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Molecular characterisation of the diazotrophic bacterial community in uninoculated and inoculated field-grown sugarcane (Saccharum sp.)

To identify active diazotrophs in sugarcane, 16S rRNA and nifH transcript analyses were applied. This should help to better understand the basis of the biological nitrogen fixation (BNF) activity of a high nitrogen fixing sugarcane variety. A field experiment using the sugarcane variety RB 867515 wa... Full description

Journal Title: Plant and soil 2012, Vol.356 (1-2), p.83-99
Main Author: Fischer, Doreen
Other Authors: Pfitzner, Barbara , Schmid, Michael , Simões-Araújo, Jean L , Reis, Veronica M , Pereira, William , Ormeño-Orrillo, Ernesto , Hai, Brigitte , Hofmann, Andreas , Schloter, Michael , Martinez-Romero, Esperanza , Baldani, Jose Ivo , Hartmann, Anton
Format: Electronic Article Electronic Article
Language: English
Subjects:
RNA
Publisher: Dordrecht: Springer
ID: ISSN: 0032-079X
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title: Molecular characterisation of the diazotrophic bacterial community in uninoculated and inoculated field-grown sugarcane (Saccharum sp.)
format: Article
creator:
  • Fischer, Doreen
  • Pfitzner, Barbara
  • Schmid, Michael
  • Simões-Araújo, Jean L
  • Reis, Veronica M
  • Pereira, William
  • Ormeño-Orrillo, Ernesto
  • Hai, Brigitte
  • Hofmann, Andreas
  • Schloter, Michael
  • Martinez-Romero, Esperanza
  • Baldani, Jose Ivo
  • Hartmann, Anton
subjects:
  • Bacteria
  • Biodiesel fuels
  • Biomedical and Life Sciences
  • Bradyrhizobium
  • Burkholderia
  • Complementary DNA
  • Ecology
  • Gels
  • Gluconacetobacter
  • Herbaspirillum
  • Life Sciences
  • Molecular structure
  • Nitrogen
  • Phylogenetics
  • Plant Physiology
  • Plant roots
  • Plant Sciences
  • Plants
  • Regular Article
  • Rhizobium
  • RNA
  • Saccharum
  • Soil Science & Conservation
  • Sugar cane
  • Sugarcane
ispartof: Plant and soil, 2012, Vol.356 (1-2), p.83-99
description: To identify active diazotrophs in sugarcane, 16S rRNA and nifH transcript analyses were applied. This should help to better understand the basis of the biological nitrogen fixation (BNF) activity of a high nitrogen fixing sugarcane variety. A field experiment using the sugarcane variety RB 867515 was conducted in Seropédica, RJ, Brazil, receiving the following treatments: unfertilised and fertilised controls without inoculation, unfertilised with inoculation. The fivestrain mixture developed by EMBRAPA-CNPAB was used as inoculum. Root and leaf sheath samples were harvested in the third year of cultivation to analyse the 16S rRNA and nifH transcript diversity. In addition to nifH expression from Gluconacetobacter spp. and Burkholderia spp., a wide diversity of nifH sequences from previously uncharacterised Ideonella/Herbaspirillum related phylotypes in sugarcane shoots as well as Bradyrhizobium sp. and Rhizobium sp. in roots was found. These results were confirmed using 16S cDNA analysis. From the inoculated bacteria, only nifH transcripts from G. diazotrophicus and B. tropica were detected in leaf sheaths and roots. Known as well as yet uncultivated diazotrophs were found active in sugarcane roots and stems using molecular analyses. Two strains of the inoculum mix were identified at the late summer harvest.
language: eng
source:
identifier: ISSN: 0032-079X
fulltext: no_fulltext
issn:
  • 0032-079X
  • 1573-5036
url: Link


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titleMolecular characterisation of the diazotrophic bacterial community in uninoculated and inoculated field-grown sugarcane (Saccharum sp.)
creatorFischer, Doreen ; Pfitzner, Barbara ; Schmid, Michael ; Simões-Araújo, Jean L ; Reis, Veronica M ; Pereira, William ; Ormeño-Orrillo, Ernesto ; Hai, Brigitte ; Hofmann, Andreas ; Schloter, Michael ; Martinez-Romero, Esperanza ; Baldani, Jose Ivo ; Hartmann, Anton
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descriptionTo identify active diazotrophs in sugarcane, 16S rRNA and nifH transcript analyses were applied. This should help to better understand the basis of the biological nitrogen fixation (BNF) activity of a high nitrogen fixing sugarcane variety. A field experiment using the sugarcane variety RB 867515 was conducted in Seropédica, RJ, Brazil, receiving the following treatments: unfertilised and fertilised controls without inoculation, unfertilised with inoculation. The fivestrain mixture developed by EMBRAPA-CNPAB was used as inoculum. Root and leaf sheath samples were harvested in the third year of cultivation to analyse the 16S rRNA and nifH transcript diversity. In addition to nifH expression from Gluconacetobacter spp. and Burkholderia spp., a wide diversity of nifH sequences from previously uncharacterised Ideonella/Herbaspirillum related phylotypes in sugarcane shoots as well as Bradyrhizobium sp. and Rhizobium sp. in roots was found. These results were confirmed using 16S cDNA analysis. From the inoculated bacteria, only nifH transcripts from G. diazotrophicus and B. tropica were detected in leaf sheaths and roots. Known as well as yet uncultivated diazotrophs were found active in sugarcane roots and stems using molecular analyses. Two strains of the inoculum mix were identified at the late summer harvest.
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subjectBacteria ; Biodiesel fuels ; Biomedical and Life Sciences ; Bradyrhizobium ; Burkholderia ; Complementary DNA ; Ecology ; Gels ; Gluconacetobacter ; Herbaspirillum ; Life Sciences ; Molecular structure ; Nitrogen ; Phylogenetics ; Plant Physiology ; Plant roots ; Plant Sciences ; Plants ; Regular Article ; Rhizobium ; RNA ; Saccharum ; Soil Science & Conservation ; Sugar cane ; Sugarcane
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descriptionTo identify active diazotrophs in sugarcane, 16S rRNA and nifH transcript analyses were applied. This should help to better understand the basis of the biological nitrogen fixation (BNF) activity of a high nitrogen fixing sugarcane variety. A field experiment using the sugarcane variety RB 867515 was conducted in Seropédica, RJ, Brazil, receiving the following treatments: unfertilised and fertilised controls without inoculation, unfertilised with inoculation. The fivestrain mixture developed by EMBRAPA-CNPAB was used as inoculum. Root and leaf sheath samples were harvested in the third year of cultivation to analyse the 16S rRNA and nifH transcript diversity. In addition to nifH expression from Gluconacetobacter spp. and Burkholderia spp., a wide diversity of nifH sequences from previously uncharacterised Ideonella/Herbaspirillum related phylotypes in sugarcane shoots as well as Bradyrhizobium sp. and Rhizobium sp. in roots was found. These results were confirmed using 16S cDNA analysis. From the inoculated bacteria, only nifH transcripts from G. diazotrophicus and B. tropica were detected in leaf sheaths and roots. Known as well as yet uncultivated diazotrophs were found active in sugarcane roots and stems using molecular analyses. Two strains of the inoculum mix were identified at the late summer harvest.
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titleMolecular characterisation of the diazotrophic bacterial community in uninoculated and inoculated field-grown sugarcane (Saccharum sp.)
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atitleMolecular characterisation of the diazotrophic bacterial community in uninoculated and inoculated field-grown sugarcane (Saccharum sp.)
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issn0032-079X
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abstractTo identify active diazotrophs in sugarcane, 16S rRNA and nifH transcript analyses were applied. This should help to better understand the basis of the biological nitrogen fixation (BNF) activity of a high nitrogen fixing sugarcane variety. A field experiment using the sugarcane variety RB 867515 was conducted in Seropédica, RJ, Brazil, receiving the following treatments: unfertilised and fertilised controls without inoculation, unfertilised with inoculation. The fivestrain mixture developed by EMBRAPA-CNPAB was used as inoculum. Root and leaf sheath samples were harvested in the third year of cultivation to analyse the 16S rRNA and nifH transcript diversity. In addition to nifH expression from Gluconacetobacter spp. and Burkholderia spp., a wide diversity of nifH sequences from previously uncharacterised Ideonella/Herbaspirillum related phylotypes in sugarcane shoots as well as Bradyrhizobium sp. and Rhizobium sp. in roots was found. These results were confirmed using 16S cDNA analysis. From the inoculated bacteria, only nifH transcripts from G. diazotrophicus and B. tropica were detected in leaf sheaths and roots. Known as well as yet uncultivated diazotrophs were found active in sugarcane roots and stems using molecular analyses. Two strains of the inoculum mix were identified at the late summer harvest.
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pubSpringer
doi10.1007/s11104-011-0812-0