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Overexpression of a wheat phospholipase D gene, TaPLDα, enhances tolerance to drought and osmotic stress in Arabidopsis thaliana

Phospholipase D (PLD) is crucial for plant responses to stress and signal transduction, however, the regulatory mechanism of PLD in abiotic stress is not completely understood; especially, in crops. In this study, we isolated a gene, TaPLDα, from common wheat (Triticum aestivum L.). Analysis of the... Full description

Journal Title: Planta 2014, Vol.240 (1), p.103-115
Main Author: Wang, Junbin
Other Authors: Ding, Bo , Guo, Yaolin , Li, Ming , Chen, Shuaijun , Huang, Guozhong , Xie, Xiaodong
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: Berlin/Heidelberg: Springer-Verlag
ID: ISSN: 0032-0935
Link: https://www.ncbi.nlm.nih.gov/pubmed/24705986
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title: Overexpression of a wheat phospholipase D gene, TaPLDα, enhances tolerance to drought and osmotic stress in Arabidopsis thaliana
format: Article
creator:
  • Wang, Junbin
  • Ding, Bo
  • Guo, Yaolin
  • Li, Ming
  • Chen, Shuaijun
  • Huang, Guozhong
  • Xie, Xiaodong
subjects:
  • Agriculture
  • Amino Acid Sequence
  • Arabidopsis - enzymology
  • Arabidopsis - genetics
  • Arabidopsis - growth & development
  • Arabidopsis - physiology
  • Arabidopsis thaliana
  • Biomedical and Life Sciences
  • Dehydration
  • Drought
  • Droughts
  • Ecology
  • Ethanol
  • Forestry
  • Gene Expression
  • Gene Expression Regulation, Plant
  • Germination
  • Life Sciences
  • Molecular Sequence Data
  • Original Article
  • Osmotic Pressure
  • Phosphatidic acids
  • Phospholipase D - genetics
  • Phospholipase D - metabolism
  • Phylogeny
  • Plant cells
  • Plant Leaves - enzymology
  • Plant Leaves - genetics
  • Plant Leaves - growth & development
  • Plant Leaves - physiology
  • Plant Proteins - genetics
  • Plant Proteins - metabolism
  • Plant Roots - enzymology
  • Plant Roots - genetics
  • Plant Roots - growth & development
  • Plant Roots - physiology
  • Plant Sciences
  • Plants
  • Plants, Genetically Modified
  • Seedlings - enzymology
  • Seedlings - genetics
  • Seedlings - growth & development
  • Seedlings - physiology
  • Seeds - enzymology
  • Seeds - genetics
  • Seeds - growth & development
  • Seeds - physiology
  • Sequence Analysis, DNA
  • Signal Transduction
  • Stress tolerance
  • Stress, Physiological
  • Transgenes
  • Transgenic plants
  • Triticum - enzymology
  • Triticum - genetics
  • Triticum aestivum
  • Water loss
  • Wheat
ispartof: Planta, 2014, Vol.240 (1), p.103-115
description: Phospholipase D (PLD) is crucial for plant responses to stress and signal transduction, however, the regulatory mechanism of PLD in abiotic stress is not completely understood; especially, in crops. In this study, we isolated a gene, TaPLDα, from common wheat (Triticum aestivum L.). Analysis of the amino acid sequence of TaPLDα revealed a highly conserved C2 domain and two characteristic HKD motifs, which is similar to other known PLD family genes. Further characterization revealed that TaPLDα expressed differentially in various organs, such as roots, stems, leaves and spikelets of wheat. After treatment with abscisic acid (ABA), methyl jasmonate, dehydration, polyethylene glycol and NaCl, the expression of TaPLDα was up-regulated in shoots. Subsequently, we generated TaPLDα-overexpressing transgenic Arabidopsis lines under the control of the dexamethasone-inducible 35S promoter. The overexpression of TaPLDα in Arabidopsis resulted in significantly enhanced tolerance to drought, as shown by reduced chlorosis and leaf water loss, higher relative water content and lower relative electrolyte leakage than the wild type. Moreover, the TaPLDα-overexpressing plants exhibited longer roots in response to mannitol treatment. In addition, the seeds of TaPLDα-overexpressing plants showed hypersensitivity to ABA and osmotic stress. Under dehydration, the expression of several stress-related genes, RD29A, RD29B, KIN1 and RAB18, was up-regulated to a higher level in TaPLDα-overexpressing plants than in wild type. Taken together, our results indicated that TaPLDα can enhance tolerance to drought and osmotic stress in Arabidopsis and represents a potential candidate gene to enhance stress tolerance in crops.
language: eng
source:
identifier: ISSN: 0032-0935
fulltext: no_fulltext
issn:
  • 0032-0935
  • 1432-2048
url: Link


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titleOverexpression of a wheat phospholipase D gene, TaPLDα, enhances tolerance to drought and osmotic stress in Arabidopsis thaliana
creatorWang, Junbin ; Ding, Bo ; Guo, Yaolin ; Li, Ming ; Chen, Shuaijun ; Huang, Guozhong ; Xie, Xiaodong
creatorcontribWang, Junbin ; Ding, Bo ; Guo, Yaolin ; Li, Ming ; Chen, Shuaijun ; Huang, Guozhong ; Xie, Xiaodong
descriptionPhospholipase D (PLD) is crucial for plant responses to stress and signal transduction, however, the regulatory mechanism of PLD in abiotic stress is not completely understood; especially, in crops. In this study, we isolated a gene, TaPLDα, from common wheat (Triticum aestivum L.). Analysis of the amino acid sequence of TaPLDα revealed a highly conserved C2 domain and two characteristic HKD motifs, which is similar to other known PLD family genes. Further characterization revealed that TaPLDα expressed differentially in various organs, such as roots, stems, leaves and spikelets of wheat. After treatment with abscisic acid (ABA), methyl jasmonate, dehydration, polyethylene glycol and NaCl, the expression of TaPLDα was up-regulated in shoots. Subsequently, we generated TaPLDα-overexpressing transgenic Arabidopsis lines under the control of the dexamethasone-inducible 35S promoter. The overexpression of TaPLDα in Arabidopsis resulted in significantly enhanced tolerance to drought, as shown by reduced chlorosis and leaf water loss, higher relative water content and lower relative electrolyte leakage than the wild type. Moreover, the TaPLDα-overexpressing plants exhibited longer roots in response to mannitol treatment. In addition, the seeds of TaPLDα-overexpressing plants showed hypersensitivity to ABA and osmotic stress. Under dehydration, the expression of several stress-related genes, RD29A, RD29B, KIN1 and RAB18, was up-regulated to a higher level in TaPLDα-overexpressing plants than in wild type. Taken together, our results indicated that TaPLDα can enhance tolerance to drought and osmotic stress in Arabidopsis and represents a potential candidate gene to enhance stress tolerance in crops.
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subjectAgriculture ; Amino Acid Sequence ; Arabidopsis - enzymology ; Arabidopsis - genetics ; Arabidopsis - growth & development ; Arabidopsis - physiology ; Arabidopsis thaliana ; Biomedical and Life Sciences ; Dehydration ; Drought ; Droughts ; Ecology ; Ethanol ; Forestry ; Gene Expression ; Gene Expression Regulation, Plant ; Germination ; Life Sciences ; Molecular Sequence Data ; Original Article ; Osmotic Pressure ; Phosphatidic acids ; Phospholipase D - genetics ; Phospholipase D - metabolism ; Phylogeny ; Plant cells ; Plant Leaves - enzymology ; Plant Leaves - genetics ; Plant Leaves - growth & development ; Plant Leaves - physiology ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Roots - enzymology ; Plant Roots - genetics ; Plant Roots - growth & development ; Plant Roots - physiology ; Plant Sciences ; Plants ; Plants, Genetically Modified ; Seedlings - enzymology ; Seedlings - genetics ; Seedlings - growth & development ; Seedlings - physiology ; Seeds - enzymology ; Seeds - genetics ; Seeds - growth & development ; Seeds - physiology ; Sequence Analysis, DNA ; Signal Transduction ; Stress tolerance ; Stress, Physiological ; Transgenes ; Transgenic plants ; Triticum - enzymology ; Triticum - genetics ; Triticum aestivum ; Water loss ; Wheat
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descriptionPhospholipase D (PLD) is crucial for plant responses to stress and signal transduction, however, the regulatory mechanism of PLD in abiotic stress is not completely understood; especially, in crops. In this study, we isolated a gene, TaPLDα, from common wheat (Triticum aestivum L.). Analysis of the amino acid sequence of TaPLDα revealed a highly conserved C2 domain and two characteristic HKD motifs, which is similar to other known PLD family genes. Further characterization revealed that TaPLDα expressed differentially in various organs, such as roots, stems, leaves and spikelets of wheat. After treatment with abscisic acid (ABA), methyl jasmonate, dehydration, polyethylene glycol and NaCl, the expression of TaPLDα was up-regulated in shoots. Subsequently, we generated TaPLDα-overexpressing transgenic Arabidopsis lines under the control of the dexamethasone-inducible 35S promoter. The overexpression of TaPLDα in Arabidopsis resulted in significantly enhanced tolerance to drought, as shown by reduced chlorosis and leaf water loss, higher relative water content and lower relative electrolyte leakage than the wild type. Moreover, the TaPLDα-overexpressing plants exhibited longer roots in response to mannitol treatment. In addition, the seeds of TaPLDα-overexpressing plants showed hypersensitivity to ABA and osmotic stress. Under dehydration, the expression of several stress-related genes, RD29A, RD29B, KIN1 and RAB18, was up-regulated to a higher level in TaPLDα-overexpressing plants than in wild type. Taken together, our results indicated that TaPLDα can enhance tolerance to drought and osmotic stress in Arabidopsis and represents a potential candidate gene to enhance stress tolerance in crops.
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1Amino Acid Sequence
2Arabidopsis - enzymology
3Arabidopsis - genetics
4Arabidopsis - growth & development
5Arabidopsis - physiology
6Arabidopsis thaliana
7Biomedical and Life Sciences
8Dehydration
9Drought
10Droughts
11Ecology
12Ethanol
13Forestry
14Gene Expression
15Gene Expression Regulation, Plant
16Germination
17Life Sciences
18Molecular Sequence Data
19Original Article
20Osmotic Pressure
21Phosphatidic acids
22Phospholipase D - genetics
23Phospholipase D - metabolism
24Phylogeny
25Plant cells
26Plant Leaves - enzymology
27Plant Leaves - genetics
28Plant Leaves - growth & development
29Plant Leaves - physiology
30Plant Proteins - genetics
31Plant Proteins - metabolism
32Plant Roots - enzymology
33Plant Roots - genetics
34Plant Roots - growth & development
35Plant Roots - physiology
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titleOverexpression of a wheat phospholipase D gene, TaPLDα, enhances tolerance to drought and osmotic stress in Arabidopsis thaliana
authorWang, Junbin ; Ding, Bo ; Guo, Yaolin ; Li, Ming ; Chen, Shuaijun ; Huang, Guozhong ; Xie, Xiaodong
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abstractPhospholipase D (PLD) is crucial for plant responses to stress and signal transduction, however, the regulatory mechanism of PLD in abiotic stress is not completely understood; especially, in crops. In this study, we isolated a gene, TaPLDα, from common wheat (Triticum aestivum L.). Analysis of the amino acid sequence of TaPLDα revealed a highly conserved C2 domain and two characteristic HKD motifs, which is similar to other known PLD family genes. Further characterization revealed that TaPLDα expressed differentially in various organs, such as roots, stems, leaves and spikelets of wheat. After treatment with abscisic acid (ABA), methyl jasmonate, dehydration, polyethylene glycol and NaCl, the expression of TaPLDα was up-regulated in shoots. Subsequently, we generated TaPLDα-overexpressing transgenic Arabidopsis lines under the control of the dexamethasone-inducible 35S promoter. The overexpression of TaPLDα in Arabidopsis resulted in significantly enhanced tolerance to drought, as shown by reduced chlorosis and leaf water loss, higher relative water content and lower relative electrolyte leakage than the wild type. Moreover, the TaPLDα-overexpressing plants exhibited longer roots in response to mannitol treatment. In addition, the seeds of TaPLDα-overexpressing plants showed hypersensitivity to ABA and osmotic stress. Under dehydration, the expression of several stress-related genes, RD29A, RD29B, KIN1 and RAB18, was up-regulated to a higher level in TaPLDα-overexpressing plants than in wild type. Taken together, our results indicated that TaPLDα can enhance tolerance to drought and osmotic stress in Arabidopsis and represents a potential candidate gene to enhance stress tolerance in crops.
copBerlin/Heidelberg
pubSpringer-Verlag
pmid24705986
doi10.1007/s00425-014-2066-6