schliessen

Filtern

 

Bibliotheken

Increased Expression and Cellular Localization of P2X7R in Cortical Lesions of Patients With Focal Cortical Dysplasia

Focal cortical dysplasias (FCDs) are major brain malformations that commonly lead to medically intractable epilepsy. The purinergic ionotropic P2X7 receptor (P2X7R) is an atypical P2X subtype that gates calcium and sodium ions. Previous animal studies have suggested that P2X7R is a contributing fact... Full description

Journal Title: Journal of neuropathology and experimental neurology 2016-01, Vol.75 (1), p.61-68
Main Author: Wei, Yu-Jia
Other Authors: Guo, Wei , Sun, Fei-Ji , Fu, Wan-Lei , Zheng, Da-Hai , Chen, Xin , Li, Song , Zang, Zhen-Le , Zhang, Chun-Qing , Liu, Shi-Yong , Yang, Hui
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: England: Oxford University Press
ID: ISSN: 0022-3069
Link: https://www.ncbi.nlm.nih.gov/pubmed/26671983
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: cdi_proquest_miscellaneous_1793912308
title: Increased Expression and Cellular Localization of P2X7R in Cortical Lesions of Patients With Focal Cortical Dysplasia
format: Article
creator:
  • Wei, Yu-Jia
  • Guo, Wei
  • Sun, Fei-Ji
  • Fu, Wan-Lei
  • Zheng, Da-Hai
  • Chen, Xin
  • Li, Song
  • Zang, Zhen-Le
  • Zhang, Chun-Qing
  • Liu, Shi-Yong
  • Yang, Hui
subjects:
  • Adolescent
  • Cerebral Cortex - chemistry
  • Cerebral Cortex - metabolism
  • Cerebral Cortex - pathology
  • Child
  • Child, Preschool
  • Female
  • Gene Expression Regulation
  • Humans
  • Infant
  • Male
  • Malformations of Cortical Development - diagnosis
  • Malformations of Cortical Development - genetics
  • Malformations of Cortical Development - metabolism
  • Receptors, Purinergic P2X7 - analysis
  • Receptors, Purinergic P2X7 - biosynthesis
  • Receptors, Purinergic P2X7 - genetics
  • Young Adult
ispartof: Journal of neuropathology and experimental neurology, 2016-01, Vol.75 (1), p.61-68
description: Focal cortical dysplasias (FCDs) are major brain malformations that commonly lead to medically intractable epilepsy. The purinergic ionotropic P2X7 receptor (P2X7R) is an atypical P2X subtype that gates calcium and sodium ions. Previous animal studies have suggested that P2X7R is a contributing factor in epileptogenesis. This study aimed to define the distribution and expression of P2X7R in 35 FCD patient-surgical-resection specimens relative to autopsy control samples (n = 8). Immunohistochemical colocalization assays revealed that P2X7R was primarily expressed in neurons, astrocytes, and microglia. In FCD samples, P2X7R protein levels were increased in abnormal cell types such as dysmorphic neurons and balloon cells, which are characteristic of FCD. By real-time PCR and Western blotting, P2X7R mRNA and protein expression levels were elevated in FCD patient samples vs control samples; P2X7R expression was also higher in FCDII vs FCDIa patient samples. Because interleukin-1β is a downstream factor of the P2X7R signaling pathway, we determined that there was also moderate-to-strong interleukin-1β expression in the dysmorphic neurons, balloon cells, and microglia in FCD patient lesions. These results indicate that increasing P2X7R levels may contribute to the pathogenesis of human FCD and that P2X7R represents a potential anti-epileptogenic target.
language: eng
source:
identifier: ISSN: 0022-3069
fulltext: no_fulltext
issn:
  • 0022-3069
  • 1554-6578
url: Link


@attributes
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
RANK2.454358
LOCALfalse
PrimoNMBib
record
control
sourceidproquest_cross
recordidTN_cdi_proquest_miscellaneous_1793912308
sourceformatXML
sourcesystemPC
oup_id10.1093/jnen/nlv003
sourcerecordid1793912308
originalsourceidFETCH-LOGICAL-13718-db6b8ebcaa5ca1af633832b9573391c6f3776b545de19fa4d6e9061efd51b23f0
addsrcrecordideNp9kUFv1DAQhS0EokvhxB35hJBQ2nEc2_ERbVuotBIIgeBmOclEm-K1g51Q2l-PQ1q49WTZ73tPM8-EvGRwwkDz0yuP_tS7XwD8EdkwIapCClU_JhuAsiw4SH1EnqV0BQAadPWUHJVSKqZrviHzpW8j2oQdPf89RkxpCJ5a39EtOjc7G-kutNYNt3ZalNDTT-V39ZkOnm5DnIas0R0urvRXzBj6KdFvw7SnF4v1P3d2k0Zn02Cfkye9dQlf3J3H5OvF-Zfth2L38f3l9t2uYFyxuuga2dTYtNaK1jLbS85rXjZaKM41a2XPlZKNqESHTPe26iRqkAz7TrCm5D0ckzdr7hjDzxnTZA5DavNi1mOYk2FK56CSQ53RtyvaxpBSxN6McTjYeGMYmKVns_Rs1p4z_eoueG4O2P1j74vNQLUC18FNGNMPN19jNHu0btqb_BMgQJVFCUwCy7dieVqmeL3awjw-OMAf8suXWA
sourcetypeAggregation Database
isCDItrue
recordtypearticle
pqid1793912308
display
typearticle
titleIncreased Expression and Cellular Localization of P2X7R in Cortical Lesions of Patients With Focal Cortical Dysplasia
creatorWei, Yu-Jia ; Guo, Wei ; Sun, Fei-Ji ; Fu, Wan-Lei ; Zheng, Da-Hai ; Chen, Xin ; Li, Song ; Zang, Zhen-Le ; Zhang, Chun-Qing ; Liu, Shi-Yong ; Yang, Hui
creatorcontribWei, Yu-Jia ; Guo, Wei ; Sun, Fei-Ji ; Fu, Wan-Lei ; Zheng, Da-Hai ; Chen, Xin ; Li, Song ; Zang, Zhen-Le ; Zhang, Chun-Qing ; Liu, Shi-Yong ; Yang, Hui
descriptionFocal cortical dysplasias (FCDs) are major brain malformations that commonly lead to medically intractable epilepsy. The purinergic ionotropic P2X7 receptor (P2X7R) is an atypical P2X subtype that gates calcium and sodium ions. Previous animal studies have suggested that P2X7R is a contributing factor in epileptogenesis. This study aimed to define the distribution and expression of P2X7R in 35 FCD patient-surgical-resection specimens relative to autopsy control samples (n = 8). Immunohistochemical colocalization assays revealed that P2X7R was primarily expressed in neurons, astrocytes, and microglia. In FCD samples, P2X7R protein levels were increased in abnormal cell types such as dysmorphic neurons and balloon cells, which are characteristic of FCD. By real-time PCR and Western blotting, P2X7R mRNA and protein expression levels were elevated in FCD patient samples vs control samples; P2X7R expression was also higher in FCDII vs FCDIa patient samples. Because interleukin-1β is a downstream factor of the P2X7R signaling pathway, we determined that there was also moderate-to-strong interleukin-1β expression in the dysmorphic neurons, balloon cells, and microglia in FCD patient lesions. These results indicate that increasing P2X7R levels may contribute to the pathogenesis of human FCD and that P2X7R represents a potential anti-epileptogenic target.
identifier
0ISSN: 0022-3069
1EISSN: 1554-6578
2DOI: 10.1093/jnen/nlv003
3PMID: 26671983
languageeng
publisherEngland: Oxford University Press
subjectAdolescent ; Cerebral Cortex - chemistry ; Cerebral Cortex - metabolism ; Cerebral Cortex - pathology ; Child ; Child, Preschool ; Female ; Gene Expression Regulation ; Humans ; Infant ; Male ; Malformations of Cortical Development - diagnosis ; Malformations of Cortical Development - genetics ; Malformations of Cortical Development - metabolism ; Receptors, Purinergic P2X7 - analysis ; Receptors, Purinergic P2X7 - biosynthesis ; Receptors, Purinergic P2X7 - genetics ; Young Adult
ispartofJournal of neuropathology and experimental neurology, 2016-01, Vol.75 (1), p.61-68
rights
02015 American Association of Neuropathologists, Inc. All rights reserved. 2015
12016 by American Association of Neuropathologists, Inc.
lds50peer_reviewed
oafree_for_read
citesFETCH-LOGICAL-13718-db6b8ebcaa5ca1af633832b9573391c6f3776b545de19fa4d6e9061efd51b23f0
links
openurl$$Topenurl_article
thumbnail$$Usyndetics_thumb_exl
backlink$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26671983$$D View this record in MEDLINE/PubMed
search
creatorcontrib
0Wei, Yu-Jia
1Guo, Wei
2Sun, Fei-Ji
3Fu, Wan-Lei
4Zheng, Da-Hai
5Chen, Xin
6Li, Song
7Zang, Zhen-Le
8Zhang, Chun-Qing
9Liu, Shi-Yong
10Yang, Hui
title
0Increased Expression and Cellular Localization of P2X7R in Cortical Lesions of Patients With Focal Cortical Dysplasia
1Journal of neuropathology and experimental neurology
addtitleJ Neuropathol Exp Neurol
descriptionFocal cortical dysplasias (FCDs) are major brain malformations that commonly lead to medically intractable epilepsy. The purinergic ionotropic P2X7 receptor (P2X7R) is an atypical P2X subtype that gates calcium and sodium ions. Previous animal studies have suggested that P2X7R is a contributing factor in epileptogenesis. This study aimed to define the distribution and expression of P2X7R in 35 FCD patient-surgical-resection specimens relative to autopsy control samples (n = 8). Immunohistochemical colocalization assays revealed that P2X7R was primarily expressed in neurons, astrocytes, and microglia. In FCD samples, P2X7R protein levels were increased in abnormal cell types such as dysmorphic neurons and balloon cells, which are characteristic of FCD. By real-time PCR and Western blotting, P2X7R mRNA and protein expression levels were elevated in FCD patient samples vs control samples; P2X7R expression was also higher in FCDII vs FCDIa patient samples. Because interleukin-1β is a downstream factor of the P2X7R signaling pathway, we determined that there was also moderate-to-strong interleukin-1β expression in the dysmorphic neurons, balloon cells, and microglia in FCD patient lesions. These results indicate that increasing P2X7R levels may contribute to the pathogenesis of human FCD and that P2X7R represents a potential anti-epileptogenic target.
subject
0Adolescent
1Cerebral Cortex - chemistry
2Cerebral Cortex - metabolism
3Cerebral Cortex - pathology
4Child
5Child, Preschool
6Female
7Gene Expression Regulation
8Humans
9Infant
10Male
11Malformations of Cortical Development - diagnosis
12Malformations of Cortical Development - genetics
13Malformations of Cortical Development - metabolism
14Receptors, Purinergic P2X7 - analysis
15Receptors, Purinergic P2X7 - biosynthesis
16Receptors, Purinergic P2X7 - genetics
17Young Adult
issn
00022-3069
11554-6578
fulltextfalse
rsrctypearticle
creationdate2016
recordtypearticle
recordideNp9kUFv1DAQhS0EokvhxB35hJBQ2nEc2_ERbVuotBIIgeBmOclEm-K1g51Q2l-PQ1q49WTZ73tPM8-EvGRwwkDz0yuP_tS7XwD8EdkwIapCClU_JhuAsiw4SH1EnqV0BQAadPWUHJVSKqZrviHzpW8j2oQdPf89RkxpCJ5a39EtOjc7G-kutNYNt3ZalNDTT-V39ZkOnm5DnIas0R0urvRXzBj6KdFvw7SnF4v1P3d2k0Zn02Cfkye9dQlf3J3H5OvF-Zfth2L38f3l9t2uYFyxuuga2dTYtNaK1jLbS85rXjZaKM41a2XPlZKNqESHTPe26iRqkAz7TrCm5D0ckzdr7hjDzxnTZA5DavNi1mOYk2FK56CSQ53RtyvaxpBSxN6McTjYeGMYmKVns_Rs1p4z_eoueG4O2P1j74vNQLUC18FNGNMPN19jNHu0btqb_BMgQJVFCUwCy7dieVqmeL3awjw-OMAf8suXWA
startdate201601
enddate201601
creator
0Wei, Yu-Jia
1Guo, Wei
2Sun, Fei-Ji
3Fu, Wan-Lei
4Zheng, Da-Hai
5Chen, Xin
6Li, Song
7Zang, Zhen-Le
8Zhang, Chun-Qing
9Liu, Shi-Yong
10Yang, Hui
general
0Oxford University Press
1by American Association of Neuropathologists, Inc
scope
0CGR
1CUY
2CVF
3ECM
4EIF
5NPM
6AAYXX
7CITATION
87X8
sort
creationdate201601
titleIncreased Expression and Cellular Localization of P2X7R in Cortical Lesions of Patients With Focal Cortical Dysplasia
authorWei, Yu-Jia ; Guo, Wei ; Sun, Fei-Ji ; Fu, Wan-Lei ; Zheng, Da-Hai ; Chen, Xin ; Li, Song ; Zang, Zhen-Le ; Zhang, Chun-Qing ; Liu, Shi-Yong ; Yang, Hui
facets
frbrtype5
frbrgroupidcdi_FETCH-LOGICAL-13718-db6b8ebcaa5ca1af633832b9573391c6f3776b545de19fa4d6e9061efd51b23f0
rsrctypearticles
prefilterarticles
languageeng
creationdate2016
topic
0Adolescent
1Cerebral Cortex - chemistry
2Cerebral Cortex - metabolism
3Cerebral Cortex - pathology
4Child
5Child, Preschool
6Female
7Gene Expression Regulation
8Humans
9Infant
10Male
11Malformations of Cortical Development - diagnosis
12Malformations of Cortical Development - genetics
13Malformations of Cortical Development - metabolism
14Receptors, Purinergic P2X7 - analysis
15Receptors, Purinergic P2X7 - biosynthesis
16Receptors, Purinergic P2X7 - genetics
17Young Adult
toplevelpeer_reviewed
creatorcontrib
0Wei, Yu-Jia
1Guo, Wei
2Sun, Fei-Ji
3Fu, Wan-Lei
4Zheng, Da-Hai
5Chen, Xin
6Li, Song
7Zang, Zhen-Le
8Zhang, Chun-Qing
9Liu, Shi-Yong
10Yang, Hui
collection
0Medline
1MEDLINE
2MEDLINE (Ovid)
3MEDLINE
4MEDLINE
5PubMed
6CrossRef
7MEDLINE - Academic
jtitleJournal of neuropathology and experimental neurology
delivery
delcategoryRemote Search Resource
fulltextno_fulltext
addata
au
0Wei, Yu-Jia
1Guo, Wei
2Sun, Fei-Ji
3Fu, Wan-Lei
4Zheng, Da-Hai
5Chen, Xin
6Li, Song
7Zang, Zhen-Le
8Zhang, Chun-Qing
9Liu, Shi-Yong
10Yang, Hui
formatjournal
genrearticle
ristypeJOUR
atitleIncreased Expression and Cellular Localization of P2X7R in Cortical Lesions of Patients With Focal Cortical Dysplasia
jtitleJournal of neuropathology and experimental neurology
addtitleJ Neuropathol Exp Neurol
date2016-01
risdate2016
volume75
issue1
spage61
epage68
pages61-68
issn0022-3069
eissn1554-6578
abstractFocal cortical dysplasias (FCDs) are major brain malformations that commonly lead to medically intractable epilepsy. The purinergic ionotropic P2X7 receptor (P2X7R) is an atypical P2X subtype that gates calcium and sodium ions. Previous animal studies have suggested that P2X7R is a contributing factor in epileptogenesis. This study aimed to define the distribution and expression of P2X7R in 35 FCD patient-surgical-resection specimens relative to autopsy control samples (n = 8). Immunohistochemical colocalization assays revealed that P2X7R was primarily expressed in neurons, astrocytes, and microglia. In FCD samples, P2X7R protein levels were increased in abnormal cell types such as dysmorphic neurons and balloon cells, which are characteristic of FCD. By real-time PCR and Western blotting, P2X7R mRNA and protein expression levels were elevated in FCD patient samples vs control samples; P2X7R expression was also higher in FCDII vs FCDIa patient samples. Because interleukin-1β is a downstream factor of the P2X7R signaling pathway, we determined that there was also moderate-to-strong interleukin-1β expression in the dysmorphic neurons, balloon cells, and microglia in FCD patient lesions. These results indicate that increasing P2X7R levels may contribute to the pathogenesis of human FCD and that P2X7R represents a potential anti-epileptogenic target.
copEngland
pubOxford University Press
pmid26671983
doi10.1093/jnen/nlv003
oafree_for_read