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Homologous HAP5 subunit from Picea wilsonii improved tolerance to salt and decreased sensitivity to ABA in transformed Arabidopsis

HAP is a ubiquitous transcription factor family which consists of three distinct subunits, namely HAP2, HAP3, and HAP5. Among them, HAP2 and HAP3 subunits have been reported to be involved in plant response to abiotic stress. Here, a HAP5 subunit was identified from Picea wilsonii Mast, and transfor... Full description

Journal Title: Planta 2013-08-01, Vol.238 (2), p.345-356
Main Author: Li, Lingli
Other Authors: Yu, Yanli , Wei, Jing , Huang, Guixue , Zhang, Dun , Liu, Yong , Zhang, Lingyun
Format: Electronic Article Electronic Article
Language: English
Subjects:
RNA
Publisher: Berlin/Heidelberg: Springer-Verlag
ID: ISSN: 0032-0935
Link: https://www.ncbi.nlm.nih.gov/pubmed/23703145
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title: Homologous HAP5 subunit from Picea wilsonii improved tolerance to salt and decreased sensitivity to ABA in transformed Arabidopsis
format: Article
creator:
  • Li, Lingli
  • Yu, Yanli
  • Wei, Jing
  • Huang, Guixue
  • Zhang, Dun
  • Liu, Yong
  • Zhang, Lingyun
subjects:
  • Abscisic acid
  • Abscisic Acid - pharmacology
  • Agriculture
  • Amino Acid Sequence
  • Arabidopsis - drug effects
  • Arabidopsis - genetics
  • Arabidopsis - physiology
  • Arabidopsis thaliana
  • Base Sequence
  • Biomedical and Life Sciences
  • Drought
  • Ecology
  • Forest management
  • Forestry
  • Gene Expression Regulation, Plant
  • Genes
  • Genetic transcription
  • Germination
  • Life Sciences
  • Mannitol - pharmacology
  • Molecular Sequence Data
  • Mutation
  • Original Article
  • Osmotic Pressure
  • Phenotype
  • Phenotypes
  • Picea - genetics
  • Plant Growth Regulators - pharmacology
  • Plant Proteins - genetics
  • Plant Proteins - metabolism
  • Plant Sciences
  • Plants
  • Plants, Genetically Modified
  • RNA
  • Salinity
  • Seed germination
  • Seedlings
  • Seedlings - drug effects
  • Seedlings - genetics
  • Seedlings - physiology
  • Seeds - drug effects
  • Seeds - genetics
  • Seeds - physiology
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sodium Chloride - pharmacology
  • Stress, Physiological
  • Transcription factors
  • Transcription Factors - genetics
  • Transcription Factors - metabolism
  • Transgenic plants
ispartof: Planta, 2013-08-01, Vol.238 (2), p.345-356
description: HAP is a ubiquitous transcription factor family which consists of three distinct subunits, namely HAP2, HAP3, and HAP5. Among them, HAP2 and HAP3 subunits have been reported to be involved in plant response to abiotic stress. Here, a HAP5 subunit was identified from Picea wilsonii Mast, and transformed to Arabidopsis to investigate its functions in plant stress response. We found that transformed Arabidopsis with over-expressing PwHAP5 exhibited higher seed germination under salinity, osmotic and abscisic acid (ABA) stress treatment compared to Col-0 plants. The seedlings of transformed Arabidopsis also showed improved tolerance to salinity and decreased sensitivity to ABA treatment. Over-expression of PwHAP5 in Arabidopsis athap5 mutant rescued partly tolerance to NaCl, mannitol and ABA treatment. Furthermore, we examined transcription levels of several stressrelated genes in transformed seedlings. Among them, mRNA expression levels of COR15a, KIN1, DREB2A, and RD29A genes were substantially higher in transformed Arabidopsis than those in wild-type (Col-0) plants. Therefore, our data revealed that PwHAP5 plays positive roles in response to salinity, osmotic and ABA stress at different developmental stages in plants, respectively, via possibly regulating stress-related genes.
language: eng
source:
identifier: ISSN: 0032-0935
fulltext: no_fulltext
issn:
  • 0032-0935
  • 1432-2048
url: Link


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titleHomologous HAP5 subunit from Picea wilsonii improved tolerance to salt and decreased sensitivity to ABA in transformed Arabidopsis
creatorLi, Lingli ; Yu, Yanli ; Wei, Jing ; Huang, Guixue ; Zhang, Dun ; Liu, Yong ; Zhang, Lingyun
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descriptionHAP is a ubiquitous transcription factor family which consists of three distinct subunits, namely HAP2, HAP3, and HAP5. Among them, HAP2 and HAP3 subunits have been reported to be involved in plant response to abiotic stress. Here, a HAP5 subunit was identified from Picea wilsonii Mast, and transformed to Arabidopsis to investigate its functions in plant stress response. We found that transformed Arabidopsis with over-expressing PwHAP5 exhibited higher seed germination under salinity, osmotic and abscisic acid (ABA) stress treatment compared to Col-0 plants. The seedlings of transformed Arabidopsis also showed improved tolerance to salinity and decreased sensitivity to ABA treatment. Over-expression of PwHAP5 in Arabidopsis athap5 mutant rescued partly tolerance to NaCl, mannitol and ABA treatment. Furthermore, we examined transcription levels of several stressrelated genes in transformed seedlings. Among them, mRNA expression levels of COR15a, KIN1, DREB2A, and RD29A genes were substantially higher in transformed Arabidopsis than those in wild-type (Col-0) plants. Therefore, our data revealed that PwHAP5 plays positive roles in response to salinity, osmotic and ABA stress at different developmental stages in plants, respectively, via possibly regulating stress-related genes.
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subjectAbscisic acid ; Abscisic Acid - pharmacology ; Agriculture ; Amino Acid Sequence ; Arabidopsis - drug effects ; Arabidopsis - genetics ; Arabidopsis - physiology ; Arabidopsis thaliana ; Base Sequence ; Biomedical and Life Sciences ; Drought ; Ecology ; Forest management ; Forestry ; Gene Expression Regulation, Plant ; Genes ; Genetic transcription ; Germination ; Life Sciences ; Mannitol - pharmacology ; Molecular Sequence Data ; Mutation ; Original Article ; Osmotic Pressure ; Phenotype ; Phenotypes ; Picea - genetics ; Plant Growth Regulators - pharmacology ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Sciences ; Plants ; Plants, Genetically Modified ; RNA ; Salinity ; Seed germination ; Seedlings ; Seedlings - drug effects ; Seedlings - genetics ; Seedlings - physiology ; Seeds - drug effects ; Seeds - genetics ; Seeds - physiology ; Sequence Alignment ; Sequence Analysis, DNA ; Sodium Chloride - pharmacology ; Stress, Physiological ; Transcription factors ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transgenic plants
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descriptionHAP is a ubiquitous transcription factor family which consists of three distinct subunits, namely HAP2, HAP3, and HAP5. Among them, HAP2 and HAP3 subunits have been reported to be involved in plant response to abiotic stress. Here, a HAP5 subunit was identified from Picea wilsonii Mast, and transformed to Arabidopsis to investigate its functions in plant stress response. We found that transformed Arabidopsis with over-expressing PwHAP5 exhibited higher seed germination under salinity, osmotic and abscisic acid (ABA) stress treatment compared to Col-0 plants. The seedlings of transformed Arabidopsis also showed improved tolerance to salinity and decreased sensitivity to ABA treatment. Over-expression of PwHAP5 in Arabidopsis athap5 mutant rescued partly tolerance to NaCl, mannitol and ABA treatment. Furthermore, we examined transcription levels of several stressrelated genes in transformed seedlings. Among them, mRNA expression levels of COR15a, KIN1, DREB2A, and RD29A genes were substantially higher in transformed Arabidopsis than those in wild-type (Col-0) plants. Therefore, our data revealed that PwHAP5 plays positive roles in response to salinity, osmotic and ABA stress at different developmental stages in plants, respectively, via possibly regulating stress-related genes.
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3Amino Acid Sequence
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10Drought
11Ecology
12Forest management
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17Germination
18Life Sciences
19Mannitol - pharmacology
20Molecular Sequence Data
21Mutation
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23Osmotic Pressure
24Phenotype
25Phenotypes
26Picea - genetics
27Plant Growth Regulators - pharmacology
28Plant Proteins - genetics
29Plant Proteins - metabolism
30Plant Sciences
31Plants
32Plants, Genetically Modified
33RNA
34Salinity
35Seed germination
36Seedlings
37Seedlings - drug effects
38Seedlings - genetics
39Seedlings - physiology
40Seeds - drug effects
41Seeds - genetics
42Seeds - physiology
43Sequence Alignment
44Sequence Analysis, DNA
45Sodium Chloride - pharmacology
46Stress, Physiological
47Transcription factors
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49Transcription Factors - metabolism
50Transgenic plants
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titleHomologous HAP5 subunit from Picea wilsonii improved tolerance to salt and decreased sensitivity to ABA in transformed Arabidopsis
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1Abscisic Acid - pharmacology
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3Amino Acid Sequence
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5Arabidopsis - genetics
6Arabidopsis - physiology
7Arabidopsis thaliana
8Base Sequence
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10Drought
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13Forestry
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20Molecular Sequence Data
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43Sequence Alignment
44Sequence Analysis, DNA
45Sodium Chloride - pharmacology
46Stress, Physiological
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49Transcription Factors - metabolism
50Transgenic plants
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abstractHAP is a ubiquitous transcription factor family which consists of three distinct subunits, namely HAP2, HAP3, and HAP5. Among them, HAP2 and HAP3 subunits have been reported to be involved in plant response to abiotic stress. Here, a HAP5 subunit was identified from Picea wilsonii Mast, and transformed to Arabidopsis to investigate its functions in plant stress response. We found that transformed Arabidopsis with over-expressing PwHAP5 exhibited higher seed germination under salinity, osmotic and abscisic acid (ABA) stress treatment compared to Col-0 plants. The seedlings of transformed Arabidopsis also showed improved tolerance to salinity and decreased sensitivity to ABA treatment. Over-expression of PwHAP5 in Arabidopsis athap5 mutant rescued partly tolerance to NaCl, mannitol and ABA treatment. Furthermore, we examined transcription levels of several stressrelated genes in transformed seedlings. Among them, mRNA expression levels of COR15a, KIN1, DREB2A, and RD29A genes were substantially higher in transformed Arabidopsis than those in wild-type (Col-0) plants. Therefore, our data revealed that PwHAP5 plays positive roles in response to salinity, osmotic and ABA stress at different developmental stages in plants, respectively, via possibly regulating stress-related genes.
copBerlin/Heidelberg
pubSpringer-Verlag
pmid23703145
doi10.1007/s00425-013-1894-0