schliessen

Filtern

 

Bibliotheken

Direct detection of extended-spectrum-[beta]-lactamase-producers in Enterobacterales from blood cultures: a comparative analysis

Keywords: ESBL; CTX-M; Bloodstream infection; Sepsis; Gram-negatives; Rapid diagnostic test Accurate detection of extended-spectrum-[beta]-lactamase (ESBL)-producing Enterobacterales from bloodstream infection (BSI) is of paramount importance for both epidemiological and clinical purposes, especiall... Full description

Journal Title: European journal of clinical microbiology & infectious diseases 2022-03-01, Vol.41 (3), p.407
Main Author: Boattini, Matteo
Other Authors: Bianco, Gabriele , Comini, Sara , Iannaccone, Marco , Casale, Roberto , Cavallo, Rossana , Nordmann, Patrice
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: Springer
ID: ISSN: 0934-9723
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: cdi_gale_infotracacademiconefile_A693091837
title: Direct detection of extended-spectrum-[beta]-lactamase-producers in Enterobacterales from blood cultures: a comparative analysis
format: Article
creator:
  • Boattini, Matteo
  • Bianco, Gabriele
  • Comini, Sara
  • Iannaccone, Marco
  • Casale, Roberto
  • Cavallo, Rossana
  • Nordmann, Patrice
subjects:
  • Bacterial pneumonia
  • Beta lactamases
  • Blood
  • Comparative analysis
  • Drug resistance in microorganisms
  • Epidemiology
  • Escherichia coli
  • Medical examination
  • Pneumonia
ispartof: European journal of clinical microbiology & infectious diseases, 2022-03-01, Vol.41 (3), p.407
description: Keywords: ESBL; CTX-M; Bloodstream infection; Sepsis; Gram-negatives; Rapid diagnostic test Accurate detection of extended-spectrum-[beta]-lactamase (ESBL)-producing Enterobacterales from bloodstream infection (BSI) is of paramount importance for both epidemiological and clinical purposes, especially for optimization of antibiotic stewardship interventions. Three phenotypic methods for the detection of ESBL phenotype in Klebsiella pneumoniae and Escherichia coli BSI were compared over a 4-month period (May--August 2021) in a main University Hospital from Northern Italy. The methods were the biochemical Rapid ESBL NP®, the immunological NG-Test CTX-M MULTI®, and the E-test technique based on ESBL E-test®. One hundred forty-two blood cultures (BCs) positive for K. pneumoniae or E. coli were included. ESBL and carbapenemase phenotype were detected in 26.1% (n=37) and 16.9% (n=24), respectively. The Rapid ESBL NP®, NG-Test CTX-M MULTI®, and direct ESBL E-test® positive and negative predictive values with 95% confidence intervals were 1 (0.87--1) and 0.97 (0.92--0.99), 1 (0.87--1) and 0.97 (0.92--0.99), and 1 (0.88--1) and 1 (0.96--1), respectively. The three phenotypic methods evaluated showed good performance in the detection of ESBL phenotype from K. pneumoniae-- or E. coli--positive BCs. Rapid ESBL NP® and NG-test CTX-M® offer the important advantage of a turnaround time of 15 to 45 min, and the Rapid ESBL NP test in addition detects any type of ESBL producers. Author Affiliation: (1) Microbiology and Virology Unit, University Hospital Città Della Salute E Della Scienza Di Torino, Corso Bramante 88/90, 10126, Turin, Italy (2) Department of Public Health and Pediatric Sciences, University of Turin, Turin, Italy (3) Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland (4) Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland (5) INSERM European Unit (IAME), University of Fribourg, Fribourg, Switzerland (6) Institute for Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland (a) matteo.boattini@unito.it Article History: Registration Date: 11/22/2021 Received Date: 10/17/2021 Accepted Date: 11/19/2021 Online Date: 11/25/2021 Byline:
language: eng
source:
identifier: ISSN: 0934-9723
fulltext: no_fulltext
issn:
  • 0934-9723
  • 1435-4373
url: Link


@attributes
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
RANK2.7126591
LOCALfalse
PrimoNMBib
record
control
sourceidgale
recordidTN_cdi_gale_infotracacademiconefile_A693091837
sourceformatXML
sourcesystemPC
galeidA693091837
sourcerecordidA693091837
originalsourceidFETCH-gale_infotracacademiconefile_A6930918370
addsrcrecordideNqVjE1KBDEUhIMo2P5cwNW7QMZk0jPpdic6MgdwJyKvkxeJpJMmSYvuPLpZeAEpqIKvqGLsRoqNFELflubjnout5KJXw47LE9bJXu14r7Q6ZZ0YVc9HvVXn7KKUD9FGg9Yd-3n0mUwFS7WFTxGSA_qqFC1ZXpYG8zrzl4kqvvKApuKMhfiSk10N5QI-wiFWymlqJWUMVMDlNMMUUrJg1lDXTOUOEEyaF8xY_ScBRgzfxZcrduYwFLr-y0u2eTo8Pxz5e3t689GlmtE0WZq9SZGcb_x-PyoxykFp8e_BL0KKYR8
sourcetypeAggregation Database
isCDItrue
recordtypearticle
display
typearticle
titleDirect detection of extended-spectrum-[beta]-lactamase-producers in Enterobacterales from blood cultures: a comparative analysis
creatorBoattini, Matteo ; Bianco, Gabriele ; Comini, Sara ; Iannaccone, Marco ; Casale, Roberto ; Cavallo, Rossana ; Nordmann, Patrice
creatorcontribBoattini, Matteo ; Bianco, Gabriele ; Comini, Sara ; Iannaccone, Marco ; Casale, Roberto ; Cavallo, Rossana ; Nordmann, Patrice
descriptionKeywords: ESBL; CTX-M; Bloodstream infection; Sepsis; Gram-negatives; Rapid diagnostic test Accurate detection of extended-spectrum-[beta]-lactamase (ESBL)-producing Enterobacterales from bloodstream infection (BSI) is of paramount importance for both epidemiological and clinical purposes, especially for optimization of antibiotic stewardship interventions. Three phenotypic methods for the detection of ESBL phenotype in Klebsiella pneumoniae and Escherichia coli BSI were compared over a 4-month period (May--August 2021) in a main University Hospital from Northern Italy. The methods were the biochemical Rapid ESBL NP®, the immunological NG-Test CTX-M MULTI®, and the E-test technique based on ESBL E-test®. One hundred forty-two blood cultures (BCs) positive for K. pneumoniae or E. coli were included. ESBL and carbapenemase phenotype were detected in 26.1% (n=37) and 16.9% (n=24), respectively. The Rapid ESBL NP®, NG-Test CTX-M MULTI®, and direct ESBL E-test® positive and negative predictive values with 95% confidence intervals were 1 (0.87--1) and 0.97 (0.92--0.99), 1 (0.87--1) and 0.97 (0.92--0.99), and 1 (0.88--1) and 1 (0.96--1), respectively. The three phenotypic methods evaluated showed good performance in the detection of ESBL phenotype from K. pneumoniae-- or E. coli--positive BCs. Rapid ESBL NP® and NG-test CTX-M® offer the important advantage of a turnaround time of 15 to 45 min, and the Rapid ESBL NP test in addition detects any type of ESBL producers. Author Affiliation: (1) Microbiology and Virology Unit, University Hospital Città Della Salute E Della Scienza Di Torino, Corso Bramante 88/90, 10126, Turin, Italy (2) Department of Public Health and Pediatric Sciences, University of Turin, Turin, Italy (3) Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland (4) Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland (5) INSERM European Unit (IAME), University of Fribourg, Fribourg, Switzerland (6) Institute for Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland (a) matteo.boattini@unito.it Article History: Registration Date: 11/22/2021 Received Date: 10/17/2021 Accepted Date: 11/19/2021 Online Date: 11/25/2021 Byline:
identifier
0ISSN: 0934-9723
1EISSN: 1435-4373
2DOI: 10.1007/s10096-021-04385-1
languageeng
publisherSpringer
subjectBacterial pneumonia ; Beta lactamases ; Blood ; Comparative analysis ; Drug resistance in microorganisms ; Epidemiology ; Escherichia coli ; Medical examination ; Pneumonia
ispartofEuropean journal of clinical microbiology & infectious diseases, 2022-03-01, Vol.41 (3), p.407
rightsCOPYRIGHT 2022 Springer
lds50peer_reviewed
links
openurl$$Topenurl_article
thumbnail$$Usyndetics_thumb_exl
search
creatorcontrib
0Boattini, Matteo
1Bianco, Gabriele
2Comini, Sara
3Iannaccone, Marco
4Casale, Roberto
5Cavallo, Rossana
6Nordmann, Patrice
title
0Direct detection of extended-spectrum-[beta]-lactamase-producers in Enterobacterales from blood cultures: a comparative analysis
1European journal of clinical microbiology & infectious diseases
descriptionKeywords: ESBL; CTX-M; Bloodstream infection; Sepsis; Gram-negatives; Rapid diagnostic test Accurate detection of extended-spectrum-[beta]-lactamase (ESBL)-producing Enterobacterales from bloodstream infection (BSI) is of paramount importance for both epidemiological and clinical purposes, especially for optimization of antibiotic stewardship interventions. Three phenotypic methods for the detection of ESBL phenotype in Klebsiella pneumoniae and Escherichia coli BSI were compared over a 4-month period (May--August 2021) in a main University Hospital from Northern Italy. The methods were the biochemical Rapid ESBL NP®, the immunological NG-Test CTX-M MULTI®, and the E-test technique based on ESBL E-test®. One hundred forty-two blood cultures (BCs) positive for K. pneumoniae or E. coli were included. ESBL and carbapenemase phenotype were detected in 26.1% (n=37) and 16.9% (n=24), respectively. The Rapid ESBL NP®, NG-Test CTX-M MULTI®, and direct ESBL E-test® positive and negative predictive values with 95% confidence intervals were 1 (0.87--1) and 0.97 (0.92--0.99), 1 (0.87--1) and 0.97 (0.92--0.99), and 1 (0.88--1) and 1 (0.96--1), respectively. The three phenotypic methods evaluated showed good performance in the detection of ESBL phenotype from K. pneumoniae-- or E. coli--positive BCs. Rapid ESBL NP® and NG-test CTX-M® offer the important advantage of a turnaround time of 15 to 45 min, and the Rapid ESBL NP test in addition detects any type of ESBL producers. Author Affiliation: (1) Microbiology and Virology Unit, University Hospital Città Della Salute E Della Scienza Di Torino, Corso Bramante 88/90, 10126, Turin, Italy (2) Department of Public Health and Pediatric Sciences, University of Turin, Turin, Italy (3) Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland (4) Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland (5) INSERM European Unit (IAME), University of Fribourg, Fribourg, Switzerland (6) Institute for Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland (a) matteo.boattini@unito.it Article History: Registration Date: 11/22/2021 Received Date: 10/17/2021 Accepted Date: 11/19/2021 Online Date: 11/25/2021 Byline:
subject
0Bacterial pneumonia
1Beta lactamases
2Blood
3Comparative analysis
4Drug resistance in microorganisms
5Epidemiology
6Escherichia coli
7Medical examination
8Pneumonia
issn
00934-9723
11435-4373
fulltextfalse
rsrctypearticle
creationdate2022
recordtypearticle
recordideNqVjE1KBDEUhIMo2P5cwNW7QMZk0jPpdic6MgdwJyKvkxeJpJMmSYvuPLpZeAEpqIKvqGLsRoqNFELflubjnout5KJXw47LE9bJXu14r7Q6ZZ0YVc9HvVXn7KKUD9FGg9Yd-3n0mUwFS7WFTxGSA_qqFC1ZXpYG8zrzl4kqvvKApuKMhfiSk10N5QI-wiFWymlqJWUMVMDlNMMUUrJg1lDXTOUOEEyaF8xY_ScBRgzfxZcrduYwFLr-y0u2eTo8Pxz5e3t689GlmtE0WZq9SZGcb_x-PyoxykFp8e_BL0KKYR8
startdate20220301
enddate20220301
creator
0Boattini, Matteo
1Bianco, Gabriele
2Comini, Sara
3Iannaccone, Marco
4Casale, Roberto
5Cavallo, Rossana
6Nordmann, Patrice
generalSpringer
scopeBSHEE
sort
creationdate20220301
titleDirect detection of extended-spectrum-[beta]-lactamase-producers in Enterobacterales from blood cultures: a comparative analysis
authorBoattini, Matteo ; Bianco, Gabriele ; Comini, Sara ; Iannaccone, Marco ; Casale, Roberto ; Cavallo, Rossana ; Nordmann, Patrice
facets
frbrtype5
frbrgroupidcdi_FETCH-gale_infotracacademiconefile_A6930918370
rsrctypearticles
prefilterarticles
languageeng
creationdate2022
topic
0Bacterial pneumonia
1Beta lactamases
2Blood
3Comparative analysis
4Drug resistance in microorganisms
5Epidemiology
6Escherichia coli
7Medical examination
8Pneumonia
toplevelpeer_reviewed
creatorcontrib
0Boattini, Matteo
1Bianco, Gabriele
2Comini, Sara
3Iannaccone, Marco
4Casale, Roberto
5Cavallo, Rossana
6Nordmann, Patrice
collectionAcademic OneFile (A&I only)
jtitleEuropean journal of clinical microbiology & infectious diseases
delivery
delcategoryRemote Search Resource
fulltextno_fulltext
addata
au
0Boattini, Matteo
1Bianco, Gabriele
2Comini, Sara
3Iannaccone, Marco
4Casale, Roberto
5Cavallo, Rossana
6Nordmann, Patrice
formatjournal
genrearticle
ristypeJOUR
atitleDirect detection of extended-spectrum-[beta]-lactamase-producers in Enterobacterales from blood cultures: a comparative analysis
jtitleEuropean journal of clinical microbiology & infectious diseases
date2022-03-01
risdate2022
volume41
issue3
spage407
pages407-
issn0934-9723
eissn1435-4373
abstractKeywords: ESBL; CTX-M; Bloodstream infection; Sepsis; Gram-negatives; Rapid diagnostic test Accurate detection of extended-spectrum-[beta]-lactamase (ESBL)-producing Enterobacterales from bloodstream infection (BSI) is of paramount importance for both epidemiological and clinical purposes, especially for optimization of antibiotic stewardship interventions. Three phenotypic methods for the detection of ESBL phenotype in Klebsiella pneumoniae and Escherichia coli BSI were compared over a 4-month period (May--August 2021) in a main University Hospital from Northern Italy. The methods were the biochemical Rapid ESBL NP®, the immunological NG-Test CTX-M MULTI®, and the E-test technique based on ESBL E-test®. One hundred forty-two blood cultures (BCs) positive for K. pneumoniae or E. coli were included. ESBL and carbapenemase phenotype were detected in 26.1% (n=37) and 16.9% (n=24), respectively. The Rapid ESBL NP®, NG-Test CTX-M MULTI®, and direct ESBL E-test® positive and negative predictive values with 95% confidence intervals were 1 (0.87--1) and 0.97 (0.92--0.99), 1 (0.87--1) and 0.97 (0.92--0.99), and 1 (0.88--1) and 1 (0.96--1), respectively. The three phenotypic methods evaluated showed good performance in the detection of ESBL phenotype from K. pneumoniae-- or E. coli--positive BCs. Rapid ESBL NP® and NG-test CTX-M® offer the important advantage of a turnaround time of 15 to 45 min, and the Rapid ESBL NP test in addition detects any type of ESBL producers. Author Affiliation: (1) Microbiology and Virology Unit, University Hospital Città Della Salute E Della Scienza Di Torino, Corso Bramante 88/90, 10126, Turin, Italy (2) Department of Public Health and Pediatric Sciences, University of Turin, Turin, Italy (3) Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland (4) Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland (5) INSERM European Unit (IAME), University of Fribourg, Fribourg, Switzerland (6) Institute for Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland (a) matteo.boattini@unito.it Article History: Registration Date: 11/22/2021 Received Date: 10/17/2021 Accepted Date: 11/19/2021 Online Date: 11/25/2021 Byline:
pubSpringer
doi10.1007/s10096-021-04385-1