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Necrotizing Enterocolitis in Preterm Pigs Is Associated with Increased Density of Intestinal Mucosa-Associated Bacteria Including Clostridium perfringens

Background: Necrotizing enterocolitis (NEC) is associated with changes in the luminal gut microbiota. It is not known whether the mucosa-associated microbiota is affected by NEC and stimulates inflammatory lesions. Objective: We hypothesized that the density of the mucosa-associated microbiota corre... Full description

Journal Title: Neonatology (Basel Switzerland), 2015, Vol.108 (3), p.188-195
Main Author: Støy, Ann Cathrine F
Other Authors: Mølbak, Lars , Delègue, Camilla L , Thymann, Thomas , Sangild, Per T , Heegaard, Peter M.H , Manurung, Sarmauli , Skovgaard, Kerstin
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Alma/SFX Local Collection
Publisher: Basel, Switzerland: S. Karger AG
ID: ISSN: 1661-7800
Link: https://www.ncbi.nlm.nih.gov/pubmed/26278554
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title: Necrotizing Enterocolitis in Preterm Pigs Is Associated with Increased Density of Intestinal Mucosa-Associated Bacteria Including Clostridium perfringens
format: Article
creator:
  • Støy, Ann Cathrine F
  • Mølbak, Lars
  • Delègue, Camilla L
  • Thymann, Thomas
  • Sangild, Per T
  • Heegaard, Peter M.H
  • Manurung, Sarmauli
  • Skovgaard, Kerstin
subjects:
  • Animals
  • Animals, Newborn
  • Causes of
  • Cell Line
  • Cesarean Section
  • Clostridium infections
  • Clostridium Infections - genetics
  • Clostridium Infections - microbiology
  • Clostridium perfringens - genetics
  • Complications and side effects
  • digestive system diseases
  • Enterocolitis, Necrotizing - microbiology
  • Enterocolitis, Neonatal necrotizing
  • Enterocolitis, Pseudomembranous
  • Female
  • Gene expression
  • Genetic aspects
  • Health aspects
  • Infants (Premature)
  • Intestinal mucosa
  • Intestinal Mucosa - microbiology
  • Microbiota
  • Microbiota (Symbiotic organisms)
  • Models
  • Original Paper
  • Pediatric research
  • Pregnancy
  • Premature Birth
  • Swine
ispartof: Neonatology (Basel, Switzerland), 2015, Vol.108 (3), p.188-195
description: Background: Necrotizing enterocolitis (NEC) is associated with changes in the luminal gut microbiota. It is not known whether the mucosa-associated microbiota is affected by NEC and stimulates inflammatory lesions. Objective: We hypothesized that the density of the mucosa-associated microbiota correlates with NEC severity in preterm pigs and that in vitro infection with increasing densities of Clostridium perfringens, which has been associated with NEC in preterm infants, would lead to a transcriptional response related to the inflammatory conditions of NEC. Methods: First, we determined the density of total bacteria and C. perfringens in the distal small intestinal mucosa of 58 NEC and healthy preterm pigs using quantitative PCR. Next, we analyzed in IPEC-J2 cells the effect of different infection densities of C. perfringens type A on the expression of genes related to intestinal function and immune response. Results: Total bacterial and C. perfringens densities were higher in NEC versus healthy pigs and correlated positively with NEC severity. In IPEC-J2 cells expression levels of inflammation-related genes (CCL5, NFKBIA, IL8, IL1RN, and TNFAIP3) increased, while the expression of the sodium/glucose co-transporter (SLC5A1) decreased, with increasing density of C. perfringens. Conclusions: Total bacterial and C. perfringens densities were higher in NEC versus healthy pigs and correlated positively with NEC severity. In IPEC-J2 cells expression levels of inflammation-related genes (CCL5, NFKBIA, IL8, IL1RN, and TNFAIP3) increased, while the expression of the sodium/glucose co-transporter (SLC5A1) decreased, with increasing density of C. perfringens.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 1661-7800
fulltext: fulltext
issn:
  • 1661-7800
  • 1661-7819
url: Link


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titleNecrotizing Enterocolitis in Preterm Pigs Is Associated with Increased Density of Intestinal Mucosa-Associated Bacteria Including Clostridium perfringens
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creatorStøy, Ann Cathrine F ; Mølbak, Lars ; Delègue, Camilla L ; Thymann, Thomas ; Sangild, Per T ; Heegaard, Peter M.H ; Manurung, Sarmauli ; Skovgaard, Kerstin
creatorcontribStøy, Ann Cathrine F ; Mølbak, Lars ; Delègue, Camilla L ; Thymann, Thomas ; Sangild, Per T ; Heegaard, Peter M.H ; Manurung, Sarmauli ; Skovgaard, Kerstin
descriptionBackground: Necrotizing enterocolitis (NEC) is associated with changes in the luminal gut microbiota. It is not known whether the mucosa-associated microbiota is affected by NEC and stimulates inflammatory lesions. Objective: We hypothesized that the density of the mucosa-associated microbiota correlates with NEC severity in preterm pigs and that in vitro infection with increasing densities of Clostridium perfringens, which has been associated with NEC in preterm infants, would lead to a transcriptional response related to the inflammatory conditions of NEC. Methods: First, we determined the density of total bacteria and C. perfringens in the distal small intestinal mucosa of 58 NEC and healthy preterm pigs using quantitative PCR. Next, we analyzed in IPEC-J2 cells the effect of different infection densities of C. perfringens type A on the expression of genes related to intestinal function and immune response. Results: Total bacterial and C. perfringens densities were higher in NEC versus healthy pigs and correlated positively with NEC severity. In IPEC-J2 cells expression levels of inflammation-related genes (CCL5, NFKBIA, IL8, IL1RN, and TNFAIP3) increased, while the expression of the sodium/glucose co-transporter (SLC5A1) decreased, with increasing density of C. perfringens. Conclusions: Total bacterial and C. perfringens densities were higher in NEC versus healthy pigs and correlated positively with NEC severity. In IPEC-J2 cells expression levels of inflammation-related genes (CCL5, NFKBIA, IL8, IL1RN, and TNFAIP3) increased, while the expression of the sodium/glucose co-transporter (SLC5A1) decreased, with increasing density of C. perfringens.
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subjectAnimals ; Animals, Newborn ; Causes of ; Cell Line ; Cesarean Section ; Clostridium infections ; Clostridium Infections - genetics ; Clostridium Infections - microbiology ; Clostridium perfringens - genetics ; Complications and side effects ; digestive system diseases ; Enterocolitis, Necrotizing - microbiology ; Enterocolitis, Neonatal necrotizing ; Enterocolitis, Pseudomembranous ; Female ; Gene expression ; Genetic aspects ; Health aspects ; Infants (Premature) ; Intestinal mucosa ; Intestinal Mucosa - microbiology ; Microbiota ; Microbiota (Symbiotic organisms) ; Models ; Original Paper ; Pediatric research ; Pregnancy ; Premature Birth ; Swine
ispartofNeonatology (Basel, Switzerland), 2015, Vol.108 (3), p.188-195
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2Delègue, Camilla L
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7Skovgaard, Kerstin
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descriptionBackground: Necrotizing enterocolitis (NEC) is associated with changes in the luminal gut microbiota. It is not known whether the mucosa-associated microbiota is affected by NEC and stimulates inflammatory lesions. Objective: We hypothesized that the density of the mucosa-associated microbiota correlates with NEC severity in preterm pigs and that in vitro infection with increasing densities of Clostridium perfringens, which has been associated with NEC in preterm infants, would lead to a transcriptional response related to the inflammatory conditions of NEC. Methods: First, we determined the density of total bacteria and C. perfringens in the distal small intestinal mucosa of 58 NEC and healthy preterm pigs using quantitative PCR. Next, we analyzed in IPEC-J2 cells the effect of different infection densities of C. perfringens type A on the expression of genes related to intestinal function and immune response. Results: Total bacterial and C. perfringens densities were higher in NEC versus healthy pigs and correlated positively with NEC severity. In IPEC-J2 cells expression levels of inflammation-related genes (CCL5, NFKBIA, IL8, IL1RN, and TNFAIP3) increased, while the expression of the sodium/glucose co-transporter (SLC5A1) decreased, with increasing density of C. perfringens. Conclusions: Total bacterial and C. perfringens densities were higher in NEC versus healthy pigs and correlated positively with NEC severity. In IPEC-J2 cells expression levels of inflammation-related genes (CCL5, NFKBIA, IL8, IL1RN, and TNFAIP3) increased, while the expression of the sodium/glucose co-transporter (SLC5A1) decreased, with increasing density of C. perfringens.
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titleNecrotizing Enterocolitis in Preterm Pigs Is Associated with Increased Density of Intestinal Mucosa-Associated Bacteria Including Clostridium perfringens
authorStøy, Ann Cathrine F ; Mølbak, Lars ; Delègue, Camilla L ; Thymann, Thomas ; Sangild, Per T ; Heegaard, Peter M.H ; Manurung, Sarmauli ; Skovgaard, Kerstin
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abstractBackground: Necrotizing enterocolitis (NEC) is associated with changes in the luminal gut microbiota. It is not known whether the mucosa-associated microbiota is affected by NEC and stimulates inflammatory lesions. Objective: We hypothesized that the density of the mucosa-associated microbiota correlates with NEC severity in preterm pigs and that in vitro infection with increasing densities of Clostridium perfringens, which has been associated with NEC in preterm infants, would lead to a transcriptional response related to the inflammatory conditions of NEC. Methods: First, we determined the density of total bacteria and C. perfringens in the distal small intestinal mucosa of 58 NEC and healthy preterm pigs using quantitative PCR. Next, we analyzed in IPEC-J2 cells the effect of different infection densities of C. perfringens type A on the expression of genes related to intestinal function and immune response. Results: Total bacterial and C. perfringens densities were higher in NEC versus healthy pigs and correlated positively with NEC severity. In IPEC-J2 cells expression levels of inflammation-related genes (CCL5, NFKBIA, IL8, IL1RN, and TNFAIP3) increased, while the expression of the sodium/glucose co-transporter (SLC5A1) decreased, with increasing density of C. perfringens. Conclusions: Total bacterial and C. perfringens densities were higher in NEC versus healthy pigs and correlated positively with NEC severity. In IPEC-J2 cells expression levels of inflammation-related genes (CCL5, NFKBIA, IL8, IL1RN, and TNFAIP3) increased, while the expression of the sodium/glucose co-transporter (SLC5A1) decreased, with increasing density of C. perfringens.
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