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G-Protein-Coupled Receptor Interaction with Small GTPases

In addition to heterotrimeric G-proteins, Ras-like small GTPases are also involved in regulating physiological functions of the G-protein-coupled receptor (GPCR) superfamily. In particular, Rab and ARF GTPases function either as “traffic cops” to coordinate receptor targeting to specific locations o... Full description

Journal Title: Methods in Enzymology 2013, Vol.522, p.97-108
Main Author: Dong, Chunmin
Other Authors: Wu, Guangyu
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Gale eBooks
Publisher: United States: Elsevier Science & Technology
ID: ISSN: 0076-6879
Link: https://www.ncbi.nlm.nih.gov/pubmed/23374182
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title: G-Protein-Coupled Receptor Interaction with Small GTPases
format: Article
creator:
  • Dong, Chunmin
  • Wu, Guangyu
subjects:
  • ADP-Ribosylation Factor 1 - genetics
  • ADP-Ribosylation Factor 1 - metabolism
  • ARF1
  • Article
  • Binding Sites
  • G proteins
  • G-protein-coupled receptor
  • Gene Expression Regulation
  • Goods and services tax
  • HEK293 Cells
  • Humans
  • Immunoprecipitation - methods
  • Protein Binding
  • Protein Interaction Domains and Motifs
  • Protein Transport
  • Proteins
  • Protein–protein interaction
  • rab GTP-Binding Proteins - genetics
  • rab GTP-Binding Proteins - metabolism
  • Rab8
  • Receptors, Adrenergic, alpha-2 - genetics
  • Receptors, Adrenergic, alpha-2 - metabolism
  • Receptors, Adrenergic, beta-2 - genetics
  • Receptors, Adrenergic, beta-2 - metabolism
  • Signal Transduction
  • Signaling
  • Small GTPases
  • Trafficking
  • α2B-Adrenergic receptor
ispartof: Methods in Enzymology, 2013, Vol.522, p.97-108
description: In addition to heterotrimeric G-proteins, Ras-like small GTPases are also involved in regulating physiological functions of the G-protein-coupled receptor (GPCR) superfamily. In particular, Rab and ARF GTPases function either as “traffic cops” to coordinate receptor targeting to specific locations or as “signal transducers” to directly mediate receptor signal propagation. As revealed in protein–protein interaction assays, GPCRs may use specific motifs to physically interact with small GTPases, providing important insights into the underlying molecular mechanisms. In this chapter, we describe coimmunoprecipitation and GST fusion protein pull-down approaches to study the GPCR-small GTPase interaction, by focusing on the interaction of α2B- and β2-adrenegic receptors with the small GTPases Rab8 and ARF1.
language: eng
source: Gale eBooks
identifier: ISSN: 0076-6879
fulltext: fulltext
issn:
  • 0076-6879
  • 1557-7988
url: Link


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descriptionIn addition to heterotrimeric G-proteins, Ras-like small GTPases are also involved in regulating physiological functions of the G-protein-coupled receptor (GPCR) superfamily. In particular, Rab and ARF GTPases function either as “traffic cops” to coordinate receptor targeting to specific locations or as “signal transducers” to directly mediate receptor signal propagation. As revealed in protein–protein interaction assays, GPCRs may use specific motifs to physically interact with small GTPases, providing important insights into the underlying molecular mechanisms. In this chapter, we describe coimmunoprecipitation and GST fusion protein pull-down approaches to study the GPCR-small GTPase interaction, by focusing on the interaction of α2B- and β2-adrenegic receptors with the small GTPases Rab8 and ARF1.
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subjectADP-Ribosylation Factor 1 - genetics ; ADP-Ribosylation Factor 1 - metabolism ; ARF1 ; Article ; Binding Sites ; G proteins ; G-protein-coupled receptor ; Gene Expression Regulation ; Goods and services tax ; HEK293 Cells ; Humans ; Immunoprecipitation - methods ; Protein Binding ; Protein Interaction Domains and Motifs ; Protein Transport ; Proteins ; Protein–protein interaction ; rab GTP-Binding Proteins - genetics ; rab GTP-Binding Proteins - metabolism ; Rab8 ; Receptors, Adrenergic, alpha-2 - genetics ; Receptors, Adrenergic, alpha-2 - metabolism ; Receptors, Adrenergic, beta-2 - genetics ; Receptors, Adrenergic, beta-2 - metabolism ; Signal Transduction ; Signaling ; Small GTPases ; Trafficking ; α2B-Adrenergic receptor
ispartofMethods in Enzymology, 2013, Vol.522, p.97-108
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notesCurrent address: Department of Medicine, Section of Pulmonary Disease and Critical Care, Tulane University School of Medicine, New Orleans, USA.
abstractIn addition to heterotrimeric G-proteins, Ras-like small GTPases are also involved in regulating physiological functions of the G-protein-coupled receptor (GPCR) superfamily. In particular, Rab and ARF GTPases function either as “traffic cops” to coordinate receptor targeting to specific locations or as “signal transducers” to directly mediate receptor signal propagation. As revealed in protein–protein interaction assays, GPCRs may use specific motifs to physically interact with small GTPases, providing important insights into the underlying molecular mechanisms. In this chapter, we describe coimmunoprecipitation and GST fusion protein pull-down approaches to study the GPCR-small GTPase interaction, by focusing on the interaction of α2B- and β2-adrenegic receptors with the small GTPases Rab8 and ARF1.
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