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Knockdown of HBx by RNAi inhibits proliferation and enhances chemotherapy-induced apoptosis in hepatocellular carcinoma cells

The present study was designed to research on RNA interference hepatitis B virus x gene approach to hepatocellular carcinoma (HCC) therapy. Previously, we constructed and identified shRNA eukaryotic expression vectors (pshRNA-X220) specific to HBx gene, pshRNA-MOCK (control); and established HCC cel... Full description

Journal Title: Medical oncology (Northwood London, England), 2009-12-01, Vol.27 (4), p.1227-1233
Main Author: He, Yan
Other Authors: Sun, Hui-qing , He, Xing-e , Wang, Wen-long , Lei, Jian-hua
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: Boston: Springer US
ID: ISSN: 1357-0560
Link: https://www.ncbi.nlm.nih.gov/pubmed/19949899
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title: Knockdown of HBx by RNAi inhibits proliferation and enhances chemotherapy-induced apoptosis in hepatocellular carcinoma cells
format: Article
creator:
  • He, Yan
  • Sun, Hui-qing
  • He, Xing-e
  • Wang, Wen-long
  • Lei, Jian-hua
subjects:
  • 5-Fluorouracil
  • Analysis
  • Antimetabolites, Antineoplastic - therapeutic use
  • Antineoplastic Agents - therapeutic use
  • Apoptosis
  • Blotting, Western
  • Cancer
  • Carcinoma, Hepatocellular - metabolism
  • Carcinoma, Hepatocellular - pathology
  • Carcinoma, Hepatocellular - therapy
  • Cell Cycle
  • Cell Proliferation
  • Chemotherapy
  • Cholecystokinin
  • Cisplatin - therapeutic use
  • Female
  • Flow Cytometry
  • Fluorouracil
  • Fluorouracil - therapeutic use
  • Hematology
  • Hepatitis B virus
  • Hepatoma
  • Humans
  • Internal Medicine
  • Liver cancer
  • Liver Neoplasms - metabolism
  • Liver Neoplasms - pathology
  • Liver Neoplasms - therapy
  • Male
  • Medicine
  • Medicine & Public Health
  • Messenger RNA
  • Middle Aged
  • Oncology
  • Original Paper
  • Pathology
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger - genetics
  • RNA, Small Interfering - genetics
  • Trans-Activators - antagonists & inhibitors
  • Trans-Activators - genetics
  • Trans-Activators - metabolism
ispartof: Medical oncology (Northwood, London, England), 2009-12-01, Vol.27 (4), p.1227-1233
description: The present study was designed to research on RNA interference hepatitis B virus x gene approach to hepatocellular carcinoma (HCC) therapy. Previously, we constructed and identified shRNA eukaryotic expression vectors (pshRNA-X220) specific to HBx gene, pshRNA-MOCK (control); and established HCC cell lines with stable expression shRNA eukaryotic vector targeting HBx gene—21543 cell lines (MHCC97-H of expressing shRNA against HBx), HK3 cell lines (MHCC97-H by transfected with pshRNA-MOCK). We examined the expression of HBx gene after RNA interference by semi-quantitative RT-PCR and assessed the effect of HBx knocked down on cell growth by proliferation assay using kit-8 (CCK8). As well as, we analyzed cell cycle distribution by flowcytometry and examined cell apoptosis using TUNEL assay. The HBx mRNA expression level is reduced, and cells growth was significantly stopped in 21543 cell lines. Cells with HBx knockdown were more sensitive to 5-fluorouracil/cisplatin. RNA interfering HBx induced an obvious time and dose-dependent inhibitory in comparison with the control cells. Meanwhile, RNA interferenced targeting HBx, in combination with chemotherapy can effectively induce apoptosis in hepatocellular carcinoma cells and restricts cell proliferation.
language: eng
source:
identifier: ISSN: 1357-0560
fulltext: no_fulltext
issn:
  • 1357-0560
  • 1559-131X
url: Link


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titleKnockdown of HBx by RNAi inhibits proliferation and enhances chemotherapy-induced apoptosis in hepatocellular carcinoma cells
creatorHe, Yan ; Sun, Hui-qing ; He, Xing-e ; Wang, Wen-long ; Lei, Jian-hua
creatorcontribHe, Yan ; Sun, Hui-qing ; He, Xing-e ; Wang, Wen-long ; Lei, Jian-hua
descriptionThe present study was designed to research on RNA interference hepatitis B virus x gene approach to hepatocellular carcinoma (HCC) therapy. Previously, we constructed and identified shRNA eukaryotic expression vectors (pshRNA-X220) specific to HBx gene, pshRNA-MOCK (control); and established HCC cell lines with stable expression shRNA eukaryotic vector targeting HBx gene—21543 cell lines (MHCC97-H of expressing shRNA against HBx), HK3 cell lines (MHCC97-H by transfected with pshRNA-MOCK). We examined the expression of HBx gene after RNA interference by semi-quantitative RT-PCR and assessed the effect of HBx knocked down on cell growth by proliferation assay using kit-8 (CCK8). As well as, we analyzed cell cycle distribution by flowcytometry and examined cell apoptosis using TUNEL assay. The HBx mRNA expression level is reduced, and cells growth was significantly stopped in 21543 cell lines. Cells with HBx knockdown were more sensitive to 5-fluorouracil/cisplatin. RNA interfering HBx induced an obvious time and dose-dependent inhibitory in comparison with the control cells. Meanwhile, RNA interferenced targeting HBx, in combination with chemotherapy can effectively induce apoptosis in hepatocellular carcinoma cells and restricts cell proliferation.
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languageeng
publisherBoston: Springer US
subject5-Fluorouracil ; Analysis ; Antimetabolites, Antineoplastic - therapeutic use ; Antineoplastic Agents - therapeutic use ; Apoptosis ; Blotting, Western ; Cancer ; Carcinoma, Hepatocellular - metabolism ; Carcinoma, Hepatocellular - pathology ; Carcinoma, Hepatocellular - therapy ; Cell Cycle ; Cell Proliferation ; Chemotherapy ; Cholecystokinin ; Cisplatin - therapeutic use ; Female ; Flow Cytometry ; Fluorouracil ; Fluorouracil - therapeutic use ; Hematology ; Hepatitis B virus ; Hepatoma ; Humans ; Internal Medicine ; Liver cancer ; Liver Neoplasms - metabolism ; Liver Neoplasms - pathology ; Liver Neoplasms - therapy ; Male ; Medicine ; Medicine & Public Health ; Messenger RNA ; Middle Aged ; Oncology ; Original Paper ; Pathology ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; RNA, Small Interfering - genetics ; Trans-Activators - antagonists & inhibitors ; Trans-Activators - genetics ; Trans-Activators - metabolism
ispartofMedical oncology (Northwood, London, England), 2009-12-01, Vol.27 (4), p.1227-1233
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0Knockdown of HBx by RNAi inhibits proliferation and enhances chemotherapy-induced apoptosis in hepatocellular carcinoma cells
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descriptionThe present study was designed to research on RNA interference hepatitis B virus x gene approach to hepatocellular carcinoma (HCC) therapy. Previously, we constructed and identified shRNA eukaryotic expression vectors (pshRNA-X220) specific to HBx gene, pshRNA-MOCK (control); and established HCC cell lines with stable expression shRNA eukaryotic vector targeting HBx gene—21543 cell lines (MHCC97-H of expressing shRNA against HBx), HK3 cell lines (MHCC97-H by transfected with pshRNA-MOCK). We examined the expression of HBx gene after RNA interference by semi-quantitative RT-PCR and assessed the effect of HBx knocked down on cell growth by proliferation assay using kit-8 (CCK8). As well as, we analyzed cell cycle distribution by flowcytometry and examined cell apoptosis using TUNEL assay. The HBx mRNA expression level is reduced, and cells growth was significantly stopped in 21543 cell lines. Cells with HBx knockdown were more sensitive to 5-fluorouracil/cisplatin. RNA interfering HBx induced an obvious time and dose-dependent inhibitory in comparison with the control cells. Meanwhile, RNA interferenced targeting HBx, in combination with chemotherapy can effectively induce apoptosis in hepatocellular carcinoma cells and restricts cell proliferation.
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05-Fluorouracil
1Analysis
2Antimetabolites, Antineoplastic - therapeutic use
3Antineoplastic Agents - therapeutic use
4Apoptosis
5Blotting, Western
6Cancer
7Carcinoma, Hepatocellular - metabolism
8Carcinoma, Hepatocellular - pathology
9Carcinoma, Hepatocellular - therapy
10Cell Cycle
11Cell Proliferation
12Chemotherapy
13Cholecystokinin
14Cisplatin - therapeutic use
15Female
16Flow Cytometry
17Fluorouracil
18Fluorouracil - therapeutic use
19Hematology
20Hepatitis B virus
21Hepatoma
22Humans
23Internal Medicine
24Liver cancer
25Liver Neoplasms - metabolism
26Liver Neoplasms - pathology
27Liver Neoplasms - therapy
28Male
29Medicine
30Medicine & Public Health
31Messenger RNA
32Middle Aged
33Oncology
34Original Paper
35Pathology
36Reverse Transcriptase Polymerase Chain Reaction
37RNA, Messenger - genetics
38RNA, Small Interfering - genetics
39Trans-Activators - antagonists & inhibitors
40Trans-Activators - genetics
41Trans-Activators - metabolism
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authorHe, Yan ; Sun, Hui-qing ; He, Xing-e ; Wang, Wen-long ; Lei, Jian-hua
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05-Fluorouracil
1Analysis
2Antimetabolites, Antineoplastic - therapeutic use
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4Apoptosis
5Blotting, Western
6Cancer
7Carcinoma, Hepatocellular - metabolism
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39Trans-Activators - antagonists & inhibitors
40Trans-Activators - genetics
41Trans-Activators - metabolism
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atitleKnockdown of HBx by RNAi inhibits proliferation and enhances chemotherapy-induced apoptosis in hepatocellular carcinoma cells
jtitleMedical oncology (Northwood, London, England)
stitleMed Oncol
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date2009-12-01
risdate2009
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issue4
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pages1227-1233
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abstractThe present study was designed to research on RNA interference hepatitis B virus x gene approach to hepatocellular carcinoma (HCC) therapy. Previously, we constructed and identified shRNA eukaryotic expression vectors (pshRNA-X220) specific to HBx gene, pshRNA-MOCK (control); and established HCC cell lines with stable expression shRNA eukaryotic vector targeting HBx gene—21543 cell lines (MHCC97-H of expressing shRNA against HBx), HK3 cell lines (MHCC97-H by transfected with pshRNA-MOCK). We examined the expression of HBx gene after RNA interference by semi-quantitative RT-PCR and assessed the effect of HBx knocked down on cell growth by proliferation assay using kit-8 (CCK8). As well as, we analyzed cell cycle distribution by flowcytometry and examined cell apoptosis using TUNEL assay. The HBx mRNA expression level is reduced, and cells growth was significantly stopped in 21543 cell lines. Cells with HBx knockdown were more sensitive to 5-fluorouracil/cisplatin. RNA interfering HBx induced an obvious time and dose-dependent inhibitory in comparison with the control cells. Meanwhile, RNA interferenced targeting HBx, in combination with chemotherapy can effectively induce apoptosis in hepatocellular carcinoma cells and restricts cell proliferation.
copBoston
pubSpringer US
pmid19949899
doi10.1007/s12032-009-9363-0