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AVP dynamically increases paracellular Na.sup.+ permeability and transcellular NaCl transport in the medullary thick ascending limb of Henle's loop

Byline: Nina Himmerkus (1), Allein Plain (1), Rita D. Marques (2), Svenja R. Sonntag (1), Alexander Paliege (3), Jens Leipziger (2), Markus Bleich (1) Keywords: Claudins; Concentration mechanism; Outer medulla; Tight junction; Sodium transport The medullary thick ascending limb of Henle's loop (mTAL... Full description

Journal Title: Pflügers Archiv 2017, Vol.469 (1), p.149
Main Author: Himmerkus, Nina
Other Authors: Plain, Allein , Marques, Rita D , Sonntag, Svenja R , Paliege, Alexander , Leipziger, Jens , Bleich, Markus
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: Springer
ID: ISSN: 0031-6768
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title: AVP dynamically increases paracellular Na.sup.+ permeability and transcellular NaCl transport in the medullary thick ascending limb of Henle's loop
format: Article
creator:
  • Himmerkus, Nina
  • Plain, Allein
  • Marques, Rita D
  • Sonntag, Svenja R
  • Paliege, Alexander
  • Leipziger, Jens
  • Bleich, Markus
subjects:
  • Permeability
  • Physiological aspects
  • Vasopressin
ispartof: Pflügers Archiv, 2017, Vol.469 (1), p.149
description: Byline: Nina Himmerkus (1), Allein Plain (1), Rita D. Marques (2), Svenja R. Sonntag (1), Alexander Paliege (3), Jens Leipziger (2), Markus Bleich (1) Keywords: Claudins; Concentration mechanism; Outer medulla; Tight junction; Sodium transport The medullary thick ascending limb of Henle's loop (mTAL) is crucial for urine-concentrating ability of the kidney. It is water tight and able to dilute the luminal fluid by active transcellular NaCl transport, fueling the counter current mechanism by increasing interstitial osmolality. While chloride is exclusively transported transcellularly, approx. 50% of sodium transport occurs via the paracellular route, driven by the lumen-positive transepithelial potential. Antidiuretic hormone (AVP) is known to increase active NaCl transport to support collecting duct water reabsorption. Here, we investigated the concomitant effects of AVP on the paracellular properties of mTAL. Freshly isolated mouse mTALs were perfused and electrophysiological transcellular and paracelluar properties were assessed in a paired fashion before and after AVP stimulation. In addition, the same parameters were measured in mice on a water-restricted (WR) or water-loaded (WL) diet for 5 days. Acute ex vivo stimulation as well as long-term in vivo water restriction increased equivalent short circuit current as a measure of active transcellular NaCl transport. Intriguingly, in both experimental approaches, this was accompanied by markedly increased paracellular Na.sup.+ selectivity. Thus, AVP is able to acutely regulate paracellular cation selectivity in parallel to transcellular NaCl transport, allowing balanced paracellular Na.sup.+ absorption under an increased transepithelial driving force. Author Affiliation: (1) Institute of Physiology, Christian-Albrechts-University, Olshausenstr. 40, 24098, Kiel, Germany (2) Department of Biomedicine, Physiology, Aarhus University, Aarhus, Denmark (3) Department of Nephrology, Charite-Universitatsmedizin Berlin, Berlin, Germany Article History: Registration Date: 23/11/2016 Received Date: 11/10/2016 Accepted Date: 22/11/2016 Online Date: 06/12/2016 Article note: Nina Himmerkus and Allein Plain contributed equally. Jens Leipziger and Markus Bleich contributed equally. This article is published as part of the Special Issue on "Physiology, pathophysiology and clinical impact of claudins."
language: eng
source:
identifier: ISSN: 0031-6768
fulltext: no_fulltext
issn:
  • 0031-6768
  • 1432-2013
url: Link


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titleAVP dynamically increases paracellular Na.sup.+ permeability and transcellular NaCl transport in the medullary thick ascending limb of Henle's loop
creatorHimmerkus, Nina ; Plain, Allein ; Marques, Rita D ; Sonntag, Svenja R ; Paliege, Alexander ; Leipziger, Jens ; Bleich, Markus
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descriptionByline: Nina Himmerkus (1), Allein Plain (1), Rita D. Marques (2), Svenja R. Sonntag (1), Alexander Paliege (3), Jens Leipziger (2), Markus Bleich (1) Keywords: Claudins; Concentration mechanism; Outer medulla; Tight junction; Sodium transport The medullary thick ascending limb of Henle's loop (mTAL) is crucial for urine-concentrating ability of the kidney. It is water tight and able to dilute the luminal fluid by active transcellular NaCl transport, fueling the counter current mechanism by increasing interstitial osmolality. While chloride is exclusively transported transcellularly, approx. 50% of sodium transport occurs via the paracellular route, driven by the lumen-positive transepithelial potential. Antidiuretic hormone (AVP) is known to increase active NaCl transport to support collecting duct water reabsorption. Here, we investigated the concomitant effects of AVP on the paracellular properties of mTAL. Freshly isolated mouse mTALs were perfused and electrophysiological transcellular and paracelluar properties were assessed in a paired fashion before and after AVP stimulation. In addition, the same parameters were measured in mice on a water-restricted (WR) or water-loaded (WL) diet for 5 days. Acute ex vivo stimulation as well as long-term in vivo water restriction increased equivalent short circuit current as a measure of active transcellular NaCl transport. Intriguingly, in both experimental approaches, this was accompanied by markedly increased paracellular Na.sup.+ selectivity. Thus, AVP is able to acutely regulate paracellular cation selectivity in parallel to transcellular NaCl transport, allowing balanced paracellular Na.sup.+ absorption under an increased transepithelial driving force. Author Affiliation: (1) Institute of Physiology, Christian-Albrechts-University, Olshausenstr. 40, 24098, Kiel, Germany (2) Department of Biomedicine, Physiology, Aarhus University, Aarhus, Denmark (3) Department of Nephrology, Charite-Universitatsmedizin Berlin, Berlin, Germany Article History: Registration Date: 23/11/2016 Received Date: 11/10/2016 Accepted Date: 22/11/2016 Online Date: 06/12/2016 Article note: Nina Himmerkus and Allein Plain contributed equally. Jens Leipziger and Markus Bleich contributed equally. This article is published as part of the Special Issue on "Physiology, pathophysiology and clinical impact of claudins."
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abstractByline: Nina Himmerkus (1), Allein Plain (1), Rita D. Marques (2), Svenja R. Sonntag (1), Alexander Paliege (3), Jens Leipziger (2), Markus Bleich (1) Keywords: Claudins; Concentration mechanism; Outer medulla; Tight junction; Sodium transport The medullary thick ascending limb of Henle's loop (mTAL) is crucial for urine-concentrating ability of the kidney. It is water tight and able to dilute the luminal fluid by active transcellular NaCl transport, fueling the counter current mechanism by increasing interstitial osmolality. While chloride is exclusively transported transcellularly, approx. 50% of sodium transport occurs via the paracellular route, driven by the lumen-positive transepithelial potential. Antidiuretic hormone (AVP) is known to increase active NaCl transport to support collecting duct water reabsorption. Here, we investigated the concomitant effects of AVP on the paracellular properties of mTAL. Freshly isolated mouse mTALs were perfused and electrophysiological transcellular and paracelluar properties were assessed in a paired fashion before and after AVP stimulation. In addition, the same parameters were measured in mice on a water-restricted (WR) or water-loaded (WL) diet for 5 days. Acute ex vivo stimulation as well as long-term in vivo water restriction increased equivalent short circuit current as a measure of active transcellular NaCl transport. Intriguingly, in both experimental approaches, this was accompanied by markedly increased paracellular Na.sup.+ selectivity. Thus, AVP is able to acutely regulate paracellular cation selectivity in parallel to transcellular NaCl transport, allowing balanced paracellular Na.sup.+ absorption under an increased transepithelial driving force. Author Affiliation: (1) Institute of Physiology, Christian-Albrechts-University, Olshausenstr. 40, 24098, Kiel, Germany (2) Department of Biomedicine, Physiology, Aarhus University, Aarhus, Denmark (3) Department of Nephrology, Charite-Universitatsmedizin Berlin, Berlin, Germany Article History: Registration Date: 23/11/2016 Received Date: 11/10/2016 Accepted Date: 22/11/2016 Online Date: 06/12/2016 Article note: Nina Himmerkus and Allein Plain contributed equally. Jens Leipziger and Markus Bleich contributed equally. This article is published as part of the Special Issue on "Physiology, pathophysiology and clinical impact of claudins."
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