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Mating compatibility and competitiveness of transgenic and wild type Aedes aegypti (L.) under contained semi-field conditions

We conducted the world’s first experiments under semi-field conditions (ACL-2 field house) to assess the mating competitiveness of genetically sterile RIDL male mosquitoes (513A strain). The field house is a state-of-the-art, fully-contained trial facility, simulating the living space for a househol... Full description

Journal Title: Transgenic research 2012-06-15, Vol.22 (1), p.47-57
Main Author: Lee, H. L
Other Authors: Vasan, Seshadri , Ahmad, Nazni Wasi , Idris, Iswarti , Hanum, Norhaida , Selvi, S , Alphey, Luke , Murad, Shahnaz
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: Dordrecht: Springer Netherlands
ID: ISSN: 0962-8819
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recordid: cdi_pascalfrancis_primary_27575036
title: Mating compatibility and competitiveness of transgenic and wild type Aedes aegypti (L.) under contained semi-field conditions
format: Article
creator:
  • Lee, H. L
  • Vasan, Seshadri
  • Ahmad, Nazni Wasi
  • Idris, Iswarti
  • Hanum, Norhaida
  • Selvi, S
  • Alphey, Luke
  • Murad, Shahnaz
subjects:
  • Aedes - genetics
  • Aedes - physiology
  • Animal Genetics and Genomics
  • Animals
  • Animals, Genetically Modified - genetics
  • Animals, Genetically Modified - physiology
  • Biological and medical sciences
  • Biomedical and Life Sciences
  • Biomedical Engineering/Biotechnology
  • Biomedicine
  • Biotechnology
  • Competitive Behavior
  • Female
  • Fundamental and applied biological sciences. Psychology
  • Genetic control
  • Genetic Engineering
  • Genetic technics
  • Human Genetics
  • Humans
  • Infertility, Male - genetics
  • Larva
  • Life Sciences
  • Malaria
  • Malaysia
  • Male
  • Mating competitiveness
  • Methods. Procedures. Technologies
  • Molecular Medicine
  • Mosquitoes
  • Original Paper
  • Plant Genetics & Genomics
  • Plant Genetics and Genomics
  • Plant Sciences
  • Reproduction - genetics
  • RIDL
  • Sexual Behavior, Animal - physiology
  • Transgenic Aedes aegypti
  • Transgenic animals and transgenic plants
  • Transgenics
ispartof: Transgenic research, 2012-06-15, Vol.22 (1), p.47-57
description: We conducted the world’s first experiments under semi-field conditions (ACL-2 field house) to assess the mating competitiveness of genetically sterile RIDL male mosquitoes (513A strain). The field house is a state-of-the-art, fully-contained trial facility, simulating the living space for a household of 2–4 people in Peninsular Malaysia. Ten genetically sterile RIDL male A. aegypti mosquitoes competed with ten wild type males inside this field house to mate with ten wild type females. Hatched larvae from mated females were screened under a fluorescent microscope for genetic markers to determine if they were fathered by RIDL male or wild type male, and all results were cross-checked by PCR. Two such experiments were conducted, each repeated sufficient number of times. All strains were on a Malaysian lab strain background for the first experiment, while the RIDL males alone were on a recently-colonised Mexican strain background for the second experiment. A total of 52 % of the matings were with RIDL males in the first experiment, while 45 % of the matings were with RIDL (Mexican) males in the second experiment. Statistically, this is not significantly different from 50 % of the matings expected to take place with RIDL males if the latter were as competitive as that of the wild type males. This shows that A. aegypti RIDL-513A has excellent mating competitiveness under semi-field conditions, verifying earlier trends obtained in small lab cages. We also observed high mating compatibility between recently-colonised Mexican RIDL males and lab-reared Malaysian wild type females.
language: eng
source:
identifier: ISSN: 0962-8819
fulltext: no_fulltext
issn:
  • 0962-8819
  • 1573-9368
url: Link


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titleMating compatibility and competitiveness of transgenic and wild type Aedes aegypti (L.) under contained semi-field conditions
creatorLee, H. L ; Vasan, Seshadri ; Ahmad, Nazni Wasi ; Idris, Iswarti ; Hanum, Norhaida ; Selvi, S ; Alphey, Luke ; Murad, Shahnaz
creatorcontribLee, H. L ; Vasan, Seshadri ; Ahmad, Nazni Wasi ; Idris, Iswarti ; Hanum, Norhaida ; Selvi, S ; Alphey, Luke ; Murad, Shahnaz
descriptionWe conducted the world’s first experiments under semi-field conditions (ACL-2 field house) to assess the mating competitiveness of genetically sterile RIDL male mosquitoes (513A strain). The field house is a state-of-the-art, fully-contained trial facility, simulating the living space for a household of 2–4 people in Peninsular Malaysia. Ten genetically sterile RIDL male A. aegypti mosquitoes competed with ten wild type males inside this field house to mate with ten wild type females. Hatched larvae from mated females were screened under a fluorescent microscope for genetic markers to determine if they were fathered by RIDL male or wild type male, and all results were cross-checked by PCR. Two such experiments were conducted, each repeated sufficient number of times. All strains were on a Malaysian lab strain background for the first experiment, while the RIDL males alone were on a recently-colonised Mexican strain background for the second experiment. A total of 52 % of the matings were with RIDL males in the first experiment, while 45 % of the matings were with RIDL (Mexican) males in the second experiment. Statistically, this is not significantly different from 50 % of the matings expected to take place with RIDL males if the latter were as competitive as that of the wild type males. This shows that A. aegypti RIDL-513A has excellent mating competitiveness under semi-field conditions, verifying earlier trends obtained in small lab cages. We also observed high mating compatibility between recently-colonised Mexican RIDL males and lab-reared Malaysian wild type females.
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subjectAedes - genetics ; Aedes - physiology ; Animal Genetics and Genomics ; Animals ; Animals, Genetically Modified - genetics ; Animals, Genetically Modified - physiology ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedical Engineering/Biotechnology ; Biomedicine ; Biotechnology ; Competitive Behavior ; Female ; Fundamental and applied biological sciences. Psychology ; Genetic control ; Genetic Engineering ; Genetic technics ; Human Genetics ; Humans ; Infertility, Male - genetics ; Larva ; Life Sciences ; Malaria ; Malaysia ; Male ; Mating competitiveness ; Methods. Procedures. Technologies ; Molecular Medicine ; Mosquitoes ; Original Paper ; Plant Genetics & Genomics ; Plant Genetics and Genomics ; Plant Sciences ; Reproduction - genetics ; RIDL ; Sexual Behavior, Animal - physiology ; Transgenic Aedes aegypti ; Transgenic animals and transgenic plants ; Transgenics
ispartofTransgenic research, 2012-06-15, Vol.22 (1), p.47-57
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1Vasan, Seshadri
2Ahmad, Nazni Wasi
3Idris, Iswarti
4Hanum, Norhaida
5Selvi, S
6Alphey, Luke
7Murad, Shahnaz
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descriptionWe conducted the world’s first experiments under semi-field conditions (ACL-2 field house) to assess the mating competitiveness of genetically sterile RIDL male mosquitoes (513A strain). The field house is a state-of-the-art, fully-contained trial facility, simulating the living space for a household of 2–4 people in Peninsular Malaysia. Ten genetically sterile RIDL male A. aegypti mosquitoes competed with ten wild type males inside this field house to mate with ten wild type females. Hatched larvae from mated females were screened under a fluorescent microscope for genetic markers to determine if they were fathered by RIDL male or wild type male, and all results were cross-checked by PCR. Two such experiments were conducted, each repeated sufficient number of times. All strains were on a Malaysian lab strain background for the first experiment, while the RIDL males alone were on a recently-colonised Mexican strain background for the second experiment. A total of 52 % of the matings were with RIDL males in the first experiment, while 45 % of the matings were with RIDL (Mexican) males in the second experiment. Statistically, this is not significantly different from 50 % of the matings expected to take place with RIDL males if the latter were as competitive as that of the wild type males. This shows that A. aegypti RIDL-513A has excellent mating competitiveness under semi-field conditions, verifying earlier trends obtained in small lab cages. We also observed high mating compatibility between recently-colonised Mexican RIDL males and lab-reared Malaysian wild type females.
subject
0Aedes - genetics
1Aedes - physiology
2Animal Genetics and Genomics
3Animals
4Animals, Genetically Modified - genetics
5Animals, Genetically Modified - physiology
6Biological and medical sciences
7Biomedical and Life Sciences
8Biomedical Engineering/Biotechnology
9Biomedicine
10Biotechnology
11Competitive Behavior
12Female
13Fundamental and applied biological sciences. Psychology
14Genetic control
15Genetic Engineering
16Genetic technics
17Human Genetics
18Humans
19Infertility, Male - genetics
20Larva
21Life Sciences
22Malaria
23Malaysia
24Male
25Mating competitiveness
26Methods. Procedures. Technologies
27Molecular Medicine
28Mosquitoes
29Original Paper
30Plant Genetics & Genomics
31Plant Genetics and Genomics
32Plant Sciences
33Reproduction - genetics
34RIDL
35Sexual Behavior, Animal - physiology
36Transgenic Aedes aegypti
37Transgenic animals and transgenic plants
38Transgenics
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titleMating compatibility and competitiveness of transgenic and wild type Aedes aegypti (L.) under contained semi-field conditions
authorLee, H. L ; Vasan, Seshadri ; Ahmad, Nazni Wasi ; Idris, Iswarti ; Hanum, Norhaida ; Selvi, S ; Alphey, Luke ; Murad, Shahnaz
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1Aedes - physiology
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7Biomedical and Life Sciences
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34RIDL
35Sexual Behavior, Animal - physiology
36Transgenic Aedes aegypti
37Transgenic animals and transgenic plants
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abstractWe conducted the world’s first experiments under semi-field conditions (ACL-2 field house) to assess the mating competitiveness of genetically sterile RIDL male mosquitoes (513A strain). The field house is a state-of-the-art, fully-contained trial facility, simulating the living space for a household of 2–4 people in Peninsular Malaysia. Ten genetically sterile RIDL male A. aegypti mosquitoes competed with ten wild type males inside this field house to mate with ten wild type females. Hatched larvae from mated females were screened under a fluorescent microscope for genetic markers to determine if they were fathered by RIDL male or wild type male, and all results were cross-checked by PCR. Two such experiments were conducted, each repeated sufficient number of times. All strains were on a Malaysian lab strain background for the first experiment, while the RIDL males alone were on a recently-colonised Mexican strain background for the second experiment. A total of 52 % of the matings were with RIDL males in the first experiment, while 45 % of the matings were with RIDL (Mexican) males in the second experiment. Statistically, this is not significantly different from 50 % of the matings expected to take place with RIDL males if the latter were as competitive as that of the wild type males. This shows that A. aegypti RIDL-513A has excellent mating competitiveness under semi-field conditions, verifying earlier trends obtained in small lab cages. We also observed high mating compatibility between recently-colonised Mexican RIDL males and lab-reared Malaysian wild type females.
copDordrecht
pubSpringer Netherlands
pmid22700207
doi10.1007/s11248-012-9625-z