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CRISPR Cas9 in Genome Editing and Beyond

The Cas9 protein (CRISPR-associated protein 9), derived from type II CRISPR (clustered regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a powerful tool for engineering the genome in diverse organisms. As an RNA-guided DNA endonuclease, Cas9 can be easily prog... Full description

Journal Title: Annual review of biochemistry 2016, Vol.85 (1), p.227-264
Main Author: Wang, Haifeng
Other Authors: La Russa, Marie , Qi, Lei S
Format: Electronic Article Electronic Article
Language: English
Subjects:
RNA
Quelle: Alma/SFX Local Collection
Publisher: United States: Annual Reviews
ID: ISSN: 0066-4154
Link: https://www.ncbi.nlm.nih.gov/pubmed/27145843
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recordid: cdi_proquest_miscellaneous_1797235375
title: CRISPR Cas9 in Genome Editing and Beyond
format: Article
creator:
  • Wang, Haifeng
  • La Russa, Marie
  • Qi, Lei S
subjects:
  • Bacterial Proteins - genetics
  • Bacterial Proteins - metabolism
  • Base Pairing
  • Biochemistry
  • Cas9 structure
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • CRISPR applications
  • CRISPR-Cas Systems
  • dCas9
  • DNA Cleavage
  • DNA Repair
  • DNA sequencing
  • Endonucleases - genetics
  • Endonucleases - metabolism
  • Epigenesis, Genetic
  • Epigenetic inheritance
  • epigenetic regulation
  • Epigenetics
  • Gene Editing - methods
  • gene regulation
  • Gene Targeting
  • Genome, Human
  • Genomes
  • genomic imaging
  • Humans
  • Immune system
  • Methods
  • Molecular Imaging
  • Nucleotide sequencing
  • Observations
  • Protein Engineering
  • Protein Structure, Secondary
  • Proteins
  • Ribonucleic acid
  • RNA
  • RNA, Guide - genetics
  • RNA, Guide - metabolism
ispartof: Annual review of biochemistry, 2016, Vol.85 (1), p.227-264
description: The Cas9 protein (CRISPR-associated protein 9), derived from type II CRISPR (clustered regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a powerful tool for engineering the genome in diverse organisms. As an RNA-guided DNA endonuclease, Cas9 can be easily programmed to target new sites by altering its guide RNA sequence, and its development as a tool has made sequence-specific gene editing several magnitudes easier. The nuclease-deactivated form of Cas9 further provides a versatile RNA-guided DNA-targeting platform for regulating and imaging the genome, as well as for rewriting the epigenetic status, all in a sequence-specific manner. With all of these advances, we have just begun to explore the possible applications of Cas9 in biomedical research and therapeutics. In this review, we describe the current models of Cas9 function and the structural and biochemical studies that support it. We focus on the applications of Cas9 for genome editing, regulation, and imaging, discuss other possible applications and some technical considerations, and highlight the many advantages that CRISPR Cas9 technology offers.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0066-4154
fulltext: fulltext
issn:
  • 0066-4154
  • 1545-4509
url: Link


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descriptionThe Cas9 protein (CRISPR-associated protein 9), derived from type II CRISPR (clustered regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a powerful tool for engineering the genome in diverse organisms. As an RNA-guided DNA endonuclease, Cas9 can be easily programmed to target new sites by altering its guide RNA sequence, and its development as a tool has made sequence-specific gene editing several magnitudes easier. The nuclease-deactivated form of Cas9 further provides a versatile RNA-guided DNA-targeting platform for regulating and imaging the genome, as well as for rewriting the epigenetic status, all in a sequence-specific manner. With all of these advances, we have just begun to explore the possible applications of Cas9 in biomedical research and therapeutics. In this review, we describe the current models of Cas9 function and the structural and biochemical studies that support it. We focus on the applications of Cas9 for genome editing, regulation, and imaging, discuss other possible applications and some technical considerations, and highlight the many advantages that CRISPR Cas9 technology offers.
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subjectBacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Base Pairing ; Biochemistry ; Cas9 structure ; Clustered Regularly Interspaced Short Palindromic Repeats ; CRISPR applications ; CRISPR-Cas Systems ; dCas9 ; DNA Cleavage ; DNA Repair ; DNA sequencing ; Endonucleases - genetics ; Endonucleases - metabolism ; Epigenesis, Genetic ; Epigenetic inheritance ; epigenetic regulation ; Epigenetics ; Gene Editing - methods ; gene regulation ; Gene Targeting ; Genome, Human ; Genomes ; genomic imaging ; Humans ; Immune system ; Methods ; Molecular Imaging ; Nucleotide sequencing ; Observations ; Protein Engineering ; Protein Structure, Secondary ; Proteins ; Ribonucleic acid ; RNA ; RNA, Guide - genetics ; RNA, Guide - metabolism
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descriptionThe Cas9 protein (CRISPR-associated protein 9), derived from type II CRISPR (clustered regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a powerful tool for engineering the genome in diverse organisms. As an RNA-guided DNA endonuclease, Cas9 can be easily programmed to target new sites by altering its guide RNA sequence, and its development as a tool has made sequence-specific gene editing several magnitudes easier. The nuclease-deactivated form of Cas9 further provides a versatile RNA-guided DNA-targeting platform for regulating and imaging the genome, as well as for rewriting the epigenetic status, all in a sequence-specific manner. With all of these advances, we have just begun to explore the possible applications of Cas9 in biomedical research and therapeutics. In this review, we describe the current models of Cas9 function and the structural and biochemical studies that support it. We focus on the applications of Cas9 for genome editing, regulation, and imaging, discuss other possible applications and some technical considerations, and highlight the many advantages that CRISPR Cas9 technology offers.
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2Base Pairing
3Biochemistry
4Cas9 structure
5Clustered Regularly Interspaced Short Palindromic Repeats
6CRISPR applications
7CRISPR-Cas Systems
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10DNA Repair
11DNA sequencing
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13Endonucleases - metabolism
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20Gene Targeting
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22Genomes
23genomic imaging
24Humans
25Immune system
26Methods
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29Observations
30Protein Engineering
31Protein Structure, Secondary
32Proteins
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34RNA
35RNA, Guide - genetics
36RNA, Guide - metabolism
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abstractThe Cas9 protein (CRISPR-associated protein 9), derived from type II CRISPR (clustered regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a powerful tool for engineering the genome in diverse organisms. As an RNA-guided DNA endonuclease, Cas9 can be easily programmed to target new sites by altering its guide RNA sequence, and its development as a tool has made sequence-specific gene editing several magnitudes easier. The nuclease-deactivated form of Cas9 further provides a versatile RNA-guided DNA-targeting platform for regulating and imaging the genome, as well as for rewriting the epigenetic status, all in a sequence-specific manner. With all of these advances, we have just begun to explore the possible applications of Cas9 in biomedical research and therapeutics. In this review, we describe the current models of Cas9 function and the structural and biochemical studies that support it. We focus on the applications of Cas9 for genome editing, regulation, and imaging, discuss other possible applications and some technical considerations, and highlight the many advantages that CRISPR Cas9 technology offers.
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