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AVP dynamically increases paracellular Na+ permeability and transcellular NaCl transport in the medullary thick ascending limb of Henle’s loop

The medullary thick ascending limb of Henle’s loop (mTAL) is crucial for urine-concentrating ability of the kidney. It is water tight and able to dilute the luminal fluid by active transcellular NaCl transport, fueling the counter current mechanism by increasing interstitial osmolality. While chlori... Full description

Journal Title: Pflügers Archiv 2016, Vol.469 (1), p.149-158
Main Author: Himmerkus, Nina
Other Authors: Plain, Allein , Marques, Rita D. , Sonntag, Svenja R. , Paliege, Alexander , Leipziger, Jens , Bleich, Markus
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: Berlin/Heidelberg: Springer Berlin Heidelberg
ID: ISSN: 0031-6768
Link: https://www.ncbi.nlm.nih.gov/pubmed/27924355
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title: AVP dynamically increases paracellular Na+ permeability and transcellular NaCl transport in the medullary thick ascending limb of Henle’s loop
format: Article
creator:
  • Himmerkus, Nina
  • Plain, Allein
  • Marques, Rita D.
  • Sonntag, Svenja R.
  • Paliege, Alexander
  • Leipziger, Jens
  • Bleich, Markus
subjects:
  • Animals
  • Biological Transport - physiology
  • Biomedical and Life Sciences
  • Biomedicine
  • Cell Biology
  • Claudins
  • Concentration mechanism
  • digestive system
  • Human Physiology
  • Humans
  • Invited Review
  • Kidney Medulla - metabolism
  • Kidney Tubules - metabolism
  • Molecular Medicine
  • Neurosciences
  • Outer medulla
  • Permeability
  • Receptors
  • Sodium - metabolism
  • Sodium Chloride - metabolism
  • Sodium transport
  • Tight junction
  • tissues
  • urogenital system
  • Vasopressins - metabolism
ispartof: Pflügers Archiv, 2016, Vol.469 (1), p.149-158
description: The medullary thick ascending limb of Henle’s loop (mTAL) is crucial for urine-concentrating ability of the kidney. It is water tight and able to dilute the luminal fluid by active transcellular NaCl transport, fueling the counter current mechanism by increasing interstitial osmolality. While chloride is exclusively transported transcellularly, approx. 50% of sodium transport occurs via the paracellular route, driven by the lumen-positive transepithelial potential. Antidiuretic hormone (AVP) is known to increase active NaCl transport to support collecting duct water reabsorption. Here, we investigated the concomitant effects of AVP on the paracellular properties of mTAL. Freshly isolated mouse mTALs were perfused and electrophysiological transcellular and paracelluar properties were assessed in a paired fashion before and after AVP stimulation. In addition, the same parameters were measured in mice on a water-restricted (WR) or water-loaded (WL) diet for 5 days. Acute ex vivo stimulation as well as long-term in vivo water restriction increased equivalent short circuit current as a measure of active transcellular NaCl transport. Intriguingly, in both experimental approaches, this was accompanied by markedly increased paracellular Na + selectivity. Thus, AVP is able to acutely regulate paracellular cation selectivity in parallel to transcellular NaCl transport, allowing balanced paracellular Na + absorption under an increased transepithelial driving force.
language: eng
source:
identifier: ISSN: 0031-6768
fulltext: no_fulltext
issn:
  • 0031-6768
  • 1432-2013
url: Link


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creatorHimmerkus, Nina ; Plain, Allein ; Marques, Rita D. ; Sonntag, Svenja R. ; Paliege, Alexander ; Leipziger, Jens ; Bleich, Markus
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descriptionThe medullary thick ascending limb of Henle’s loop (mTAL) is crucial for urine-concentrating ability of the kidney. It is water tight and able to dilute the luminal fluid by active transcellular NaCl transport, fueling the counter current mechanism by increasing interstitial osmolality. While chloride is exclusively transported transcellularly, approx. 50% of sodium transport occurs via the paracellular route, driven by the lumen-positive transepithelial potential. Antidiuretic hormone (AVP) is known to increase active NaCl transport to support collecting duct water reabsorption. Here, we investigated the concomitant effects of AVP on the paracellular properties of mTAL. Freshly isolated mouse mTALs were perfused and electrophysiological transcellular and paracelluar properties were assessed in a paired fashion before and after AVP stimulation. In addition, the same parameters were measured in mice on a water-restricted (WR) or water-loaded (WL) diet for 5 days. Acute ex vivo stimulation as well as long-term in vivo water restriction increased equivalent short circuit current as a measure of active transcellular NaCl transport. Intriguingly, in both experimental approaches, this was accompanied by markedly increased paracellular Na + selectivity. Thus, AVP is able to acutely regulate paracellular cation selectivity in parallel to transcellular NaCl transport, allowing balanced paracellular Na + absorption under an increased transepithelial driving force.
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subjectAnimals ; Biological Transport - physiology ; Biomedical and Life Sciences ; Biomedicine ; Cell Biology ; Claudins ; Concentration mechanism ; digestive system ; Human Physiology ; Humans ; Invited Review ; Kidney Medulla - metabolism ; Kidney Tubules - metabolism ; Molecular Medicine ; Neurosciences ; Outer medulla ; Permeability ; Receptors ; Sodium - metabolism ; Sodium Chloride - metabolism ; Sodium transport ; Tight junction ; tissues ; urogenital system ; Vasopressins - metabolism
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descriptionThe medullary thick ascending limb of Henle’s loop (mTAL) is crucial for urine-concentrating ability of the kidney. It is water tight and able to dilute the luminal fluid by active transcellular NaCl transport, fueling the counter current mechanism by increasing interstitial osmolality. While chloride is exclusively transported transcellularly, approx. 50% of sodium transport occurs via the paracellular route, driven by the lumen-positive transepithelial potential. Antidiuretic hormone (AVP) is known to increase active NaCl transport to support collecting duct water reabsorption. Here, we investigated the concomitant effects of AVP on the paracellular properties of mTAL. Freshly isolated mouse mTALs were perfused and electrophysiological transcellular and paracelluar properties were assessed in a paired fashion before and after AVP stimulation. In addition, the same parameters were measured in mice on a water-restricted (WR) or water-loaded (WL) diet for 5 days. Acute ex vivo stimulation as well as long-term in vivo water restriction increased equivalent short circuit current as a measure of active transcellular NaCl transport. Intriguingly, in both experimental approaches, this was accompanied by markedly increased paracellular Na + selectivity. Thus, AVP is able to acutely regulate paracellular cation selectivity in parallel to transcellular NaCl transport, allowing balanced paracellular Na + absorption under an increased transepithelial driving force.
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abstractThe medullary thick ascending limb of Henle’s loop (mTAL) is crucial for urine-concentrating ability of the kidney. It is water tight and able to dilute the luminal fluid by active transcellular NaCl transport, fueling the counter current mechanism by increasing interstitial osmolality. While chloride is exclusively transported transcellularly, approx. 50% of sodium transport occurs via the paracellular route, driven by the lumen-positive transepithelial potential. Antidiuretic hormone (AVP) is known to increase active NaCl transport to support collecting duct water reabsorption. Here, we investigated the concomitant effects of AVP on the paracellular properties of mTAL. Freshly isolated mouse mTALs were perfused and electrophysiological transcellular and paracelluar properties were assessed in a paired fashion before and after AVP stimulation. In addition, the same parameters were measured in mice on a water-restricted (WR) or water-loaded (WL) diet for 5 days. Acute ex vivo stimulation as well as long-term in vivo water restriction increased equivalent short circuit current as a measure of active transcellular NaCl transport. Intriguingly, in both experimental approaches, this was accompanied by markedly increased paracellular Na + selectivity. Thus, AVP is able to acutely regulate paracellular cation selectivity in parallel to transcellular NaCl transport, allowing balanced paracellular Na + absorption under an increased transepithelial driving force.
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doi10.1007/s00424-016-1915-5
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