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Maternal low-protein diet affects myostatin signaling and protein synthesis in skeletal muscle of offspring piglets at weaning stage

Purpose We tested the hypothesis that maternal low-protein (LP) diet during gestation and lactation can program myostatin (MSTN) signaling and protein synthesis in skeletal muscle of offspring at weaning stage (35 days). Methods Fourteen Meishan sows were fed either LP or standard-protein diets thro... Full description

Journal Title: European journal of nutrition 2014, Vol.54 (6), p.971-979
Main Author: Liu, Xiujuan
Other Authors: Pan, Shifeng , Li, Xiao , Sun, Qinwei , Yang, Xiaojing , Zhao, Ruqian
Format: Electronic Article Electronic Article
Language: English
Subjects:
Amino Acids - blood
Amino Acids, Branched-Chain - blood
Animal Nutritional Physiological Phenomena
Animals
Branched chain amino acids
Chemistry
Chemistry and Materials Science
Diet, Protein-Restricted - adverse effects
Eukaryotic Initiation Factor-4E - analysis
Female
Genetic translation
Lactation
Male
Maternal Nutritional Physiological Phenomena
Muscle Proteins - biosynthesis
Muscle, Skeletal - growth & development
Muscles
Myostatin - metabolism
Nutrition
Organ Size
Original Contribution
Physiological aspects
Pregnancy
Prenatal Exposure Delayed Effects
Protein biosynthesis
Proteins
Ribosomal Protein S6 Kinases, 70-kDa - analysis
RNA
Signal Transduction
Sus scrofa
Swine
Weaning
Publisher: Berlin/Heidelberg: Springer Berlin Heidelberg
ID: ISSN: 1436-6207
Link: https://www.ncbi.nlm.nih.gov/pubmed/25266448
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recordid: cdi_proquest_miscellaneous_1705734615
title: Maternal low-protein diet affects myostatin signaling and protein synthesis in skeletal muscle of offspring piglets at weaning stage
format: Article
creator:
  • Liu, Xiujuan
  • Pan, Shifeng
  • Li, Xiao
  • Sun, Qinwei
  • Yang, Xiaojing
  • Zhao, Ruqian
subjects:
  • Amino Acids - blood
  • Amino Acids, Branched-Chain - blood
  • Animal Nutritional Physiological Phenomena
  • Animals
  • Branched chain amino acids
  • Chemistry
  • Chemistry and Materials Science
  • Diet, Protein-Restricted - adverse effects
  • Eukaryotic Initiation Factor-4E - analysis
  • Female
  • Genetic translation
  • Lactation
  • Male
  • Maternal Nutritional Physiological Phenomena
  • Muscle Proteins - biosynthesis
  • Muscle, Skeletal - growth & development
  • Muscles
  • Myostatin - metabolism
  • Nutrition
  • Organ Size
  • Original Contribution
  • Physiological aspects
  • Pregnancy
  • Prenatal Exposure Delayed Effects
  • Protein biosynthesis
  • Proteins
  • Ribosomal Protein S6 Kinases, 70-kDa - analysis
  • RNA
  • Signal Transduction
  • Sus scrofa
  • Swine
  • Weaning
ispartof: European journal of nutrition, 2014, Vol.54 (6), p.971-979
description: Purpose We tested the hypothesis that maternal low-protein (LP) diet during gestation and lactation can program myostatin (MSTN) signaling and protein synthesis in skeletal muscle of offspring at weaning stage (35 days). Methods Fourteen Meishan sows were fed either LP or standard-protein diets throughout gestation and lactation, male offspring piglets were killed at weaning stage and longissimus dorsi (LD) muscles were taken. The cross-sectional areas (CSA) of LD muscles were measured by hematoxylin and eosin staining. The levels of free amino acids in plasma were measured by amino acid auto-analyzer. Proteins and mRNA were determined by Western blot and RT-qPCR, respectively. Results Body weight, LD muscle weight and the myofiber CSA were significantly decreased ( P  
language: eng
source:
identifier: ISSN: 1436-6207
fulltext: no_fulltext
issn:
  • 1436-6207
  • 1436-6215
url: Link


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titleMaternal low-protein diet affects myostatin signaling and protein synthesis in skeletal muscle of offspring piglets at weaning stage
creatorLiu, Xiujuan ; Pan, Shifeng ; Li, Xiao ; Sun, Qinwei ; Yang, Xiaojing ; Zhao, Ruqian
creatorcontribLiu, Xiujuan ; Pan, Shifeng ; Li, Xiao ; Sun, Qinwei ; Yang, Xiaojing ; Zhao, Ruqian
descriptionPurpose We tested the hypothesis that maternal low-protein (LP) diet during gestation and lactation can program myostatin (MSTN) signaling and protein synthesis in skeletal muscle of offspring at weaning stage (35 days). Methods Fourteen Meishan sows were fed either LP or standard-protein diets throughout gestation and lactation, male offspring piglets were killed at weaning stage and longissimus dorsi (LD) muscles were taken. The cross-sectional areas (CSA) of LD muscles were measured by hematoxylin and eosin staining. The levels of free amino acids in plasma were measured by amino acid auto-analyzer. Proteins and mRNA were determined by Western blot and RT-qPCR, respectively. Results Body weight, LD muscle weight and the myofiber CSA were significantly decreased ( P  < 0.05) in LP piglets; meanwhile, the concentration of branched-chain amino acids was also significantly decreased ( P  < 0.001). MSTN protein content tended to be higher ( P  = 0.098) in LP piglets, while the expression of MSTN receptors, activin type II receptor-beta and transforming growth factor type-beta type I receptor kinase, was significantly up-regulated ( P  < 0.05). Furthermore, p38 mitogen-activated protein kinase, the downstream signaling factor of MSTN, was also enhanced significantly ( P  < 0.05). In addition, key factors of translation initiation, phosphorylated eukaryotic initiation factor 4E and the 70 kDa ribosomal protein S6 kinase, were significantly decreased ( P  < 0.05) in LP piglets. Conclusions Our results suggest that maternal LP diet during gestation and lactation affects MSTN signaling and protein synthesis in skeletal muscle of offspring at weaning stage.
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2DOI: 10.1007/s00394-014-0773-1
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languageeng
publisherBerlin/Heidelberg: Springer Berlin Heidelberg
subjectAmino Acids - blood ; Amino Acids, Branched-Chain - blood ; Animal Nutritional Physiological Phenomena ; Animals ; Branched chain amino acids ; Chemistry ; Chemistry and Materials Science ; Diet, Protein-Restricted - adverse effects ; Eukaryotic Initiation Factor-4E - analysis ; Female ; Genetic translation ; Lactation ; Male ; Maternal Nutritional Physiological Phenomena ; Muscle Proteins - biosynthesis ; Muscle, Skeletal - growth & development ; Muscles ; Myostatin - metabolism ; Nutrition ; Organ Size ; Original Contribution ; Physiological aspects ; Pregnancy ; Prenatal Exposure Delayed Effects ; Protein biosynthesis ; Proteins ; Ribosomal Protein S6 Kinases, 70-kDa - analysis ; RNA ; Signal Transduction ; Sus scrofa ; Swine ; Weaning
ispartofEuropean journal of nutrition, 2014, Vol.54 (6), p.971-979
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3Sun, Qinwei
4Yang, Xiaojing
5Zhao, Ruqian
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1European journal of nutrition
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descriptionPurpose We tested the hypothesis that maternal low-protein (LP) diet during gestation and lactation can program myostatin (MSTN) signaling and protein synthesis in skeletal muscle of offspring at weaning stage (35 days). Methods Fourteen Meishan sows were fed either LP or standard-protein diets throughout gestation and lactation, male offspring piglets were killed at weaning stage and longissimus dorsi (LD) muscles were taken. The cross-sectional areas (CSA) of LD muscles were measured by hematoxylin and eosin staining. The levels of free amino acids in plasma were measured by amino acid auto-analyzer. Proteins and mRNA were determined by Western blot and RT-qPCR, respectively. Results Body weight, LD muscle weight and the myofiber CSA were significantly decreased ( P  < 0.05) in LP piglets; meanwhile, the concentration of branched-chain amino acids was also significantly decreased ( P  < 0.001). MSTN protein content tended to be higher ( P  = 0.098) in LP piglets, while the expression of MSTN receptors, activin type II receptor-beta and transforming growth factor type-beta type I receptor kinase, was significantly up-regulated ( P  < 0.05). Furthermore, p38 mitogen-activated protein kinase, the downstream signaling factor of MSTN, was also enhanced significantly ( P  < 0.05). In addition, key factors of translation initiation, phosphorylated eukaryotic initiation factor 4E and the 70 kDa ribosomal protein S6 kinase, were significantly decreased ( P  < 0.05) in LP piglets. Conclusions Our results suggest that maternal LP diet during gestation and lactation affects MSTN signaling and protein synthesis in skeletal muscle of offspring at weaning stage.
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1Amino Acids, Branched-Chain - blood
2Animal Nutritional Physiological Phenomena
3Animals
4Branched chain amino acids
5Chemistry
6Chemistry and Materials Science
7Diet, Protein-Restricted - adverse effects
8Eukaryotic Initiation Factor-4E - analysis
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10Genetic translation
11Lactation
12Male
13Maternal Nutritional Physiological Phenomena
14Muscle Proteins - biosynthesis
15Muscle, Skeletal - growth & development
16Muscles
17Myostatin - metabolism
18Nutrition
19Organ Size
20Original Contribution
21Physiological aspects
22Pregnancy
23Prenatal Exposure Delayed Effects
24Protein biosynthesis
25Proteins
26Ribosomal Protein S6 Kinases, 70-kDa - analysis
27RNA
28Signal Transduction
29Sus scrofa
30Swine
31Weaning
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titleMaternal low-protein diet affects myostatin signaling and protein synthesis in skeletal muscle of offspring piglets at weaning stage
authorLiu, Xiujuan ; Pan, Shifeng ; Li, Xiao ; Sun, Qinwei ; Yang, Xiaojing ; Zhao, Ruqian
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28Signal Transduction
29Sus scrofa
30Swine
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atitleMaternal low-protein diet affects myostatin signaling and protein synthesis in skeletal muscle of offspring piglets at weaning stage
jtitleEuropean journal of nutrition
stitleEur J Nutr
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date2014-09-30
risdate2014
volume54
issue6
spage971
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pages971-979
issn1436-6207
eissn1436-6215
abstractPurpose We tested the hypothesis that maternal low-protein (LP) diet during gestation and lactation can program myostatin (MSTN) signaling and protein synthesis in skeletal muscle of offspring at weaning stage (35 days). Methods Fourteen Meishan sows were fed either LP or standard-protein diets throughout gestation and lactation, male offspring piglets were killed at weaning stage and longissimus dorsi (LD) muscles were taken. The cross-sectional areas (CSA) of LD muscles were measured by hematoxylin and eosin staining. The levels of free amino acids in plasma were measured by amino acid auto-analyzer. Proteins and mRNA were determined by Western blot and RT-qPCR, respectively. Results Body weight, LD muscle weight and the myofiber CSA were significantly decreased ( P  < 0.05) in LP piglets; meanwhile, the concentration of branched-chain amino acids was also significantly decreased ( P  < 0.001). MSTN protein content tended to be higher ( P  = 0.098) in LP piglets, while the expression of MSTN receptors, activin type II receptor-beta and transforming growth factor type-beta type I receptor kinase, was significantly up-regulated ( P  < 0.05). Furthermore, p38 mitogen-activated protein kinase, the downstream signaling factor of MSTN, was also enhanced significantly ( P  < 0.05). In addition, key factors of translation initiation, phosphorylated eukaryotic initiation factor 4E and the 70 kDa ribosomal protein S6 kinase, were significantly decreased ( P  < 0.05) in LP piglets. Conclusions Our results suggest that maternal LP diet during gestation and lactation affects MSTN signaling and protein synthesis in skeletal muscle of offspring at weaning stage.
copBerlin/Heidelberg
pubSpringer Berlin Heidelberg
pmid25266448
doi10.1007/s00394-014-0773-1