schliessen

Filtern

 

Bibliotheken

Plasma Exosome MicroRNA Profiling Unravels a New Potential Modulator of Adiponectin Pathway in Diabetes: Effect of Glycemic Control

Context: Type 2 diabetes is a chronic disease characterized by inadequate β-cell response to the progressive insulin resistance. MicroRNAs (miRNAs) are short, endogenous, noncoding RNAs representing a class of powerful gene expression modulators. Previous population studies observed a modulation of... Full description

Journal Title: The journal of clinical endocrinology and metabolism 2014, Vol.99 (9), p.E1681-E1685
Main Author: Santovito, Donato
Other Authors: De Nardis, Velia , Marcantonio, Pamela , Mandolini, Claudia , Paganelli, Camilla , Vitale, Elita , Buttitta, Fiamma , Bucci, Marco , Mezzetti, Andrea , Consoli, Agostino , Cipollone, Francesco
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: United States: Endocrine Society
ID: ISSN: 0021-972X
Link: https://www.ncbi.nlm.nih.gov/pubmed/24937531
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: cdi_proquest_miscellaneous_1560585612
title: Plasma Exosome MicroRNA Profiling Unravels a New Potential Modulator of Adiponectin Pathway in Diabetes: Effect of Glycemic Control
format: Article
creator:
  • Santovito, Donato
  • De Nardis, Velia
  • Marcantonio, Pamela
  • Mandolini, Claudia
  • Paganelli, Camilla
  • Vitale, Elita
  • Buttitta, Fiamma
  • Bucci, Marco
  • Mezzetti, Andrea
  • Consoli, Agostino
  • Cipollone, Francesco
subjects:
  • Abridged Index Medicus
  • Adiponectin - genetics
  • Adiponectin - metabolism
  • Aged
  • Case-Control Studies
  • Diabetes Mellitus, Type 2 - drug therapy
  • Diabetes Mellitus, Type 2 - genetics
  • Diabetes Mellitus, Type 2 - metabolism
  • Exosomes - genetics
  • Female
  • Humans
  • Hyperglycemia - drug therapy
  • Hyperglycemia - genetics
  • Hyperglycemia - metabolism
  • Hypoglycemic Agents - therapeutic use
  • Insulin Resistance - genetics
  • Male
  • MicroRNAs - blood
  • MicroRNAs - genetics
  • Middle Aged
  • Transcriptome
ispartof: The journal of clinical endocrinology and metabolism, 2014, Vol.99 (9), p.E1681-E1685
description: Context: Type 2 diabetes is a chronic disease characterized by inadequate β-cell response to the progressive insulin resistance. MicroRNAs (miRNAs) are short, endogenous, noncoding RNAs representing a class of powerful gene expression modulators. Previous population studies observed a modulation of circulating miRNAs in diabetic patients; however, few data are presently available on miRNA modulation in diabetic patients naïve to pharmacological treatment as well as the effect of glycemic control on this. Objective: We aimed at studying circulating miRNA expression in diabetic patients naïve to treatment and at investigating the influence on this of glycemic control. Design: This was a case-control study. Participants: Eighteen treatment-naïve diabetic patients with poor metabolic control and 12 control patients participated in the study. Main Outcome Measures: Wide miRNA expression profiling was performed, and the expression of miRNAs found to be dysregulated was then validated by quantitative RT-PCR. Finally, algorithm-identified putative miRNA targets were evaluated by quantitative RT-PCR and ELISA. Results: In diabetic patients, microarray analysis showed that four miRNAs are increased, whereas 21 miRNAs are decreased. Quantitative RT-PCR validation confirmed the significant up-regulation of miR-326 (P = .004) and down-regulation of let-7a (P < .001) and let-7f (P = .003). Notably, an inverse negative correlation was found between circulating miR-326 and its putative target adiponectin (p = −0.479, P = .009). After 12 months of antidiabetic treatment, quantitative RT-PCR data analysis showed that miR-326 levels were unaffected, whereas the levels of let-7a and let-7f were significantly increased. Conclusions: Treatment-naïve, poorly controlled diabetic patients show a significant dysregulation of miRNAs involved in the regulation of the adiponectin pathway, a phenomenon that may be reversed, at least in part, by improved glycemic control.
language: eng
source:
identifier: ISSN: 0021-972X
fulltext: no_fulltext
issn:
  • 0021-972X
  • 1945-7197
url: Link


@attributes
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
RANK2.7209532
LOCALfalse
PrimoNMBib
record
control
sourceidproquest_cross
recordidTN_cdi_proquest_miscellaneous_1560585612
sourceformatXML
sourcesystemPC
sourcerecordid1560585612
originalsourceidFETCH-LOGICAL-1383t-b708d2076c8ea454cf70e931e928644b325a5a4140ec8f6e6547c5e0d735b2f40
addsrcrecordideNp1kD1vFDEQQC1ERC6Bjhq5pGCDP_eD7nRcAlISTohIdJbXOws-ee2L7SVczR9nVxcooqSakebNKx5Cryk5o4yS91tzxgjlBa8Ff4YWtBGyqGhTPUcLQhgtmop9P0YnKW0JoUJI_gIdM9HwSnK6QH82TqdB4_XvkMIA-MqaGL5eL_Emht4663_gGx_1L3AJa3wNd3gTMvhstcNXoRudziHi0ONlZ3fBg8nW443OP-_0Hk_rR6tbyJA-4HXfT9cZvXB7A4M1eBV8jsG9REe9dgle3c9TdHO-_rb6VFx-ufi8Wl4WlNc8F21F6o6RqjQ1aCGF6SsCDafQsLoUouVMaqkFFQRM3ZdQSlEZCaSruGxZL8gpenvw7mK4HSFlNdhkwDntIYxJUVkSWcuSsgl9d0CnGilF6NUu2kHHvaJEzdnV1qg5u5qzT_ibe_PYDtD9h_91ngD2wGds1tnOAbR1T1n54Ql8F0y0HnYRUlLbMEY_hXr86y9Ko59B
sourcetypeAggregation Database
isCDItrue
recordtypearticle
pqid1560585612
display
typearticle
titlePlasma Exosome MicroRNA Profiling Unravels a New Potential Modulator of Adiponectin Pathway in Diabetes: Effect of Glycemic Control
creatorSantovito, Donato ; De Nardis, Velia ; Marcantonio, Pamela ; Mandolini, Claudia ; Paganelli, Camilla ; Vitale, Elita ; Buttitta, Fiamma ; Bucci, Marco ; Mezzetti, Andrea ; Consoli, Agostino ; Cipollone, Francesco
creatorcontribSantovito, Donato ; De Nardis, Velia ; Marcantonio, Pamela ; Mandolini, Claudia ; Paganelli, Camilla ; Vitale, Elita ; Buttitta, Fiamma ; Bucci, Marco ; Mezzetti, Andrea ; Consoli, Agostino ; Cipollone, Francesco
descriptionContext: Type 2 diabetes is a chronic disease characterized by inadequate β-cell response to the progressive insulin resistance. MicroRNAs (miRNAs) are short, endogenous, noncoding RNAs representing a class of powerful gene expression modulators. Previous population studies observed a modulation of circulating miRNAs in diabetic patients; however, few data are presently available on miRNA modulation in diabetic patients naïve to pharmacological treatment as well as the effect of glycemic control on this. Objective: We aimed at studying circulating miRNA expression in diabetic patients naïve to treatment and at investigating the influence on this of glycemic control. Design: This was a case-control study. Participants: Eighteen treatment-naïve diabetic patients with poor metabolic control and 12 control patients participated in the study. Main Outcome Measures: Wide miRNA expression profiling was performed, and the expression of miRNAs found to be dysregulated was then validated by quantitative RT-PCR. Finally, algorithm-identified putative miRNA targets were evaluated by quantitative RT-PCR and ELISA. Results: In diabetic patients, microarray analysis showed that four miRNAs are increased, whereas 21 miRNAs are decreased. Quantitative RT-PCR validation confirmed the significant up-regulation of miR-326 (P = .004) and down-regulation of let-7a (P < .001) and let-7f (P = .003). Notably, an inverse negative correlation was found between circulating miR-326 and its putative target adiponectin (p = −0.479, P = .009). After 12 months of antidiabetic treatment, quantitative RT-PCR data analysis showed that miR-326 levels were unaffected, whereas the levels of let-7a and let-7f were significantly increased. Conclusions: Treatment-naïve, poorly controlled diabetic patients show a significant dysregulation of miRNAs involved in the regulation of the adiponectin pathway, a phenomenon that may be reversed, at least in part, by improved glycemic control.
identifier
0ISSN: 0021-972X
1EISSN: 1945-7197
2DOI: 10.1210/jc.2013-3843
3PMID: 24937531
languageeng
publisherUnited States: Endocrine Society
subjectAbridged Index Medicus ; Adiponectin - genetics ; Adiponectin - metabolism ; Aged ; Case-Control Studies ; Diabetes Mellitus, Type 2 - drug therapy ; Diabetes Mellitus, Type 2 - genetics ; Diabetes Mellitus, Type 2 - metabolism ; Exosomes - genetics ; Female ; Humans ; Hyperglycemia - drug therapy ; Hyperglycemia - genetics ; Hyperglycemia - metabolism ; Hypoglycemic Agents - therapeutic use ; Insulin Resistance - genetics ; Male ; MicroRNAs - blood ; MicroRNAs - genetics ; Middle Aged ; Transcriptome
ispartofThe journal of clinical endocrinology and metabolism, 2014, Vol.99 (9), p.E1681-E1685
rightsCopyright © 2014 by the Endocrine Society
lds50peer_reviewed
oafree_for_read
citedbyFETCH-LOGICAL-1383t-b708d2076c8ea454cf70e931e928644b325a5a4140ec8f6e6547c5e0d735b2f40
citesFETCH-LOGICAL-1383t-b708d2076c8ea454cf70e931e928644b325a5a4140ec8f6e6547c5e0d735b2f40
links
openurl$$Topenurl_article
thumbnail$$Usyndetics_thumb_exl
backlink$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24937531$$D View this record in MEDLINE/PubMed
search
creatorcontrib
0Santovito, Donato
1De Nardis, Velia
2Marcantonio, Pamela
3Mandolini, Claudia
4Paganelli, Camilla
5Vitale, Elita
6Buttitta, Fiamma
7Bucci, Marco
8Mezzetti, Andrea
9Consoli, Agostino
10Cipollone, Francesco
title
0Plasma Exosome MicroRNA Profiling Unravels a New Potential Modulator of Adiponectin Pathway in Diabetes: Effect of Glycemic Control
1The journal of clinical endocrinology and metabolism
addtitleJ Clin Endocrinol Metab
descriptionContext: Type 2 diabetes is a chronic disease characterized by inadequate β-cell response to the progressive insulin resistance. MicroRNAs (miRNAs) are short, endogenous, noncoding RNAs representing a class of powerful gene expression modulators. Previous population studies observed a modulation of circulating miRNAs in diabetic patients; however, few data are presently available on miRNA modulation in diabetic patients naïve to pharmacological treatment as well as the effect of glycemic control on this. Objective: We aimed at studying circulating miRNA expression in diabetic patients naïve to treatment and at investigating the influence on this of glycemic control. Design: This was a case-control study. Participants: Eighteen treatment-naïve diabetic patients with poor metabolic control and 12 control patients participated in the study. Main Outcome Measures: Wide miRNA expression profiling was performed, and the expression of miRNAs found to be dysregulated was then validated by quantitative RT-PCR. Finally, algorithm-identified putative miRNA targets were evaluated by quantitative RT-PCR and ELISA. Results: In diabetic patients, microarray analysis showed that four miRNAs are increased, whereas 21 miRNAs are decreased. Quantitative RT-PCR validation confirmed the significant up-regulation of miR-326 (P = .004) and down-regulation of let-7a (P < .001) and let-7f (P = .003). Notably, an inverse negative correlation was found between circulating miR-326 and its putative target adiponectin (p = −0.479, P = .009). After 12 months of antidiabetic treatment, quantitative RT-PCR data analysis showed that miR-326 levels were unaffected, whereas the levels of let-7a and let-7f were significantly increased. Conclusions: Treatment-naïve, poorly controlled diabetic patients show a significant dysregulation of miRNAs involved in the regulation of the adiponectin pathway, a phenomenon that may be reversed, at least in part, by improved glycemic control.
subject
0Abridged Index Medicus
1Adiponectin - genetics
2Adiponectin - metabolism
3Aged
4Case-Control Studies
5Diabetes Mellitus, Type 2 - drug therapy
6Diabetes Mellitus, Type 2 - genetics
7Diabetes Mellitus, Type 2 - metabolism
8Exosomes - genetics
9Female
10Humans
11Hyperglycemia - drug therapy
12Hyperglycemia - genetics
13Hyperglycemia - metabolism
14Hypoglycemic Agents - therapeutic use
15Insulin Resistance - genetics
16Male
17MicroRNAs - blood
18MicroRNAs - genetics
19Middle Aged
20Transcriptome
issn
00021-972X
11945-7197
fulltextfalse
rsrctypearticle
creationdate2014
recordtypearticle
recordideNp1kD1vFDEQQC1ERC6Bjhq5pGCDP_eD7nRcAlISTohIdJbXOws-ee2L7SVczR9nVxcooqSakebNKx5Cryk5o4yS91tzxgjlBa8Ff4YWtBGyqGhTPUcLQhgtmop9P0YnKW0JoUJI_gIdM9HwSnK6QH82TqdB4_XvkMIA-MqaGL5eL_Emht4663_gGx_1L3AJa3wNd3gTMvhstcNXoRudziHi0ONlZ3fBg8nW443OP-_0Hk_rR6tbyJA-4HXfT9cZvXB7A4M1eBV8jsG9REe9dgle3c9TdHO-_rb6VFx-ufi8Wl4WlNc8F21F6o6RqjQ1aCGF6SsCDafQsLoUouVMaqkFFQRM3ZdQSlEZCaSruGxZL8gpenvw7mK4HSFlNdhkwDntIYxJUVkSWcuSsgl9d0CnGilF6NUu2kHHvaJEzdnV1qg5u5qzT_ibe_PYDtD9h_91ngD2wGds1tnOAbR1T1n54Ql8F0y0HnYRUlLbMEY_hXr86y9Ko59B
startdate201409
enddate201409
creator
0Santovito, Donato
1De Nardis, Velia
2Marcantonio, Pamela
3Mandolini, Claudia
4Paganelli, Camilla
5Vitale, Elita
6Buttitta, Fiamma
7Bucci, Marco
8Mezzetti, Andrea
9Consoli, Agostino
10Cipollone, Francesco
generalEndocrine Society
scope
0CGR
1CUY
2CVF
3ECM
4EIF
5NPM
6AAYXX
7CITATION
87X8
sort
creationdate201409
titlePlasma Exosome MicroRNA Profiling Unravels a New Potential Modulator of Adiponectin Pathway in Diabetes: Effect of Glycemic Control
authorSantovito, Donato ; De Nardis, Velia ; Marcantonio, Pamela ; Mandolini, Claudia ; Paganelli, Camilla ; Vitale, Elita ; Buttitta, Fiamma ; Bucci, Marco ; Mezzetti, Andrea ; Consoli, Agostino ; Cipollone, Francesco
facets
frbrtype5
frbrgroupidcdi_FETCH-LOGICAL-1383t-b708d2076c8ea454cf70e931e928644b325a5a4140ec8f6e6547c5e0d735b2f40
rsrctypearticles
prefilterarticles
languageeng
creationdate2014
topic
0Abridged Index Medicus
1Adiponectin - genetics
2Adiponectin - metabolism
3Aged
4Case-Control Studies
5Diabetes Mellitus, Type 2 - drug therapy
6Diabetes Mellitus, Type 2 - genetics
7Diabetes Mellitus, Type 2 - metabolism
8Exosomes - genetics
9Female
10Humans
11Hyperglycemia - drug therapy
12Hyperglycemia - genetics
13Hyperglycemia - metabolism
14Hypoglycemic Agents - therapeutic use
15Insulin Resistance - genetics
16Male
17MicroRNAs - blood
18MicroRNAs - genetics
19Middle Aged
20Transcriptome
toplevelpeer_reviewed
creatorcontrib
0Santovito, Donato
1De Nardis, Velia
2Marcantonio, Pamela
3Mandolini, Claudia
4Paganelli, Camilla
5Vitale, Elita
6Buttitta, Fiamma
7Bucci, Marco
8Mezzetti, Andrea
9Consoli, Agostino
10Cipollone, Francesco
collection
0Medline
1MEDLINE
2MEDLINE (Ovid)
3MEDLINE
4MEDLINE
5PubMed
6CrossRef
7MEDLINE - Academic
jtitleThe journal of clinical endocrinology and metabolism
delivery
delcategoryRemote Search Resource
fulltextno_fulltext
addata
au
0Santovito, Donato
1De Nardis, Velia
2Marcantonio, Pamela
3Mandolini, Claudia
4Paganelli, Camilla
5Vitale, Elita
6Buttitta, Fiamma
7Bucci, Marco
8Mezzetti, Andrea
9Consoli, Agostino
10Cipollone, Francesco
formatjournal
genrearticle
ristypeJOUR
atitlePlasma Exosome MicroRNA Profiling Unravels a New Potential Modulator of Adiponectin Pathway in Diabetes: Effect of Glycemic Control
jtitleThe journal of clinical endocrinology and metabolism
addtitleJ Clin Endocrinol Metab
date2014-09
risdate2014
volume99
issue9
spageE1681
epageE1685
pagesE1681-E1685
issn0021-972X
eissn1945-7197
notesThis work was partly supported by a research grant from the Italian Ministry of University and Scientific Research (reference COFIN MIUR 2009 Protocol Number 2009L4X28T_002).
abstractContext: Type 2 diabetes is a chronic disease characterized by inadequate β-cell response to the progressive insulin resistance. MicroRNAs (miRNAs) are short, endogenous, noncoding RNAs representing a class of powerful gene expression modulators. Previous population studies observed a modulation of circulating miRNAs in diabetic patients; however, few data are presently available on miRNA modulation in diabetic patients naïve to pharmacological treatment as well as the effect of glycemic control on this. Objective: We aimed at studying circulating miRNA expression in diabetic patients naïve to treatment and at investigating the influence on this of glycemic control. Design: This was a case-control study. Participants: Eighteen treatment-naïve diabetic patients with poor metabolic control and 12 control patients participated in the study. Main Outcome Measures: Wide miRNA expression profiling was performed, and the expression of miRNAs found to be dysregulated was then validated by quantitative RT-PCR. Finally, algorithm-identified putative miRNA targets were evaluated by quantitative RT-PCR and ELISA. Results: In diabetic patients, microarray analysis showed that four miRNAs are increased, whereas 21 miRNAs are decreased. Quantitative RT-PCR validation confirmed the significant up-regulation of miR-326 (P = .004) and down-regulation of let-7a (P < .001) and let-7f (P = .003). Notably, an inverse negative correlation was found between circulating miR-326 and its putative target adiponectin (p = −0.479, P = .009). After 12 months of antidiabetic treatment, quantitative RT-PCR data analysis showed that miR-326 levels were unaffected, whereas the levels of let-7a and let-7f were significantly increased. Conclusions: Treatment-naïve, poorly controlled diabetic patients show a significant dysregulation of miRNAs involved in the regulation of the adiponectin pathway, a phenomenon that may be reversed, at least in part, by improved glycemic control.
copUnited States
pubEndocrine Society
pmid24937531
doi10.1210/jc.2013-3843
oafree_for_read