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Analysis of microsamples of human faeces: a non-invasive approach to study the bioavailability of fat-soluble bioactive compounds

Introduction Bioavailability is a critical feature in the assessment of the role of micronutrients in human health. Poorly bioavailable micronutrients like carotenoids may reach significant concentrations in the gastrointestinal tract where they may exert biological actions. Purpose We evaluated a s... Full description

Journal Title: European journal of nutrition 2015, Vol.54 (8), p.1371-1378
Main Author: Hernandez-Alvarez, E
Other Authors: Pérez-Sacristán, B. I , Blanco-Navarro, I , Donoso-Navarro, E , Silvestre-Mardomingo, R. A , Granado-Lorencio, F
Format: Electronic Article Electronic Article
Language: English
Subjects:
alpha-Tocopherol - blood
alpha-Tocopherol - pharmacokinetics
Beta carotene
beta Carotene - blood
beta Carotene - pharmacokinetics
Biological Availability
Chemistry
Chemistry and Materials Science
Cross-Over Studies
Double-Blind Method
Esters
Fatty acids
Feces - chemistry
Female
Gastrointestinal system
Humans
Infant
Male
Metabolites
Micronutrients - blood
Micronutrients - pharmacokinetics
Middle Aged
Nutrition
Short Communication
Tocopherols
Vitamin A
Vitamin A - blood
Vitamin A - pharmacokinetics
Vitamin E - blood
Vitamin E - pharmacokinetics
Publisher: Berlin/Heidelberg: Springer Berlin Heidelberg
ID: ISSN: 1436-6207
Link: https://www.ncbi.nlm.nih.gov/pubmed/26026480
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recordid: cdi_proquest_miscellaneous_1735909253
title: Analysis of microsamples of human faeces: a non-invasive approach to study the bioavailability of fat-soluble bioactive compounds
format: Article
creator:
  • Hernandez-Alvarez, E
  • Pérez-Sacristán, B. I
  • Blanco-Navarro, I
  • Donoso-Navarro, E
  • Silvestre-Mardomingo, R. A
  • Granado-Lorencio, F
subjects:
  • alpha-Tocopherol - blood
  • alpha-Tocopherol - pharmacokinetics
  • Beta carotene
  • beta Carotene - blood
  • beta Carotene - pharmacokinetics
  • Biological Availability
  • Chemistry
  • Chemistry and Materials Science
  • Cross-Over Studies
  • Double-Blind Method
  • Esters
  • Fatty acids
  • Feces - chemistry
  • Female
  • Gastrointestinal system
  • Humans
  • Infant
  • Male
  • Metabolites
  • Micronutrients - blood
  • Micronutrients - pharmacokinetics
  • Middle Aged
  • Nutrition
  • Short Communication
  • Tocopherols
  • Vitamin A
  • Vitamin A - blood
  • Vitamin A - pharmacokinetics
  • Vitamin E - blood
  • Vitamin E - pharmacokinetics
ispartof: European journal of nutrition, 2015, Vol.54 (8), p.1371-1378
description: Introduction Bioavailability is a critical feature in the assessment of the role of micronutrients in human health. Poorly bioavailable micronutrients like carotenoids may reach significant concentrations in the gastrointestinal tract where they may exert biological actions. Purpose We evaluated a simple collection protocol to determine vitamin A, E and carotenoids in microsamples of human faeces as a non-invasive approach for nutritional studies. Methods Microsamples of human faeces were collected using a commercially available device, extracted and analysed on two LC systems. Suitability of the protocol was assessed by evaluating several factors including the effect of simulated colonic conditions and two nutritional scenarios with different dietary components, chemical forms, nutritional goals and target groups. Results The protocol was reproducible and representative of a faeces sample. The major dietary and serum carotenoids, and several “unidentified” compounds (possibly metabolites) could be detected, and cis -/ trans -β-carotene profile reflected dietary intervention. In faeces of neonates, free retinol, retinyl and α-tocopheryl acetate (from infant formula), long-chain fatty acid retinyl esters (from human milk), free γ-tocopherol and α-tocopherol could be detected. Conclusion Our results show that the analysis of vitamin A, E and carotenoids in microsamples of human faeces is a suitable, non-invasive approach that may provide relevant information regarding responsiveness, nutrient stability and metabolism and may help assess adequacy of chemical forms and delivery systems reaching the colon.
language: eng
source:
identifier: ISSN: 1436-6207
fulltext: no_fulltext
issn:
  • 1436-6207
  • 1436-6215
url: Link


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titleAnalysis of microsamples of human faeces: a non-invasive approach to study the bioavailability of fat-soluble bioactive compounds
creatorHernandez-Alvarez, E ; Pérez-Sacristán, B. I ; Blanco-Navarro, I ; Donoso-Navarro, E ; Silvestre-Mardomingo, R. A ; Granado-Lorencio, F
creatorcontribHernandez-Alvarez, E ; Pérez-Sacristán, B. I ; Blanco-Navarro, I ; Donoso-Navarro, E ; Silvestre-Mardomingo, R. A ; Granado-Lorencio, F
descriptionIntroduction Bioavailability is a critical feature in the assessment of the role of micronutrients in human health. Poorly bioavailable micronutrients like carotenoids may reach significant concentrations in the gastrointestinal tract where they may exert biological actions. Purpose We evaluated a simple collection protocol to determine vitamin A, E and carotenoids in microsamples of human faeces as a non-invasive approach for nutritional studies. Methods Microsamples of human faeces were collected using a commercially available device, extracted and analysed on two LC systems. Suitability of the protocol was assessed by evaluating several factors including the effect of simulated colonic conditions and two nutritional scenarios with different dietary components, chemical forms, nutritional goals and target groups. Results The protocol was reproducible and representative of a faeces sample. The major dietary and serum carotenoids, and several “unidentified” compounds (possibly metabolites) could be detected, and cis -/ trans -β-carotene profile reflected dietary intervention. In faeces of neonates, free retinol, retinyl and α-tocopheryl acetate (from infant formula), long-chain fatty acid retinyl esters (from human milk), free γ-tocopherol and α-tocopherol could be detected. Conclusion Our results show that the analysis of vitamin A, E and carotenoids in microsamples of human faeces is a suitable, non-invasive approach that may provide relevant information regarding responsiveness, nutrient stability and metabolism and may help assess adequacy of chemical forms and delivery systems reaching the colon.
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subjectalpha-Tocopherol - blood ; alpha-Tocopherol - pharmacokinetics ; Beta carotene ; beta Carotene - blood ; beta Carotene - pharmacokinetics ; Biological Availability ; Chemistry ; Chemistry and Materials Science ; Cross-Over Studies ; Double-Blind Method ; Esters ; Fatty acids ; Feces - chemistry ; Female ; Gastrointestinal system ; Humans ; Infant ; Male ; Metabolites ; Micronutrients - blood ; Micronutrients - pharmacokinetics ; Middle Aged ; Nutrition ; Short Communication ; Tocopherols ; Vitamin A ; Vitamin A - blood ; Vitamin A - pharmacokinetics ; Vitamin E - blood ; Vitamin E - pharmacokinetics
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descriptionIntroduction Bioavailability is a critical feature in the assessment of the role of micronutrients in human health. Poorly bioavailable micronutrients like carotenoids may reach significant concentrations in the gastrointestinal tract where they may exert biological actions. Purpose We evaluated a simple collection protocol to determine vitamin A, E and carotenoids in microsamples of human faeces as a non-invasive approach for nutritional studies. Methods Microsamples of human faeces were collected using a commercially available device, extracted and analysed on two LC systems. Suitability of the protocol was assessed by evaluating several factors including the effect of simulated colonic conditions and two nutritional scenarios with different dietary components, chemical forms, nutritional goals and target groups. Results The protocol was reproducible and representative of a faeces sample. The major dietary and serum carotenoids, and several “unidentified” compounds (possibly metabolites) could be detected, and cis -/ trans -β-carotene profile reflected dietary intervention. In faeces of neonates, free retinol, retinyl and α-tocopheryl acetate (from infant formula), long-chain fatty acid retinyl esters (from human milk), free γ-tocopherol and α-tocopherol could be detected. Conclusion Our results show that the analysis of vitamin A, E and carotenoids in microsamples of human faeces is a suitable, non-invasive approach that may provide relevant information regarding responsiveness, nutrient stability and metabolism and may help assess adequacy of chemical forms and delivery systems reaching the colon.
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titleAnalysis of microsamples of human faeces: a non-invasive approach to study the bioavailability of fat-soluble bioactive compounds
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atitleAnalysis of microsamples of human faeces: a non-invasive approach to study the bioavailability of fat-soluble bioactive compounds
jtitleEuropean journal of nutrition
stitleEur J Nutr
addtitleEur J Nutr
date2015-05-31
risdate2015
volume54
issue8
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pages1371-1378
issn1436-6207
eissn1436-6215
abstractIntroduction Bioavailability is a critical feature in the assessment of the role of micronutrients in human health. Poorly bioavailable micronutrients like carotenoids may reach significant concentrations in the gastrointestinal tract where they may exert biological actions. Purpose We evaluated a simple collection protocol to determine vitamin A, E and carotenoids in microsamples of human faeces as a non-invasive approach for nutritional studies. Methods Microsamples of human faeces were collected using a commercially available device, extracted and analysed on two LC systems. Suitability of the protocol was assessed by evaluating several factors including the effect of simulated colonic conditions and two nutritional scenarios with different dietary components, chemical forms, nutritional goals and target groups. Results The protocol was reproducible and representative of a faeces sample. The major dietary and serum carotenoids, and several “unidentified” compounds (possibly metabolites) could be detected, and cis -/ trans -β-carotene profile reflected dietary intervention. In faeces of neonates, free retinol, retinyl and α-tocopheryl acetate (from infant formula), long-chain fatty acid retinyl esters (from human milk), free γ-tocopherol and α-tocopherol could be detected. Conclusion Our results show that the analysis of vitamin A, E and carotenoids in microsamples of human faeces is a suitable, non-invasive approach that may provide relevant information regarding responsiveness, nutrient stability and metabolism and may help assess adequacy of chemical forms and delivery systems reaching the colon.
copBerlin/Heidelberg
pubSpringer Berlin Heidelberg
pmid26026480
doi10.1007/s00394-015-0939-5