schliessen

Filtern

 

Bibliotheken

Analysis of microsamples of human faeces: a non-invasive approach to study the bioavailability of fat-soluble bioactive compounds

Introduction Bioavailability is a critical feature in the assessment of the role of micronutrients in human health. Poorly bioavailable micronutrients like carotenoids may reach significant concentrations in the gastrointestinal tract where they may exert biological actions. Purpose We evaluated a s... Full description

Journal Title: European journal of nutrition 2015, Vol.54 (8), p.1371-1378
Main Author: Hernandez-Alvarez, E
Other Authors: Pérez-Sacristán, B. I , Blanco-Navarro, I , Donoso-Navarro, E , Silvestre-Mardomingo, R. A , Granado-Lorencio, F
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: Berlin/Heidelberg: Springer Berlin Heidelberg
ID: ISSN: 1436-6207
Link: https://www.ncbi.nlm.nih.gov/pubmed/26026480
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: cdi_proquest_miscellaneous_1735909253
title: Analysis of microsamples of human faeces: a non-invasive approach to study the bioavailability of fat-soluble bioactive compounds
format: Article
creator:
  • Hernandez-Alvarez, E
  • Pérez-Sacristán, B. I
  • Blanco-Navarro, I
  • Donoso-Navarro, E
  • Silvestre-Mardomingo, R. A
  • Granado-Lorencio, F
subjects:
  • alpha-Tocopherol - blood
  • alpha-Tocopherol - pharmacokinetics
  • Beta carotene
  • beta Carotene - blood
  • beta Carotene - pharmacokinetics
  • Biological Availability
  • Chemistry
  • Chemistry and Materials Science
  • Cross-Over Studies
  • Double-Blind Method
  • Esters
  • Fatty acids
  • Feces - chemistry
  • Female
  • Gastrointestinal system
  • Humans
  • Infant
  • Male
  • Metabolites
  • Micronutrients - blood
  • Micronutrients - pharmacokinetics
  • Middle Aged
  • Nutrition
  • Short Communication
  • Tocopherols
  • Vitamin A
  • Vitamin A - blood
  • Vitamin A - pharmacokinetics
  • Vitamin E - blood
  • Vitamin E - pharmacokinetics
ispartof: European journal of nutrition, 2015, Vol.54 (8), p.1371-1378
description: Introduction Bioavailability is a critical feature in the assessment of the role of micronutrients in human health. Poorly bioavailable micronutrients like carotenoids may reach significant concentrations in the gastrointestinal tract where they may exert biological actions. Purpose We evaluated a simple collection protocol to determine vitamin A, E and carotenoids in microsamples of human faeces as a non-invasive approach for nutritional studies. Methods Microsamples of human faeces were collected using a commercially available device, extracted and analysed on two LC systems. Suitability of the protocol was assessed by evaluating several factors including the effect of simulated colonic conditions and two nutritional scenarios with different dietary components, chemical forms, nutritional goals and target groups. Results The protocol was reproducible and representative of a faeces sample. The major dietary and serum carotenoids, and several “unidentified” compounds (possibly metabolites) could be detected, and cis -/ trans -β-carotene profile reflected dietary intervention. In faeces of neonates, free retinol, retinyl and α-tocopheryl acetate (from infant formula), long-chain fatty acid retinyl esters (from human milk), free γ-tocopherol and α-tocopherol could be detected. Conclusion Our results show that the analysis of vitamin A, E and carotenoids in microsamples of human faeces is a suitable, non-invasive approach that may provide relevant information regarding responsiveness, nutrient stability and metabolism and may help assess adequacy of chemical forms and delivery systems reaching the colon.
language: eng
source:
identifier: ISSN: 1436-6207
fulltext: no_fulltext
issn:
  • 1436-6207
  • 1436-6215
url: Link


@attributes
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
RANK2.4020953
LOCALfalse
PrimoNMBib
record
control
sourceidgale_proqu
recordidTN_cdi_proquest_miscellaneous_1735909253
sourceformatXML
sourcesystemPC
galeidA435255900
sourcerecordidA435255900
originalsourceidFETCH-LOGICAL-c382t-c10af6bc368174abd6e5348616b5a278d796def58876064767e1f54ac558ba420
addsrcrecordideNp1kUtv1TAQhSMEoqXwA9ggS2zYpIzt2HHYXVW8pEpsYG1NHLvXlWNf4uRKd8k_xyGlPATywo_5ztF4TlU9p3BJAdrXGYB3TQ1U1NDxrhYPqnPacFlLRsXD-zO0Z9WTnG8BgHFJH1dnTAKTjYLz6tsuYjhln0lyZPRmShnHQ7A_7vtlxEgcWmPzG4Ikplj7eMTsj5bg4TAlNHsyJ5LnZTiReW9J7xMe0QfsffDzaXVxONc5haUPW9nMq9yk8ZCWOOSn1SOHIdtnd_tF9eXd289XH-rrT-8_Xu2ua8MVm2tDAZ3sDZeKtg32g7SCN0pS2QtkrRraTg7WCaVaCbJpZWupEw0aIVSPDYOL6tXmW9r-utg869FnY0PAaNOSNW256KBjghf05V_obVqmMqiNoqr0oH5RNxis9tGleUKzmupdwwUTxQ4KdfkPqqzBlnGnaJ0v738I6CZYs8iTdfow-RGnk6ag19j1Frsuses1di2K5sVdw0s_2uFe8TPnArANyKUUb-z024_-6_odUq-2-Q
sourcetypeAggregation Database
isCDItrue
recordtypearticle
pqid1735186818
display
typearticle
titleAnalysis of microsamples of human faeces: a non-invasive approach to study the bioavailability of fat-soluble bioactive compounds
creatorHernandez-Alvarez, E ; Pérez-Sacristán, B. I ; Blanco-Navarro, I ; Donoso-Navarro, E ; Silvestre-Mardomingo, R. A ; Granado-Lorencio, F
creatorcontribHernandez-Alvarez, E ; Pérez-Sacristán, B. I ; Blanco-Navarro, I ; Donoso-Navarro, E ; Silvestre-Mardomingo, R. A ; Granado-Lorencio, F
descriptionIntroduction Bioavailability is a critical feature in the assessment of the role of micronutrients in human health. Poorly bioavailable micronutrients like carotenoids may reach significant concentrations in the gastrointestinal tract where they may exert biological actions. Purpose We evaluated a simple collection protocol to determine vitamin A, E and carotenoids in microsamples of human faeces as a non-invasive approach for nutritional studies. Methods Microsamples of human faeces were collected using a commercially available device, extracted and analysed on two LC systems. Suitability of the protocol was assessed by evaluating several factors including the effect of simulated colonic conditions and two nutritional scenarios with different dietary components, chemical forms, nutritional goals and target groups. Results The protocol was reproducible and representative of a faeces sample. The major dietary and serum carotenoids, and several “unidentified” compounds (possibly metabolites) could be detected, and cis -/ trans -β-carotene profile reflected dietary intervention. In faeces of neonates, free retinol, retinyl and α-tocopheryl acetate (from infant formula), long-chain fatty acid retinyl esters (from human milk), free γ-tocopherol and α-tocopherol could be detected. Conclusion Our results show that the analysis of vitamin A, E and carotenoids in microsamples of human faeces is a suitable, non-invasive approach that may provide relevant information regarding responsiveness, nutrient stability and metabolism and may help assess adequacy of chemical forms and delivery systems reaching the colon.
identifier
0ISSN: 1436-6207
1EISSN: 1436-6215
2DOI: 10.1007/s00394-015-0939-5
3PMID: 26026480
languageeng
publisherBerlin/Heidelberg: Springer Berlin Heidelberg
subjectalpha-Tocopherol - blood ; alpha-Tocopherol - pharmacokinetics ; Beta carotene ; beta Carotene - blood ; beta Carotene - pharmacokinetics ; Biological Availability ; Chemistry ; Chemistry and Materials Science ; Cross-Over Studies ; Double-Blind Method ; Esters ; Fatty acids ; Feces - chemistry ; Female ; Gastrointestinal system ; Humans ; Infant ; Male ; Metabolites ; Micronutrients - blood ; Micronutrients - pharmacokinetics ; Middle Aged ; Nutrition ; Short Communication ; Tocopherols ; Vitamin A ; Vitamin A - blood ; Vitamin A - pharmacokinetics ; Vitamin E - blood ; Vitamin E - pharmacokinetics
ispartofEuropean journal of nutrition, 2015, Vol.54 (8), p.1371-1378
rights
0Springer-Verlag Berlin Heidelberg 2015
1COPYRIGHT 2015 Springer
lds50peer_reviewed
citesFETCH-LOGICAL-c382t-c10af6bc368174abd6e5348616b5a278d796def58876064767e1f54ac558ba420
links
openurl$$Topenurl_article
thumbnail$$Usyndetics_thumb_exl
backlink$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26026480$$D View this record in MEDLINE/PubMed
search
creatorcontrib
0Hernandez-Alvarez, E
1Pérez-Sacristán, B. I
2Blanco-Navarro, I
3Donoso-Navarro, E
4Silvestre-Mardomingo, R. A
5Granado-Lorencio, F
title
0Analysis of microsamples of human faeces: a non-invasive approach to study the bioavailability of fat-soluble bioactive compounds
1European journal of nutrition
addtitle
0Eur J Nutr
1Eur J Nutr
descriptionIntroduction Bioavailability is a critical feature in the assessment of the role of micronutrients in human health. Poorly bioavailable micronutrients like carotenoids may reach significant concentrations in the gastrointestinal tract where they may exert biological actions. Purpose We evaluated a simple collection protocol to determine vitamin A, E and carotenoids in microsamples of human faeces as a non-invasive approach for nutritional studies. Methods Microsamples of human faeces were collected using a commercially available device, extracted and analysed on two LC systems. Suitability of the protocol was assessed by evaluating several factors including the effect of simulated colonic conditions and two nutritional scenarios with different dietary components, chemical forms, nutritional goals and target groups. Results The protocol was reproducible and representative of a faeces sample. The major dietary and serum carotenoids, and several “unidentified” compounds (possibly metabolites) could be detected, and cis -/ trans -β-carotene profile reflected dietary intervention. In faeces of neonates, free retinol, retinyl and α-tocopheryl acetate (from infant formula), long-chain fatty acid retinyl esters (from human milk), free γ-tocopherol and α-tocopherol could be detected. Conclusion Our results show that the analysis of vitamin A, E and carotenoids in microsamples of human faeces is a suitable, non-invasive approach that may provide relevant information regarding responsiveness, nutrient stability and metabolism and may help assess adequacy of chemical forms and delivery systems reaching the colon.
subject
0alpha-Tocopherol - blood
1alpha-Tocopherol - pharmacokinetics
2Beta carotene
3beta Carotene - blood
4beta Carotene - pharmacokinetics
5Biological Availability
6Chemistry
7Chemistry and Materials Science
8Cross-Over Studies
9Double-Blind Method
10Esters
11Fatty acids
12Feces - chemistry
13Female
14Gastrointestinal system
15Humans
16Infant
17Male
18Metabolites
19Micronutrients - blood
20Micronutrients - pharmacokinetics
21Middle Aged
22Nutrition
23Short Communication
24Tocopherols
25Vitamin A
26Vitamin A - blood
27Vitamin A - pharmacokinetics
28Vitamin E - blood
29Vitamin E - pharmacokinetics
issn
01436-6207
11436-6215
fulltextfalse
rsrctypearticle
creationdate2015
recordtypearticle
recordideNp1kUtv1TAQhSMEoqXwA9ggS2zYpIzt2HHYXVW8pEpsYG1NHLvXlWNf4uRKd8k_xyGlPATywo_5ztF4TlU9p3BJAdrXGYB3TQ1U1NDxrhYPqnPacFlLRsXD-zO0Z9WTnG8BgHFJH1dnTAKTjYLz6tsuYjhln0lyZPRmShnHQ7A_7vtlxEgcWmPzG4Ikplj7eMTsj5bg4TAlNHsyJ5LnZTiReW9J7xMe0QfsffDzaXVxONc5haUPW9nMq9yk8ZCWOOSn1SOHIdtnd_tF9eXd289XH-rrT-8_Xu2ua8MVm2tDAZ3sDZeKtg32g7SCN0pS2QtkrRraTg7WCaVaCbJpZWupEw0aIVSPDYOL6tXmW9r-utg869FnY0PAaNOSNW256KBjghf05V_obVqmMqiNoqr0oH5RNxis9tGleUKzmupdwwUTxQ4KdfkPqqzBlnGnaJ0v738I6CZYs8iTdfow-RGnk6ag19j1Frsuses1di2K5sVdw0s_2uFe8TPnArANyKUUb-z024_-6_odUq-2-Q
startdate20150531
enddate20150531
creator
0Hernandez-Alvarez, E
1Pérez-Sacristán, B. I
2Blanco-Navarro, I
3Donoso-Navarro, E
4Silvestre-Mardomingo, R. A
5Granado-Lorencio, F
general
0Springer Berlin Heidelberg
1Springer
2Springer Nature B.V
scope
0CGR
1CUY
2CVF
3ECM
4EIF
5NPM
6AAYXX
7CITATION
8BSHEE
93V.
107QP
117RQ
127RV
137TS
147X7
157XB
1688E
178AO
188C1
198FI
208FJ
218FK
22ABUWG
23AZQEC
24BENPR
25DWQXO
26FYUFA
27GHDGH
28K9-
29K9.
30KB0
31M0R
32M0S
33M1P
34NAPCQ
35PQEST
36PQQKQ
37PQUKI
38PRINS
39Q9U
407X8
sort
creationdate20150531
titleAnalysis of microsamples of human faeces: a non-invasive approach to study the bioavailability of fat-soluble bioactive compounds
authorHernandez-Alvarez, E ; Pérez-Sacristán, B. I ; Blanco-Navarro, I ; Donoso-Navarro, E ; Silvestre-Mardomingo, R. A ; Granado-Lorencio, F
facets
frbrtype5
frbrgroupidcdi_FETCH-LOGICAL-c382t-c10af6bc368174abd6e5348616b5a278d796def58876064767e1f54ac558ba420
rsrctypearticles
prefilterarticles
languageeng
creationdate2015
topic
0alpha-Tocopherol - blood
1alpha-Tocopherol - pharmacokinetics
2Beta carotene
3beta Carotene - blood
4beta Carotene - pharmacokinetics
5Biological Availability
6Chemistry
7Chemistry and Materials Science
8Cross-Over Studies
9Double-Blind Method
10Esters
11Fatty acids
12Feces - chemistry
13Female
14Gastrointestinal system
15Humans
16Infant
17Male
18Metabolites
19Micronutrients - blood
20Micronutrients - pharmacokinetics
21Middle Aged
22Nutrition
23Short Communication
24Tocopherols
25Vitamin A
26Vitamin A - blood
27Vitamin A - pharmacokinetics
28Vitamin E - blood
29Vitamin E - pharmacokinetics
toplevelpeer_reviewed
creatorcontrib
0Hernandez-Alvarez, E
1Pérez-Sacristán, B. I
2Blanco-Navarro, I
3Donoso-Navarro, E
4Silvestre-Mardomingo, R. A
5Granado-Lorencio, F
collection
0Medline
1MEDLINE
2MEDLINE (Ovid)
3MEDLINE
4MEDLINE
5PubMed
6CrossRef
7Academic OneFile (A&I only)
8ProQuest Central (Corporate)
9Calcium & Calcified Tissue Abstracts
10Career & Technical Education Database
11Nursing & Allied Health Database
12Physical Education Index
13Health & Medical Collection
14ProQuest Central (purchase pre-March 2016)
15Medical Database (Alumni Edition)
16ProQuest Pharma Collection
17Public Health Database
18Hospital Premium Collection
19Hospital Premium Collection (Alumni Edition)
20ProQuest Central (Alumni) (purchase pre-March 2016)
21ProQuest Central (Alumni Edition)
22ProQuest Central Essentials
23ProQuest Central
24ProQuest Central Korea
25Health Research Premium Collection
26Health Research Premium Collection (Alumni)
27Consumer Health Database (Alumni Edition)
28ProQuest Health & Medical Complete (Alumni)
29Nursing & Allied Health Database (Alumni Edition)
30Consumer Health Database
31Health & Medical Collection (Alumni Edition)
32Medical Database
33Nursing & Allied Health Premium
34ProQuest One Academic Eastern Edition
35ProQuest One Academic
36ProQuest One Academic UKI Edition
37ProQuest Central China
38ProQuest Central Basic
39MEDLINE - Academic
jtitleEuropean journal of nutrition
delivery
delcategoryRemote Search Resource
fulltextno_fulltext
addata
au
0Hernandez-Alvarez, E
1Pérez-Sacristán, B. I
2Blanco-Navarro, I
3Donoso-Navarro, E
4Silvestre-Mardomingo, R. A
5Granado-Lorencio, F
formatjournal
genrearticle
ristypeJOUR
atitleAnalysis of microsamples of human faeces: a non-invasive approach to study the bioavailability of fat-soluble bioactive compounds
jtitleEuropean journal of nutrition
stitleEur J Nutr
addtitleEur J Nutr
date2015-05-31
risdate2015
volume54
issue8
spage1371
epage1378
pages1371-1378
issn1436-6207
eissn1436-6215
abstractIntroduction Bioavailability is a critical feature in the assessment of the role of micronutrients in human health. Poorly bioavailable micronutrients like carotenoids may reach significant concentrations in the gastrointestinal tract where they may exert biological actions. Purpose We evaluated a simple collection protocol to determine vitamin A, E and carotenoids in microsamples of human faeces as a non-invasive approach for nutritional studies. Methods Microsamples of human faeces were collected using a commercially available device, extracted and analysed on two LC systems. Suitability of the protocol was assessed by evaluating several factors including the effect of simulated colonic conditions and two nutritional scenarios with different dietary components, chemical forms, nutritional goals and target groups. Results The protocol was reproducible and representative of a faeces sample. The major dietary and serum carotenoids, and several “unidentified” compounds (possibly metabolites) could be detected, and cis -/ trans -β-carotene profile reflected dietary intervention. In faeces of neonates, free retinol, retinyl and α-tocopheryl acetate (from infant formula), long-chain fatty acid retinyl esters (from human milk), free γ-tocopherol and α-tocopherol could be detected. Conclusion Our results show that the analysis of vitamin A, E and carotenoids in microsamples of human faeces is a suitable, non-invasive approach that may provide relevant information regarding responsiveness, nutrient stability and metabolism and may help assess adequacy of chemical forms and delivery systems reaching the colon.
copBerlin/Heidelberg
pubSpringer Berlin Heidelberg
pmid26026480
doi10.1007/s00394-015-0939-5