schliessen

Filtern

 

Bibliotheken

The downregulation of miR-200c/141 promotes ZEB1/2 expression and gastric cancer progression

Gastric cancer is the fourth most common malignancy in the world. Although microRNA-200 (miR-200) family members are thought to play roles in tumorigenesis, their functions in carcinogenesis are tumor specific, and the underlying mechanism of action still remains elusive. Few studies to date have ad... Full description

Journal Title: Medical oncology (Northwood London, England), 2014, Vol.32 (1), p.428
Main Author: Zhou, Xinliang
Other Authors: Wang, Yudong , Shan, Baoen , Han, Jing , Zhu, Haifeng , Lv, Yalei , Fan, Xiaojie , Sang, Meixiang , Liu, Xian-De , Liu, Wei
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: Boston: Springer US
ID: ISSN: 1357-0560
Link: https://www.ncbi.nlm.nih.gov/pubmed/25502084
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: cdi_proquest_journals_1646568626
title: The downregulation of miR-200c/141 promotes ZEB1/2 expression and gastric cancer progression
format: Article
creator:
  • Zhou, Xinliang
  • Wang, Yudong
  • Shan, Baoen
  • Han, Jing
  • Zhu, Haifeng
  • Lv, Yalei
  • Fan, Xiaojie
  • Sang, Meixiang
  • Liu, Xian-De
  • Liu, Wei
subjects:
  • Aged
  • Blotting, Western
  • Bone morphogenetic proteins
  • Cancer
  • Cell Movement - genetics
  • Chemotherapy
  • CpG Islands - genetics
  • Development and progression
  • Disease Progression
  • Disease-Free Survival
  • DNA Methylation - genetics
  • Down-Regulation
  • Female
  • Gene Expression Regulation, Neoplastic - genetics
  • Hematology
  • Homeodomain Proteins - biosynthesis
  • Homeodomain Proteins - genetics
  • Humans
  • Internal Medicine
  • Male
  • Medicine
  • Medicine & Public Health
  • Methylation
  • MicroRNAs - biosynthesis
  • MicroRNAs - genetics
  • Middle Aged
  • Neoplasm Invasiveness - genetics
  • Oncology
  • Original Paper
  • Pathology
  • Real-Time Polymerase Chain Reaction
  • Repressor Proteins - biosynthesis
  • Repressor Proteins - genetics
  • Stomach cancer
  • Stomach Neoplasms - genetics
  • Stomach Neoplasms - mortality
  • Stomach Neoplasms - pathology
  • Transcription Factors - biosynthesis
  • Transcription Factors - genetics
  • Transforming growth factors
  • Zinc Finger E-box Binding Homeobox 2
  • Zinc Finger E-box-Binding Homeobox 1
ispartof: Medical oncology (Northwood, London, England), 2014, Vol.32 (1), p.428
description: Gastric cancer is the fourth most common malignancy in the world. Although microRNA-200 (miR-200) family members are thought to play roles in tumorigenesis, their functions in carcinogenesis are tumor specific, and the underlying mechanism of action still remains elusive. Few studies to date have addressed the dysregulation and function of miR-200 family members in gastric cancer progression. Here, we report that the miR-200 family members, miR-200c and miR-141, were significantly downregulated in gastric cancer specimens and gastric cancer cell lines. Importantly, on clinical samples, the expression of miR-200c and miR-141 was inversely correlated with TNM stage, tumor invasion depth (T), tumor embolus and disease-free survival. Wound-healing assay results showed that co-transfected miR-200c/141 could inhibit the migration and invasion capability of the gastric cell line SGC-7901. We also found that miR-200c and miR-141 directly targeted zinc finger E-box-binding homeobox 1/2 (ZEB1/2) and upregulated E-cadherin expression. In specimens from gastric cancer patients, reduced expression of miR-200c/141 was associated with increased expression of ZEB1 and/or ZEB2 . In addition, the downregulation of miR-200c and miR-141 was found to be due to a highly methylated CpG island located upstream of their genomic sequence and/or upregulated TGF-β signaling. Treatment with the chemotherapeutic agent decitabine, a known DNA methyltransferase inhibitor, increased miR-200c/141 expression and ameliorated decreased expression of miR-200c/141 induced by TGF-β in SGC-7901 cells. Our study revealed that miR-200c/141 was downregulated by CpG island methylation and TGF-β signaling, which decreased ZEB1/2 expression and increased E-cadherin expression to inhibit migration and invasion of gastric cancer cells and provides powerful evidence for the application of decitabine in gastric cancer treatment.
language: eng
source:
identifier: ISSN: 1357-0560
fulltext: no_fulltext
issn:
  • 1357-0560
  • 1559-131X
url: Link


@attributes
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
RANK2.5610068
LOCALfalse
PrimoNMBib
record
control
sourceidgale_proqu
recordidTN_cdi_proquest_journals_1646568626
sourceformatXML
sourcesystemPC
galeidA393212557
sourcerecordidA393212557
originalsourceidFETCH-LOGICAL-c491t-fc8046cd235ff51acca94beef09fefbc11be08177447b0d7b353c182946e47870
addsrcrecordideNp9kV1rFTEQhhdRbK3-AG9kwev0zORjPy5rqa1QEEoFESFks5M15ezmmOyh9t8323P8RCUXCZP3eWeYtyheIhwjQL1KyEFwBigZSN4w8ag4RKVahgI_Ps5voWoGqoKD4llKNwAcFW-fFgdcKeDQyMPi8_UXKvtwO0Uatmsz-zCVwZWjv2IcwK5QYrmJYQwzpfLT2Rtc8ZK-bSKltEjN1JeDSXP0trRmshQX9bD_fl48cWad6MX-Pio-vD27Pr1gl-_P352eXDIrW5yZsw3IyvZcKOcUGmtNKzsiB60j11nEjqDBupay7qCvO6GExYa3siJZNzUcFa93vrn31y2lWd-EbZxyS42VrFTVVLz6qRrMmrSfXJijsaNPVp-IVnDMW1m8jv-iyqen0dswkfO5_huAO8DGkFIkpzfRjybeaQS9xKR3Mekck15i0iIzr_YDb7uR-h_E91yyoP7D1Pr5IZ08jV__15rvyJRNp4HiL7v4J3QP_Furww
sourcetypeAggregation Database
isCDItrue
recordtypearticle
pqid1646568626
display
typearticle
titleThe downregulation of miR-200c/141 promotes ZEB1/2 expression and gastric cancer progression
creatorZhou, Xinliang ; Wang, Yudong ; Shan, Baoen ; Han, Jing ; Zhu, Haifeng ; Lv, Yalei ; Fan, Xiaojie ; Sang, Meixiang ; Liu, Xian-De ; Liu, Wei
creatorcontribZhou, Xinliang ; Wang, Yudong ; Shan, Baoen ; Han, Jing ; Zhu, Haifeng ; Lv, Yalei ; Fan, Xiaojie ; Sang, Meixiang ; Liu, Xian-De ; Liu, Wei
descriptionGastric cancer is the fourth most common malignancy in the world. Although microRNA-200 (miR-200) family members are thought to play roles in tumorigenesis, their functions in carcinogenesis are tumor specific, and the underlying mechanism of action still remains elusive. Few studies to date have addressed the dysregulation and function of miR-200 family members in gastric cancer progression. Here, we report that the miR-200 family members, miR-200c and miR-141, were significantly downregulated in gastric cancer specimens and gastric cancer cell lines. Importantly, on clinical samples, the expression of miR-200c and miR-141 was inversely correlated with TNM stage, tumor invasion depth (T), tumor embolus and disease-free survival. Wound-healing assay results showed that co-transfected miR-200c/141 could inhibit the migration and invasion capability of the gastric cell line SGC-7901. We also found that miR-200c and miR-141 directly targeted zinc finger E-box-binding homeobox 1/2 (ZEB1/2) and upregulated E-cadherin expression. In specimens from gastric cancer patients, reduced expression of miR-200c/141 was associated with increased expression of ZEB1 and/or ZEB2 . In addition, the downregulation of miR-200c and miR-141 was found to be due to a highly methylated CpG island located upstream of their genomic sequence and/or upregulated TGF-β signaling. Treatment with the chemotherapeutic agent decitabine, a known DNA methyltransferase inhibitor, increased miR-200c/141 expression and ameliorated decreased expression of miR-200c/141 induced by TGF-β in SGC-7901 cells. Our study revealed that miR-200c/141 was downregulated by CpG island methylation and TGF-β signaling, which decreased ZEB1/2 expression and increased E-cadherin expression to inhibit migration and invasion of gastric cancer cells and provides powerful evidence for the application of decitabine in gastric cancer treatment.
identifier
0ISSN: 1357-0560
1EISSN: 1559-131X
2DOI: 10.1007/s12032-014-0428-3
3PMID: 25502084
4CODEN: MONCEZ
languageeng
publisherBoston: Springer US
subjectAged ; Blotting, Western ; Bone morphogenetic proteins ; Cancer ; Cell Movement - genetics ; Chemotherapy ; CpG Islands - genetics ; Development and progression ; Disease Progression ; Disease-Free Survival ; DNA Methylation - genetics ; Down-Regulation ; Female ; Gene Expression Regulation, Neoplastic - genetics ; Hematology ; Homeodomain Proteins - biosynthesis ; Homeodomain Proteins - genetics ; Humans ; Internal Medicine ; Male ; Medicine ; Medicine & Public Health ; Methylation ; MicroRNAs - biosynthesis ; MicroRNAs - genetics ; Middle Aged ; Neoplasm Invasiveness - genetics ; Oncology ; Original Paper ; Pathology ; Real-Time Polymerase Chain Reaction ; Repressor Proteins - biosynthesis ; Repressor Proteins - genetics ; Stomach cancer ; Stomach Neoplasms - genetics ; Stomach Neoplasms - mortality ; Stomach Neoplasms - pathology ; Transcription Factors - biosynthesis ; Transcription Factors - genetics ; Transforming growth factors ; Zinc Finger E-box Binding Homeobox 2 ; Zinc Finger E-box-Binding Homeobox 1
ispartofMedical oncology (Northwood, London, England), 2014, Vol.32 (1), p.428
rights
0Springer Science+Business Media New York 2014
1COPYRIGHT 2015 Springer
2Springer Science+Business Media New York 2015
lds50peer_reviewed
citedbyFETCH-LOGICAL-c491t-fc8046cd235ff51acca94beef09fefbc11be08177447b0d7b353c182946e47870
citesFETCH-LOGICAL-c491t-fc8046cd235ff51acca94beef09fefbc11be08177447b0d7b353c182946e47870
links
openurl$$Topenurl_article
thumbnail$$Usyndetics_thumb_exl
backlink$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25502084$$D View this record in MEDLINE/PubMed
search
creatorcontrib
0Zhou, Xinliang
1Wang, Yudong
2Shan, Baoen
3Han, Jing
4Zhu, Haifeng
5Lv, Yalei
6Fan, Xiaojie
7Sang, Meixiang
8Liu, Xian-De
9Liu, Wei
title
0The downregulation of miR-200c/141 promotes ZEB1/2 expression and gastric cancer progression
1Medical oncology (Northwood, London, England)
addtitle
0Med Oncol
1Med Oncol
descriptionGastric cancer is the fourth most common malignancy in the world. Although microRNA-200 (miR-200) family members are thought to play roles in tumorigenesis, their functions in carcinogenesis are tumor specific, and the underlying mechanism of action still remains elusive. Few studies to date have addressed the dysregulation and function of miR-200 family members in gastric cancer progression. Here, we report that the miR-200 family members, miR-200c and miR-141, were significantly downregulated in gastric cancer specimens and gastric cancer cell lines. Importantly, on clinical samples, the expression of miR-200c and miR-141 was inversely correlated with TNM stage, tumor invasion depth (T), tumor embolus and disease-free survival. Wound-healing assay results showed that co-transfected miR-200c/141 could inhibit the migration and invasion capability of the gastric cell line SGC-7901. We also found that miR-200c and miR-141 directly targeted zinc finger E-box-binding homeobox 1/2 (ZEB1/2) and upregulated E-cadherin expression. In specimens from gastric cancer patients, reduced expression of miR-200c/141 was associated with increased expression of ZEB1 and/or ZEB2 . In addition, the downregulation of miR-200c and miR-141 was found to be due to a highly methylated CpG island located upstream of their genomic sequence and/or upregulated TGF-β signaling. Treatment with the chemotherapeutic agent decitabine, a known DNA methyltransferase inhibitor, increased miR-200c/141 expression and ameliorated decreased expression of miR-200c/141 induced by TGF-β in SGC-7901 cells. Our study revealed that miR-200c/141 was downregulated by CpG island methylation and TGF-β signaling, which decreased ZEB1/2 expression and increased E-cadherin expression to inhibit migration and invasion of gastric cancer cells and provides powerful evidence for the application of decitabine in gastric cancer treatment.
subject
0Aged
1Blotting, Western
2Bone morphogenetic proteins
3Cancer
4Cell Movement - genetics
5Chemotherapy
6CpG Islands - genetics
7Development and progression
8Disease Progression
9Disease-Free Survival
10DNA Methylation - genetics
11Down-Regulation
12Female
13Gene Expression Regulation, Neoplastic - genetics
14Hematology
15Homeodomain Proteins - biosynthesis
16Homeodomain Proteins - genetics
17Humans
18Internal Medicine
19Male
20Medicine
21Medicine & Public Health
22Methylation
23MicroRNAs - biosynthesis
24MicroRNAs - genetics
25Middle Aged
26Neoplasm Invasiveness - genetics
27Oncology
28Original Paper
29Pathology
30Real-Time Polymerase Chain Reaction
31Repressor Proteins - biosynthesis
32Repressor Proteins - genetics
33Stomach cancer
34Stomach Neoplasms - genetics
35Stomach Neoplasms - mortality
36Stomach Neoplasms - pathology
37Transcription Factors - biosynthesis
38Transcription Factors - genetics
39Transforming growth factors
40Zinc Finger E-box Binding Homeobox 2
41Zinc Finger E-box-Binding Homeobox 1
issn
01357-0560
11559-131X
fulltextfalse
rsrctypearticle
creationdate2014
recordtypearticle
recordideNp9kV1rFTEQhhdRbK3-AG9kwev0zORjPy5rqa1QEEoFESFks5M15ezmmOyh9t8323P8RCUXCZP3eWeYtyheIhwjQL1KyEFwBigZSN4w8ag4RKVahgI_Ps5voWoGqoKD4llKNwAcFW-fFgdcKeDQyMPi8_UXKvtwO0Uatmsz-zCVwZWjv2IcwK5QYrmJYQwzpfLT2Rtc8ZK-bSKltEjN1JeDSXP0trRmshQX9bD_fl48cWad6MX-Pio-vD27Pr1gl-_P352eXDIrW5yZsw3IyvZcKOcUGmtNKzsiB60j11nEjqDBupay7qCvO6GExYa3siJZNzUcFa93vrn31y2lWd-EbZxyS42VrFTVVLz6qRrMmrSfXJijsaNPVp-IVnDMW1m8jv-iyqen0dswkfO5_huAO8DGkFIkpzfRjybeaQS9xKR3Mekck15i0iIzr_YDb7uR-h_E91yyoP7D1Pr5IZ08jV__15rvyJRNp4HiL7v4J3QP_Furww
startdate20141212
enddate20141212
creator
0Zhou, Xinliang
1Wang, Yudong
2Shan, Baoen
3Han, Jing
4Zhu, Haifeng
5Lv, Yalei
6Fan, Xiaojie
7Sang, Meixiang
8Liu, Xian-De
9Liu, Wei
general
0Springer US
1Springer
2Springer Nature B.V
scope
0CGR
1CUY
2CVF
3ECM
4EIF
5NPM
6AAYXX
7CITATION
8BSHEE
93V.
107X7
117XB
1288E
138AO
148FI
158FJ
168FK
17ABUWG
18BENPR
19FYUFA
20GHDGH
21K9.
22M0S
23M1P
24PQEST
25PQQKQ
26PQUKI
27PRINS
sort
creationdate20141212
titleThe downregulation of miR-200c/141 promotes ZEB1/2 expression and gastric cancer progression
authorZhou, Xinliang ; Wang, Yudong ; Shan, Baoen ; Han, Jing ; Zhu, Haifeng ; Lv, Yalei ; Fan, Xiaojie ; Sang, Meixiang ; Liu, Xian-De ; Liu, Wei
facets
frbrtype5
frbrgroupidcdi_FETCH-LOGICAL-c491t-fc8046cd235ff51acca94beef09fefbc11be08177447b0d7b353c182946e47870
rsrctypearticles
prefilterarticles
languageeng
creationdate2014
topic
0Aged
1Blotting, Western
2Bone morphogenetic proteins
3Cancer
4Cell Movement - genetics
5Chemotherapy
6CpG Islands - genetics
7Development and progression
8Disease Progression
9Disease-Free Survival
10DNA Methylation - genetics
11Down-Regulation
12Female
13Gene Expression Regulation, Neoplastic - genetics
14Hematology
15Homeodomain Proteins - biosynthesis
16Homeodomain Proteins - genetics
17Humans
18Internal Medicine
19Male
20Medicine
21Medicine & Public Health
22Methylation
23MicroRNAs - biosynthesis
24MicroRNAs - genetics
25Middle Aged
26Neoplasm Invasiveness - genetics
27Oncology
28Original Paper
29Pathology
30Real-Time Polymerase Chain Reaction
31Repressor Proteins - biosynthesis
32Repressor Proteins - genetics
33Stomach cancer
34Stomach Neoplasms - genetics
35Stomach Neoplasms - mortality
36Stomach Neoplasms - pathology
37Transcription Factors - biosynthesis
38Transcription Factors - genetics
39Transforming growth factors
40Zinc Finger E-box Binding Homeobox 2
41Zinc Finger E-box-Binding Homeobox 1
toplevelpeer_reviewed
creatorcontrib
0Zhou, Xinliang
1Wang, Yudong
2Shan, Baoen
3Han, Jing
4Zhu, Haifeng
5Lv, Yalei
6Fan, Xiaojie
7Sang, Meixiang
8Liu, Xian-De
9Liu, Wei
collection
0Medline
1MEDLINE
2MEDLINE (Ovid)
3MEDLINE
4MEDLINE
5PubMed
6CrossRef
7Academic OneFile (A&I only)
8ProQuest Central (Corporate)
9Health & Medical Collection
10ProQuest Central (purchase pre-March 2016)
11Medical Database (Alumni Edition)
12ProQuest Pharma Collection
13Hospital Premium Collection
14Hospital Premium Collection (Alumni Edition)
15ProQuest Central (Alumni) (purchase pre-March 2016)
16ProQuest Central (Alumni Edition)
17ProQuest Central
18Health Research Premium Collection
19Health Research Premium Collection (Alumni)
20ProQuest Health & Medical Complete (Alumni)
21Health & Medical Collection (Alumni Edition)
22Medical Database
23ProQuest One Academic Eastern Edition
24ProQuest One Academic
25ProQuest One Academic UKI Edition
26ProQuest Central China
jtitleMedical oncology (Northwood, London, England)
delivery
delcategoryRemote Search Resource
fulltextno_fulltext
addata
au
0Zhou, Xinliang
1Wang, Yudong
2Shan, Baoen
3Han, Jing
4Zhu, Haifeng
5Lv, Yalei
6Fan, Xiaojie
7Sang, Meixiang
8Liu, Xian-De
9Liu, Wei
formatjournal
genrearticle
ristypeJOUR
atitleThe downregulation of miR-200c/141 promotes ZEB1/2 expression and gastric cancer progression
jtitleMedical oncology (Northwood, London, England)
stitleMed Oncol
addtitleMed Oncol
date2014-12-12
risdate2014
volume32
issue1
spage428
pages428-
issn1357-0560
eissn1559-131X
codenMONCEZ
abstractGastric cancer is the fourth most common malignancy in the world. Although microRNA-200 (miR-200) family members are thought to play roles in tumorigenesis, their functions in carcinogenesis are tumor specific, and the underlying mechanism of action still remains elusive. Few studies to date have addressed the dysregulation and function of miR-200 family members in gastric cancer progression. Here, we report that the miR-200 family members, miR-200c and miR-141, were significantly downregulated in gastric cancer specimens and gastric cancer cell lines. Importantly, on clinical samples, the expression of miR-200c and miR-141 was inversely correlated with TNM stage, tumor invasion depth (T), tumor embolus and disease-free survival. Wound-healing assay results showed that co-transfected miR-200c/141 could inhibit the migration and invasion capability of the gastric cell line SGC-7901. We also found that miR-200c and miR-141 directly targeted zinc finger E-box-binding homeobox 1/2 (ZEB1/2) and upregulated E-cadherin expression. In specimens from gastric cancer patients, reduced expression of miR-200c/141 was associated with increased expression of ZEB1 and/or ZEB2 . In addition, the downregulation of miR-200c and miR-141 was found to be due to a highly methylated CpG island located upstream of their genomic sequence and/or upregulated TGF-β signaling. Treatment with the chemotherapeutic agent decitabine, a known DNA methyltransferase inhibitor, increased miR-200c/141 expression and ameliorated decreased expression of miR-200c/141 induced by TGF-β in SGC-7901 cells. Our study revealed that miR-200c/141 was downregulated by CpG island methylation and TGF-β signaling, which decreased ZEB1/2 expression and increased E-cadherin expression to inhibit migration and invasion of gastric cancer cells and provides powerful evidence for the application of decitabine in gastric cancer treatment.
copBoston
pubSpringer US
pmid25502084
doi10.1007/s12032-014-0428-3