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Apple MdMYC2 reduces aluminum stress tolerance by directly regulating MdERF3 gene

Background and aims Jasmonate (JA) and ethylene are involved in the regulation of the aluminum (Al)-induced growth inhibition. Although it has been reported that JA enhances Al-induced root-growth inhibition, its role in the regulation of growth interplaying with ethylene is still not well understoo... Full description

Journal Title: Plant and soil 2017-05-29, Vol.418 (1-2), p.255-266
Main Author: An, Jian-Ping
Other Authors: Wang, Xiao-Na , Yao, Ji-Fang , Ren, Yi-Ran , You, Chun-Xiang , Wang, Xiao -Fei , Hao, Yu-Jin
Format: Electronic Article Electronic Article
Language: English
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Publisher: Cham: Springer International Publishing
ID: ISSN: 0032-079X
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recordid: cdi_proquest_journals_1940450540
title: Apple MdMYC2 reduces aluminum stress tolerance by directly regulating MdERF3 gene
format: Article
creator:
  • An, Jian-Ping
  • Wang, Xiao-Na
  • Yao, Ji-Fang
  • Ren, Yi-Ran
  • You, Chun-Xiang
  • Wang, Xiao -Fei
  • Hao, Yu-Jin
subjects:
  • Aluminum
  • Apple
  • Biomedical and Life Sciences
  • Biosynthesis
  • Crosstalk
  • Ecology
  • Ethylene
  • Functional analysis
  • Fusion protein
  • Gene expression
  • Genes
  • Genetic aspects
  • Health aspects
  • Inhibition
  • Jasmonic acid
  • Life Sciences
  • Plant Physiology
  • Plant Sciences
  • Polymerase chain reaction
  • Regular Article
  • Soil Science & Conservation
  • Stress
  • Stresses
  • Tobacco
ispartof: Plant and soil, 2017-05-29, Vol.418 (1-2), p.255-266
description: Background and aims Jasmonate (JA) and ethylene are involved in the regulation of the aluminum (Al)-induced growth inhibition. Although it has been reported that JA enhances Al-induced root-growth inhibition, its role in the regulation of growth interplaying with ethylene is still not well understood. In this study, we investigated the mechanism underlying the effect of apple MdMYC2 transcription factor on Al stress. Methods Overexpression lines were used for functional analysis. Real-time quantitative RT-PCR was used to examine the expression level of ethylene responsive genes. ChIP-PCR, EMSA, and Y1H assays were used to test whether MdMYC2-GST fusion protein could directly bind to MdERF3 promoter. Transient transactivation assays in tobacco leave were conducted to confirm whether MdMYC2 positively regulated the expression of MdERF3 . Results MdMYC2 negatively regulated Al tolerance with up-regulating the expression of ethylene responsive genes. Moreover, MdMYC2 was observed to bind to the promoter of MdERF3 , a positive regulator of ethylene biosynthesis, and directly activated its transcription. And applying the antagonist of ethylene biosynthesis, AVG, alleviated MdMYC2 -modulated growth inhibition in Al stress. Conclusions We consider that MdMYC2 protein directly interacts and promotes the transcript of MdERF3 to affect ethylene biosynthesis, thereby regulating the Al-mediated stress response. Our findings provide a deeper understanding of the crosstalk between JA and ethylene as well as JA-mediated growth inhibition in apple.
language: eng
source:
identifier: ISSN: 0032-079X
fulltext: no_fulltext
issn:
  • 0032-079X
  • 1573-5036
url: Link


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titleApple MdMYC2 reduces aluminum stress tolerance by directly regulating MdERF3 gene
creatorAn, Jian-Ping ; Wang, Xiao-Na ; Yao, Ji-Fang ; Ren, Yi-Ran ; You, Chun-Xiang ; Wang, Xiao -Fei ; Hao, Yu-Jin
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descriptionBackground and aims Jasmonate (JA) and ethylene are involved in the regulation of the aluminum (Al)-induced growth inhibition. Although it has been reported that JA enhances Al-induced root-growth inhibition, its role in the regulation of growth interplaying with ethylene is still not well understood. In this study, we investigated the mechanism underlying the effect of apple MdMYC2 transcription factor on Al stress. Methods Overexpression lines were used for functional analysis. Real-time quantitative RT-PCR was used to examine the expression level of ethylene responsive genes. ChIP-PCR, EMSA, and Y1H assays were used to test whether MdMYC2-GST fusion protein could directly bind to MdERF3 promoter. Transient transactivation assays in tobacco leave were conducted to confirm whether MdMYC2 positively regulated the expression of MdERF3 . Results MdMYC2 negatively regulated Al tolerance with up-regulating the expression of ethylene responsive genes. Moreover, MdMYC2 was observed to bind to the promoter of MdERF3 , a positive regulator of ethylene biosynthesis, and directly activated its transcription. And applying the antagonist of ethylene biosynthesis, AVG, alleviated MdMYC2 -modulated growth inhibition in Al stress. Conclusions We consider that MdMYC2 protein directly interacts and promotes the transcript of MdERF3 to affect ethylene biosynthesis, thereby regulating the Al-mediated stress response. Our findings provide a deeper understanding of the crosstalk between JA and ethylene as well as JA-mediated growth inhibition in apple.
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subjectAluminum ; Apple ; Biomedical and Life Sciences ; Biosynthesis ; Crosstalk ; Ecology ; Ethylene ; Functional analysis ; Fusion protein ; Gene expression ; Genes ; Genetic aspects ; Health aspects ; Inhibition ; Jasmonic acid ; Life Sciences ; Plant Physiology ; Plant Sciences ; Polymerase chain reaction ; Regular Article ; Soil Science & Conservation ; Stress ; Stresses ; Tobacco
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descriptionBackground and aims Jasmonate (JA) and ethylene are involved in the regulation of the aluminum (Al)-induced growth inhibition. Although it has been reported that JA enhances Al-induced root-growth inhibition, its role in the regulation of growth interplaying with ethylene is still not well understood. In this study, we investigated the mechanism underlying the effect of apple MdMYC2 transcription factor on Al stress. Methods Overexpression lines were used for functional analysis. Real-time quantitative RT-PCR was used to examine the expression level of ethylene responsive genes. ChIP-PCR, EMSA, and Y1H assays were used to test whether MdMYC2-GST fusion protein could directly bind to MdERF3 promoter. Transient transactivation assays in tobacco leave were conducted to confirm whether MdMYC2 positively regulated the expression of MdERF3 . Results MdMYC2 negatively regulated Al tolerance with up-regulating the expression of ethylene responsive genes. Moreover, MdMYC2 was observed to bind to the promoter of MdERF3 , a positive regulator of ethylene biosynthesis, and directly activated its transcription. And applying the antagonist of ethylene biosynthesis, AVG, alleviated MdMYC2 -modulated growth inhibition in Al stress. Conclusions We consider that MdMYC2 protein directly interacts and promotes the transcript of MdERF3 to affect ethylene biosynthesis, thereby regulating the Al-mediated stress response. Our findings provide a deeper understanding of the crosstalk between JA and ethylene as well as JA-mediated growth inhibition in apple.
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abstractBackground and aims Jasmonate (JA) and ethylene are involved in the regulation of the aluminum (Al)-induced growth inhibition. Although it has been reported that JA enhances Al-induced root-growth inhibition, its role in the regulation of growth interplaying with ethylene is still not well understood. In this study, we investigated the mechanism underlying the effect of apple MdMYC2 transcription factor on Al stress. Methods Overexpression lines were used for functional analysis. Real-time quantitative RT-PCR was used to examine the expression level of ethylene responsive genes. ChIP-PCR, EMSA, and Y1H assays were used to test whether MdMYC2-GST fusion protein could directly bind to MdERF3 promoter. Transient transactivation assays in tobacco leave were conducted to confirm whether MdMYC2 positively regulated the expression of MdERF3 . Results MdMYC2 negatively regulated Al tolerance with up-regulating the expression of ethylene responsive genes. Moreover, MdMYC2 was observed to bind to the promoter of MdERF3 , a positive regulator of ethylene biosynthesis, and directly activated its transcription. And applying the antagonist of ethylene biosynthesis, AVG, alleviated MdMYC2 -modulated growth inhibition in Al stress. Conclusions We consider that MdMYC2 protein directly interacts and promotes the transcript of MdERF3 to affect ethylene biosynthesis, thereby regulating the Al-mediated stress response. Our findings provide a deeper understanding of the crosstalk between JA and ethylene as well as JA-mediated growth inhibition in apple.
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pubSpringer International Publishing
doi10.1007/s11104-017-3297-7