schliessen

Filtern

 

Bibliotheken

Phosphorylation of Neurogenin2 Specifies the Migration Properties and the Dendritic Morphology of Pyramidal Neurons in the Neocortex

The molecular mechanisms specifying the dendritic morphology of different neuronal subtypes are poorly understood. Here we demonstrate that the bHLH transcription factor Neurogenin2 (Ngn2) is both necessary and sufficient for specifying the dendritic morphology of pyramidal neurons in vivo by specif... Full description

Journal Title: Neuron 2005-10-06, Vol.48 (1), p.45-62
Main Author: Hand, Randal
Other Authors: Bortone, Dante , Mattar, Pierre , Nguyen, Laurent , Heng, Julian Ik-Tsen , Guerrier, Sabrice , Boutt, Elizabeth , Peters, Eldon , Barnes, Anthony P , Parras, Carlos , Schuurmans, Carol , Guillemot, François , Polleux, Franck
Format: Electronic Article Electronic Article
Language: English
Subjects:
DNA
Quelle: Alma/SFX Local Collection
Publisher: United States: Elsevier Inc
ID: ISSN: 0896-6273
Link: https://www.ncbi.nlm.nih.gov/pubmed/16202708
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: cdi_liege_orbi_v2_oai_orbi_ulg_ac_be_2268_4937
title: Phosphorylation of Neurogenin2 Specifies the Migration Properties and the Dendritic Morphology of Pyramidal Neurons in the Neocortex
format: Article
creator:
  • Hand, Randal
  • Bortone, Dante
  • Mattar, Pierre
  • Nguyen, Laurent
  • Heng, Julian Ik-Tsen
  • Guerrier, Sabrice
  • Boutt, Elizabeth
  • Peters, Eldon
  • Barnes, Anthony P
  • Parras, Carlos
  • Schuurmans, Carol
  • Guillemot, François
  • Polleux, Franck
subjects:
  • Acquisitions & mergers
  • Age Factors
  • Anatomie (cytologie, histologie, embryologie...) & physiologie
  • Anatomy (cytology, histology, embryology...) & physiology
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors - metabolism
  • Biochemistry, biophysics & molecular biology
  • Biochimie, biophysique & biologie moléculaire
  • Blotting, Western - methods
  • Cell Count - methods
  • Cell migration
  • Cell Movement - physiology
  • Cells, Cultured
  • Chickens
  • Cloning, Molecular - methods
  • Dendrites - physiology
  • Deoxyribonucleic acid
  • DNA
  • Electrophoresis, Gel, Pulsed-Field - methods
  • Electroporation - methods
  • Embryo, Mammalian
  • Embryo, Nonmammalian
  • Female
  • Fluorescent Antibody Technique - methods
  • Gene Expression Regulation, Developmental - physiology
  • Green Fluorescent Proteins - metabolism
  • Humans
  • In Vitro Techniques
  • Leukocyte migration
  • Life sciences
  • Male
  • Mice
  • Microscopy, Confocal - methods
  • Microtubule-Associated Proteins - metabolism
  • Migration
  • Models, Biological
  • Molecular modelling
  • Morphology
  • Neocortex
  • Neocortex - cytology
  • Neocortex - embryology
  • Neocortex - metabolism
  • Neocortex/cytology/embryology/metabolism
  • Nerve Tissue Proteins - metabolism
  • Neural stem cells
  • Neurons
  • Neurophysiology
  • Neuroscience(all)
  • Neurosciences
  • Phosphorylation
  • Polarity
  • Pregnancy
  • Proteins
  • Pyramidal cells
  • Pyramidal Cells - cytology
  • Pyramidal Cells - physiology
  • Pyramidal Cells/cytology/physiology
  • rhoA GTP-Binding Protein - metabolism
  • RhoA protein
  • Sciences du vivant
  • Sequence Alignment
  • Stem Cells - physiology
  • Time Factors
  • Transcription factors
  • Tubulin - metabolism
  • Tyrosine
  • Tyrosine - metabolism
ispartof: Neuron, 2005-10-06, Vol.48 (1), p.45-62
description: The molecular mechanisms specifying the dendritic morphology of different neuronal subtypes are poorly understood. Here we demonstrate that the bHLH transcription factor Neurogenin2 (Ngn2) is both necessary and sufficient for specifying the dendritic morphology of pyramidal neurons in vivo by specifying the polarity of its leading process during the initiation of radial migration. The ability of Ngn2 to promote a polarized leading process outgrowth requires the phosphorylation of a single tyrosine residue at position 241, an event that is neither involved in Ngn2 direct transactivation properties nor its proneural function. Interestingly, the migration defect observed in the Ngn2 knockout mouse and in progenitors expressing the Ngn2Y241F mutation can be rescued by inhibiting the activity of the small-GTPase RhoA in cortical progenitors. Our results demonstrate that Ngn2 coordinates the acquisition of the radial migration properties and the unipolar dendritic morphology characterizing pyramidal neurons through molecular mechanisms distinct from those mediating its proneural activity.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0896-6273
fulltext: fulltext
issn:
  • 0896-6273
  • 1097-4199
  • 1097-4199
url: Link


@attributes
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
RANK2.5734177
LOCALfalse
PrimoNMBib
record
control
sourceidgale_opena
recordidTN_cdi_liege_orbi_v2_oai_orbi_ulg_ac_be_2268_4937
sourceformatXML
sourcesystemPC
galeidA665540992
sourcerecordidA665540992
originalsourceidFETCH-LOGICAL-1652t-ffedec641dc5c8bbb6d7d464f3546657144dc901b7f1402c6231011efe62d68b3
addsrcrecordideNqNkl-P1CAUxRujcdfVb2BMExPfpgKlUHww2az_NtldJ1GfCYVLh0kHZqGzcd794NLpRJN9cE0fKHDOj8vlFMVLjCqMMHu7rjzsYvAVQaipUFuhmjwqTjESfEGxEI-LU9QKtmCE1yfFs5TWCGHaCPy0OMGMIMJRe1r8Wq5C2q5C3A9qdMGXwZY3E7cH7zwpv21BO-sgleMKymvXx1m2jGELcZw2lDeHzQ_gTXSj0-V1iBk5hH4_4Zb7qDbOqGEG-1Q6fzDcQNAhjvDzefHEqiHBi-N4Vvz49PH7xZfF1dfPlxfnVwvMGjIurAUDmlFsdKPbruuY4YYyauuGMtZwTKnRAuGOW0wR0YzUuVMYLDBiWNvVZ8XlzM21e-UiyG10GxX3MignjYdRdipBnskaIaK47cBqQ1uWj-hEPs0ACMUwqMzCM2tw0GdL7Jy8IwfQ4X839FJp2YEkhLWSippnz5vZs43hdgdplBuXNAyD8hB2SbKWMcR4_aAQC4Ypr1kWvr4nXIdd9LmJEjeoZk2D2glXzapeDSCdt2GMSufPwMbp4MG6vH6ee9hQJAT5X0Mug_N8PZoNdDboGFKKYP90FiM5xVWu5RxXOcVVolbmuGbbq2P5u24D5q_pmM8seHePq914SGAuyA0P0d_PZsiZunMQZdIOvAaTn16P0gT3b8Bv8xESPg
sourcetypeOpen Access Repository
isCDItrue
recordtypearticle
pqid1503655083
display
typearticle
titlePhosphorylation of Neurogenin2 Specifies the Migration Properties and the Dendritic Morphology of Pyramidal Neurons in the Neocortex
sourceAlma/SFX Local Collection
creatorHand, Randal ; Bortone, Dante ; Mattar, Pierre ; Nguyen, Laurent ; Heng, Julian Ik-Tsen ; Guerrier, Sabrice ; Boutt, Elizabeth ; Peters, Eldon ; Barnes, Anthony P ; Parras, Carlos ; Schuurmans, Carol ; Guillemot, François ; Polleux, Franck
creatorcontribHand, Randal ; Bortone, Dante ; Mattar, Pierre ; Nguyen, Laurent ; Heng, Julian Ik-Tsen ; Guerrier, Sabrice ; Boutt, Elizabeth ; Peters, Eldon ; Barnes, Anthony P ; Parras, Carlos ; Schuurmans, Carol ; Guillemot, François ; Polleux, Franck
descriptionThe molecular mechanisms specifying the dendritic morphology of different neuronal subtypes are poorly understood. Here we demonstrate that the bHLH transcription factor Neurogenin2 (Ngn2) is both necessary and sufficient for specifying the dendritic morphology of pyramidal neurons in vivo by specifying the polarity of its leading process during the initiation of radial migration. The ability of Ngn2 to promote a polarized leading process outgrowth requires the phosphorylation of a single tyrosine residue at position 241, an event that is neither involved in Ngn2 direct transactivation properties nor its proneural function. Interestingly, the migration defect observed in the Ngn2 knockout mouse and in progenitors expressing the Ngn2Y241F mutation can be rescued by inhibiting the activity of the small-GTPase RhoA in cortical progenitors. Our results demonstrate that Ngn2 coordinates the acquisition of the radial migration properties and the unipolar dendritic morphology characterizing pyramidal neurons through molecular mechanisms distinct from those mediating its proneural activity.
identifier
0ISSN: 0896-6273
1ISSN: 1097-4199
2EISSN: 1097-4199
3DOI: 10.1016/j.neuron.2005.08.032
4PMID: 16202708
languageeng
publisherUnited States: Elsevier Inc
subjectAcquisitions & mergers ; Age Factors ; Anatomie (cytologie, histologie, embryologie...) & physiologie ; Anatomy (cytology, histology, embryology...) & physiology ; Animals ; Basic Helix-Loop-Helix Transcription Factors - metabolism ; Biochemistry, biophysics & molecular biology ; Biochimie, biophysique & biologie moléculaire ; Blotting, Western - methods ; Cell Count - methods ; Cell migration ; Cell Movement - physiology ; Cells, Cultured ; Chickens ; Cloning, Molecular - methods ; Dendrites - physiology ; Deoxyribonucleic acid ; DNA ; Electrophoresis, Gel, Pulsed-Field - methods ; Electroporation - methods ; Embryo, Mammalian ; Embryo, Nonmammalian ; Female ; Fluorescent Antibody Technique - methods ; Gene Expression Regulation, Developmental - physiology ; Green Fluorescent Proteins - metabolism ; Humans ; In Vitro Techniques ; Leukocyte migration ; Life sciences ; Male ; Mice ; Microscopy, Confocal - methods ; Microtubule-Associated Proteins - metabolism ; Migration ; Models, Biological ; Molecular modelling ; Morphology ; Neocortex ; Neocortex - cytology ; Neocortex - embryology ; Neocortex - metabolism ; Neocortex/cytology/embryology/metabolism ; Nerve Tissue Proteins - metabolism ; Neural stem cells ; Neurons ; Neurophysiology ; Neuroscience(all) ; Neurosciences ; Phosphorylation ; Polarity ; Pregnancy ; Proteins ; Pyramidal cells ; Pyramidal Cells - cytology ; Pyramidal Cells - physiology ; Pyramidal Cells/cytology/physiology ; rhoA GTP-Binding Protein - metabolism ; RhoA protein ; Sciences du vivant ; Sequence Alignment ; Stem Cells - physiology ; Time Factors ; Transcription factors ; Tubulin - metabolism ; Tyrosine ; Tyrosine - metabolism
ispartofNeuron, 2005-10-06, Vol.48 (1), p.45-62
rights
02005 Elsevier Inc.
1COPYRIGHT 2005 Elsevier B.V.
22005. Elsevier Inc.
lds50peer_reviewed
oafree_for_read
citedbyFETCH-LOGICAL-1652t-ffedec641dc5c8bbb6d7d464f3546657144dc901b7f1402c6231011efe62d68b3
citesFETCH-LOGICAL-1652t-ffedec641dc5c8bbb6d7d464f3546657144dc901b7f1402c6231011efe62d68b3
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
thumbnail$$Usyndetics_thumb_exl
backlink$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16202708$$D View this record in MEDLINE/PubMed
search
creatorcontrib
0Hand, Randal
1Bortone, Dante
2Mattar, Pierre
3Nguyen, Laurent
4Heng, Julian Ik-Tsen
5Guerrier, Sabrice
6Boutt, Elizabeth
7Peters, Eldon
8Barnes, Anthony P
9Parras, Carlos
10Schuurmans, Carol
11Guillemot, François
12Polleux, Franck
title
0Phosphorylation of Neurogenin2 Specifies the Migration Properties and the Dendritic Morphology of Pyramidal Neurons in the Neocortex
1Neuron
addtitleNeuron
descriptionThe molecular mechanisms specifying the dendritic morphology of different neuronal subtypes are poorly understood. Here we demonstrate that the bHLH transcription factor Neurogenin2 (Ngn2) is both necessary and sufficient for specifying the dendritic morphology of pyramidal neurons in vivo by specifying the polarity of its leading process during the initiation of radial migration. The ability of Ngn2 to promote a polarized leading process outgrowth requires the phosphorylation of a single tyrosine residue at position 241, an event that is neither involved in Ngn2 direct transactivation properties nor its proneural function. Interestingly, the migration defect observed in the Ngn2 knockout mouse and in progenitors expressing the Ngn2Y241F mutation can be rescued by inhibiting the activity of the small-GTPase RhoA in cortical progenitors. Our results demonstrate that Ngn2 coordinates the acquisition of the radial migration properties and the unipolar dendritic morphology characterizing pyramidal neurons through molecular mechanisms distinct from those mediating its proneural activity.
subject
0Acquisitions & mergers
1Age Factors
2Anatomie (cytologie, histologie, embryologie...) & physiologie
3Anatomy (cytology, histology, embryology...) & physiology
4Animals
5Basic Helix-Loop-Helix Transcription Factors - metabolism
6Biochemistry, biophysics & molecular biology
7Biochimie, biophysique & biologie moléculaire
8Blotting, Western - methods
9Cell Count - methods
10Cell migration
11Cell Movement - physiology
12Cells, Cultured
13Chickens
14Cloning, Molecular - methods
15Dendrites - physiology
16Deoxyribonucleic acid
17DNA
18Electrophoresis, Gel, Pulsed-Field - methods
19Electroporation - methods
20Embryo, Mammalian
21Embryo, Nonmammalian
22Female
23Fluorescent Antibody Technique - methods
24Gene Expression Regulation, Developmental - physiology
25Green Fluorescent Proteins - metabolism
26Humans
27In Vitro Techniques
28Leukocyte migration
29Life sciences
30Male
31Mice
32Microscopy, Confocal - methods
33Microtubule-Associated Proteins - metabolism
34Migration
35Models, Biological
36Molecular modelling
37Morphology
38Neocortex
39Neocortex - cytology
40Neocortex - embryology
41Neocortex - metabolism
42Neocortex/cytology/embryology/metabolism
43Nerve Tissue Proteins - metabolism
44Neural stem cells
45Neurons
46Neurophysiology
47Neuroscience(all)
48Neurosciences
49Phosphorylation
50Polarity
51Pregnancy
52Proteins
53Pyramidal cells
54Pyramidal Cells - cytology
55Pyramidal Cells - physiology
56Pyramidal Cells/cytology/physiology
57rhoA GTP-Binding Protein - metabolism
58RhoA protein
59Sciences du vivant
60Sequence Alignment
61Stem Cells - physiology
62Time Factors
63Transcription factors
64Tubulin - metabolism
65Tyrosine
66Tyrosine - metabolism
issn
00896-6273
11097-4199
21097-4199
fulltexttrue
rsrctypearticle
creationdate2005
recordtypearticle
recordideNqNkl-P1CAUxRujcdfVb2BMExPfpgKlUHww2az_NtldJ1GfCYVLh0kHZqGzcd794NLpRJN9cE0fKHDOj8vlFMVLjCqMMHu7rjzsYvAVQaipUFuhmjwqTjESfEGxEI-LU9QKtmCE1yfFs5TWCGHaCPy0OMGMIMJRe1r8Wq5C2q5C3A9qdMGXwZY3E7cH7zwpv21BO-sgleMKymvXx1m2jGELcZw2lDeHzQ_gTXSj0-V1iBk5hH4_4Zb7qDbOqGEG-1Q6fzDcQNAhjvDzefHEqiHBi-N4Vvz49PH7xZfF1dfPlxfnVwvMGjIurAUDmlFsdKPbruuY4YYyauuGMtZwTKnRAuGOW0wR0YzUuVMYLDBiWNvVZ8XlzM21e-UiyG10GxX3MignjYdRdipBnskaIaK47cBqQ1uWj-hEPs0ACMUwqMzCM2tw0GdL7Jy8IwfQ4X839FJp2YEkhLWSippnz5vZs43hdgdplBuXNAyD8hB2SbKWMcR4_aAQC4Ypr1kWvr4nXIdd9LmJEjeoZk2D2glXzapeDSCdt2GMSufPwMbp4MG6vH6ee9hQJAT5X0Mug_N8PZoNdDboGFKKYP90FiM5xVWu5RxXOcVVolbmuGbbq2P5u24D5q_pmM8seHePq914SGAuyA0P0d_PZsiZunMQZdIOvAaTn16P0gT3b8Bv8xESPg
startdate20051006
enddate20051006
creator
0Hand, Randal
1Bortone, Dante
2Mattar, Pierre
3Nguyen, Laurent
4Heng, Julian Ik-Tsen
5Guerrier, Sabrice
6Boutt, Elizabeth
7Peters, Eldon
8Barnes, Anthony P
9Parras, Carlos
10Schuurmans, Carol
11Guillemot, François
12Polleux, Franck
general
0Elsevier Inc
1Elsevier B.V
2Elsevier Limited
3Cell Press
4Elsevier BV
scope
06I.
1AAFTH
2CGR
3CUY
4CVF
5ECM
6EIF
7NPM
8AAYXX
9CITATION
10BSHEE
117QP
127QR
137TK
148FD
15FR3
16K9.
17P64
18RC3
197X8
20Q33
21BOBZL
22CLFQK
sort
creationdate20051006
titlePhosphorylation of Neurogenin2 Specifies the Migration Properties and the Dendritic Morphology of Pyramidal Neurons in the Neocortex
authorHand, Randal ; Bortone, Dante ; Mattar, Pierre ; Nguyen, Laurent ; Heng, Julian Ik-Tsen ; Guerrier, Sabrice ; Boutt, Elizabeth ; Peters, Eldon ; Barnes, Anthony P ; Parras, Carlos ; Schuurmans, Carol ; Guillemot, François ; Polleux, Franck
facets
frbrtype5
frbrgroupidcdi_FETCH-LOGICAL-1652t-ffedec641dc5c8bbb6d7d464f3546657144dc901b7f1402c6231011efe62d68b3
rsrctypearticles
prefilterarticles
languageeng
creationdate2005
topic
0Acquisitions & mergers
1Age Factors
2Anatomie (cytologie, histologie, embryologie...) & physiologie
3Anatomy (cytology, histology, embryology...) & physiology
4Animals
5Basic Helix-Loop-Helix Transcription Factors - metabolism
6Biochemistry, biophysics & molecular biology
7Biochimie, biophysique & biologie moléculaire
8Blotting, Western - methods
9Cell Count - methods
10Cell migration
11Cell Movement - physiology
12Cells, Cultured
13Chickens
14Cloning, Molecular - methods
15Dendrites - physiology
16Deoxyribonucleic acid
17DNA
18Electrophoresis, Gel, Pulsed-Field - methods
19Electroporation - methods
20Embryo, Mammalian
21Embryo, Nonmammalian
22Female
23Fluorescent Antibody Technique - methods
24Gene Expression Regulation, Developmental - physiology
25Green Fluorescent Proteins - metabolism
26Humans
27In Vitro Techniques
28Leukocyte migration
29Life sciences
30Male
31Mice
32Microscopy, Confocal - methods
33Microtubule-Associated Proteins - metabolism
34Migration
35Models, Biological
36Molecular modelling
37Morphology
38Neocortex
39Neocortex - cytology
40Neocortex - embryology
41Neocortex - metabolism
42Neocortex/cytology/embryology/metabolism
43Nerve Tissue Proteins - metabolism
44Neural stem cells
45Neurons
46Neurophysiology
47Neuroscience(all)
48Neurosciences
49Phosphorylation
50Polarity
51Pregnancy
52Proteins
53Pyramidal cells
54Pyramidal Cells - cytology
55Pyramidal Cells - physiology
56Pyramidal Cells/cytology/physiology
57rhoA GTP-Binding Protein - metabolism
58RhoA protein
59Sciences du vivant
60Sequence Alignment
61Stem Cells - physiology
62Time Factors
63Transcription factors
64Tubulin - metabolism
65Tyrosine
66Tyrosine - metabolism
toplevel
0peer_reviewed
1online_resources
creatorcontrib
0Hand, Randal
1Bortone, Dante
2Mattar, Pierre
3Nguyen, Laurent
4Heng, Julian Ik-Tsen
5Guerrier, Sabrice
6Boutt, Elizabeth
7Peters, Eldon
8Barnes, Anthony P
9Parras, Carlos
10Schuurmans, Carol
11Guillemot, François
12Polleux, Franck
collection
0ScienceDirect Open Access Titles
1Elsevier:ScienceDirect:Open Access
2Medline
3MEDLINE
4MEDLINE (Ovid)
5MEDLINE
6MEDLINE
7PubMed
8CrossRef
9Academic OneFile (A&I only)
10Calcium & Calcified Tissue Abstracts
11Chemoreception Abstracts
12Neurosciences Abstracts
13Technology Research Database
14Engineering Research Database
15ProQuest Health & Medical Complete (Alumni)
16Biotechnology and BioEngineering Abstracts
17Genetics Abstracts
18MEDLINE - Academic
19Université de Liège - Open Repository and Bibliography (ORBI)
20OpenAIRE (Open Access)
21OpenAIRE
jtitleNeuron
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
au
0Hand, Randal
1Bortone, Dante
2Mattar, Pierre
3Nguyen, Laurent
4Heng, Julian Ik-Tsen
5Guerrier, Sabrice
6Boutt, Elizabeth
7Peters, Eldon
8Barnes, Anthony P
9Parras, Carlos
10Schuurmans, Carol
11Guillemot, François
12Polleux, Franck
formatjournal
genrearticle
ristypeJOUR
atitlePhosphorylation of Neurogenin2 Specifies the Migration Properties and the Dendritic Morphology of Pyramidal Neurons in the Neocortex
jtitleNeuron
addtitleNeuron
date2005-10-06
risdate2005
volume48
issue1
spage45
epage62
pages45-62
issn
00896-6273
11097-4199
eissn1097-4199
notesscopus-id:25644442564
abstractThe molecular mechanisms specifying the dendritic morphology of different neuronal subtypes are poorly understood. Here we demonstrate that the bHLH transcription factor Neurogenin2 (Ngn2) is both necessary and sufficient for specifying the dendritic morphology of pyramidal neurons in vivo by specifying the polarity of its leading process during the initiation of radial migration. The ability of Ngn2 to promote a polarized leading process outgrowth requires the phosphorylation of a single tyrosine residue at position 241, an event that is neither involved in Ngn2 direct transactivation properties nor its proneural function. Interestingly, the migration defect observed in the Ngn2 knockout mouse and in progenitors expressing the Ngn2Y241F mutation can be rescued by inhibiting the activity of the small-GTPase RhoA in cortical progenitors. Our results demonstrate that Ngn2 coordinates the acquisition of the radial migration properties and the unipolar dendritic morphology characterizing pyramidal neurons through molecular mechanisms distinct from those mediating its proneural activity.
copUnited States
pubElsevier Inc
pmid16202708
doi10.1016/j.neuron.2005.08.032
oafree_for_read