MicroRNA-409-3p inhibits migration and invasion of bladder cancer cells via targeting c-Met

Journal Title:	Molecules and Cells 36(1), pp.62-68
Main Author:	Xu, X., Zhejiang University, Qingchun , China
Other Authors:	Chen, H., Zhejiang University, Qingchun , China , Lin, Y.W., Zhejiang University, Qingchun , China , Hu, Z.H., Zhejiang University, Qingchun , China , Mao, Y.Q., Zhejiang University, Qingchun , China , Wu, J., Zhejiang University, Qingchun , China , Xu, X.L., Zhejiang University, Qingchun , China , Zhu, Y., Zhejiang University, Qingchun , China , Li, S., Zhejiang University, Qingchun , China , Zheng, X.Y., Zhejiang University, Qingchun , China , Xie, L.P., Zhejiang University, Qingchun , China
Format:	Electronic Article
Language:	English
Subjects:	3' Untranslated Regions - genetics Analysis Article Articles Base Sequence Biochemistry Biomedical and Life Sciences Biomedicine Biotechnology Bladder cancer c-Met Cancer cells Cell Biology Cell Line, Tumor Cell Movement - genetics Cell Proliferation Development and progression Down-Regulation - genetics Gene Expression Regulation, Neoplastic Gene Knockdown Techniques general Humans Life Sciences Matrix Metalloproteinase 2 - genetics Matrix Metalloproteinase 2 - metabolism Matrix Metalloproteinase 9 - genetics Matrix Metalloproteinase 9 - metabolism metastasis metastasis,bladder cancer, c-Met, microRNA-409-3p MicroRNA microRNA-409-3p MicroRNAs - genetics MicroRNAs - metabolism MIGRACION MIGRATION Molecular Sequence Data Neoplasm Invasiveness Protein Binding - genetics Proteins Proto-Oncogene Proteins c-met - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism RNA, Small Interfering - metabolism Transfection Urinary Bladder Neoplasms - genetics Urinary Bladder Neoplasms - pathology 생물학
Publisher:	Dordrecht: Springer Netherlands
ID:	ISSN: 1016-8478
Zum Text:

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title:	MicroRNA-409-3p inhibits migration and invasion of bladder cancer cells via targeting c-Met
format:	Article
creator:	Xu, X., Zhejiang University, Qingchun , China Chen, H., Zhejiang University, Qingchun , China Lin, Y.W., Zhejiang University, Qingchun , China Hu, Z.H., Zhejiang University, Qingchun , China Mao, Y.Q., Zhejiang University, Qingchun , China Wu, J., Zhejiang University, Qingchun , China Xu, X.L., Zhejiang University, Qingchun , China Zhu, Y., Zhejiang University, Qingchun , China Li, S., Zhejiang University, Qingchun , China Zheng, X.Y., Zhejiang University, Qingchun , China Xie, L.P., Zhejiang University, Qingchun , China
subjects:	3' Untranslated Regions - genetics Analysis Article Articles Base Sequence Biochemistry Biomedical and Life Sciences Biomedicine Biotechnology Bladder cancer c-Met Cancer cells Cell Biology Cell Line, Tumor Cell Movement - genetics Cell Proliferation Development and progression Down-Regulation - genetics Gene Expression Regulation, Neoplastic Gene Knockdown Techniques general Humans Life Sciences Matrix Metalloproteinase 2 - genetics Matrix Metalloproteinase 2 - metabolism Matrix Metalloproteinase 9 - genetics Matrix Metalloproteinase 9 - metabolism metastasis metastasis,bladder cancer, c-Met, microRNA-409-3p MicroRNA microRNA-409-3p MicroRNAs - genetics MicroRNAs - metabolism MIGRACION MIGRATION Molecular Sequence Data Neoplasm Invasiveness Protein Binding - genetics Proteins Proto-Oncogene Proteins c-met - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism RNA, Small Interfering - metabolism Transfection Urinary Bladder Neoplasms - genetics Urinary Bladder Neoplasms - pathology 생물학
ispartof:	Molecules and Cells, 36(1), pp.62-68
description:	There is increasing evidence suggesting that dysregulation of certain microRNAs (miRNAs) may contribute to tumor progression and metastasis. Previous studies have shown that miR-409-3p is dysregulated in some malignancies, but its role in bladder cancer is still unknown. Here, we find that miR-409-3p is down-regulated in human bladder cancer tissues and cell lines. Enforced expression of miR-409-3p in bladder cancer cells significantly reduced their migration and invasion without affecting cell viability. Bioinformatics analysis identified the pro-metastatic gene c-Met as a potential miR-409-3p target. Further studies indicated that miR-409-3p suppressed the expression of c- Met by binding to its 3′-untranslated region. Silencing of c- Met by small interfering RNAs phenocopied the effects of miR-409-3p overexpression, whereas restoration of c-Met in bladder cancer cells bladder cancer cells overexpressing miR-409-3p, partially reversed the suppressive effects of miR-409-3p. We further showed that MMP2 and MMP9 may be downstream effector proteins of miR-409-3p. These findings indicate that miR-409-3p could be a potential tumor suppressor in bladder cancer.
language:	eng
source:
identifier:	ISSN: 1016-8478
fulltext:	no_fulltext
issn:	1016-8478 0219-1032
url:	Link

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MicroRNA-409-3p inhibits migration and invasion of bladder cancer cells via targeting c-Met

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Xu, X., Zhejiang University, Qingchun , China ; Chen, H., Zhejiang University, Qingchun , China ; Lin, Y.W., Zhejiang University, Qingchun , China ; Hu, Z.H., Zhejiang University, Qingchun , China ; Mao, Y.Q., Zhejiang University, Qingchun , China ; Wu, J., Zhejiang University, Qingchun , China ; Xu, X.L., Zhejiang University, Qingchun , China ; Zhu, Y., Zhejiang University, Qingchun , China ; Li, S., Zhejiang University, Qingchun , China ; Zheng, X.Y., Zhejiang University, Qingchun , China ; Xie, L.P., Zhejiang University, Qingchun , China

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description

There is increasing evidence suggesting that dysregulation of certain microRNAs (miRNAs) may contribute to tumor progression and metastasis. Previous studies have shown that miR-409-3p is dysregulated in some malignancies, but its role in bladder cancer is still unknown. Here, we find that miR-409-3p is down-regulated in human bladder cancer tissues and cell lines. Enforced expression of miR-409-3p in bladder cancer cells significantly reduced their migration and invasion without affecting cell viability. Bioinformatics analysis identified the pro-metastatic gene c-Met as a potential miR-409-3p target. Further studies indicated that miR-409-3p suppressed the expression of c- Met by binding to its 3′-untranslated region. Silencing of c- Met by small interfering RNAs phenocopied the effects of miR-409-3p overexpression, whereas restoration of c-Met in bladder cancer cells bladder cancer cells overexpressing miR-409-3p, partially reversed the suppressive effects of miR-409-3p. We further showed that MMP2 and MMP9 may be downstream effector proteins of miR-409-3p. These findings indicate that miR-409-3p could be a potential tumor suppressor in bladder cancer.

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0	ISSN: 1016-8478
1	EISSN: 0219-1032
2	DOI: 10.1007/s10059-013-0044-7
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Dordrecht: Springer Netherlands

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3' Untranslated Regions - genetics ; Analysis ; Article ; Articles ; Base Sequence ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Biotechnology ; Bladder cancer ; c-Met ; Cancer cells ; Cell Biology ; Cell Line, Tumor ; Cell Movement - genetics ; Cell Proliferation ; Development and progression ; Down-Regulation - genetics ; Gene Expression Regulation, Neoplastic ; Gene Knockdown Techniques ; general ; Humans ; Life Sciences ; Matrix Metalloproteinase 2 - genetics ; Matrix Metalloproteinase 2 - metabolism ; Matrix Metalloproteinase 9 - genetics ; Matrix Metalloproteinase 9 - metabolism ; metastasis ; metastasis,bladder cancer, c-Met, microRNA-409-3p ; MicroRNA ; microRNA-409-3p ; MicroRNAs - genetics ; MicroRNAs - metabolism ; MIGRACION ; MIGRATION ; Molecular Sequence Data ; Neoplasm Invasiveness ; Protein Binding - genetics ; Proteins ; Proto-Oncogene Proteins c-met - metabolism ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; RNA, Small Interfering - metabolism ; Transfection ; Urinary Bladder Neoplasms - genetics ; Urinary Bladder Neoplasms - pathology ; 생물학

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Molecules and Cells, 36(1), pp.62-68

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0	The Korean Society for Molecular and Cellular Biology and Springer Netherlands 2013
1	COPYRIGHT 2013 Springer
2	The Korean Society for Molecular and Cellular Biology. All rights reserved. 2013

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1	Chen, H., Zhejiang University, Qingchun , China
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3	Hu, Z.H., Zhejiang University, Qingchun , China
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8	Li, S., Zhejiang University, Qingchun , China
9	Zheng, X.Y., Zhejiang University, Qingchun , China
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creationdate	201307
title	MicroRNA-409-3p inhibits migration and invasion of bladder cancer cells via targeting c-Met
author	Xu, X., Zhejiang University, Qingchun , China ; Chen, H., Zhejiang University, Qingchun , China ; Lin, Y.W., Zhejiang University, Qingchun , China ; Hu, Z.H., Zhejiang University, Qingchun , China ; Mao, Y.Q., Zhejiang University, Qingchun , China ; Wu, J., Zhejiang University, Qingchun , China ; Xu, X.L., Zhejiang University, Qingchun , China ; Zhu, Y., Zhejiang University, Qingchun , China ; Li, S., Zhejiang University, Qingchun , China ; Zheng, X.Y., Zhejiang University, Qingchun , China ; Xie, L.P., Zhejiang University, Qingchun , China

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42	RNA, Small Interfering - metabolism
43	Transfection
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0	Xu, X., Zhejiang University, Qingchun , China
1	Chen, H., Zhejiang University, Qingchun , China
2	Lin, Y.W., Zhejiang University, Qingchun , China
3	Hu, Z.H., Zhejiang University, Qingchun , China
4	Mao, Y.Q., Zhejiang University, Qingchun , China
5	Wu, J., Zhejiang University, Qingchun , China
6	Xu, X.L., Zhejiang University, Qingchun , China
7	Zhu, Y., Zhejiang University, Qingchun , China
8	Li, S., Zhejiang University, Qingchun , China
9	Zheng, X.Y., Zhejiang University, Qingchun , China
10	Xie, L.P., Zhejiang University, Qingchun , China

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0	Xu, X., Zhejiang University, Qingchun , China
1	Chen, H., Zhejiang University, Qingchun , China
2	Lin, Y.W., Zhejiang University, Qingchun , China
3	Hu, Z.H., Zhejiang University, Qingchun , China
4	Mao, Y.Q., Zhejiang University, Qingchun , China
5	Wu, J., Zhejiang University, Qingchun , China
6	Xu, X.L., Zhejiang University, Qingchun , China
7	Zhu, Y., Zhejiang University, Qingchun , China
8	Li, S., Zhejiang University, Qingchun , China
9	Zheng, X.Y., Zhejiang University, Qingchun , China
10	Xie, L.P., Zhejiang University, Qingchun , China

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MicroRNA-409-3p inhibits migration and invasion of bladder cancer cells via targeting c-Met

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Molecules and cells

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2013

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Dordrecht

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Springer Netherlands

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23820886

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10.1007/s10059-013-0044-7

free_for_read

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MicroRNA-409-3p inhibits migration and invasion of bladder cancer cells via targeting c-Met