn L , SHEA THOMPSON, C , KALUARACHCHI, Warna D , HAMIR, Amir N , CURLEY, Steven A
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Alma/SFX Local Collection
Publisher: Philadelphia, PA: American Association for Cancer Research
ID: ISSN: 1078-0432
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: cdi_gale_infotracacademiconefile_A259467354
title: Noninvasive Radiofrequency Field Destruction of Pancreatic Adenocarcinoma Xenografts Treated with Targeted Gold Nanoparticles
format: Article
creator:
  • GLAZER, Evan S
  • CIHUI ZHU
  • MASSEY, Katheryn L
  • SHEA THOMPSON, C
  • KALUARACHCHI, Warna D
  • HAMIR, Amir N
  • CURLEY, Steven A
subjects:
  • Adenocarcinoma - drug therapy
  • Adenocarcinoma - pathology
  • Adenocarcinoma - radiotherapy
  • Animals
  • Antibodies, Monoclonal - administration & dosage
  • Antibodies, Monoclonal, Humanized
  • Antineoplastic agents
  • Antineoplastic Agents - administration & dosage
  • Apoptosis - drug effects
  • Apoptosis - radiation effects
  • Article
  • Biological and medical sciences
  • cancer
  • Care and treatment
  • Cell Line, Tumor
  • Cetuximab
  • Combined Modality Therapy
  • Diagnosis
  • Drug Delivery Systems - methods
  • Gastroenterology. Liver. Pancreas. Abdomen
  • Gold - administration & dosage
  • Gold - adverse effects
  • Gold - therapeutic use
  • gold nanoparticle
  • Health aspects
  • Humans
  • hyperthermia
  • Liver. Biliary tract. Portal circulation. Exocrine pancreas
  • Medical sciences
  • Metal Nanoparticles - administration & dosage
  • Metal Nanoparticles - adverse effects
  • Metal Nanoparticles - therapeutic use
  • Mice
  • Mice, Inbred BALB C
  • Mice, Nude
  • Nanoparticles
  • nanotechnology
  • Pancreatic cancer
  • Pancreatic Neoplasms - drug therapy
  • Pancreatic Neoplasms - pathology
  • Pancreatic Neoplasms - radiotherapy
  • Pharmacology. Drug treatments
  • Radio Waves - adverse effects
  • Radio Waves - therapeutic use
  • Radiofrequency ablation
  • radiofrequency field therapy
  • Research
  • Tumors
  • Usage
  • Xenograft Model Antitumor Assays
ispartof: Clinical cancer research, 2010, Vol.16 (23), p.5712-5721
description: Pancreatic carcinoma is one of the deadliest cancers with few effective treatments. Gold nanoparticles (AuNP) are potentially therapeutic because of the safety demonstrated thus far and their physiochemical characteristics. We used the astounding heating rates of AuNPs in nonionizing radiofrequency (RF) radiation to investigate human pancreatic xenograft destruction in a murine model. Weekly, Panc-1 and Capan-1 human pancreatic carcinoma xenografts in immunocompromised mice were exposed to an RF field 36 hours after treatment (intraperitoneal) with cetuximab- or PAM4 antibody-conjugated AuNPs, respectively. Tumor sizes were measured weekly, whereas necrosis and cleaved caspase-3 were investigated with hematoxylin-eosin staining and immunofluorescence, respectively. In addition, AuNP internalization and cytotoxicity were investigated in vitro with confocal microscopy and flow cytometry, respectively. Panc-1 cells demonstrated increased apoptosis with decreased viability after treatment with cetuximab-conjugated AuNPs and RF field exposure (P = 0.00005). Differences in xenograft volumes were observed within 2 weeks of initiating therapy. Cetuximab- and PAM4-conjugated AuNPs demonstrated RF field-induced destruction of Panc-1 and Capan-1 pancreatic carcinoma xenografts after 6 weeks of weekly treatment (P = 0.004 and P = 0.035, respectively). There was no evidence of injury to murine organs. Cleaved caspase-3 and necrosis were both increased in treated tumors. This study demonstrates a potentially novel cancer therapy by noninvasively inducing intracellular hyperthermia with targeted AuNPs in an RF field. While the therapy is dependent on the specificity of the targeting antibody, normal tissues were without toxicity despite systemic therapy and whole-body RF field exposure.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 1078-0432
fulltext: fulltext
issn:
  • 1078-0432
  • 0892-7790
  • 1557-3265
  • 1557-900X
url: Link


@attributes
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
RANK2.6409385
LOCALfalse
PrimoNMBib
record
control
sourceidgale_pubme
recordidTN_cdi_gale_infotracacademiconefile_A259467354
sourceformatXML
sourcesystemPC
galeidA259467354
sourcerecordidA259467354
originalsourceidFETCH-LOGICAL-1545t-3ae1c7fa29016e9b26d7402f8552a5b309b378f00bb0835cfee9794979e0a6e83
addsrcrecordideNqFUk2LFDEQbURx19GfoPTFY49Jp5N0IwjDrPsByyrLCN5CdVKZjfQkY9Izsgf_u2lmP1wvEkKqKu-9qqSqKN5SMqeUtx8okW1FGlbPl8vripKqJpw_K44p57JiteDPs32POSpepfSDENpQ0rwsjmpKWduK7rj4fRW883tIbo_lNRgXbMSfO_T6tjx1OJjyBNMYd3p0wZfBll_B64gwOl0uDPqgIWrnwwbK79lbR7BjKlcTAk35y4035QriGifvLGS5K_BhCzHzB0yvixcWhoRv7s5Z8e3082p5Xl1-ObtYLi4ryhs-VgyQammh7ggV2PW1MLIhtW05r4H3jHQ9k60lpO9Jy7i2iJ3smryRgMCWzYpPB93trt-g0ejHCIPaRreBeKsCOPX0xrsbtQ57xQiXPP_grDg_CIQtenARn3CNx1EFo2ohlQUQUre96K2R3AICEgTKZTa0ZjZLzQ9SaxhQOW9DzqjzMrhxOni0LscXNe8aIRmfcvMDQceQUkT7kJsSNc2Cmvqspj6rPAtTdJqFzHv396MfWPfNz4D3dwBIGgYbc2tdesQxkRclGffxnwK0G2EaiFy5G_5Txh-B3tM0
sourcetypeOpen Access Repository
isCDItrue
recordtypearticle
display
typearticle
titleNoninvasive Radiofrequency Field Destruction of Pancreatic Adenocarcinoma Xenografts Treated with Targeted Gold Nanoparticles
sourceAlma/SFX Local Collection
creatorGLAZER, Evan S ; CIHUI ZHU ; MASSEY, Katheryn L ; SHEA THOMPSON, C ; KALUARACHCHI, Warna D ; HAMIR, Amir N ; CURLEY, Steven A
creatorcontribGLAZER, Evan S ; CIHUI ZHU ; MASSEY, Katheryn L ; SHEA THOMPSON, C ; KALUARACHCHI, Warna D ; HAMIR, Amir N ; CURLEY, Steven A
descriptionPancreatic carcinoma is one of the deadliest cancers with few effective treatments. Gold nanoparticles (AuNP) are potentially therapeutic because of the safety demonstrated thus far and their physiochemical characteristics. We used the astounding heating rates of AuNPs in nonionizing radiofrequency (RF) radiation to investigate human pancreatic xenograft destruction in a murine model. Weekly, Panc-1 and Capan-1 human pancreatic carcinoma xenografts in immunocompromised mice were exposed to an RF field 36 hours after treatment (intraperitoneal) with cetuximab- or PAM4 antibody-conjugated AuNPs, respectively. Tumor sizes were measured weekly, whereas necrosis and cleaved caspase-3 were investigated with hematoxylin-eosin staining and immunofluorescence, respectively. In addition, AuNP internalization and cytotoxicity were investigated in vitro with confocal microscopy and flow cytometry, respectively. Panc-1 cells demonstrated increased apoptosis with decreased viability after treatment with cetuximab-conjugated AuNPs and RF field exposure (P = 0.00005). Differences in xenograft volumes were observed within 2 weeks of initiating therapy. Cetuximab- and PAM4-conjugated AuNPs demonstrated RF field-induced destruction of Panc-1 and Capan-1 pancreatic carcinoma xenografts after 6 weeks of weekly treatment (P = 0.004 and P = 0.035, respectively). There was no evidence of injury to murine organs. Cleaved caspase-3 and necrosis were both increased in treated tumors. This study demonstrates a potentially novel cancer therapy by noninvasively inducing intracellular hyperthermia with targeted AuNPs in an RF field. While the therapy is dependent on the specificity of the targeting antibody, normal tissues were without toxicity despite systemic therapy and whole-body RF field exposure.
identifier
0ISSN: 1078-0432
1ISSN: 0892-7790
2EISSN: 1557-3265
3EISSN: 1557-900X
4DOI: 10.1158/1078-0432.CCR-10-2055
5PMID: 21138869
6CODEN: CCREF4
languageeng
publisherPhiladelphia, PA: American Association for Cancer Research
subjectAdenocarcinoma - drug therapy ; Adenocarcinoma - pathology ; Adenocarcinoma - radiotherapy ; Animals ; Antibodies, Monoclonal - administration & dosage ; Antibodies, Monoclonal, Humanized ; Antineoplastic agents ; Antineoplastic Agents - administration & dosage ; Apoptosis - drug effects ; Apoptosis - radiation effects ; Article ; Biological and medical sciences ; cancer ; Care and treatment ; Cell Line, Tumor ; Cetuximab ; Combined Modality Therapy ; Diagnosis ; Drug Delivery Systems - methods ; Gastroenterology. Liver. Pancreas. Abdomen ; Gold - administration & dosage ; Gold - adverse effects ; Gold - therapeutic use ; gold nanoparticle ; Health aspects ; Humans ; hyperthermia ; Liver. Biliary tract. Portal circulation. Exocrine pancreas ; Medical sciences ; Metal Nanoparticles - administration & dosage ; Metal Nanoparticles - adverse effects ; Metal Nanoparticles - therapeutic use ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Nanoparticles ; nanotechnology ; Pancreatic cancer ; Pancreatic Neoplasms - drug therapy ; Pancreatic Neoplasms - pathology ; Pancreatic Neoplasms - radiotherapy ; Pharmacology. Drug treatments ; Radio Waves - adverse effects ; Radio Waves - therapeutic use ; Radiofrequency ablation ; radiofrequency field therapy ; Research ; Tumors ; Usage ; Xenograft Model Antitumor Assays
ispartofClinical cancer research, 2010, Vol.16 (23), p.5712-5721
rights
02015 INIST-CNRS
12010 AACR.
2COPYRIGHT 2011 Mary Ann Liebert, Inc.
lds50peer_reviewed
oafree_for_read
citedbyFETCH-LOGICAL-1545t-3ae1c7fa29016e9b26d7402f8552a5b309b378f00bb0835cfee9794979e0a6e83
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
thumbnail$$Usyndetics_thumb_exl
backlink
0$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23636310$$DView record in Pascal Francis
1$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21138869$$D View this record in MEDLINE/PubMed
search
creatorcontrib
0GLAZER, Evan S
1CIHUI ZHU
2MASSEY, Katheryn L
3SHEA THOMPSON, C
4KALUARACHCHI, Warna D
5HAMIR, Amir N
6CURLEY, Steven A
title
0Noninvasive Radiofrequency Field Destruction of Pancreatic Adenocarcinoma Xenografts Treated with Targeted Gold Nanoparticles
1Clinical cancer research
addtitleClin Cancer Res
descriptionPancreatic carcinoma is one of the deadliest cancers with few effective treatments. Gold nanoparticles (AuNP) are potentially therapeutic because of the safety demonstrated thus far and their physiochemical characteristics. We used the astounding heating rates of AuNPs in nonionizing radiofrequency (RF) radiation to investigate human pancreatic xenograft destruction in a murine model. Weekly, Panc-1 and Capan-1 human pancreatic carcinoma xenografts in immunocompromised mice were exposed to an RF field 36 hours after treatment (intraperitoneal) with cetuximab- or PAM4 antibody-conjugated AuNPs, respectively. Tumor sizes were measured weekly, whereas necrosis and cleaved caspase-3 were investigated with hematoxylin-eosin staining and immunofluorescence, respectively. In addition, AuNP internalization and cytotoxicity were investigated in vitro with confocal microscopy and flow cytometry, respectively. Panc-1 cells demonstrated increased apoptosis with decreased viability after treatment with cetuximab-conjugated AuNPs and RF field exposure (P = 0.00005). Differences in xenograft volumes were observed within 2 weeks of initiating therapy. Cetuximab- and PAM4-conjugated AuNPs demonstrated RF field-induced destruction of Panc-1 and Capan-1 pancreatic carcinoma xenografts after 6 weeks of weekly treatment (P = 0.004 and P = 0.035, respectively). There was no evidence of injury to murine organs. Cleaved caspase-3 and necrosis were both increased in treated tumors. This study demonstrates a potentially novel cancer therapy by noninvasively inducing intracellular hyperthermia with targeted AuNPs in an RF field. While the therapy is dependent on the specificity of the targeting antibody, normal tissues were without toxicity despite systemic therapy and whole-body RF field exposure.
subject
0Adenocarcinoma - drug therapy
1Adenocarcinoma - pathology
2Adenocarcinoma - radiotherapy
3Animals
4Antibodies, Monoclonal - administration & dosage
5Antibodies, Monoclonal, Humanized
6Antineoplastic agents
7Antineoplastic Agents - administration & dosage
8Apoptosis - drug effects
9Apoptosis - radiation effects
10Article
11Biological and medical sciences
12cancer
13Care and treatment
14Cell Line, Tumor
15Cetuximab
16Combined Modality Therapy
17Diagnosis
18Drug Delivery Systems - methods
19Gastroenterology. Liver. Pancreas. Abdomen
20Gold - administration & dosage
21Gold - adverse effects
22Gold - therapeutic use
23gold nanoparticle
24Health aspects
25Humans
26hyperthermia
27Liver. Biliary tract. Portal circulation. Exocrine pancreas
28Medical sciences
29Metal Nanoparticles - administration & dosage
30Metal Nanoparticles - adverse effects
31Metal Nanoparticles - therapeutic use
32Mice
33Mice, Inbred BALB C
34Mice, Nude
35Nanoparticles
36nanotechnology
37Pancreatic cancer
38Pancreatic Neoplasms - drug therapy
39Pancreatic Neoplasms - pathology
40Pancreatic Neoplasms - radiotherapy
41Pharmacology. Drug treatments
42Radio Waves - adverse effects
43Radio Waves - therapeutic use
44Radiofrequency ablation
45radiofrequency field therapy
46Research
47Tumors
48Usage
49Xenograft Model Antitumor Assays
issn
01078-0432
10892-7790
21557-3265
31557-900X
fulltexttrue
rsrctypearticle
creationdate2010
recordtypearticle
recordideNqFUk2LFDEQbURx19GfoPTFY49Jp5N0IwjDrPsByyrLCN5CdVKZjfQkY9Izsgf_u2lmP1wvEkKqKu-9qqSqKN5SMqeUtx8okW1FGlbPl8vripKqJpw_K44p57JiteDPs32POSpepfSDENpQ0rwsjmpKWduK7rj4fRW883tIbo_lNRgXbMSfO_T6tjx1OJjyBNMYd3p0wZfBll_B64gwOl0uDPqgIWrnwwbK79lbR7BjKlcTAk35y4035QriGifvLGS5K_BhCzHzB0yvixcWhoRv7s5Z8e3082p5Xl1-ObtYLi4ryhs-VgyQammh7ggV2PW1MLIhtW05r4H3jHQ9k60lpO9Jy7i2iJ3smryRgMCWzYpPB93trt-g0ejHCIPaRreBeKsCOPX0xrsbtQ57xQiXPP_grDg_CIQtenARn3CNx1EFo2ohlQUQUre96K2R3AICEgTKZTa0ZjZLzQ9SaxhQOW9DzqjzMrhxOni0LscXNe8aIRmfcvMDQceQUkT7kJsSNc2Cmvqspj6rPAtTdJqFzHv396MfWPfNz4D3dwBIGgYbc2tdesQxkRclGffxnwK0G2EaiFy5G_5Txh-B3tM0
startdate2010
enddate2010
creator
0GLAZER, Evan S
1CIHUI ZHU
2MASSEY, Katheryn L
3SHEA THOMPSON, C
4KALUARACHCHI, Warna D
5HAMIR, Amir N
6CURLEY, Steven A
general
0American Association for Cancer Research
1Mary Ann Liebert, Inc
scope
0IQODW
1CGR
2CUY
3CVF
4ECM
5EIF
6NPM
7AAYXX
8CITATION
9BOBZL
10CLFQK
115PM
sort
creationdate2010
titleNoninvasive Radiofrequency Field Destruction of Pancreatic Adenocarcinoma Xenografts Treated with Targeted Gold Nanoparticles
authorGLAZER, Evan S ; CIHUI ZHU ; MASSEY, Katheryn L ; SHEA THOMPSON, C ; KALUARACHCHI, Warna D ; HAMIR, Amir N ; CURLEY, Steven A
facets
frbrtype5
frbrgroupidcdi_FETCH-LOGICAL-1545t-3ae1c7fa29016e9b26d7402f8552a5b309b378f00bb0835cfee9794979e0a6e83
rsrctypearticles
prefilterarticles
languageeng
creationdate2010
topic
0Adenocarcinoma - drug therapy
1Adenocarcinoma - pathology
2Adenocarcinoma - radiotherapy
3Animals
4Antibodies, Monoclonal - administration & dosage
5Antibodies, Monoclonal, Humanized
6Antineoplastic agents
7Antineoplastic Agents - administration & dosage
8Apoptosis - drug effects
9Apoptosis - radiation effects
10Article
11Biological and medical sciences
12cancer
13Care and treatment
14Cell Line, Tumor
15Cetuximab
16Combined Modality Therapy
17Diagnosis
18Drug Delivery Systems - methods
19Gastroenterology. Liver. Pancreas. Abdomen
20Gold - administration & dosage
21Gold - adverse effects
22Gold - therapeutic use
23gold nanoparticle
24Health aspects
25Humans
26hyperthermia
27Liver. Biliary tract. Portal circulation. Exocrine pancreas
28Medical sciences
29Metal Nanoparticles - administration & dosage
30Metal Nanoparticles - adverse effects
31Metal Nanoparticles - therapeutic use
32Mice
33Mice, Inbred BALB C
34Mice, Nude
35Nanoparticles
36nanotechnology
37Pancreatic cancer
38Pancreatic Neoplasms - drug therapy
39Pancreatic Neoplasms - pathology
40Pancreatic Neoplasms - radiotherapy
41Pharmacology. Drug treatments
42Radio Waves - adverse effects
43Radio Waves - therapeutic use
44Radiofrequency ablation
45radiofrequency field therapy
46Research
47Tumors
48Usage
49Xenograft Model Antitumor Assays
toplevel
0peer_reviewed
1online_resources
creatorcontrib
0GLAZER, Evan S
1CIHUI ZHU
2MASSEY, Katheryn L
3SHEA THOMPSON, C
4KALUARACHCHI, Warna D
5HAMIR, Amir N
6CURLEY, Steven A
collection
0Pascal-Francis
1Medline
2MEDLINE
3MEDLINE (Ovid)
4MEDLINE
5MEDLINE
6PubMed
7CrossRef
8OpenAIRE (Open Access)
9OpenAIRE
10PubMed Central (Full Participant titles)
jtitleClinical cancer research
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
au
0GLAZER, Evan S
1CIHUI ZHU
2MASSEY, Katheryn L
3SHEA THOMPSON, C
4KALUARACHCHI, Warna D
5HAMIR, Amir N
6CURLEY, Steven A
formatjournal
genrearticle
ristypeJOUR
atitleNoninvasive Radiofrequency Field Destruction of Pancreatic Adenocarcinoma Xenografts Treated with Targeted Gold Nanoparticles
jtitleClinical cancer research
addtitleClin Cancer Res
date2010
risdate2010
volume16
issue23
spage5712
epage5721
pages5712-5721
issn
01078-0432
10892-7790
eissn
01557-3265
11557-900X
codenCCREF4
abstractPancreatic carcinoma is one of the deadliest cancers with few effective treatments. Gold nanoparticles (AuNP) are potentially therapeutic because of the safety demonstrated thus far and their physiochemical characteristics. We used the astounding heating rates of AuNPs in nonionizing radiofrequency (RF) radiation to investigate human pancreatic xenograft destruction in a murine model. Weekly, Panc-1 and Capan-1 human pancreatic carcinoma xenografts in immunocompromised mice were exposed to an RF field 36 hours after treatment (intraperitoneal) with cetuximab- or PAM4 antibody-conjugated AuNPs, respectively. Tumor sizes were measured weekly, whereas necrosis and cleaved caspase-3 were investigated with hematoxylin-eosin staining and immunofluorescence, respectively. In addition, AuNP internalization and cytotoxicity were investigated in vitro with confocal microscopy and flow cytometry, respectively. Panc-1 cells demonstrated increased apoptosis with decreased viability after treatment with cetuximab-conjugated AuNPs and RF field exposure (P = 0.00005). Differences in xenograft volumes were observed within 2 weeks of initiating therapy. Cetuximab- and PAM4-conjugated AuNPs demonstrated RF field-induced destruction of Panc-1 and Capan-1 pancreatic carcinoma xenografts after 6 weeks of weekly treatment (P = 0.004 and P = 0.035, respectively). There was no evidence of injury to murine organs. Cleaved caspase-3 and necrosis were both increased in treated tumors. This study demonstrates a potentially novel cancer therapy by noninvasively inducing intracellular hyperthermia with targeted AuNPs in an RF field. While the therapy is dependent on the specificity of the targeting antibody, normal tissues were without toxicity despite systemic therapy and whole-body RF field exposure.
copPhiladelphia, PA
pubAmerican Association for Cancer Research
pmid21138869
doi10.1158/1078-0432.CCR-10-2055
oafree_for_read