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Restoration of stressor-induced calcium dysregulation and autophagy inhibition by polyphenol-rich açaí ( Euterpe spp. ) fruit pulp extracts in rodent brain cells in vitro

Abstract Objectives Oxidative damage to lipids, proteins, and nucleic acids in the brain often causes progressive neuronal degeneration and death that are the focal traits of chronic and acute pathologies, including those involving cognitive decline. The aim of this study was to investigate the spec... Full description

Journal Title: Nutrition (Burbank Los Angeles County, Calif.), 2014, Vol.30 (7), p.853-862
Main Author: Poulose, Shibu M., Ph.D
Other Authors: Fisher, Derek R., B.S , Bielinski, Donna F., Ph.D , Gomes, Stacey M., B.S , Rimando, Agnes M., Ph.D , Schauss, Alexander G., Ph.D , Shukitt-Hale, Barbara, Ph.D
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Alma/SFX Local Collection
Publisher: New York, NY: Elsevier Inc
ID: ISSN: 0899-9007
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title: Restoration of stressor-induced calcium dysregulation and autophagy inhibition by polyphenol-rich açaí ( Euterpe spp. ) fruit pulp extracts in rodent brain cells in vitro
format: Article
creator:
  • Poulose, Shibu M., Ph.D
  • Fisher, Derek R., B.S
  • Bielinski, Donna F., Ph.D
  • Gomes, Stacey M., B.S
  • Rimando, Agnes M., Ph.D
  • Schauss, Alexander G., Ph.D
  • Shukitt-Hale, Barbara, Ph.D
subjects:
  • Acids
  • Animals
  • Antioxidants
  • Antioxidants - pharmacology
  • Antioxidants - therapeutic use
  • Autophagy
  • Autophagy - drug effects
  • Açaí
  • Biological and medical sciences
  • Brain - cytology
  • Brain - drug effects
  • Brain - metabolism
  • Brain health
  • Brain research
  • Calcium
  • Calcium - metabolism
  • Cell Line
  • Cognition Disorders - drug therapy
  • Cognition Disorders - metabolism
  • Dendrites - drug effects
  • Diet
  • Dopamine
  • Euterpe - chemistry
  • Feeding. Feeding behavior
  • Flavonoids - analysis
  • Flavonoids - pharmacology
  • Flavonoids - therapeutic use
  • Fruit - chemistry
  • Fruits
  • Fundamental and applied biological sciences. Psychology
  • Gastroenterology and Hepatology
  • Homeostasis
  • Inflammation
  • Kinases
  • Laboratory animals
  • Mice
  • Neurons
  • Oxidative stress
  • Oxidative Stress - physiology
  • Phytochemicals
  • Phytochemistry
  • Phytotherapy
  • Plant Extracts - pharmacology
  • Plant Extracts - therapeutic use
  • Polyphenols
  • Polyphenols - pharmacology
  • Polyphenols - therapeutic use
  • Proteins
  • Proteins - metabolism
  • Rats, Sprague-Dawley
  • Signal Transduction
  • Species Specificity
  • Studies
  • Vertebrates: anatomy and physiology, studies on body, several organs or systems
ispartof: Nutrition (Burbank, Los Angeles County, Calif.), 2014, Vol.30 (7), p.853-862
description: Abstract Objectives Oxidative damage to lipids, proteins, and nucleic acids in the brain often causes progressive neuronal degeneration and death that are the focal traits of chronic and acute pathologies, including those involving cognitive decline. The aim of this study was to investigate the specific effects of both Euterpe oleracea and Euterpe precatoria açaí fruit pulp on restoring stressor-induced calcium dysregulation, stunted growth of basal dendrites, and autophagy inhibition using embryonic hippocampal and HT22 hippocampal neurons. Methods Water-soluble whole fruit pulp extracts from two açaí species were applied to rat primary neurons and HT22 hippocampal neurons with varied time and concentrations. Recovery of neurons from dopamine-induced Ca2+ dysregulation was measured by live cell imaging using fluorescent microscopy. The effect of açaí fruit pulp extracts on neurons following chemically-induced autophagy inhibition was measured using both immunofluorescence and immunohistochemical techniques. Results It has been postulated that at least part of the loss of cognitive function in aging may depend on a dysregulation in calcium ion (Ca2+ ) homeostasis and a loss of autophagy function in the brain, which affects numerous signaling pathways and alters protein homeostasis. In the present study, polyphenol-rich fruit pulp extracts from two species of açaí, Euterpe precatoria and Euterpe oleracea , when applied to rat hippocampal primary neuronal cells (E18), caused a significant ( P  
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0899-9007
fulltext: fulltext
issn:
  • 0899-9007
  • 1873-1244
url: Link


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titleRestoration of stressor-induced calcium dysregulation and autophagy inhibition by polyphenol-rich açaí ( Euterpe spp. ) fruit pulp extracts in rodent brain cells in vitro
sourceAlma/SFX Local Collection
creatorPoulose, Shibu M., Ph.D ; Fisher, Derek R., B.S ; Bielinski, Donna F., Ph.D ; Gomes, Stacey M., B.S ; Rimando, Agnes M., Ph.D ; Schauss, Alexander G., Ph.D ; Shukitt-Hale, Barbara, Ph.D
creatorcontribPoulose, Shibu M., Ph.D ; Fisher, Derek R., B.S ; Bielinski, Donna F., Ph.D ; Gomes, Stacey M., B.S ; Rimando, Agnes M., Ph.D ; Schauss, Alexander G., Ph.D ; Shukitt-Hale, Barbara, Ph.D
descriptionAbstract Objectives Oxidative damage to lipids, proteins, and nucleic acids in the brain often causes progressive neuronal degeneration and death that are the focal traits of chronic and acute pathologies, including those involving cognitive decline. The aim of this study was to investigate the specific effects of both Euterpe oleracea and Euterpe precatoria açaí fruit pulp on restoring stressor-induced calcium dysregulation, stunted growth of basal dendrites, and autophagy inhibition using embryonic hippocampal and HT22 hippocampal neurons. Methods Water-soluble whole fruit pulp extracts from two açaí species were applied to rat primary neurons and HT22 hippocampal neurons with varied time and concentrations. Recovery of neurons from dopamine-induced Ca2+ dysregulation was measured by live cell imaging using fluorescent microscopy. The effect of açaí fruit pulp extracts on neurons following chemically-induced autophagy inhibition was measured using both immunofluorescence and immunohistochemical techniques. Results It has been postulated that at least part of the loss of cognitive function in aging may depend on a dysregulation in calcium ion (Ca2+ ) homeostasis and a loss of autophagy function in the brain, which affects numerous signaling pathways and alters protein homeostasis. In the present study, polyphenol-rich fruit pulp extracts from two species of açaí, Euterpe precatoria and Euterpe oleracea , when applied to rat hippocampal primary neuronal cells (E18), caused a significant ( P  < 0.05) recovery of depolarized brain cells from dopamine-induced Ca2+ influx. Autophagy, a protein homeostasis mechanism in brain, when blocked by known inhibitors such as bafilomycin A1 or wortmannin, caused a significant reduction in the growth of primary basal dendrites in rodent primary hippocampal neurons and significant accumulation of polyubiquitinated proteins in mouse HT22 hippocampal neurons in culture. However, pretreatment with açaí extracts up to 1 mg/mL significantly increased the length of basal dendrites and attenuated the inhibitor-induced autophagy dysfunction. Açaí extracts activated the phosphorylation of mammalian target of rapamycin, increased the turnover of autophagosomes and MAP1 B LC3-II, and decreased accumulation of LC3-ubiquitin binding P62/SQSTM1. Conclusion Although the polyphenol profile of Euterpe precatoria showed substantially higher concentrations of major flavonoids han Euterpe oleracea , the relative effects were essentially similar for both species. The study adds to growing evidence that supports the putative health effects of açaí fruit species on brain cells.
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3PMID: 24985004
4CODEN: NUTRER
languageeng
publisherNew York, NY: Elsevier Inc
subjectAcids ; Animals ; Antioxidants ; Antioxidants - pharmacology ; Antioxidants - therapeutic use ; Autophagy ; Autophagy - drug effects ; Açaí ; Biological and medical sciences ; Brain - cytology ; Brain - drug effects ; Brain - metabolism ; Brain health ; Brain research ; Calcium ; Calcium - metabolism ; Cell Line ; Cognition Disorders - drug therapy ; Cognition Disorders - metabolism ; Dendrites - drug effects ; Diet ; Dopamine ; Euterpe - chemistry ; Feeding. Feeding behavior ; Flavonoids - analysis ; Flavonoids - pharmacology ; Flavonoids - therapeutic use ; Fruit - chemistry ; Fruits ; Fundamental and applied biological sciences. Psychology ; Gastroenterology and Hepatology ; Homeostasis ; Inflammation ; Kinases ; Laboratory animals ; Mice ; Neurons ; Oxidative stress ; Oxidative Stress - physiology ; Phytochemicals ; Phytochemistry ; Phytotherapy ; Plant Extracts - pharmacology ; Plant Extracts - therapeutic use ; Polyphenols ; Polyphenols - pharmacology ; Polyphenols - therapeutic use ; Proteins ; Proteins - metabolism ; Rats, Sprague-Dawley ; Signal Transduction ; Species Specificity ; Studies ; Vertebrates: anatomy and physiology, studies on body, several organs or systems
ispartofNutrition (Burbank, Los Angeles County, Calif.), 2014, Vol.30 (7), p.853-862
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1Fisher, Derek R., B.S
2Bielinski, Donna F., Ph.D
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5Schauss, Alexander G., Ph.D
6Shukitt-Hale, Barbara, Ph.D
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0Restoration of stressor-induced calcium dysregulation and autophagy inhibition by polyphenol-rich açaí ( Euterpe spp. ) fruit pulp extracts in rodent brain cells in vitro
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descriptionAbstract Objectives Oxidative damage to lipids, proteins, and nucleic acids in the brain often causes progressive neuronal degeneration and death that are the focal traits of chronic and acute pathologies, including those involving cognitive decline. The aim of this study was to investigate the specific effects of both Euterpe oleracea and Euterpe precatoria açaí fruit pulp on restoring stressor-induced calcium dysregulation, stunted growth of basal dendrites, and autophagy inhibition using embryonic hippocampal and HT22 hippocampal neurons. Methods Water-soluble whole fruit pulp extracts from two açaí species were applied to rat primary neurons and HT22 hippocampal neurons with varied time and concentrations. Recovery of neurons from dopamine-induced Ca2+ dysregulation was measured by live cell imaging using fluorescent microscopy. The effect of açaí fruit pulp extracts on neurons following chemically-induced autophagy inhibition was measured using both immunofluorescence and immunohistochemical techniques. Results It has been postulated that at least part of the loss of cognitive function in aging may depend on a dysregulation in calcium ion (Ca2+ ) homeostasis and a loss of autophagy function in the brain, which affects numerous signaling pathways and alters protein homeostasis. In the present study, polyphenol-rich fruit pulp extracts from two species of açaí, Euterpe precatoria and Euterpe oleracea , when applied to rat hippocampal primary neuronal cells (E18), caused a significant ( P  < 0.05) recovery of depolarized brain cells from dopamine-induced Ca2+ influx. Autophagy, a protein homeostasis mechanism in brain, when blocked by known inhibitors such as bafilomycin A1 or wortmannin, caused a significant reduction in the growth of primary basal dendrites in rodent primary hippocampal neurons and significant accumulation of polyubiquitinated proteins in mouse HT22 hippocampal neurons in culture. However, pretreatment with açaí extracts up to 1 mg/mL significantly increased the length of basal dendrites and attenuated the inhibitor-induced autophagy dysfunction. Açaí extracts activated the phosphorylation of mammalian target of rapamycin, increased the turnover of autophagosomes and MAP1 B LC3-II, and decreased accumulation of LC3-ubiquitin binding P62/SQSTM1. Conclusion Although the polyphenol profile of Euterpe precatoria showed substantially higher concentrations of major flavonoids han Euterpe oleracea , the relative effects were essentially similar for both species. The study adds to growing evidence that supports the putative health effects of açaí fruit species on brain cells.
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5Autophagy
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14Calcium
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16Cell Line
17Cognition Disorders - drug therapy
18Cognition Disorders - metabolism
19Dendrites - drug effects
20Diet
21Dopamine
22Euterpe - chemistry
23Feeding. Feeding behavior
24Flavonoids - analysis
25Flavonoids - pharmacology
26Flavonoids - therapeutic use
27Fruit - chemistry
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29Fundamental and applied biological sciences. Psychology
30Gastroenterology and Hepatology
31Homeostasis
32Inflammation
33Kinases
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37Oxidative stress
38Oxidative Stress - physiology
39Phytochemicals
40Phytochemistry
41Phytotherapy
42Plant Extracts - pharmacology
43Plant Extracts - therapeutic use
44Polyphenols
45Polyphenols - pharmacology
46Polyphenols - therapeutic use
47Proteins
48Proteins - metabolism
49Rats, Sprague-Dawley
50Signal Transduction
51Species Specificity
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53Vertebrates: anatomy and physiology, studies on body, several organs or systems
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titleRestoration of stressor-induced calcium dysregulation and autophagy inhibition by polyphenol-rich açaí ( Euterpe spp. ) fruit pulp extracts in rodent brain cells in vitro
authorPoulose, Shibu M., Ph.D ; Fisher, Derek R., B.S ; Bielinski, Donna F., Ph.D ; Gomes, Stacey M., B.S ; Rimando, Agnes M., Ph.D ; Schauss, Alexander G., Ph.D ; Shukitt-Hale, Barbara, Ph.D
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9Brain - cytology
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46Polyphenols - therapeutic use
47Proteins
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49Rats, Sprague-Dawley
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atitleRestoration of stressor-induced calcium dysregulation and autophagy inhibition by polyphenol-rich açaí ( Euterpe spp. ) fruit pulp extracts in rodent brain cells in vitro
jtitleNutrition (Burbank, Los Angeles County, Calif.)
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abstractAbstract Objectives Oxidative damage to lipids, proteins, and nucleic acids in the brain often causes progressive neuronal degeneration and death that are the focal traits of chronic and acute pathologies, including those involving cognitive decline. The aim of this study was to investigate the specific effects of both Euterpe oleracea and Euterpe precatoria açaí fruit pulp on restoring stressor-induced calcium dysregulation, stunted growth of basal dendrites, and autophagy inhibition using embryonic hippocampal and HT22 hippocampal neurons. Methods Water-soluble whole fruit pulp extracts from two açaí species were applied to rat primary neurons and HT22 hippocampal neurons with varied time and concentrations. Recovery of neurons from dopamine-induced Ca2+ dysregulation was measured by live cell imaging using fluorescent microscopy. The effect of açaí fruit pulp extracts on neurons following chemically-induced autophagy inhibition was measured using both immunofluorescence and immunohistochemical techniques. Results It has been postulated that at least part of the loss of cognitive function in aging may depend on a dysregulation in calcium ion (Ca2+ ) homeostasis and a loss of autophagy function in the brain, which affects numerous signaling pathways and alters protein homeostasis. In the present study, polyphenol-rich fruit pulp extracts from two species of açaí, Euterpe precatoria and Euterpe oleracea , when applied to rat hippocampal primary neuronal cells (E18), caused a significant ( P  < 0.05) recovery of depolarized brain cells from dopamine-induced Ca2+ influx. Autophagy, a protein homeostasis mechanism in brain, when blocked by known inhibitors such as bafilomycin A1 or wortmannin, caused a significant reduction in the growth of primary basal dendrites in rodent primary hippocampal neurons and significant accumulation of polyubiquitinated proteins in mouse HT22 hippocampal neurons in culture. However, pretreatment with açaí extracts up to 1 mg/mL significantly increased the length of basal dendrites and attenuated the inhibitor-induced autophagy dysfunction. Açaí extracts activated the phosphorylation of mammalian target of rapamycin, increased the turnover of autophagosomes and MAP1 B LC3-II, and decreased accumulation of LC3-ubiquitin binding P62/SQSTM1. Conclusion Although the polyphenol profile of Euterpe precatoria showed substantially higher concentrations of major flavonoids han Euterpe oleracea , the relative effects were essentially similar for both species. The study adds to growing evidence that supports the putative health effects of açaí fruit species on brain cells.
copNew York, NY
pubElsevier Inc
pmid24985004
doi10.1016/j.nut.2013.11.011