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Isolation and phenotypic characterization of Pseudomonas aeruginosa pseudorevertants containing suppressors of the catabolite repression control-defective crc-10 allele

The amiE gene encodes an aliphatic amidase capable of converting fluoroacetamide to the toxic compound fluoroacetate and is one of many genes whose expression is subject to catabolite repression control in Pseudomonas aeruginosa. The protein product of the crc gene, Crc, is required for repression o... Full description

Journal Title: FEMS microbiology letters 2001, Vol.196 (2), p.87-92
Main Author: Collier, David N
Other Authors: Spence, Cheryl , Cox, Mary J , Phibbs, Paul V
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Alma/SFX Local Collection
Publisher: Oxford: Elsevier B.V
ID: ISSN: 0378-1097
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recordid: cdi_proquest_miscellaneous_17824191
title: Isolation and phenotypic characterization of Pseudomonas aeruginosa pseudorevertants containing suppressors of the catabolite repression control-defective crc-10 allele
format: Article
creator:
  • Collier, David N
  • Spence, Cheryl
  • Cox, Mary J
  • Phibbs, Paul V
subjects:
  • amidase
  • Amidohydrolases - metabolism
  • amiE gene
  • Bacterial Proteins - genetics
  • Bacteriology
  • Biological and medical sciences
  • Biological Transport
  • Blotting, Western
  • Catabolite repression control
  • crc gene
  • Fluoroacetamide
  • Fluoroacetates - pharmacology
  • Fundamental and applied biological sciences. Psychology
  • Genetics
  • glucose-6-phosphate dehydrogenase
  • mannitol dehydrogenase
  • Metabolism. Enzymes
  • Microbiology
  • Mutation
  • Operon
  • Phenotype
  • Pseudomonas aeruginosa
  • Pseudomonas aeruginosa - genetics
  • Pseudomonas aeruginosa - growth & development
  • Pseudomonas aeruginosa - isolation & purification
  • pyocyanin
  • Repressor Proteins - genetics
  • Suppressor mutation
ispartof: FEMS microbiology letters, 2001, Vol.196 (2), p.87-92
description: The amiE gene encodes an aliphatic amidase capable of converting fluoroacetamide to the toxic compound fluoroacetate and is one of many genes whose expression is subject to catabolite repression control in Pseudomonas aeruginosa. The protein product of the crc gene, Crc, is required for repression of amiE and most other genes subject to catabolite repression control in this bacterium. When grown in a carbon source such as succinate, wild-type P. aeruginosa is insensitive to fluoroacetamide (due to repression of amiE expression). In contrast, mutants harboring the crc-10 null allele cannot grow in the presence of fluoroacetamide (due to lack of repression of amiE). Selection for succinate-dependent, fluoroacetamide-resistant derivatives of the crc-10 mutant yielded three independent pseudorevertants containing suppressors that restored a degree of catabolite repression control. Synthesis of Crc protein was not reestablished in these pseudorevertants. All three suppressors of crc-10 were extragenic, and all three also suppressed a Δ crc::tetA allele. In each of the three pseudorevertants, catabolite repression control of amidase expression was restored. Catabolite repression control of mannitol dehydrogenase production was also restored in two of the three isolates. None of the suppressors restored repression of glucose-6-phosphate dehydrogenase or pyocyanin production.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0378-1097
fulltext: fulltext
issn:
  • 0378-1097
  • 1574-6968
url: Link


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titleIsolation and phenotypic characterization of Pseudomonas aeruginosa pseudorevertants containing suppressors of the catabolite repression control-defective crc-10 allele
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creatorCollier, David N ; Spence, Cheryl ; Cox, Mary J ; Phibbs, Paul V
creatorcontribCollier, David N ; Spence, Cheryl ; Cox, Mary J ; Phibbs, Paul V
descriptionThe amiE gene encodes an aliphatic amidase capable of converting fluoroacetamide to the toxic compound fluoroacetate and is one of many genes whose expression is subject to catabolite repression control in Pseudomonas aeruginosa. The protein product of the crc gene, Crc, is required for repression of amiE and most other genes subject to catabolite repression control in this bacterium. When grown in a carbon source such as succinate, wild-type P. aeruginosa is insensitive to fluoroacetamide (due to repression of amiE expression). In contrast, mutants harboring the crc-10 null allele cannot grow in the presence of fluoroacetamide (due to lack of repression of amiE). Selection for succinate-dependent, fluoroacetamide-resistant derivatives of the crc-10 mutant yielded three independent pseudorevertants containing suppressors that restored a degree of catabolite repression control. Synthesis of Crc protein was not reestablished in these pseudorevertants. All three suppressors of crc-10 were extragenic, and all three also suppressed a Δ crc::tetA allele. In each of the three pseudorevertants, catabolite repression control of amidase expression was restored. Catabolite repression control of mannitol dehydrogenase production was also restored in two of the three isolates. None of the suppressors restored repression of glucose-6-phosphate dehydrogenase or pyocyanin production.
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1EISSN: 1574-6968
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languageeng
publisherOxford: Elsevier B.V
subjectamidase ; Amidohydrolases - metabolism ; amiE gene ; Bacterial Proteins - genetics ; Bacteriology ; Biological and medical sciences ; Biological Transport ; Blotting, Western ; Catabolite repression control ; crc gene ; Fluoroacetamide ; Fluoroacetates - pharmacology ; Fundamental and applied biological sciences. Psychology ; Genetics ; glucose-6-phosphate dehydrogenase ; mannitol dehydrogenase ; Metabolism. Enzymes ; Microbiology ; Mutation ; Operon ; Phenotype ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - genetics ; Pseudomonas aeruginosa - growth & development ; Pseudomonas aeruginosa - isolation & purification ; pyocyanin ; Repressor Proteins - genetics ; Suppressor mutation
ispartofFEMS microbiology letters, 2001, Vol.196 (2), p.87-92
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addtitleFEMS Microbiol Lett
descriptionThe amiE gene encodes an aliphatic amidase capable of converting fluoroacetamide to the toxic compound fluoroacetate and is one of many genes whose expression is subject to catabolite repression control in Pseudomonas aeruginosa. The protein product of the crc gene, Crc, is required for repression of amiE and most other genes subject to catabolite repression control in this bacterium. When grown in a carbon source such as succinate, wild-type P. aeruginosa is insensitive to fluoroacetamide (due to repression of amiE expression). In contrast, mutants harboring the crc-10 null allele cannot grow in the presence of fluoroacetamide (due to lack of repression of amiE). Selection for succinate-dependent, fluoroacetamide-resistant derivatives of the crc-10 mutant yielded three independent pseudorevertants containing suppressors that restored a degree of catabolite repression control. Synthesis of Crc protein was not reestablished in these pseudorevertants. All three suppressors of crc-10 were extragenic, and all three also suppressed a Δ crc::tetA allele. In each of the three pseudorevertants, catabolite repression control of amidase expression was restored. Catabolite repression control of mannitol dehydrogenase production was also restored in two of the three isolates. None of the suppressors restored repression of glucose-6-phosphate dehydrogenase or pyocyanin production.
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abstractThe amiE gene encodes an aliphatic amidase capable of converting fluoroacetamide to the toxic compound fluoroacetate and is one of many genes whose expression is subject to catabolite repression control in Pseudomonas aeruginosa. The protein product of the crc gene, Crc, is required for repression of amiE and most other genes subject to catabolite repression control in this bacterium. When grown in a carbon source such as succinate, wild-type P. aeruginosa is insensitive to fluoroacetamide (due to repression of amiE expression). In contrast, mutants harboring the crc-10 null allele cannot grow in the presence of fluoroacetamide (due to lack of repression of amiE). Selection for succinate-dependent, fluoroacetamide-resistant derivatives of the crc-10 mutant yielded three independent pseudorevertants containing suppressors that restored a degree of catabolite repression control. Synthesis of Crc protein was not reestablished in these pseudorevertants. All three suppressors of crc-10 were extragenic, and all three also suppressed a Δ crc::tetA allele. In each of the three pseudorevertants, catabolite repression control of amidase expression was restored. Catabolite repression control of mannitol dehydrogenase production was also restored in two of the three isolates. None of the suppressors restored repression of glucose-6-phosphate dehydrogenase or pyocyanin production.
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pubElsevier B.V
pmid11267761
doi10.1016/S0378-1097(01)00046-5