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MicroRNA-409-3p inhibits migration and invasion of bladder cancer cells via targeting c-Met

There is increasing evidence suggesting that dysregulation of certain microRNAs (miRNAs) may contribute to tumor progression and metastasis. Previous studies have shown that miR-409-3p is dysregulated in some malignancies, but its role in bladder cancer is still unknown. Here, we find that miR-409-3... Full description

Journal Title: Molecules and Cells 36(1), pp.62-68
Main Author: Xu, X., Zhejiang University, Qingchun , China
Other Authors: Chen, H., Zhejiang University, Qingchun , China , Lin, Y.W., Zhejiang University, Qingchun , China , Hu, Z.H., Zhejiang University, Qingchun , China , Mao, Y.Q., Zhejiang University, Qingchun , China , Wu, J., Zhejiang University, Qingchun , China , Xu, X.L., Zhejiang University, Qingchun , China , Zhu, Y., Zhejiang University, Qingchun , China , Li, S., Zhejiang University, Qingchun , China , Zheng, X.Y., Zhejiang University, Qingchun , China , Xie, L.P., Zhejiang University, Qingchun , China
Format: Electronic Article Electronic Article
Language: English
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Publisher: Dordrecht: Springer Netherlands
ID: ISSN: 1016-8478
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recordid: cdi_nrf_kci_oai_kci_go_kr_ARTI_71630
title: MicroRNA-409-3p inhibits migration and invasion of bladder cancer cells via targeting c-Met
format: Article
creator:
  • Xu, X., Zhejiang University, Qingchun , China
  • Chen, H., Zhejiang University, Qingchun , China
  • Lin, Y.W., Zhejiang University, Qingchun , China
  • Hu, Z.H., Zhejiang University, Qingchun , China
  • Mao, Y.Q., Zhejiang University, Qingchun , China
  • Wu, J., Zhejiang University, Qingchun , China
  • Xu, X.L., Zhejiang University, Qingchun , China
  • Zhu, Y., Zhejiang University, Qingchun , China
  • Li, S., Zhejiang University, Qingchun , China
  • Zheng, X.Y., Zhejiang University, Qingchun , China
  • Xie, L.P., Zhejiang University, Qingchun , China
subjects:
  • 3' Untranslated Regions - genetics
  • Analysis
  • Article
  • Articles
  • Base Sequence
  • Biochemistry
  • Biomedical and Life Sciences
  • Biomedicine
  • Biotechnology
  • Bladder cancer
  • c-Met
  • Cancer cells
  • Cell Biology
  • Cell Line, Tumor
  • Cell Movement - genetics
  • Cell Proliferation
  • Development and progression
  • Down-Regulation - genetics
  • Gene Expression Regulation, Neoplastic
  • Gene Knockdown Techniques
  • general
  • Humans
  • Life Sciences
  • Matrix Metalloproteinase 2 - genetics
  • Matrix Metalloproteinase 2 - metabolism
  • Matrix Metalloproteinase 9 - genetics
  • Matrix Metalloproteinase 9 - metabolism
  • metastasis
  • metastasis,bladder cancer, c-Met, microRNA-409-3p
  • MicroRNA
  • microRNA-409-3p
  • MicroRNAs - genetics
  • MicroRNAs - metabolism
  • MIGRACION
  • MIGRATION
  • Molecular Sequence Data
  • Neoplasm Invasiveness
  • Protein Binding - genetics
  • Proteins
  • Proto-Oncogene Proteins c-met - metabolism
  • RNA, Messenger - genetics
  • RNA, Messenger - metabolism
  • RNA, Small Interfering - metabolism
  • Transfection
  • Urinary Bladder Neoplasms - genetics
  • Urinary Bladder Neoplasms - pathology
  • 생물학
ispartof: Molecules and Cells, 36(1), pp.62-68
description: There is increasing evidence suggesting that dysregulation of certain microRNAs (miRNAs) may contribute to tumor progression and metastasis. Previous studies have shown that miR-409-3p is dysregulated in some malignancies, but its role in bladder cancer is still unknown. Here, we find that miR-409-3p is down-regulated in human bladder cancer tissues and cell lines. Enforced expression of miR-409-3p in bladder cancer cells significantly reduced their migration and invasion without affecting cell viability. Bioinformatics analysis identified the pro-metastatic gene c-Met as a potential miR-409-3p target. Further studies indicated that miR-409-3p suppressed the expression of c- Met by binding to its 3′-untranslated region. Silencing of c- Met by small interfering RNAs phenocopied the effects of miR-409-3p overexpression, whereas restoration of c-Met in bladder cancer cells bladder cancer cells overexpressing miR-409-3p, partially reversed the suppressive effects of miR-409-3p. We further showed that MMP2 and MMP9 may be downstream effector proteins of miR-409-3p. These findings indicate that miR-409-3p could be a potential tumor suppressor in bladder cancer.
language: eng
source:
identifier: ISSN: 1016-8478
fulltext: no_fulltext
issn:
  • 1016-8478
  • 0219-1032
url: Link


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titleMicroRNA-409-3p inhibits migration and invasion of bladder cancer cells via targeting c-Met
creatorXu, X., Zhejiang University, Qingchun , China ; Chen, H., Zhejiang University, Qingchun , China ; Lin, Y.W., Zhejiang University, Qingchun , China ; Hu, Z.H., Zhejiang University, Qingchun , China ; Mao, Y.Q., Zhejiang University, Qingchun , China ; Wu, J., Zhejiang University, Qingchun , China ; Xu, X.L., Zhejiang University, Qingchun , China ; Zhu, Y., Zhejiang University, Qingchun , China ; Li, S., Zhejiang University, Qingchun , China ; Zheng, X.Y., Zhejiang University, Qingchun , China ; Xie, L.P., Zhejiang University, Qingchun , China
creatorcontribXu, X., Zhejiang University, Qingchun , China ; Chen, H., Zhejiang University, Qingchun , China ; Lin, Y.W., Zhejiang University, Qingchun , China ; Hu, Z.H., Zhejiang University, Qingchun , China ; Mao, Y.Q., Zhejiang University, Qingchun , China ; Wu, J., Zhejiang University, Qingchun , China ; Xu, X.L., Zhejiang University, Qingchun , China ; Zhu, Y., Zhejiang University, Qingchun , China ; Li, S., Zhejiang University, Qingchun , China ; Zheng, X.Y., Zhejiang University, Qingchun , China ; Xie, L.P., Zhejiang University, Qingchun , China
descriptionThere is increasing evidence suggesting that dysregulation of certain microRNAs (miRNAs) may contribute to tumor progression and metastasis. Previous studies have shown that miR-409-3p is dysregulated in some malignancies, but its role in bladder cancer is still unknown. Here, we find that miR-409-3p is down-regulated in human bladder cancer tissues and cell lines. Enforced expression of miR-409-3p in bladder cancer cells significantly reduced their migration and invasion without affecting cell viability. Bioinformatics analysis identified the pro-metastatic gene c-Met as a potential miR-409-3p target. Further studies indicated that miR-409-3p suppressed the expression of c- Met by binding to its 3′-untranslated region. Silencing of c- Met by small interfering RNAs phenocopied the effects of miR-409-3p overexpression, whereas restoration of c-Met in bladder cancer cells bladder cancer cells overexpressing miR-409-3p, partially reversed the suppressive effects of miR-409-3p. We further showed that MMP2 and MMP9 may be downstream effector proteins of miR-409-3p. These findings indicate that miR-409-3p could be a potential tumor suppressor in bladder cancer.
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languageeng
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subject3' Untranslated Regions - genetics ; Analysis ; Article ; Articles ; Base Sequence ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Biotechnology ; Bladder cancer ; c-Met ; Cancer cells ; Cell Biology ; Cell Line, Tumor ; Cell Movement - genetics ; Cell Proliferation ; Development and progression ; Down-Regulation - genetics ; Gene Expression Regulation, Neoplastic ; Gene Knockdown Techniques ; general ; Humans ; Life Sciences ; Matrix Metalloproteinase 2 - genetics ; Matrix Metalloproteinase 2 - metabolism ; Matrix Metalloproteinase 9 - genetics ; Matrix Metalloproteinase 9 - metabolism ; metastasis ; metastasis,bladder cancer, c-Met, microRNA-409-3p ; MicroRNA ; microRNA-409-3p ; MicroRNAs - genetics ; MicroRNAs - metabolism ; MIGRACION ; MIGRATION ; Molecular Sequence Data ; Neoplasm Invasiveness ; Protein Binding - genetics ; Proteins ; Proto-Oncogene Proteins c-met - metabolism ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; RNA, Small Interfering - metabolism ; Transfection ; Urinary Bladder Neoplasms - genetics ; Urinary Bladder Neoplasms - pathology ; 생물학
ispartofMolecules and Cells, 36(1), pp.62-68
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0Xu, X., Zhejiang University, Qingchun , China
1Chen, H., Zhejiang University, Qingchun , China
2Lin, Y.W., Zhejiang University, Qingchun , China
3Hu, Z.H., Zhejiang University, Qingchun , China
4Mao, Y.Q., Zhejiang University, Qingchun , China
5Wu, J., Zhejiang University, Qingchun , China
6Xu, X.L., Zhejiang University, Qingchun , China
7Zhu, Y., Zhejiang University, Qingchun , China
8Li, S., Zhejiang University, Qingchun , China
9Zheng, X.Y., Zhejiang University, Qingchun , China
10Xie, L.P., Zhejiang University, Qingchun , China
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0MicroRNA-409-3p inhibits migration and invasion of bladder cancer cells via targeting c-Met
1Molecules and cells
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descriptionThere is increasing evidence suggesting that dysregulation of certain microRNAs (miRNAs) may contribute to tumor progression and metastasis. Previous studies have shown that miR-409-3p is dysregulated in some malignancies, but its role in bladder cancer is still unknown. Here, we find that miR-409-3p is down-regulated in human bladder cancer tissues and cell lines. Enforced expression of miR-409-3p in bladder cancer cells significantly reduced their migration and invasion without affecting cell viability. Bioinformatics analysis identified the pro-metastatic gene c-Met as a potential miR-409-3p target. Further studies indicated that miR-409-3p suppressed the expression of c- Met by binding to its 3′-untranslated region. Silencing of c- Met by small interfering RNAs phenocopied the effects of miR-409-3p overexpression, whereas restoration of c-Met in bladder cancer cells bladder cancer cells overexpressing miR-409-3p, partially reversed the suppressive effects of miR-409-3p. We further showed that MMP2 and MMP9 may be downstream effector proteins of miR-409-3p. These findings indicate that miR-409-3p could be a potential tumor suppressor in bladder cancer.
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03' Untranslated Regions - genetics
1Analysis
2Article
3Articles
4Base Sequence
5Biochemistry
6Biomedical and Life Sciences
7Biomedicine
8Biotechnology
9Bladder cancer
10c-Met
11Cancer cells
12Cell Biology
13Cell Line, Tumor
14Cell Movement - genetics
15Cell Proliferation
16Development and progression
17Down-Regulation - genetics
18Gene Expression Regulation, Neoplastic
19Gene Knockdown Techniques
20general
21Humans
22Life Sciences
23Matrix Metalloproteinase 2 - genetics
24Matrix Metalloproteinase 2 - metabolism
25Matrix Metalloproteinase 9 - genetics
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36Neoplasm Invasiveness
37Protein Binding - genetics
38Proteins
39Proto-Oncogene Proteins c-met - metabolism
40RNA, Messenger - genetics
41RNA, Messenger - metabolism
42RNA, Small Interfering - metabolism
43Transfection
44Urinary Bladder Neoplasms - genetics
45Urinary Bladder Neoplasms - pathology
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9Zheng, X.Y., Zhejiang University, Qingchun , China
10Xie, L.P., Zhejiang University, Qingchun , China
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titleMicroRNA-409-3p inhibits migration and invasion of bladder cancer cells via targeting c-Met
authorXu, X., Zhejiang University, Qingchun , China ; Chen, H., Zhejiang University, Qingchun , China ; Lin, Y.W., Zhejiang University, Qingchun , China ; Hu, Z.H., Zhejiang University, Qingchun , China ; Mao, Y.Q., Zhejiang University, Qingchun , China ; Wu, J., Zhejiang University, Qingchun , China ; Xu, X.L., Zhejiang University, Qingchun , China ; Zhu, Y., Zhejiang University, Qingchun , China ; Li, S., Zhejiang University, Qingchun , China ; Zheng, X.Y., Zhejiang University, Qingchun , China ; Xie, L.P., Zhejiang University, Qingchun , China
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03' Untranslated Regions - genetics
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7Biomedicine
8Biotechnology
9Bladder cancer
10c-Met
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19Gene Knockdown Techniques
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38Proteins
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40RNA, Messenger - genetics
41RNA, Messenger - metabolism
42RNA, Small Interfering - metabolism
43Transfection
44Urinary Bladder Neoplasms - genetics
45Urinary Bladder Neoplasms - pathology
46생물학
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0Xu, X., Zhejiang University, Qingchun , China
1Chen, H., Zhejiang University, Qingchun , China
2Lin, Y.W., Zhejiang University, Qingchun , China
3Hu, Z.H., Zhejiang University, Qingchun , China
4Mao, Y.Q., Zhejiang University, Qingchun , China
5Wu, J., Zhejiang University, Qingchun , China
6Xu, X.L., Zhejiang University, Qingchun , China
7Zhu, Y., Zhejiang University, Qingchun , China
8Li, S., Zhejiang University, Qingchun , China
9Zheng, X.Y., Zhejiang University, Qingchun , China
10Xie, L.P., Zhejiang University, Qingchun , China
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1Chen, H., Zhejiang University, Qingchun , China
2Lin, Y.W., Zhejiang University, Qingchun , China
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4Mao, Y.Q., Zhejiang University, Qingchun , China
5Wu, J., Zhejiang University, Qingchun , China
6Xu, X.L., Zhejiang University, Qingchun , China
7Zhu, Y., Zhejiang University, Qingchun , China
8Li, S., Zhejiang University, Qingchun , China
9Zheng, X.Y., Zhejiang University, Qingchun , China
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abstractThere is increasing evidence suggesting that dysregulation of certain microRNAs (miRNAs) may contribute to tumor progression and metastasis. Previous studies have shown that miR-409-3p is dysregulated in some malignancies, but its role in bladder cancer is still unknown. Here, we find that miR-409-3p is down-regulated in human bladder cancer tissues and cell lines. Enforced expression of miR-409-3p in bladder cancer cells significantly reduced their migration and invasion without affecting cell viability. Bioinformatics analysis identified the pro-metastatic gene c-Met as a potential miR-409-3p target. Further studies indicated that miR-409-3p suppressed the expression of c- Met by binding to its 3′-untranslated region. Silencing of c- Met by small interfering RNAs phenocopied the effects of miR-409-3p overexpression, whereas restoration of c-Met in bladder cancer cells bladder cancer cells overexpressing miR-409-3p, partially reversed the suppressive effects of miR-409-3p. We further showed that MMP2 and MMP9 may be downstream effector proteins of miR-409-3p. These findings indicate that miR-409-3p could be a potential tumor suppressor in bladder cancer.
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pubSpringer Netherlands
pmid23820886
doi10.1007/s10059-013-0044-7
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