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Heterogeneous aerobic benzene-degrading communities in oxygen-depleted groundwaters

Abstract A sandstone aquifer beneath a petrochemicals plant (SIReN site, UK) is heterogeneously contaminated with benzene and oxygen-depleted. Despite low redox potentials in three of the most contaminated groundwaters (benzene concentrations from 17.8 to 294 mg L−1), we observed aerobic benzene deg... Full description

Journal Title: FEMS microbiology ecology 2006-11-01, Vol.58 (2), p.260-270
Main Author: Fahy, Anne
Other Authors: McGenity, Terry J , Timmis, Kenneth N , Ball, Andrew S
Format: Electronic Article Electronic Article
Language: English
Subjects:
DNA
Quelle: Alma/SFX Local Collection
Publisher: Oxford, UK: Blackwell Publishing Ltd
ID: ISSN: 0168-6496
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recordid: cdi_proquest_miscellaneous_14791625
title: Heterogeneous aerobic benzene-degrading communities in oxygen-depleted groundwaters
format: Article
creator:
  • Fahy, Anne
  • McGenity, Terry J
  • Timmis, Kenneth N
  • Ball, Andrew S
subjects:
  • Aerobes
  • Aerobic microorganisms
  • Aerobiosis
  • alkaline groundwater
  • Aquifers
  • Bacteria - classification
  • Bacteria - genetics
  • Bacteria - isolation & purification
  • Bacteria - metabolism
  • bacterial community composition
  • Bacteriology
  • Benzene
  • Benzene - metabolism
  • benzene contamination
  • Biodegradation
  • Biodegradation, Environmental
  • Biological and medical sciences
  • Cluster Analysis
  • Communities
  • Consortia
  • Degradation
  • Denitrification
  • Deoxyribonucleic acid
  • Depletion
  • DNA
  • DNA Fingerprinting
  • DNA, Bacterial - chemistry
  • DNA, Bacterial - genetics
  • DNA, Ribosomal - chemistry
  • DNA, Ribosomal - genetics
  • Ecosystem
  • Fundamental and applied biological sciences. Psychology
  • Genetic fingerprinting
  • Groundwater
  • Hydrocarbons
  • Hydrogen-Ion Concentration
  • Microbiology
  • Microcosms
  • Microorganisms
  • Miscellaneous
  • Molecular Sequence Data
  • Oxygen
  • Petrochemicals
  • Phylogeny
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Pseudomonas
  • Rhodococcus
  • RNA, Ribosomal, 16S - genetics
  • rRNA 16S
  • Sandstone
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid
  • Soil Microbiology
  • Strains (organisms)
  • terminal restriction fragment length polymorphism (T‐RFLP)
  • United Kingdom
  • Water Microbiology
  • Water, Underground
ispartof: FEMS microbiology ecology, 2006-11-01, Vol.58 (2), p.260-270
description: Abstract A sandstone aquifer beneath a petrochemicals plant (SIReN site, UK) is heterogeneously contaminated with benzene and oxygen-depleted. Despite low redox potentials in three of the most contaminated groundwaters (benzene concentrations from 17.8 to 294 mg L−1), we observed aerobic benzene degradation in microcosms, indicating the presence in situ of a latent community of obligate aerobic microorganisms or an active community of facultative aerobes responding rapidly to oxygen ingress. Moreover, benzene degradation occurred at the ambient pH of 8.9 and 9.4, considerably more alkaline conditions than previously reported. 16S rRNA analyses showed that the groundwater microcosm communities were distinct from each other, despite sharing the function of aerobic benzene degradation. From DNA fingerprinting, one consortium was dominated by Acidovorax spp., another by Pseudomonas spp.; these benzene-degrading consortia were similar to the in situ communities, perhaps indicating that these organisms are active in situ and degrading benzene microaerophilically or by denitrification. Conversely, in the third sample, benzene degradation occurred only after the community changed from a Rhodoferax-dominated community to a mix of Rhodococcus and Hydrogenophaga spp. Four of the main benzene-degrading strains were brought into culture: Hydrogenophaga and Pseudomonas spp., and two strains of Rhodococcus erythropolis, a ubiquitous and metabolically versatile organism.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0168-6496
fulltext: fulltext
issn:
  • 0168-6496
  • 1574-6941
url: Link


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titleHeterogeneous aerobic benzene-degrading communities in oxygen-depleted groundwaters
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descriptionAbstract A sandstone aquifer beneath a petrochemicals plant (SIReN site, UK) is heterogeneously contaminated with benzene and oxygen-depleted. Despite low redox potentials in three of the most contaminated groundwaters (benzene concentrations from 17.8 to 294 mg L−1), we observed aerobic benzene degradation in microcosms, indicating the presence in situ of a latent community of obligate aerobic microorganisms or an active community of facultative aerobes responding rapidly to oxygen ingress. Moreover, benzene degradation occurred at the ambient pH of 8.9 and 9.4, considerably more alkaline conditions than previously reported. 16S rRNA analyses showed that the groundwater microcosm communities were distinct from each other, despite sharing the function of aerobic benzene degradation. From DNA fingerprinting, one consortium was dominated by Acidovorax spp., another by Pseudomonas spp.; these benzene-degrading consortia were similar to the in situ communities, perhaps indicating that these organisms are active in situ and degrading benzene microaerophilically or by denitrification. Conversely, in the third sample, benzene degradation occurred only after the community changed from a Rhodoferax-dominated community to a mix of Rhodococcus and Hydrogenophaga spp. Four of the main benzene-degrading strains were brought into culture: Hydrogenophaga and Pseudomonas spp., and two strains of Rhodococcus erythropolis, a ubiquitous and metabolically versatile organism.
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languageeng
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subjectAerobes ; Aerobic microorganisms ; Aerobiosis ; alkaline groundwater ; Aquifers ; Bacteria - classification ; Bacteria - genetics ; Bacteria - isolation & purification ; Bacteria - metabolism ; bacterial community composition ; Bacteriology ; Benzene ; Benzene - metabolism ; benzene contamination ; Biodegradation ; Biodegradation, Environmental ; Biological and medical sciences ; Cluster Analysis ; Communities ; Consortia ; Degradation ; Denitrification ; Deoxyribonucleic acid ; Depletion ; DNA ; DNA Fingerprinting ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - genetics ; Ecosystem ; Fundamental and applied biological sciences. Psychology ; Genetic fingerprinting ; Groundwater ; Hydrocarbons ; Hydrogen-Ion Concentration ; Microbiology ; Microcosms ; Microorganisms ; Miscellaneous ; Molecular Sequence Data ; Oxygen ; Petrochemicals ; Phylogeny ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Pseudomonas ; Rhodococcus ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; Sandstone ; Sequence Analysis, DNA ; Sequence Homology, Nucleic Acid ; Soil Microbiology ; Strains (organisms) ; terminal restriction fragment length polymorphism (T‐RFLP) ; United Kingdom ; Water Microbiology ; Water, Underground
ispartofFEMS microbiology ecology, 2006-11-01, Vol.58 (2), p.260-270
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02006 Federation of European Microbiological Societies 2006
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descriptionAbstract A sandstone aquifer beneath a petrochemicals plant (SIReN site, UK) is heterogeneously contaminated with benzene and oxygen-depleted. Despite low redox potentials in three of the most contaminated groundwaters (benzene concentrations from 17.8 to 294 mg L−1), we observed aerobic benzene degradation in microcosms, indicating the presence in situ of a latent community of obligate aerobic microorganisms or an active community of facultative aerobes responding rapidly to oxygen ingress. Moreover, benzene degradation occurred at the ambient pH of 8.9 and 9.4, considerably more alkaline conditions than previously reported. 16S rRNA analyses showed that the groundwater microcosm communities were distinct from each other, despite sharing the function of aerobic benzene degradation. From DNA fingerprinting, one consortium was dominated by Acidovorax spp., another by Pseudomonas spp.; these benzene-degrading consortia were similar to the in situ communities, perhaps indicating that these organisms are active in situ and degrading benzene microaerophilically or by denitrification. Conversely, in the third sample, benzene degradation occurred only after the community changed from a Rhodoferax-dominated community to a mix of Rhodococcus and Hydrogenophaga spp. Four of the main benzene-degrading strains were brought into culture: Hydrogenophaga and Pseudomonas spp., and two strains of Rhodococcus erythropolis, a ubiquitous and metabolically versatile organism.
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0Aerobes
1Aerobic microorganisms
2Aerobiosis
3alkaline groundwater
4Aquifers
5Bacteria - classification
6Bacteria - genetics
7Bacteria - isolation & purification
8Bacteria - metabolism
9bacterial community composition
10Bacteriology
11Benzene
12Benzene - metabolism
13benzene contamination
14Biodegradation
15Biodegradation, Environmental
16Biological and medical sciences
17Cluster Analysis
18Communities
19Consortia
20Degradation
21Denitrification
22Deoxyribonucleic acid
23Depletion
24DNA
25DNA Fingerprinting
26DNA, Bacterial - chemistry
27DNA, Bacterial - genetics
28DNA, Ribosomal - chemistry
29DNA, Ribosomal - genetics
30Ecosystem
31Fundamental and applied biological sciences. Psychology
32Genetic fingerprinting
33Groundwater
34Hydrocarbons
35Hydrogen-Ion Concentration
36Microbiology
37Microcosms
38Microorganisms
39Miscellaneous
40Molecular Sequence Data
41Oxygen
42Petrochemicals
43Phylogeny
44Polymerase Chain Reaction
45Polymorphism, Restriction Fragment Length
46Pseudomonas
47Rhodococcus
48RNA, Ribosomal, 16S - genetics
49rRNA 16S
50Sandstone
51Sequence Analysis, DNA
52Sequence Homology, Nucleic Acid
53Soil Microbiology
54Strains (organisms)
55terminal restriction fragment length polymorphism (T‐RFLP)
56United Kingdom
57Water Microbiology
58Water, Underground
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8Bacteria - metabolism
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26DNA, Bacterial - chemistry
27DNA, Bacterial - genetics
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43Phylogeny
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46Pseudomonas
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48RNA, Ribosomal, 16S - genetics
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50Sandstone
51Sequence Analysis, DNA
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53Soil Microbiology
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atitleHeterogeneous aerobic benzene-degrading communities in oxygen-depleted groundwaters
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volume58
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0Andrew S. Ball, School of Biological Sciences, Flinders University of South Australia, GPO Box 2100, Adelaide, South Australia 5001, Australia.
1Editor: Max Häggblom
2Present address
abstractAbstract A sandstone aquifer beneath a petrochemicals plant (SIReN site, UK) is heterogeneously contaminated with benzene and oxygen-depleted. Despite low redox potentials in three of the most contaminated groundwaters (benzene concentrations from 17.8 to 294 mg L−1), we observed aerobic benzene degradation in microcosms, indicating the presence in situ of a latent community of obligate aerobic microorganisms or an active community of facultative aerobes responding rapidly to oxygen ingress. Moreover, benzene degradation occurred at the ambient pH of 8.9 and 9.4, considerably more alkaline conditions than previously reported. 16S rRNA analyses showed that the groundwater microcosm communities were distinct from each other, despite sharing the function of aerobic benzene degradation. From DNA fingerprinting, one consortium was dominated by Acidovorax spp., another by Pseudomonas spp.; these benzene-degrading consortia were similar to the in situ communities, perhaps indicating that these organisms are active in situ and degrading benzene microaerophilically or by denitrification. Conversely, in the third sample, benzene degradation occurred only after the community changed from a Rhodoferax-dominated community to a mix of Rhodococcus and Hydrogenophaga spp. Four of the main benzene-degrading strains were brought into culture: Hydrogenophaga and Pseudomonas spp., and two strains of Rhodococcus erythropolis, a ubiquitous and metabolically versatile organism.
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