schliessen

Filtern

 

Bibliotheken

CRISPR Cas9 in Genome Editing and Beyond

The Cas9 protein (CRISPR-associated protein 9), derived from type II CRISPR (clustered regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a powerful tool for engineering the genome in diverse organisms. As an RNA-guided DNA endonuclease, Cas9 can be easily prog... Full description

Journal Title: Annual review of biochemistry 2016-06-02, Vol.85 (1), p.227-264
Main Author: Wang, Haifeng
Other Authors: La Russa, Marie , Qi, Lei S
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Alma/SFX Local Collection
Publisher: United States: Annual Reviews
ID: ISSN: 0066-4154
Link: https://www.ncbi.nlm.nih.gov/pubmed/27145843
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: cdi_proquest_miscellaneous_1797235375
title: CRISPR Cas9 in Genome Editing and Beyond
format: Article
creator:
  • Wang, Haifeng
  • La Russa, Marie
  • Qi, Lei S
subjects:
  • Bacterial Proteins - genetics
  • Bacterial Proteins - metabolism
  • Base Pairing
  • Cas9 structure
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • CRISPR applications
  • CRISPR-Cas Systems
  • dCas9
  • DNA Cleavage
  • DNA Repair
  • DNA sequencing
  • Endonucleases - genetics
  • Endonucleases - metabolism
  • Epigenesis, Genetic
  • Epigenetic inheritance
  • epigenetic regulation
  • Gene Editing - methods
  • gene regulation
  • Gene Targeting
  • Genome, Human
  • genomic imaging
  • Humans
  • Methods
  • Molecular Imaging
  • Nucleotide sequencing
  • Observations
  • Protein Engineering
  • Protein Structure, Secondary
  • RNA, Guide - genetics
  • RNA, Guide - metabolism
ispartof: Annual review of biochemistry, 2016-06-02, Vol.85 (1), p.227-264
description: The Cas9 protein (CRISPR-associated protein 9), derived from type II CRISPR (clustered regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a powerful tool for engineering the genome in diverse organisms. As an RNA-guided DNA endonuclease, Cas9 can be easily programmed to target new sites by altering its guide RNA sequence, and its development as a tool has made sequence-specific gene editing several magnitudes easier. The nuclease-deactivated form of Cas9 further provides a versatile RNA-guided DNA-targeting platform for regulating and imaging the genome, as well as for rewriting the epigenetic status, all in a sequence-specific manner. With all of these advances, we have just begun to explore the possible applications of Cas9 in biomedical research and therapeutics. In this review, we describe the current models of Cas9 function and the structural and biochemical studies that support it. We focus on the applications of Cas9 for genome editing, regulation, and imaging, discuss other possible applications and some technical considerations, and highlight the many advantages that CRISPR Cas9 technology offers.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0066-4154
fulltext: fulltext
issn:
  • 0066-4154
  • 1545-4509
url: Link


@attributes
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
RANK2.8420177
LOCALfalse
PrimoNMBib
record
control
sourceidgale_proqu
recordidTN_cdi_proquest_miscellaneous_1797235375
sourceformatXML
sourcesystemPC
galeidA455002495
sourcerecordidA455002495
originalsourceidFETCH-LOGICAL-15012-69c5772cba009595e1f124ba7ba9d9a6a7f1abe205b1d86460c17b69bf377dec3
addsrcrecordideNqVkUFP3DAQhS3UCrbQv4ByoeISGCd2vD70ABGlSEhF0J4t25mwRom9jZNW_Pt6lW0LqIciHyzNfO_Nkx4hRxROKGXVqfZ-GvBHblywK-xzqGBJeQ5pB2KHLChnPGcc5BuyAKiqnKXJHnkX4wMAlJIVu2SvEJTxJSsX5Li-vbq7uc1qHWXmfHaJPvSYXTRudP4-077JzvEx-OaAvG11F_H99t8n3z5dfK0_59dfLq_qs-uccqBFXknLhSis0QCSS460pQUzWhgtG6krLVqqDRbADW2WVQptqTCVNG0pRIO23CfHs-96CN8njKPqXbTYddpjmKKiQoqi5KXgCT2Z0XvdoXK-DeOgbXoN9s4Gj61L8zPGOUDB5EZw-A-Begp8eAKsUHfjKoZuGl3w8bnTxxm0Q4hxwFatB9fr4VFRUJue1LYnte1JzT2puae_SdaT6bH5o_5dTALqFwesG_UmR4rsuv8-cz67bDDdJdDhz_jKrL8AF727gA
sourcetypeAggregation Database
isCDItrue
recordtypearticle
pqid1797235375
display
typearticle
titleCRISPR Cas9 in Genome Editing and Beyond
sourceAlma/SFX Local Collection
creatorWang, Haifeng ; La Russa, Marie ; Qi, Lei S
creatorcontribWang, Haifeng ; La Russa, Marie ; Qi, Lei S
descriptionThe Cas9 protein (CRISPR-associated protein 9), derived from type II CRISPR (clustered regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a powerful tool for engineering the genome in diverse organisms. As an RNA-guided DNA endonuclease, Cas9 can be easily programmed to target new sites by altering its guide RNA sequence, and its development as a tool has made sequence-specific gene editing several magnitudes easier. The nuclease-deactivated form of Cas9 further provides a versatile RNA-guided DNA-targeting platform for regulating and imaging the genome, as well as for rewriting the epigenetic status, all in a sequence-specific manner. With all of these advances, we have just begun to explore the possible applications of Cas9 in biomedical research and therapeutics. In this review, we describe the current models of Cas9 function and the structural and biochemical studies that support it. We focus on the applications of Cas9 for genome editing, regulation, and imaging, discuss other possible applications and some technical considerations, and highlight the many advantages that CRISPR Cas9 technology offers.
identifier
0ISSN: 0066-4154
1EISSN: 1545-4509
2DOI: 10.1146/annurev-biochem-060815-014607
3PMID: 27145843
languageeng
publisherUnited States: Annual Reviews
subjectBacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Base Pairing ; Cas9 structure ; Clustered Regularly Interspaced Short Palindromic Repeats ; CRISPR applications ; CRISPR-Cas Systems ; dCas9 ; DNA Cleavage ; DNA Repair ; DNA sequencing ; Endonucleases - genetics ; Endonucleases - metabolism ; Epigenesis, Genetic ; Epigenetic inheritance ; epigenetic regulation ; Gene Editing - methods ; gene regulation ; Gene Targeting ; Genome, Human ; genomic imaging ; Humans ; Methods ; Molecular Imaging ; Nucleotide sequencing ; Observations ; Protein Engineering ; Protein Structure, Secondary ; RNA, Guide - genetics ; RNA, Guide - metabolism
ispartofAnnual review of biochemistry, 2016-06-02, Vol.85 (1), p.227-264
rights
0Copyright © 2016 by Annual Reviews. All rights reserved 2016
1COPYRIGHT 2016 Annual Reviews, Inc.
lds50peer_reviewed
citedbyFETCH-LOGICAL-15012-69c5772cba009595e1f124ba7ba9d9a6a7f1abe205b1d86460c17b69bf377dec3
citesFETCH-LOGICAL-15012-69c5772cba009595e1f124ba7ba9d9a6a7f1abe205b1d86460c17b69bf377dec3
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
thumbnail$$Usyndetics_thumb_exl
backlink$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27145843$$D View this record in MEDLINE/PubMed
search
creatorcontrib
0Wang, Haifeng
1La Russa, Marie
2Qi, Lei S
title
0CRISPR Cas9 in Genome Editing and Beyond
1Annual review of biochemistry
addtitleAnnu Rev Biochem
descriptionThe Cas9 protein (CRISPR-associated protein 9), derived from type II CRISPR (clustered regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a powerful tool for engineering the genome in diverse organisms. As an RNA-guided DNA endonuclease, Cas9 can be easily programmed to target new sites by altering its guide RNA sequence, and its development as a tool has made sequence-specific gene editing several magnitudes easier. The nuclease-deactivated form of Cas9 further provides a versatile RNA-guided DNA-targeting platform for regulating and imaging the genome, as well as for rewriting the epigenetic status, all in a sequence-specific manner. With all of these advances, we have just begun to explore the possible applications of Cas9 in biomedical research and therapeutics. In this review, we describe the current models of Cas9 function and the structural and biochemical studies that support it. We focus on the applications of Cas9 for genome editing, regulation, and imaging, discuss other possible applications and some technical considerations, and highlight the many advantages that CRISPR Cas9 technology offers.
subject
0Bacterial Proteins - genetics
1Bacterial Proteins - metabolism
2Base Pairing
3Cas9 structure
4Clustered Regularly Interspaced Short Palindromic Repeats
5CRISPR applications
6CRISPR-Cas Systems
7dCas9
8DNA Cleavage
9DNA Repair
10DNA sequencing
11Endonucleases - genetics
12Endonucleases - metabolism
13Epigenesis, Genetic
14Epigenetic inheritance
15epigenetic regulation
16Gene Editing - methods
17gene regulation
18Gene Targeting
19Genome, Human
20genomic imaging
21Humans
22Methods
23Molecular Imaging
24Nucleotide sequencing
25Observations
26Protein Engineering
27Protein Structure, Secondary
28RNA, Guide - genetics
29RNA, Guide - metabolism
issn
00066-4154
11545-4509
fulltexttrue
rsrctypearticle
creationdate2016
recordtypearticle
recordideNqVkUFP3DAQhS3UCrbQv4ByoeISGCd2vD70ABGlSEhF0J4t25mwRom9jZNW_Pt6lW0LqIciHyzNfO_Nkx4hRxROKGXVqfZ-GvBHblywK-xzqGBJeQ5pB2KHLChnPGcc5BuyAKiqnKXJHnkX4wMAlJIVu2SvEJTxJSsX5Li-vbq7uc1qHWXmfHaJPvSYXTRudP4-077JzvEx-OaAvG11F_H99t8n3z5dfK0_59dfLq_qs-uccqBFXknLhSis0QCSS460pQUzWhgtG6krLVqqDRbADW2WVQptqTCVNG0pRIO23CfHs-96CN8njKPqXbTYddpjmKKiQoqi5KXgCT2Z0XvdoXK-DeOgbXoN9s4Gj61L8zPGOUDB5EZw-A-Begp8eAKsUHfjKoZuGl3w8bnTxxm0Q4hxwFatB9fr4VFRUJue1LYnte1JzT2puae_SdaT6bH5o_5dTALqFwesG_UmR4rsuv8-cz67bDDdJdDhz_jKrL8AF727gA
startdate20160602
enddate20160602
creator
0Wang, Haifeng
1La Russa, Marie
2Qi, Lei S
general
0Annual Reviews
1Annual Reviews, Inc
scope
0CGR
1CUY
2CVF
3ECM
4EIF
5NPM
6AAYXX
7CITATION
8BKMMT
9BSHEE
107X8
sort
creationdate20160602
titleCRISPR Cas9 in Genome Editing and Beyond
authorWang, Haifeng ; La Russa, Marie ; Qi, Lei S
facets
frbrtype5
frbrgroupidcdi_FETCH-LOGICAL-15012-69c5772cba009595e1f124ba7ba9d9a6a7f1abe205b1d86460c17b69bf377dec3
rsrctypearticles
prefilterarticles
languageeng
creationdate2016
topic
0Bacterial Proteins - genetics
1Bacterial Proteins - metabolism
2Base Pairing
3Cas9 structure
4Clustered Regularly Interspaced Short Palindromic Repeats
5CRISPR applications
6CRISPR-Cas Systems
7dCas9
8DNA Cleavage
9DNA Repair
10DNA sequencing
11Endonucleases - genetics
12Endonucleases - metabolism
13Epigenesis, Genetic
14Epigenetic inheritance
15epigenetic regulation
16Gene Editing - methods
17gene regulation
18Gene Targeting
19Genome, Human
20genomic imaging
21Humans
22Methods
23Molecular Imaging
24Nucleotide sequencing
25Observations
26Protein Engineering
27Protein Structure, Secondary
28RNA, Guide - genetics
29RNA, Guide - metabolism
toplevel
0peer_reviewed
1online_resources
creatorcontrib
0Wang, Haifeng
1La Russa, Marie
2Qi, Lei S
collection
0Medline
1MEDLINE
2MEDLINE (Ovid)
3MEDLINE
4MEDLINE
5PubMed
6CrossRef
7Gale General OneFile (A&I only)
8Academic OneFile (A&I only)
9MEDLINE - Academic
jtitleAnnual review of biochemistry
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
au
0Wang, Haifeng
1La Russa, Marie
2Qi, Lei S
formatjournal
genrearticle
ristypeJOUR
atitleCRISPR Cas9 in Genome Editing and Beyond
jtitleAnnual review of biochemistry
addtitleAnnu Rev Biochem
date2016-06-02
risdate2016
volume85
issue1
spage227
epage264
pages227-264
issn0066-4154
eissn1545-4509
abstractThe Cas9 protein (CRISPR-associated protein 9), derived from type II CRISPR (clustered regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a powerful tool for engineering the genome in diverse organisms. As an RNA-guided DNA endonuclease, Cas9 can be easily programmed to target new sites by altering its guide RNA sequence, and its development as a tool has made sequence-specific gene editing several magnitudes easier. The nuclease-deactivated form of Cas9 further provides a versatile RNA-guided DNA-targeting platform for regulating and imaging the genome, as well as for rewriting the epigenetic status, all in a sequence-specific manner. With all of these advances, we have just begun to explore the possible applications of Cas9 in biomedical research and therapeutics. In this review, we describe the current models of Cas9 function and the structural and biochemical studies that support it. We focus on the applications of Cas9 for genome editing, regulation, and imaging, discuss other possible applications and some technical considerations, and highlight the many advantages that CRISPR Cas9 technology offers.
copUnited States
pubAnnual Reviews
pmid27145843
doi10.1146/annurev-biochem-060815-014607