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Kisspeptin Neurons in the Arcuate Nucleus of the Ewe Express Both Dynorphin A and Neurokinin B

Kisspeptin is a potent stimulator of GnRH secretion that has been implicated in the feedback actions of ovarian steroids. In ewes, the majority of hypothalamic kisspeptin neurons are found in the arcuate nucleus (ARC), with a smaller population located in the preoptic area. Most arcuate kisspeptin n... Full description

Journal Title: Endocrinology (Philadelphia) 2007-12, Vol.148 (12), p.5752-5760
Main Author: Goodman, Robert L
Other Authors: Lehman, Michael N , Smith, Jeremy T , Coolen, Lique M , de Oliveira, Cleusa V. R , Jafarzadehshirazi, Mohammad R , Pereira, Alda , Iqbal, Javed , Caraty, Alain , Ciofi, Philippe , Clarke, Iain J
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: Bethesda, MD: Endocrine Society
ID: ISSN: 0013-7227
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recordid: cdi_hal_primary_oai_HAL_hal_02662870v1
title: Kisspeptin Neurons in the Arcuate Nucleus of the Ewe Express Both Dynorphin A and Neurokinin B
format: Article
creator:
  • Goodman, Robert L
  • Lehman, Michael N
  • Smith, Jeremy T
  • Coolen, Lique M
  • de Oliveira, Cleusa V. R
  • Jafarzadehshirazi, Mohammad R
  • Pereira, Alda
  • Iqbal, Javed
  • Caraty, Alain
  • Ciofi, Philippe
  • Clarke, Iain J
subjects:
  • Abridged Index Medicus
  • Animals
  • Arcuate Nucleus of Hypothalamus - cytology
  • Arcuate Nucleus of Hypothalamus - metabolism
  • Biological and medical sciences
  • Dynorphins - analysis
  • Dynorphins - metabolism
  • Endocrinology and metabolism
  • Female
  • Fundamental and applied biological sciences. Psychology
  • gamma-MSH - analysis
  • gamma-MSH - metabolism
  • Human health and pathology
  • Immunohistochemistry
  • In Vitro Techniques
  • Kisspeptins
  • Life Sciences
  • Male
  • Neurokinin B - analysis
  • Neurokinin B - metabolism
  • Neurons - cytology
  • Neurons - metabolism
  • Sheep
  • Tumor Suppressor Proteins - analysis
  • Tumor Suppressor Proteins - metabolism
  • Vertebrates: endocrinology
ispartof: Endocrinology (Philadelphia), 2007-12, Vol.148 (12), p.5752-5760
description: Kisspeptin is a potent stimulator of GnRH secretion that has been implicated in the feedback actions of ovarian steroids. In ewes, the majority of hypothalamic kisspeptin neurons are found in the arcuate nucleus (ARC), with a smaller population located in the preoptic area. Most arcuate kisspeptin neurons express estrogen receptor-α, as do a set of arcuate neurons that contain both dynorphin and neurokinin B (NKB), suggesting that all three neuropeptides are colocalized in the same cells. In this study we tested this hypothesis using dual immunocytochemistry and also determined if kisspeptin neurons contain MSH or agouti-related peptide. To assess colocalization of kisspeptin and dynorphin, we used paraformaldehyde-fixed tissue from estrogen-treated ovariectomized ewes in the breeding season (n = 5). Almost all ARC, but no preoptic area, kisspeptin neurons contained dynorphin. Similarly, almost all ARC dynorphin neurons contained kisspeptin. In experiment 2 we examined colocalization of kisspeptin and NKB in picric-acid fixed tissue collected from ovary intact ewes (n = 9). Over three quarters of ARC kisspeptin neurons also expressed NKB, and a similar percentage of NKB neurons contained kisspeptin. In contrast, no kisspeptin neurons stained for MSH or agouti-related peptide. These data demonstrate that, in the ewe, a high percentage of ARC kisspeptin neurons also produce dynorphin and NKB, and we propose that a single subpopulation of ARC neurons contains all three neuropeptides. Because virtually all of these neurons express estrogen and progesterone re-ceptors, they are likely to relay the feedback effects of these steroids to GnRH neurons to regulate reproductive function.
language: eng
source:
identifier: ISSN: 0013-7227
fulltext: no_fulltext
issn:
  • 0013-7227
  • 1945-7170
url: Link


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titleKisspeptin Neurons in the Arcuate Nucleus of the Ewe Express Both Dynorphin A and Neurokinin B
creatorGoodman, Robert L ; Lehman, Michael N ; Smith, Jeremy T ; Coolen, Lique M ; de Oliveira, Cleusa V. R ; Jafarzadehshirazi, Mohammad R ; Pereira, Alda ; Iqbal, Javed ; Caraty, Alain ; Ciofi, Philippe ; Clarke, Iain J
creatorcontribGoodman, Robert L ; Lehman, Michael N ; Smith, Jeremy T ; Coolen, Lique M ; de Oliveira, Cleusa V. R ; Jafarzadehshirazi, Mohammad R ; Pereira, Alda ; Iqbal, Javed ; Caraty, Alain ; Ciofi, Philippe ; Clarke, Iain J
descriptionKisspeptin is a potent stimulator of GnRH secretion that has been implicated in the feedback actions of ovarian steroids. In ewes, the majority of hypothalamic kisspeptin neurons are found in the arcuate nucleus (ARC), with a smaller population located in the preoptic area. Most arcuate kisspeptin neurons express estrogen receptor-α, as do a set of arcuate neurons that contain both dynorphin and neurokinin B (NKB), suggesting that all three neuropeptides are colocalized in the same cells. In this study we tested this hypothesis using dual immunocytochemistry and also determined if kisspeptin neurons contain MSH or agouti-related peptide. To assess colocalization of kisspeptin and dynorphin, we used paraformaldehyde-fixed tissue from estrogen-treated ovariectomized ewes in the breeding season (n = 5). Almost all ARC, but no preoptic area, kisspeptin neurons contained dynorphin. Similarly, almost all ARC dynorphin neurons contained kisspeptin. In experiment 2 we examined colocalization of kisspeptin and NKB in picric-acid fixed tissue collected from ovary intact ewes (n = 9). Over three quarters of ARC kisspeptin neurons also expressed NKB, and a similar percentage of NKB neurons contained kisspeptin. In contrast, no kisspeptin neurons stained for MSH or agouti-related peptide. These data demonstrate that, in the ewe, a high percentage of ARC kisspeptin neurons also produce dynorphin and NKB, and we propose that a single subpopulation of ARC neurons contains all three neuropeptides. Because virtually all of these neurons express estrogen and progesterone re-ceptors, they are likely to relay the feedback effects of these steroids to GnRH neurons to regulate reproductive function.
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publisherBethesda, MD: Endocrine Society
subjectAbridged Index Medicus ; Animals ; Arcuate Nucleus of Hypothalamus - cytology ; Arcuate Nucleus of Hypothalamus - metabolism ; Biological and medical sciences ; Dynorphins - analysis ; Dynorphins - metabolism ; Endocrinology and metabolism ; Female ; Fundamental and applied biological sciences. Psychology ; gamma-MSH - analysis ; gamma-MSH - metabolism ; Human health and pathology ; Immunohistochemistry ; In Vitro Techniques ; Kisspeptins ; Life Sciences ; Male ; Neurokinin B - analysis ; Neurokinin B - metabolism ; Neurons - cytology ; Neurons - metabolism ; Sheep ; Tumor Suppressor Proteins - analysis ; Tumor Suppressor Proteins - metabolism ; Vertebrates: endocrinology
ispartofEndocrinology (Philadelphia), 2007-12, Vol.148 (12), p.5752-5760
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1Lehman, Michael N
2Smith, Jeremy T
3Coolen, Lique M
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5Jafarzadehshirazi, Mohammad R
6Pereira, Alda
7Iqbal, Javed
8Caraty, Alain
9Ciofi, Philippe
10Clarke, Iain J
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0Kisspeptin Neurons in the Arcuate Nucleus of the Ewe Express Both Dynorphin A and Neurokinin B
1Endocrinology (Philadelphia)
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descriptionKisspeptin is a potent stimulator of GnRH secretion that has been implicated in the feedback actions of ovarian steroids. In ewes, the majority of hypothalamic kisspeptin neurons are found in the arcuate nucleus (ARC), with a smaller population located in the preoptic area. Most arcuate kisspeptin neurons express estrogen receptor-α, as do a set of arcuate neurons that contain both dynorphin and neurokinin B (NKB), suggesting that all three neuropeptides are colocalized in the same cells. In this study we tested this hypothesis using dual immunocytochemistry and also determined if kisspeptin neurons contain MSH or agouti-related peptide. To assess colocalization of kisspeptin and dynorphin, we used paraformaldehyde-fixed tissue from estrogen-treated ovariectomized ewes in the breeding season (n = 5). Almost all ARC, but no preoptic area, kisspeptin neurons contained dynorphin. Similarly, almost all ARC dynorphin neurons contained kisspeptin. In experiment 2 we examined colocalization of kisspeptin and NKB in picric-acid fixed tissue collected from ovary intact ewes (n = 9). Over three quarters of ARC kisspeptin neurons also expressed NKB, and a similar percentage of NKB neurons contained kisspeptin. In contrast, no kisspeptin neurons stained for MSH or agouti-related peptide. These data demonstrate that, in the ewe, a high percentage of ARC kisspeptin neurons also produce dynorphin and NKB, and we propose that a single subpopulation of ARC neurons contains all three neuropeptides. Because virtually all of these neurons express estrogen and progesterone re-ceptors, they are likely to relay the feedback effects of these steroids to GnRH neurons to regulate reproductive function.
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0Abridged Index Medicus
1Animals
2Arcuate Nucleus of Hypothalamus - cytology
3Arcuate Nucleus of Hypothalamus - metabolism
4Biological and medical sciences
5Dynorphins - analysis
6Dynorphins - metabolism
7Endocrinology and metabolism
8Female
9Fundamental and applied biological sciences. Psychology
10gamma-MSH - analysis
11gamma-MSH - metabolism
12Human health and pathology
13Immunohistochemistry
14In Vitro Techniques
15Kisspeptins
16Life Sciences
17Male
18Neurokinin B - analysis
19Neurokinin B - metabolism
20Neurons - cytology
21Neurons - metabolism
22Sheep
23Tumor Suppressor Proteins - analysis
24Tumor Suppressor Proteins - metabolism
25Vertebrates: endocrinology
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titleKisspeptin Neurons in the Arcuate Nucleus of the Ewe Express Both Dynorphin A and Neurokinin B
authorGoodman, Robert L ; Lehman, Michael N ; Smith, Jeremy T ; Coolen, Lique M ; de Oliveira, Cleusa V. R ; Jafarzadehshirazi, Mohammad R ; Pereira, Alda ; Iqbal, Javed ; Caraty, Alain ; Ciofi, Philippe ; Clarke, Iain J
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0Abridged Index Medicus
1Animals
2Arcuate Nucleus of Hypothalamus - cytology
3Arcuate Nucleus of Hypothalamus - metabolism
4Biological and medical sciences
5Dynorphins - analysis
6Dynorphins - metabolism
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9Fundamental and applied biological sciences. Psychology
10gamma-MSH - analysis
11gamma-MSH - metabolism
12Human health and pathology
13Immunohistochemistry
14In Vitro Techniques
15Kisspeptins
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17Male
18Neurokinin B - analysis
19Neurokinin B - metabolism
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21Neurons - metabolism
22Sheep
23Tumor Suppressor Proteins - analysis
24Tumor Suppressor Proteins - metabolism
25Vertebrates: endocrinology
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abstractKisspeptin is a potent stimulator of GnRH secretion that has been implicated in the feedback actions of ovarian steroids. In ewes, the majority of hypothalamic kisspeptin neurons are found in the arcuate nucleus (ARC), with a smaller population located in the preoptic area. Most arcuate kisspeptin neurons express estrogen receptor-α, as do a set of arcuate neurons that contain both dynorphin and neurokinin B (NKB), suggesting that all three neuropeptides are colocalized in the same cells. In this study we tested this hypothesis using dual immunocytochemistry and also determined if kisspeptin neurons contain MSH or agouti-related peptide. To assess colocalization of kisspeptin and dynorphin, we used paraformaldehyde-fixed tissue from estrogen-treated ovariectomized ewes in the breeding season (n = 5). Almost all ARC, but no preoptic area, kisspeptin neurons contained dynorphin. Similarly, almost all ARC dynorphin neurons contained kisspeptin. In experiment 2 we examined colocalization of kisspeptin and NKB in picric-acid fixed tissue collected from ovary intact ewes (n = 9). Over three quarters of ARC kisspeptin neurons also expressed NKB, and a similar percentage of NKB neurons contained kisspeptin. In contrast, no kisspeptin neurons stained for MSH or agouti-related peptide. These data demonstrate that, in the ewe, a high percentage of ARC kisspeptin neurons also produce dynorphin and NKB, and we propose that a single subpopulation of ARC neurons contains all three neuropeptides. Because virtually all of these neurons express estrogen and progesterone re-ceptors, they are likely to relay the feedback effects of these steroids to GnRH neurons to regulate reproductive function.
copBethesda, MD
pubEndocrine Society
pmid17823266
doi10.1210/en.2007-0961
oafree_for_read