schliessen

Filtern

 

Bibliotheken

Maternal low-protein diet affects myostatin signaling and protein synthesis in skeletal muscle of offspring piglets at weaning stage

Purpose We tested the hypothesis that maternal low-protein (LP) diet during gestation and lactation can program myostatin (MSTN) signaling and protein synthesis in skeletal muscle of offspring at weaning stage (35 days). Methods Fourteen Meishan sows were fed either LP or standard-protein diets thro... Full description

Journal Title: European journal of nutrition 2014, Vol.54 (6), p.971-979
Main Author: Liu, Xiujuan
Other Authors: Pan, Shifeng , Li, Xiao , Sun, Qinwei , Yang, Xiaojing , Zhao, Ruqian
Format: Electronic Article Electronic Article
Language: English
Subjects:
RNA
Publisher: Berlin/Heidelberg: Springer Berlin Heidelberg
ID: ISSN: 1436-6207
Link: https://www.ncbi.nlm.nih.gov/pubmed/25266448
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: cdi_proquest_miscellaneous_1705734615
title: Maternal low-protein diet affects myostatin signaling and protein synthesis in skeletal muscle of offspring piglets at weaning stage
format: Article
creator:
  • Liu, Xiujuan
  • Pan, Shifeng
  • Li, Xiao
  • Sun, Qinwei
  • Yang, Xiaojing
  • Zhao, Ruqian
subjects:
  • Amino Acids - blood
  • Amino Acids, Branched-Chain - blood
  • Animal Nutritional Physiological Phenomena
  • Animals
  • Branched chain amino acids
  • Chemistry
  • Chemistry and Materials Science
  • Diet, Protein-Restricted - adverse effects
  • Eukaryotic Initiation Factor-4E - analysis
  • Female
  • Genetic translation
  • Lactation
  • Male
  • Maternal Nutritional Physiological Phenomena
  • Muscle Proteins - biosynthesis
  • Muscle, Skeletal - growth & development
  • Muscles
  • Myostatin - metabolism
  • Nutrition
  • Organ Size
  • Original Contribution
  • Physiological aspects
  • Pregnancy
  • Prenatal Exposure Delayed Effects
  • Protein biosynthesis
  • Proteins
  • Ribosomal Protein S6 Kinases, 70-kDa - analysis
  • RNA
  • Signal Transduction
  • Sus scrofa
  • Swine
  • Weaning
ispartof: European journal of nutrition, 2014, Vol.54 (6), p.971-979
description: Purpose We tested the hypothesis that maternal low-protein (LP) diet during gestation and lactation can program myostatin (MSTN) signaling and protein synthesis in skeletal muscle of offspring at weaning stage (35 days). Methods Fourteen Meishan sows were fed either LP or standard-protein diets throughout gestation and lactation, male offspring piglets were killed at weaning stage and longissimus dorsi (LD) muscles were taken. The cross-sectional areas (CSA) of LD muscles were measured by hematoxylin and eosin staining. The levels of free amino acids in plasma were measured by amino acid auto-analyzer. Proteins and mRNA were determined by Western blot and RT-qPCR, respectively. Results Body weight, LD muscle weight and the myofiber CSA were significantly decreased ( P  
language: eng
source:
identifier: ISSN: 1436-6207
fulltext: no_fulltext
issn:
  • 1436-6207
  • 1436-6215
url: Link


@attributes
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
RANK2.4508011
LOCALfalse
PrimoNMBib
record
control
sourceidgale_proqu
recordidTN_cdi_proquest_miscellaneous_1705734615
sourceformatXML
sourcesystemPC
galeidA425928746
sourcerecordidA425928746
originalsourceidFETCH-LOGICAL-c430t-89456f3a7d856cb595d76a4854c39e4b32203bf68737ebef19dcba818683339b0
addsrcrecordideNp9kVFv1iAUhonRuPnpD_DGkHjjTScUCvRyWeY0mfFGrwmlh8ps4bPQLN-9P1y6bstc1JQGODzvm5PzIvSakhNKiHyfCGEtrwgtv5Ssok_QMeVMVKKmzdP7M5FH6EVKV4SQmgn6HB3VTS0E5-oY_fpsMszBjHiM19V-jhl8wL2HjI1zYHPC0yGmbHIpJz8U0ocBm9DjOzgdQv4OySe8Xn7ACLnYTUuyI-DoynJpP6-qvR_KY8Im42swYS0V5wFeomfOjAle3e479O3D-dezj9Xll4tPZ6eXleWM5Eq1vBGOGdmrRtiuaZteCsNVwy1rgXesrgnrnFCSSejA0ba3nVFUCcUYazuyQ-8239L6zwVS1pNPFsbRBIhL0lSSRjIuaFPQt4_Qq7isc7qheMuoUg-owYygfXAxz8aupvqU101bK8lFoU7-QpWvh8nbGMD5Uv9DQDeBnWNKMzhd5jeZ-aAp0Wvyektel-T1mnwp7tCb24aXboL-XnEXdQHkI1Pr11hjKN348b_W9abcYoT5wSz-KfoNJsnKaA
sourcetypeAggregation Database
isCDItrue
recordtypearticle
pqid1704931885
display
typearticle
titleMaternal low-protein diet affects myostatin signaling and protein synthesis in skeletal muscle of offspring piglets at weaning stage
creatorLiu, Xiujuan ; Pan, Shifeng ; Li, Xiao ; Sun, Qinwei ; Yang, Xiaojing ; Zhao, Ruqian
creatorcontribLiu, Xiujuan ; Pan, Shifeng ; Li, Xiao ; Sun, Qinwei ; Yang, Xiaojing ; Zhao, Ruqian
descriptionPurpose We tested the hypothesis that maternal low-protein (LP) diet during gestation and lactation can program myostatin (MSTN) signaling and protein synthesis in skeletal muscle of offspring at weaning stage (35 days). Methods Fourteen Meishan sows were fed either LP or standard-protein diets throughout gestation and lactation, male offspring piglets were killed at weaning stage and longissimus dorsi (LD) muscles were taken. The cross-sectional areas (CSA) of LD muscles were measured by hematoxylin and eosin staining. The levels of free amino acids in plasma were measured by amino acid auto-analyzer. Proteins and mRNA were determined by Western blot and RT-qPCR, respectively. Results Body weight, LD muscle weight and the myofiber CSA were significantly decreased ( P  < 0.05) in LP piglets; meanwhile, the concentration of branched-chain amino acids was also significantly decreased ( P  < 0.001). MSTN protein content tended to be higher ( P  = 0.098) in LP piglets, while the expression of MSTN receptors, activin type II receptor-beta and transforming growth factor type-beta type I receptor kinase, was significantly up-regulated ( P  < 0.05). Furthermore, p38 mitogen-activated protein kinase, the downstream signaling factor of MSTN, was also enhanced significantly ( P  < 0.05). In addition, key factors of translation initiation, phosphorylated eukaryotic initiation factor 4E and the 70 kDa ribosomal protein S6 kinase, were significantly decreased ( P  < 0.05) in LP piglets. Conclusions Our results suggest that maternal LP diet during gestation and lactation affects MSTN signaling and protein synthesis in skeletal muscle of offspring at weaning stage.
identifier
0ISSN: 1436-6207
1EISSN: 1436-6215
2DOI: 10.1007/s00394-014-0773-1
3PMID: 25266448
languageeng
publisherBerlin/Heidelberg: Springer Berlin Heidelberg
subjectAmino Acids - blood ; Amino Acids, Branched-Chain - blood ; Animal Nutritional Physiological Phenomena ; Animals ; Branched chain amino acids ; Chemistry ; Chemistry and Materials Science ; Diet, Protein-Restricted - adverse effects ; Eukaryotic Initiation Factor-4E - analysis ; Female ; Genetic translation ; Lactation ; Male ; Maternal Nutritional Physiological Phenomena ; Muscle Proteins - biosynthesis ; Muscle, Skeletal - growth & development ; Muscles ; Myostatin - metabolism ; Nutrition ; Organ Size ; Original Contribution ; Physiological aspects ; Pregnancy ; Prenatal Exposure Delayed Effects ; Protein biosynthesis ; Proteins ; Ribosomal Protein S6 Kinases, 70-kDa - analysis ; RNA ; Signal Transduction ; Sus scrofa ; Swine ; Weaning
ispartofEuropean journal of nutrition, 2014, Vol.54 (6), p.971-979
rights
0Springer-Verlag Berlin Heidelberg 2014
1COPYRIGHT 2015 Springer
2Springer-Verlag Berlin Heidelberg 2015
lds50peer_reviewed
citedbyFETCH-LOGICAL-c430t-89456f3a7d856cb595d76a4854c39e4b32203bf68737ebef19dcba818683339b0
citesFETCH-LOGICAL-c430t-89456f3a7d856cb595d76a4854c39e4b32203bf68737ebef19dcba818683339b0
links
openurl$$Topenurl_article
thumbnail$$Usyndetics_thumb_exl
backlink$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25266448$$D View this record in MEDLINE/PubMed
search
creatorcontrib
0Liu, Xiujuan
1Pan, Shifeng
2Li, Xiao
3Sun, Qinwei
4Yang, Xiaojing
5Zhao, Ruqian
title
0Maternal low-protein diet affects myostatin signaling and protein synthesis in skeletal muscle of offspring piglets at weaning stage
1European journal of nutrition
addtitle
0Eur J Nutr
1Eur J Nutr
descriptionPurpose We tested the hypothesis that maternal low-protein (LP) diet during gestation and lactation can program myostatin (MSTN) signaling and protein synthesis in skeletal muscle of offspring at weaning stage (35 days). Methods Fourteen Meishan sows were fed either LP or standard-protein diets throughout gestation and lactation, male offspring piglets were killed at weaning stage and longissimus dorsi (LD) muscles were taken. The cross-sectional areas (CSA) of LD muscles were measured by hematoxylin and eosin staining. The levels of free amino acids in plasma were measured by amino acid auto-analyzer. Proteins and mRNA were determined by Western blot and RT-qPCR, respectively. Results Body weight, LD muscle weight and the myofiber CSA were significantly decreased ( P  < 0.05) in LP piglets; meanwhile, the concentration of branched-chain amino acids was also significantly decreased ( P  < 0.001). MSTN protein content tended to be higher ( P  = 0.098) in LP piglets, while the expression of MSTN receptors, activin type II receptor-beta and transforming growth factor type-beta type I receptor kinase, was significantly up-regulated ( P  < 0.05). Furthermore, p38 mitogen-activated protein kinase, the downstream signaling factor of MSTN, was also enhanced significantly ( P  < 0.05). In addition, key factors of translation initiation, phosphorylated eukaryotic initiation factor 4E and the 70 kDa ribosomal protein S6 kinase, were significantly decreased ( P  < 0.05) in LP piglets. Conclusions Our results suggest that maternal LP diet during gestation and lactation affects MSTN signaling and protein synthesis in skeletal muscle of offspring at weaning stage.
subject
0Amino Acids - blood
1Amino Acids, Branched-Chain - blood
2Animal Nutritional Physiological Phenomena
3Animals
4Branched chain amino acids
5Chemistry
6Chemistry and Materials Science
7Diet, Protein-Restricted - adverse effects
8Eukaryotic Initiation Factor-4E - analysis
9Female
10Genetic translation
11Lactation
12Male
13Maternal Nutritional Physiological Phenomena
14Muscle Proteins - biosynthesis
15Muscle, Skeletal - growth & development
16Muscles
17Myostatin - metabolism
18Nutrition
19Organ Size
20Original Contribution
21Physiological aspects
22Pregnancy
23Prenatal Exposure Delayed Effects
24Protein biosynthesis
25Proteins
26Ribosomal Protein S6 Kinases, 70-kDa - analysis
27RNA
28Signal Transduction
29Sus scrofa
30Swine
31Weaning
issn
01436-6207
11436-6215
fulltextfalse
rsrctypearticle
creationdate2014
recordtypearticle
recordideNp9kVFv1iAUhonRuPnpD_DGkHjjTScUCvRyWeY0mfFGrwmlh8ps4bPQLN-9P1y6bstc1JQGODzvm5PzIvSakhNKiHyfCGEtrwgtv5Ssok_QMeVMVKKmzdP7M5FH6EVKV4SQmgn6HB3VTS0E5-oY_fpsMszBjHiM19V-jhl8wL2HjI1zYHPC0yGmbHIpJz8U0ocBm9DjOzgdQv4OySe8Xn7ACLnYTUuyI-DoynJpP6-qvR_KY8Im42swYS0V5wFeomfOjAle3e479O3D-dezj9Xll4tPZ6eXleWM5Eq1vBGOGdmrRtiuaZteCsNVwy1rgXesrgnrnFCSSejA0ba3nVFUCcUYazuyQ-8239L6zwVS1pNPFsbRBIhL0lSSRjIuaFPQt4_Qq7isc7qheMuoUg-owYygfXAxz8aupvqU101bK8lFoU7-QpWvh8nbGMD5Uv9DQDeBnWNKMzhd5jeZ-aAp0Wvyektel-T1mnwp7tCb24aXboL-XnEXdQHkI1Pr11hjKN348b_W9abcYoT5wSz-KfoNJsnKaA
startdate20140930
enddate20140930
creator
0Liu, Xiujuan
1Pan, Shifeng
2Li, Xiao
3Sun, Qinwei
4Yang, Xiaojing
5Zhao, Ruqian
general
0Springer Berlin Heidelberg
1Springer
2Springer Nature B.V
scope
0CGR
1CUY
2CVF
3ECM
4EIF
5NPM
6AAYXX
7CITATION
8BSHEE
93V.
107QP
117RQ
127RV
137TS
147X7
157XB
1688E
178AO
188C1
198FI
208FJ
218FK
22ABUWG
23AZQEC
24BENPR
25DWQXO
26FYUFA
27GHDGH
28K9-
29K9.
30KB0
31M0R
32M0S
33M1P
34NAPCQ
35PQEST
36PQQKQ
37PQUKI
38PRINS
39Q9U
407X8
sort
creationdate20140930
titleMaternal low-protein diet affects myostatin signaling and protein synthesis in skeletal muscle of offspring piglets at weaning stage
authorLiu, Xiujuan ; Pan, Shifeng ; Li, Xiao ; Sun, Qinwei ; Yang, Xiaojing ; Zhao, Ruqian
facets
frbrtype5
frbrgroupidcdi_FETCH-LOGICAL-c430t-89456f3a7d856cb595d76a4854c39e4b32203bf68737ebef19dcba818683339b0
rsrctypearticles
prefilterarticles
languageeng
creationdate2014
topic
0Amino Acids - blood
1Amino Acids, Branched-Chain - blood
2Animal Nutritional Physiological Phenomena
3Animals
4Branched chain amino acids
5Chemistry
6Chemistry and Materials Science
7Diet, Protein-Restricted - adverse effects
8Eukaryotic Initiation Factor-4E - analysis
9Female
10Genetic translation
11Lactation
12Male
13Maternal Nutritional Physiological Phenomena
14Muscle Proteins - biosynthesis
15Muscle, Skeletal - growth & development
16Muscles
17Myostatin - metabolism
18Nutrition
19Organ Size
20Original Contribution
21Physiological aspects
22Pregnancy
23Prenatal Exposure Delayed Effects
24Protein biosynthesis
25Proteins
26Ribosomal Protein S6 Kinases, 70-kDa - analysis
27RNA
28Signal Transduction
29Sus scrofa
30Swine
31Weaning
toplevelpeer_reviewed
creatorcontrib
0Liu, Xiujuan
1Pan, Shifeng
2Li, Xiao
3Sun, Qinwei
4Yang, Xiaojing
5Zhao, Ruqian
collection
0Medline
1MEDLINE
2MEDLINE (Ovid)
3MEDLINE
4MEDLINE
5PubMed
6CrossRef
7Academic OneFile (A&I only)
8ProQuest Central (Corporate)
9Calcium & Calcified Tissue Abstracts
10Career & Technical Education Database
11Nursing & Allied Health Database
12Physical Education Index
13Health & Medical Collection
14ProQuest Central (purchase pre-March 2016)
15Medical Database (Alumni Edition)
16ProQuest Pharma Collection
17Public Health Database
18Hospital Premium Collection
19Hospital Premium Collection (Alumni Edition)
20ProQuest Central (Alumni) (purchase pre-March 2016)
21ProQuest Central (Alumni Edition)
22ProQuest Central Essentials
23ProQuest Central
24ProQuest Central Korea
25Health Research Premium Collection
26Health Research Premium Collection (Alumni)
27Consumer Health Database (Alumni Edition)
28ProQuest Health & Medical Complete (Alumni)
29Nursing & Allied Health Database (Alumni Edition)
30Consumer Health Database
31Health & Medical Collection (Alumni Edition)
32Medical Database
33Nursing & Allied Health Premium
34ProQuest One Academic Eastern Edition
35ProQuest One Academic
36ProQuest One Academic UKI Edition
37ProQuest Central China
38ProQuest Central Basic
39MEDLINE - Academic
jtitleEuropean journal of nutrition
delivery
delcategoryRemote Search Resource
fulltextno_fulltext
addata
au
0Liu, Xiujuan
1Pan, Shifeng
2Li, Xiao
3Sun, Qinwei
4Yang, Xiaojing
5Zhao, Ruqian
formatjournal
genrearticle
ristypeJOUR
atitleMaternal low-protein diet affects myostatin signaling and protein synthesis in skeletal muscle of offspring piglets at weaning stage
jtitleEuropean journal of nutrition
stitleEur J Nutr
addtitleEur J Nutr
date2014-09-30
risdate2014
volume54
issue6
spage971
epage979
pages971-979
issn1436-6207
eissn1436-6215
abstractPurpose We tested the hypothesis that maternal low-protein (LP) diet during gestation and lactation can program myostatin (MSTN) signaling and protein synthesis in skeletal muscle of offspring at weaning stage (35 days). Methods Fourteen Meishan sows were fed either LP or standard-protein diets throughout gestation and lactation, male offspring piglets were killed at weaning stage and longissimus dorsi (LD) muscles were taken. The cross-sectional areas (CSA) of LD muscles were measured by hematoxylin and eosin staining. The levels of free amino acids in plasma were measured by amino acid auto-analyzer. Proteins and mRNA were determined by Western blot and RT-qPCR, respectively. Results Body weight, LD muscle weight and the myofiber CSA were significantly decreased ( P  < 0.05) in LP piglets; meanwhile, the concentration of branched-chain amino acids was also significantly decreased ( P  < 0.001). MSTN protein content tended to be higher ( P  = 0.098) in LP piglets, while the expression of MSTN receptors, activin type II receptor-beta and transforming growth factor type-beta type I receptor kinase, was significantly up-regulated ( P  < 0.05). Furthermore, p38 mitogen-activated protein kinase, the downstream signaling factor of MSTN, was also enhanced significantly ( P  < 0.05). In addition, key factors of translation initiation, phosphorylated eukaryotic initiation factor 4E and the 70 kDa ribosomal protein S6 kinase, were significantly decreased ( P  < 0.05) in LP piglets. Conclusions Our results suggest that maternal LP diet during gestation and lactation affects MSTN signaling and protein synthesis in skeletal muscle of offspring at weaning stage.
copBerlin/Heidelberg
pubSpringer Berlin Heidelberg
pmid25266448
doi10.1007/s00394-014-0773-1