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Chitosan coating of copper nanoparticles reduces in vitro toxicity and increases inflammation in the lung

Despite their potential for a variety of applications, copper nanoparticles induce very strong inflammatory responses and cellular toxicity following aerosolized delivery. Coating metallic nanoparticles with polysaccharides, such as biocompatible and antimicrobial chitosan, has the potential to redu... Full description

Journal Title: Nanotechnology 2013, Vol.24 (39), p.395101-1-10
Main Author: Worthington, Kristan L S
Other Authors: Adamcakova-Dodd, Andrea , Wongrakpanich, Amaraporn , Mudunkotuwa, Imali A , Mapuskar, Kranti A , Joshi, Vijaya B , Allan Guymon, C , Spitz, Douglas R , Grassian, Vicki H , Thorne, Peter S , Salem, Aliasger K
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Alma/SFX Local Collection
Publisher: Bristol: IOP Publishing
ID: ISSN: 0957-4484
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recordid: cdi_iop_journals_10_1088_0957_4484_24_39_395101
title: Chitosan coating of copper nanoparticles reduces in vitro toxicity and increases inflammation in the lung
format: Article
creator:
  • Worthington, Kristan L S
  • Adamcakova-Dodd, Andrea
  • Wongrakpanich, Amaraporn
  • Mudunkotuwa, Imali A
  • Mapuskar, Kranti A
  • Joshi, Vijaya B
  • Allan Guymon, C
  • Spitz, Douglas R
  • Grassian, Vicki H
  • Thorne, Peter S
  • Salem, Aliasger K
subjects:
  • Administration, Intranasal
  • Animals
  • Article
  • Bronchoalveolar Lavage Fluid - chemistry
  • Cell Line
  • Cell Survival - drug effects
  • Chitosan
  • Chitosan - administration & dosage
  • Chitosan - pharmacology
  • Coatings
  • Copper
  • Copper - administration & dosage
  • Copper - toxicity
  • Copper Nanoparticles
  • Cross-disciplinary physics: materials science
  • rheology
  • Cytokines
  • Exact sciences and technology
  • Humans
  • Lung - chemistry
  • Lung Inflammation
  • Male
  • Materials science
  • Metal Nanoparticles - administration & dosage
  • Metal Nanoparticles - chemistry
  • Metal Nanoparticles - toxicity
  • Metal Oxide Nanoparticles
  • Mice
  • Mice, Inbred C57BL
  • Nanocrystalline materials
  • Nanoparticle Toxicity
  • Nanoscale materials and structures: fabrication and characterization
  • Physics
  • Pneumonia - chemically induced
ispartof: Nanotechnology, 2013, Vol.24 (39), p.395101-1-10
description: Despite their potential for a variety of applications, copper nanoparticles induce very strong inflammatory responses and cellular toxicity following aerosolized delivery. Coating metallic nanoparticles with polysaccharides, such as biocompatible and antimicrobial chitosan, has the potential to reduce this toxicity. In this study, copper nanoparticles were coated with chitosan using a newly developed and facile method. The presence of coating was confirmed using x-ray photoelectron spectroscopy, rhodamine tagging of chitosan followed by confocal fluorescence imaging of coated particles and observed increases in particle size and zeta potential. Further physical and chemical characteristics were evaluated using dissolution and x-ray diffraction studies. The chitosan coating was shown to significantly reduce the toxicity of copper nanoparticles after 24 and 52 h and the generation of reactive oxygen species as assayed by DHE oxidation after 24 h in vitro. Conversely, inflammatory response, measured using the number of white blood cells, total protein, and cytokines/chemokines in the bronchoalveolar fluid of mice exposed to chitosan coated versus uncoated copper nanoparticles, was shown to increase, as was the concentration of copper ions. These results suggest that coating metal nanoparticles with mucoadhesive polysaccharides (e.g. chitosan) could increase their potential for use in controlled release of copper ions to cells, but will result in a higher inflammatory response if administered via the lung.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0957-4484
fulltext: fulltext
issn:
  • 0957-4484
  • 1361-6528
url: Link


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titleChitosan coating of copper nanoparticles reduces in vitro toxicity and increases inflammation in the lung
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creatorWorthington, Kristan L S ; Adamcakova-Dodd, Andrea ; Wongrakpanich, Amaraporn ; Mudunkotuwa, Imali A ; Mapuskar, Kranti A ; Joshi, Vijaya B ; Allan Guymon, C ; Spitz, Douglas R ; Grassian, Vicki H ; Thorne, Peter S ; Salem, Aliasger K
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descriptionDespite their potential for a variety of applications, copper nanoparticles induce very strong inflammatory responses and cellular toxicity following aerosolized delivery. Coating metallic nanoparticles with polysaccharides, such as biocompatible and antimicrobial chitosan, has the potential to reduce this toxicity. In this study, copper nanoparticles were coated with chitosan using a newly developed and facile method. The presence of coating was confirmed using x-ray photoelectron spectroscopy, rhodamine tagging of chitosan followed by confocal fluorescence imaging of coated particles and observed increases in particle size and zeta potential. Further physical and chemical characteristics were evaluated using dissolution and x-ray diffraction studies. The chitosan coating was shown to significantly reduce the toxicity of copper nanoparticles after 24 and 52 h and the generation of reactive oxygen species as assayed by DHE oxidation after 24 h in vitro. Conversely, inflammatory response, measured using the number of white blood cells, total protein, and cytokines/chemokines in the bronchoalveolar fluid of mice exposed to chitosan coated versus uncoated copper nanoparticles, was shown to increase, as was the concentration of copper ions. These results suggest that coating metal nanoparticles with mucoadhesive polysaccharides (e.g. chitosan) could increase their potential for use in controlled release of copper ions to cells, but will result in a higher inflammatory response if administered via the lung.
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subjectAdministration, Intranasal ; Animals ; Article ; Bronchoalveolar Lavage Fluid - chemistry ; Cell Line ; Cell Survival - drug effects ; Chitosan ; Chitosan - administration & dosage ; Chitosan - pharmacology ; Coatings ; Copper ; Copper - administration & dosage ; Copper - toxicity ; Copper Nanoparticles ; Cross-disciplinary physics: materials science; rheology ; Cytokines ; Exact sciences and technology ; Humans ; Lung - chemistry ; Lung Inflammation ; Male ; Materials science ; Metal Nanoparticles - administration & dosage ; Metal Nanoparticles - chemistry ; Metal Nanoparticles - toxicity ; Metal Oxide Nanoparticles ; Mice ; Mice, Inbred C57BL ; Nanocrystalline materials ; Nanoparticle Toxicity ; Nanoscale materials and structures: fabrication and characterization ; Physics ; Pneumonia - chemically induced
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5Joshi, Vijaya B
6Allan Guymon, C
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descriptionDespite their potential for a variety of applications, copper nanoparticles induce very strong inflammatory responses and cellular toxicity following aerosolized delivery. Coating metallic nanoparticles with polysaccharides, such as biocompatible and antimicrobial chitosan, has the potential to reduce this toxicity. In this study, copper nanoparticles were coated with chitosan using a newly developed and facile method. The presence of coating was confirmed using x-ray photoelectron spectroscopy, rhodamine tagging of chitosan followed by confocal fluorescence imaging of coated particles and observed increases in particle size and zeta potential. Further physical and chemical characteristics were evaluated using dissolution and x-ray diffraction studies. The chitosan coating was shown to significantly reduce the toxicity of copper nanoparticles after 24 and 52 h and the generation of reactive oxygen species as assayed by DHE oxidation after 24 h in vitro. Conversely, inflammatory response, measured using the number of white blood cells, total protein, and cytokines/chemokines in the bronchoalveolar fluid of mice exposed to chitosan coated versus uncoated copper nanoparticles, was shown to increase, as was the concentration of copper ions. These results suggest that coating metal nanoparticles with mucoadhesive polysaccharides (e.g. chitosan) could increase their potential for use in controlled release of copper ions to cells, but will result in a higher inflammatory response if administered via the lung.
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4Cell Line
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6Chitosan
7Chitosan - administration & dosage
8Chitosan - pharmacology
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11Copper - administration & dosage
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13Copper Nanoparticles
14Cross-disciplinary physics: materials science; rheology
15Cytokines
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17Humans
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20Male
21Materials science
22Metal Nanoparticles - administration & dosage
23Metal Nanoparticles - chemistry
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25Metal Oxide Nanoparticles
26Mice
27Mice, Inbred C57BL
28Nanocrystalline materials
29Nanoparticle Toxicity
30Nanoscale materials and structures: fabrication and characterization
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32Pneumonia - chemically induced
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titleChitosan coating of copper nanoparticles reduces in vitro toxicity and increases inflammation in the lung
authorWorthington, Kristan L S ; Adamcakova-Dodd, Andrea ; Wongrakpanich, Amaraporn ; Mudunkotuwa, Imali A ; Mapuskar, Kranti A ; Joshi, Vijaya B ; Allan Guymon, C ; Spitz, Douglas R ; Grassian, Vicki H ; Thorne, Peter S ; Salem, Aliasger K
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8Chitosan - pharmacology
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abstractDespite their potential for a variety of applications, copper nanoparticles induce very strong inflammatory responses and cellular toxicity following aerosolized delivery. Coating metallic nanoparticles with polysaccharides, such as biocompatible and antimicrobial chitosan, has the potential to reduce this toxicity. In this study, copper nanoparticles were coated with chitosan using a newly developed and facile method. The presence of coating was confirmed using x-ray photoelectron spectroscopy, rhodamine tagging of chitosan followed by confocal fluorescence imaging of coated particles and observed increases in particle size and zeta potential. Further physical and chemical characteristics were evaluated using dissolution and x-ray diffraction studies. The chitosan coating was shown to significantly reduce the toxicity of copper nanoparticles after 24 and 52 h and the generation of reactive oxygen species as assayed by DHE oxidation after 24 h in vitro. Conversely, inflammatory response, measured using the number of white blood cells, total protein, and cytokines/chemokines in the bronchoalveolar fluid of mice exposed to chitosan coated versus uncoated copper nanoparticles, was shown to increase, as was the concentration of copper ions. These results suggest that coating metal nanoparticles with mucoadhesive polysaccharides (e.g. chitosan) could increase their potential for use in controlled release of copper ions to cells, but will result in a higher inflammatory response if administered via the lung.
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