schliessen

Filtern

 

Bibliotheken

Salicylic acid alleviates copper toxicity in rice (Oryza sativa L.) seedlings by up-regulating antioxidative and glyoxalase systems

The present study investigated the effect of salicylic acid (SA) on toxic symptoms, lipid peroxidation, reactive oxygen species generation and responses of antioxidative and glyoxalase systems in rice seedlings grown hydroponically under copper (Cu) stress for 48 h. Exposures of 75 and 150 μM Cu 2+... Full description

Journal Title: Ecotoxicology (London) 2013-04-12, Vol.22 (6), p.959-973
Main Author: Mostofa, Mohammad Golam
Other Authors: Fujita, Masayuki
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: Boston: Springer US
ID: ISSN: 0963-9292
Link: https://www.ncbi.nlm.nih.gov/pubmed/23579392
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: cdi_proquest_miscellaneous_1419369611
title: Salicylic acid alleviates copper toxicity in rice (Oryza sativa L.) seedlings by up-regulating antioxidative and glyoxalase systems
format: Article
creator:
  • Mostofa, Mohammad Golam
  • Fujita, Masayuki
subjects:
  • Antioxidants
  • Antioxidants - pharmacology
  • Article
  • Ascorbate Peroxidases - metabolism
  • Ascorbic Acid - analysis
  • Catalase - metabolism
  • Chlorophyll
  • Chlorophyll - metabolism
  • Copper
  • Copper - toxicity
  • Earth and Environmental Science
  • Ecology
  • Ecotoxicology
  • Elevated
  • Environment
  • Environmental Management
  • general
  • Glutathione
  • Glutathione - analysis
  • Glutathione Peroxidase - metabolism
  • Glutathione Reductase - metabolism
  • Glutathione transferase
  • Glutathione Transferase - metabolism
  • Hydrogen peroxide
  • Hydrogen Peroxide - analysis
  • Lactoylglutathione Lyase - metabolism
  • Leaves
  • Lipoxygenases - metabolism
  • Malondialdehyde - analysis
  • Oryza - drug effects
  • Oryza - enzymology
  • Oxidative Stress - drug effects
  • Phenols
  • Plant Leaves - drug effects
  • Plant Leaves - enzymology
  • Plant Roots - drug effects
  • Plant Roots - metabolism
  • Proline
  • Proline - analysis
  • Reactive Oxygen Species - metabolism
  • Rice
  • Roots
  • Salicylic Acid - pharmacology
  • Seedlings - drug effects
  • Seedlings - enzymology
  • Stresses
  • Superoxide
  • Superoxide Dismutase - metabolism
  • Thiolester Hydrolases - metabolism
  • Thiols
  • Toxicity
  • Up-Regulation
ispartof: Ecotoxicology (London), 2013-04-12, Vol.22 (6), p.959-973
description: The present study investigated the effect of salicylic acid (SA) on toxic symptoms, lipid peroxidation, reactive oxygen species generation and responses of antioxidative and glyoxalase systems in rice seedlings grown hydroponically under copper (Cu) stress for 48 h. Exposures of 75 and 150 μM Cu 2+ caused toxicity symptoms (chlorosis, necrosis and rolling in leaves), sharp increases in malondialdehyde (MDA), hydrogen peroxide (H 2 O 2 ) contents and lipoxygenase (LOX) activity with concomitant reductions of chlorophyll (Chl) and relative water content (RWC). Both levels of Cu decreased ascorbic acid (AsA), glutathione (GSH), non-protein thiol (NPT) and proline contents in roots but rather increased in leaves except that AsA decreased in leaves too. These results together with overaccumulation of superoxide (O 2 •− ) and H 2 O 2 in leaves revealed that Cu exposures induced oxidative stress. Contrary, SA-pretreatment (100 μM for 24 h) reduced toxicity symptoms and diminished Cu-induced increases in LOX activity, H 2 O 2 , MDA and proline contents while the levels of RWC, Chl, AsA and redox ratios were elevated. Higher levels of GSH and NPT were also observed in roots of SA-pretreated Cu-exposed seedlings. SA-pretreatment also exerted its beneficial role by inhibiting the Cu upward process. Studies on antioxidant enzymes showed that SA further enhanced the activities of superoxide dismutase, ascorbate peroxidase, glutathione reductase and glutathione peroxidase, and also elevated the depressed activities of catalase, dehydroascorbate reductase and glutathione S -transferase particularly at 150 μM Cu 2+ stress. In addition, the activity of glyoxalase system (glyoxalase I and II) was further elevated by SA pretreatment in the Cu-exposed seedlings. These results concluded that SA-mediated retention of Cu in roots and enhanced capacity of both antioxidative and glyoxalase systems might be associated with the alleviation of Cu-toxicity in rice seedlings.
language: eng
source:
identifier: ISSN: 0963-9292
fulltext: no_fulltext
issn:
  • 0963-9292
  • 1573-3017
url: Link


@attributes
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
RANK2.6749148
LOCALfalse
PrimoNMBib
record
control
sourceidgale_proqu
recordidTN_cdi_proquest_miscellaneous_1419369611
sourceformatXML
sourcesystemPC
galeidA337341342
sourcerecordidA337341342
originalsourceidFETCH-LOGICAL-c434t-28135128168f575a7d0507e19c5e7d12a26c0e0fe4baed67139546e7219487730
addsrcrecordideNp9kc1u1DAUhSMEotPCA7BBXpZFBl_bicfLqgKKNFIXwNryODeRK8ce7KSasOXF8SiFBYvK8v_5jq7uqap3QLdAqfyYgbairSnwGqjk9elFtYGmHDgF-bLaUNXyWjHFLqrLnB8opUoK-rq6YLyRiiu2qX5_M97ZpUxirOuI8R4fnZkwExuPR0xkiidn3bQQF0hyFsn1fVp-GZLN5B4N2W8_kIzYeReGTA4LmY91wmH25TsMxITJFYPuLMZy68jgl3gy3mQkeckTjvlN9ao3PuPbp_2q-vH50_fbu3p__-Xr7c2-toKLqWY74A2Utd31jWyM7GhDJYKyDcoOmGGtpUh7FAeDXSuBq0a0KBkosZOS06vqevU9pvhzxjzp0WWL3puAcc4aBCjeqhagSLerdDAetQt9nJKxZXQ4OhsD9q6833AuuQAuWAFgBWyKOSfs9TG50aRFA9XnsPQali5h6XNY-lSY90_1zIcRu3_E33SKQP5nWoIonYyhVOP8s9ZsJXMxDQMm_RDnFEp3n4H-ANGcsl0
sourcetypeAggregation Database
isCDItrue
recordtypearticle
pqid1419369611
display
typearticle
titleSalicylic acid alleviates copper toxicity in rice (Oryza sativa L.) seedlings by up-regulating antioxidative and glyoxalase systems
creatorMostofa, Mohammad Golam ; Fujita, Masayuki
creatorcontribMostofa, Mohammad Golam ; Fujita, Masayuki
descriptionThe present study investigated the effect of salicylic acid (SA) on toxic symptoms, lipid peroxidation, reactive oxygen species generation and responses of antioxidative and glyoxalase systems in rice seedlings grown hydroponically under copper (Cu) stress for 48 h. Exposures of 75 and 150 μM Cu 2+ caused toxicity symptoms (chlorosis, necrosis and rolling in leaves), sharp increases in malondialdehyde (MDA), hydrogen peroxide (H 2 O 2 ) contents and lipoxygenase (LOX) activity with concomitant reductions of chlorophyll (Chl) and relative water content (RWC). Both levels of Cu decreased ascorbic acid (AsA), glutathione (GSH), non-protein thiol (NPT) and proline contents in roots but rather increased in leaves except that AsA decreased in leaves too. These results together with overaccumulation of superoxide (O 2 •− ) and H 2 O 2 in leaves revealed that Cu exposures induced oxidative stress. Contrary, SA-pretreatment (100 μM for 24 h) reduced toxicity symptoms and diminished Cu-induced increases in LOX activity, H 2 O 2 , MDA and proline contents while the levels of RWC, Chl, AsA and redox ratios were elevated. Higher levels of GSH and NPT were also observed in roots of SA-pretreated Cu-exposed seedlings. SA-pretreatment also exerted its beneficial role by inhibiting the Cu upward process. Studies on antioxidant enzymes showed that SA further enhanced the activities of superoxide dismutase, ascorbate peroxidase, glutathione reductase and glutathione peroxidase, and also elevated the depressed activities of catalase, dehydroascorbate reductase and glutathione S -transferase particularly at 150 μM Cu 2+ stress. In addition, the activity of glyoxalase system (glyoxalase I and II) was further elevated by SA pretreatment in the Cu-exposed seedlings. These results concluded that SA-mediated retention of Cu in roots and enhanced capacity of both antioxidative and glyoxalase systems might be associated with the alleviation of Cu-toxicity in rice seedlings.
identifier
0ISSN: 0963-9292
1EISSN: 1573-3017
2DOI: 10.1007/s10646-013-1073-x
3PMID: 23579392
languageeng
publisherBoston: Springer US
subjectAntioxidants ; Antioxidants - pharmacology ; Article ; Ascorbate Peroxidases - metabolism ; Ascorbic Acid - analysis ; Catalase - metabolism ; Chlorophyll ; Chlorophyll - metabolism ; Copper ; Copper - toxicity ; Earth and Environmental Science ; Ecology ; Ecotoxicology ; Elevated ; Environment ; Environmental Management ; general ; Glutathione ; Glutathione - analysis ; Glutathione Peroxidase - metabolism ; Glutathione Reductase - metabolism ; Glutathione transferase ; Glutathione Transferase - metabolism ; Hydrogen peroxide ; Hydrogen Peroxide - analysis ; Lactoylglutathione Lyase - metabolism ; Leaves ; Lipoxygenases - metabolism ; Malondialdehyde - analysis ; Oryza - drug effects ; Oryza - enzymology ; Oxidative Stress - drug effects ; Phenols ; Plant Leaves - drug effects ; Plant Leaves - enzymology ; Plant Roots - drug effects ; Plant Roots - metabolism ; Proline ; Proline - analysis ; Reactive Oxygen Species - metabolism ; Rice ; Roots ; Salicylic Acid - pharmacology ; Seedlings - drug effects ; Seedlings - enzymology ; Stresses ; Superoxide ; Superoxide Dismutase - metabolism ; Thiolester Hydrolases - metabolism ; Thiols ; Toxicity ; Up-Regulation
ispartofEcotoxicology (London), 2013-04-12, Vol.22 (6), p.959-973
rights
0Springer Science+Business Media New York 2013
1COPYRIGHT 2013 Springer
lds50peer_reviewed
citedbyFETCH-LOGICAL-c434t-28135128168f575a7d0507e19c5e7d12a26c0e0fe4baed67139546e7219487730
citesFETCH-LOGICAL-c434t-28135128168f575a7d0507e19c5e7d12a26c0e0fe4baed67139546e7219487730
links
openurl$$Topenurl_article
thumbnail$$Usyndetics_thumb_exl
backlink$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23579392$$D View this record in MEDLINE/PubMed
search
creatorcontrib
0Mostofa, Mohammad Golam
1Fujita, Masayuki
title
0Salicylic acid alleviates copper toxicity in rice (Oryza sativa L.) seedlings by up-regulating antioxidative and glyoxalase systems
1Ecotoxicology (London)
addtitle
0Ecotoxicology
1Ecotoxicology
descriptionThe present study investigated the effect of salicylic acid (SA) on toxic symptoms, lipid peroxidation, reactive oxygen species generation and responses of antioxidative and glyoxalase systems in rice seedlings grown hydroponically under copper (Cu) stress for 48 h. Exposures of 75 and 150 μM Cu 2+ caused toxicity symptoms (chlorosis, necrosis and rolling in leaves), sharp increases in malondialdehyde (MDA), hydrogen peroxide (H 2 O 2 ) contents and lipoxygenase (LOX) activity with concomitant reductions of chlorophyll (Chl) and relative water content (RWC). Both levels of Cu decreased ascorbic acid (AsA), glutathione (GSH), non-protein thiol (NPT) and proline contents in roots but rather increased in leaves except that AsA decreased in leaves too. These results together with overaccumulation of superoxide (O 2 •− ) and H 2 O 2 in leaves revealed that Cu exposures induced oxidative stress. Contrary, SA-pretreatment (100 μM for 24 h) reduced toxicity symptoms and diminished Cu-induced increases in LOX activity, H 2 O 2 , MDA and proline contents while the levels of RWC, Chl, AsA and redox ratios were elevated. Higher levels of GSH and NPT were also observed in roots of SA-pretreated Cu-exposed seedlings. SA-pretreatment also exerted its beneficial role by inhibiting the Cu upward process. Studies on antioxidant enzymes showed that SA further enhanced the activities of superoxide dismutase, ascorbate peroxidase, glutathione reductase and glutathione peroxidase, and also elevated the depressed activities of catalase, dehydroascorbate reductase and glutathione S -transferase particularly at 150 μM Cu 2+ stress. In addition, the activity of glyoxalase system (glyoxalase I and II) was further elevated by SA pretreatment in the Cu-exposed seedlings. These results concluded that SA-mediated retention of Cu in roots and enhanced capacity of both antioxidative and glyoxalase systems might be associated with the alleviation of Cu-toxicity in rice seedlings.
subject
0Antioxidants
1Antioxidants - pharmacology
2Article
3Ascorbate Peroxidases - metabolism
4Ascorbic Acid - analysis
5Catalase - metabolism
6Chlorophyll
7Chlorophyll - metabolism
8Copper
9Copper - toxicity
10Earth and Environmental Science
11Ecology
12Ecotoxicology
13Elevated
14Environment
15Environmental Management
16general
17Glutathione
18Glutathione - analysis
19Glutathione Peroxidase - metabolism
20Glutathione Reductase - metabolism
21Glutathione transferase
22Glutathione Transferase - metabolism
23Hydrogen peroxide
24Hydrogen Peroxide - analysis
25Lactoylglutathione Lyase - metabolism
26Leaves
27Lipoxygenases - metabolism
28Malondialdehyde - analysis
29Oryza - drug effects
30Oryza - enzymology
31Oxidative Stress - drug effects
32Phenols
33Plant Leaves - drug effects
34Plant Leaves - enzymology
35Plant Roots - drug effects
36Plant Roots - metabolism
37Proline
38Proline - analysis
39Reactive Oxygen Species - metabolism
40Rice
41Roots
42Salicylic Acid - pharmacology
43Seedlings - drug effects
44Seedlings - enzymology
45Stresses
46Superoxide
47Superoxide Dismutase - metabolism
48Thiolester Hydrolases - metabolism
49Thiols
50Toxicity
51Up-Regulation
issn
00963-9292
11573-3017
fulltextfalse
rsrctypearticle
creationdate2013
recordtypearticle
recordideNp9kc1u1DAUhSMEotPCA7BBXpZFBl_bicfLqgKKNFIXwNryODeRK8ce7KSasOXF8SiFBYvK8v_5jq7uqap3QLdAqfyYgbairSnwGqjk9elFtYGmHDgF-bLaUNXyWjHFLqrLnB8opUoK-rq6YLyRiiu2qX5_M97ZpUxirOuI8R4fnZkwExuPR0xkiidn3bQQF0hyFsn1fVp-GZLN5B4N2W8_kIzYeReGTA4LmY91wmH25TsMxITJFYPuLMZy68jgl3gy3mQkeckTjvlN9ao3PuPbp_2q-vH50_fbu3p__-Xr7c2-toKLqWY74A2Utd31jWyM7GhDJYKyDcoOmGGtpUh7FAeDXSuBq0a0KBkosZOS06vqevU9pvhzxjzp0WWL3puAcc4aBCjeqhagSLerdDAetQt9nJKxZXQ4OhsD9q6833AuuQAuWAFgBWyKOSfs9TG50aRFA9XnsPQali5h6XNY-lSY90_1zIcRu3_E33SKQP5nWoIonYyhVOP8s9ZsJXMxDQMm_RDnFEp3n4H-ANGcsl0
startdate20130412
enddate20130412
creator
0Mostofa, Mohammad Golam
1Fujita, Masayuki
general
0Springer US
1Springer
scope
0CGR
1CUY
2CVF
3ECM
4EIF
5NPM
6AAYXX
7CITATION
8BSHEE
98FD
10FR3
11KR7
sort
creationdate20130412
titleSalicylic acid alleviates copper toxicity in rice (Oryza sativa L.) seedlings by up-regulating antioxidative and glyoxalase systems
authorMostofa, Mohammad Golam ; Fujita, Masayuki
facets
frbrtype5
frbrgroupidcdi_FETCH-LOGICAL-c434t-28135128168f575a7d0507e19c5e7d12a26c0e0fe4baed67139546e7219487730
rsrctypearticles
prefilterarticles
languageeng
creationdate2013
topic
0Antioxidants
1Antioxidants - pharmacology
2Article
3Ascorbate Peroxidases - metabolism
4Ascorbic Acid - analysis
5Catalase - metabolism
6Chlorophyll
7Chlorophyll - metabolism
8Copper
9Copper - toxicity
10Earth and Environmental Science
11Ecology
12Ecotoxicology
13Elevated
14Environment
15Environmental Management
16general
17Glutathione
18Glutathione - analysis
19Glutathione Peroxidase - metabolism
20Glutathione Reductase - metabolism
21Glutathione transferase
22Glutathione Transferase - metabolism
23Hydrogen peroxide
24Hydrogen Peroxide - analysis
25Lactoylglutathione Lyase - metabolism
26Leaves
27Lipoxygenases - metabolism
28Malondialdehyde - analysis
29Oryza - drug effects
30Oryza - enzymology
31Oxidative Stress - drug effects
32Phenols
33Plant Leaves - drug effects
34Plant Leaves - enzymology
35Plant Roots - drug effects
36Plant Roots - metabolism
37Proline
38Proline - analysis
39Reactive Oxygen Species - metabolism
40Rice
41Roots
42Salicylic Acid - pharmacology
43Seedlings - drug effects
44Seedlings - enzymology
45Stresses
46Superoxide
47Superoxide Dismutase - metabolism
48Thiolester Hydrolases - metabolism
49Thiols
50Toxicity
51Up-Regulation
toplevelpeer_reviewed
creatorcontrib
0Mostofa, Mohammad Golam
1Fujita, Masayuki
collection
0Medline
1MEDLINE
2MEDLINE (Ovid)
3MEDLINE
4MEDLINE
5PubMed
6CrossRef
7Academic OneFile (A&I only)
8Technology Research Database
9Engineering Research Database
10Civil Engineering Abstracts
jtitleEcotoxicology (London)
delivery
delcategoryRemote Search Resource
fulltextno_fulltext
addata
au
0Mostofa, Mohammad Golam
1Fujita, Masayuki
formatjournal
genrearticle
ristypeJOUR
atitleSalicylic acid alleviates copper toxicity in rice (Oryza sativa L.) seedlings by up-regulating antioxidative and glyoxalase systems
jtitleEcotoxicology (London)
stitleEcotoxicology
addtitleEcotoxicology
date2013-04-12
risdate2013
volume22
issue6
spage959
epage973
pages959-973
issn0963-9292
eissn1573-3017
abstractThe present study investigated the effect of salicylic acid (SA) on toxic symptoms, lipid peroxidation, reactive oxygen species generation and responses of antioxidative and glyoxalase systems in rice seedlings grown hydroponically under copper (Cu) stress for 48 h. Exposures of 75 and 150 μM Cu 2+ caused toxicity symptoms (chlorosis, necrosis and rolling in leaves), sharp increases in malondialdehyde (MDA), hydrogen peroxide (H 2 O 2 ) contents and lipoxygenase (LOX) activity with concomitant reductions of chlorophyll (Chl) and relative water content (RWC). Both levels of Cu decreased ascorbic acid (AsA), glutathione (GSH), non-protein thiol (NPT) and proline contents in roots but rather increased in leaves except that AsA decreased in leaves too. These results together with overaccumulation of superoxide (O 2 •− ) and H 2 O 2 in leaves revealed that Cu exposures induced oxidative stress. Contrary, SA-pretreatment (100 μM for 24 h) reduced toxicity symptoms and diminished Cu-induced increases in LOX activity, H 2 O 2 , MDA and proline contents while the levels of RWC, Chl, AsA and redox ratios were elevated. Higher levels of GSH and NPT were also observed in roots of SA-pretreated Cu-exposed seedlings. SA-pretreatment also exerted its beneficial role by inhibiting the Cu upward process. Studies on antioxidant enzymes showed that SA further enhanced the activities of superoxide dismutase, ascorbate peroxidase, glutathione reductase and glutathione peroxidase, and also elevated the depressed activities of catalase, dehydroascorbate reductase and glutathione S -transferase particularly at 150 μM Cu 2+ stress. In addition, the activity of glyoxalase system (glyoxalase I and II) was further elevated by SA pretreatment in the Cu-exposed seedlings. These results concluded that SA-mediated retention of Cu in roots and enhanced capacity of both antioxidative and glyoxalase systems might be associated with the alleviation of Cu-toxicity in rice seedlings.
copBoston
pubSpringer US
pmid23579392
doi10.1007/s10646-013-1073-x