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Changes in soil bacterial communities and diversity in response to long-term silver exposure

Silver-induced selective pressure is becoming increasingly important due to the growing use of silver (Ag) as an antimicrobial agent in biomedical and commercial products. With demonstrated links between environmental resistomes and clinical pathogens, it is important to identify microbial profiles... Full description

Journal Title: FEMS microbiology ecology 2015-10-01, Vol.91 (10), p.fiv114
Main Author: Vasileiadis, Sotirios
Other Authors: Puglisi, Edoardo , Trevisan, Marco , Scheckel, Kirk G , Langdon, Kate A , McLaughlin, Mike J , Lombi, Enzo , Donner, Erica
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Alma/SFX Local Collection
Publisher: England: Oxford University Press
ID: ISSN: 1574-6941
Link: https://www.ncbi.nlm.nih.gov/pubmed/26391377
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title: Changes in soil bacterial communities and diversity in response to long-term silver exposure
format: Article
creator:
  • Vasileiadis, Sotirios
  • Puglisi, Edoardo
  • Trevisan, Marco
  • Scheckel, Kirk G
  • Langdon, Kate A
  • McLaughlin, Mike J
  • Lombi, Enzo
  • Donner, Erica
subjects:
  • Anti-Bacterial Agents - pharmacology
  • Antimicrobial agents
  • Bacteria
  • Bacteria - classification
  • Bacteria - drug effects
  • Bacteria - genetics
  • bacterial diversity
  • Biodiversity
  • Communities
  • Dosage
  • Drug Resistance, Bacterial - physiology
  • Exposure
  • Immunological tolerance
  • Incubation
  • Lability
  • Microbiology
  • Microorganisms
  • Organic chemistry
  • Real-Time Polymerase Chain Reaction
  • RNA, Ribosomal, 16S - genetics
  • rRNA 16S
  • selective pressure
  • Silver
  • Silver - pharmacology
  • soil
  • Soil - chemistry
  • Soil investigations
  • Soil Microbiology
  • Soil microorganisms
  • Soil Pollutants - pharmacology
  • Soil stresses
  • Soils
  • Speciation
  • Spectroscopy
  • Synchrotron radiation
  • Thin films
  • X ray absorption
ispartof: FEMS microbiology ecology, 2015-10-01, Vol.91 (10), p.fiv114
description: Silver-induced selective pressure is becoming increasingly important due to the growing use of silver (Ag) as an antimicrobial agent in biomedical and commercial products. With demonstrated links between environmental resistomes and clinical pathogens, it is important to identify microbial profiles related to silver tolerance/resistance. We investigated the effects of ionic Ag stress on soil bacterial communities and identified resistant/persistent bacterial populations. Silver treatments of 50–400 mg Ag kg−1 soil were established in five soils. Chemical lability measurements using diffusive gradients in thin-film devices confirmed that significant (albeit decreasing) labile Ag concentrations were present throughout the 9-month incubation period. Synchrotron X-ray absorption near edge structure spectroscopy demonstrated that this decreasing lability was due to changes in the Ag speciation to less soluble forms such as Ag0 and Ag2S. Real-time PCR and Illumina MiSeq screening of 16S rRNA bacterial genes showed β-diversity changes, increasing α-diversity in response to Ag pressure, and immediate and significant reductions in 16S rRNA gene counts with varying degrees of recovery. These effects were more strongly influenced by exposure time than by Ag dose at these rates. Ag-selected dominant OTUs principally resided in known persister taxa (mainly Gram positive), including metal-tolerant bacteria and slow-growing Mycobacteria. High-throughput bacterial DNA sequencing and advanced chemical and spectroscopic techniques revealed complex changes in bacterial abundance and diversity in response to silver-induced selective pressure in soil microbial communities.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 1574-6941
fulltext: fulltext
issn:
  • 1574-6941
  • 0168-6496
  • 1574-6941
url: Link


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creatorVasileiadis, Sotirios ; Puglisi, Edoardo ; Trevisan, Marco ; Scheckel, Kirk G ; Langdon, Kate A ; McLaughlin, Mike J ; Lombi, Enzo ; Donner, Erica
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descriptionSilver-induced selective pressure is becoming increasingly important due to the growing use of silver (Ag) as an antimicrobial agent in biomedical and commercial products. With demonstrated links between environmental resistomes and clinical pathogens, it is important to identify microbial profiles related to silver tolerance/resistance. We investigated the effects of ionic Ag stress on soil bacterial communities and identified resistant/persistent bacterial populations. Silver treatments of 50–400 mg Ag kg−1 soil were established in five soils. Chemical lability measurements using diffusive gradients in thin-film devices confirmed that significant (albeit decreasing) labile Ag concentrations were present throughout the 9-month incubation period. Synchrotron X-ray absorption near edge structure spectroscopy demonstrated that this decreasing lability was due to changes in the Ag speciation to less soluble forms such as Ag0 and Ag2S. Real-time PCR and Illumina MiSeq screening of 16S rRNA bacterial genes showed β-diversity changes, increasing α-diversity in response to Ag pressure, and immediate and significant reductions in 16S rRNA gene counts with varying degrees of recovery. These effects were more strongly influenced by exposure time than by Ag dose at these rates. Ag-selected dominant OTUs principally resided in known persister taxa (mainly Gram positive), including metal-tolerant bacteria and slow-growing Mycobacteria. High-throughput bacterial DNA sequencing and advanced chemical and spectroscopic techniques revealed complex changes in bacterial abundance and diversity in response to silver-induced selective pressure in soil microbial communities.
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subjectAnti-Bacterial Agents - pharmacology ; Antimicrobial agents ; Bacteria ; Bacteria - classification ; Bacteria - drug effects ; Bacteria - genetics ; bacterial diversity ; Biodiversity ; Communities ; Dosage ; Drug Resistance, Bacterial - physiology ; Exposure ; Immunological tolerance ; Incubation ; Lability ; Microbiology ; Microorganisms ; Organic chemistry ; Real-Time Polymerase Chain Reaction ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; selective pressure ; Silver ; Silver - pharmacology ; soil ; Soil - chemistry ; Soil investigations ; Soil Microbiology ; Soil microorganisms ; Soil Pollutants - pharmacology ; Soil stresses ; Soils ; Speciation ; Spectroscopy ; Synchrotron radiation ; Thin films ; X ray absorption
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abstractSilver-induced selective pressure is becoming increasingly important due to the growing use of silver (Ag) as an antimicrobial agent in biomedical and commercial products. With demonstrated links between environmental resistomes and clinical pathogens, it is important to identify microbial profiles related to silver tolerance/resistance. We investigated the effects of ionic Ag stress on soil bacterial communities and identified resistant/persistent bacterial populations. Silver treatments of 50–400 mg Ag kg−1 soil were established in five soils. Chemical lability measurements using diffusive gradients in thin-film devices confirmed that significant (albeit decreasing) labile Ag concentrations were present throughout the 9-month incubation period. Synchrotron X-ray absorption near edge structure spectroscopy demonstrated that this decreasing lability was due to changes in the Ag speciation to less soluble forms such as Ag0 and Ag2S. Real-time PCR and Illumina MiSeq screening of 16S rRNA bacterial genes showed β-diversity changes, increasing α-diversity in response to Ag pressure, and immediate and significant reductions in 16S rRNA gene counts with varying degrees of recovery. These effects were more strongly influenced by exposure time than by Ag dose at these rates. Ag-selected dominant OTUs principally resided in known persister taxa (mainly Gram positive), including metal-tolerant bacteria and slow-growing Mycobacteria. High-throughput bacterial DNA sequencing and advanced chemical and spectroscopic techniques revealed complex changes in bacterial abundance and diversity in response to silver-induced selective pressure in soil microbial communities.
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