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Intranasal azelastine and mometasone exhibit a synergistic effect on a murine model of allergic rhinitis

Abstract Purpose The purpose of this study was to compare the anti-allergic effects of the combination of azelastine and mometasone with those of either agent alone in a Dermatophagoides farinae (Derf)-induced murine model of allergic rhinitis (AR). Materials and methods Forty BALB/c mice were divid... Full description

Journal Title: American journal of otolaryngology 2017, Vol.38 (2), p.198-203
Main Author: Kim, Do Hyun
Other Authors: Kim, Boo-Young , Shin, Ji-Hyeon , Kim, Sung Won , Kim, Soo Whan
Format: Electronic Article Electronic Article
Language: English
Subjects:
RNA
Quelle: Alma/SFX Local Collection
Publisher: United States: Elsevier Inc
ID: ISSN: 0196-0709
Link: https://www.ncbi.nlm.nih.gov/pubmed/28117118
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recordid: cdi_proquest_miscellaneous_1861607254
title: Intranasal azelastine and mometasone exhibit a synergistic effect on a murine model of allergic rhinitis
format: Article
creator:
  • Kim, Do Hyun
  • Kim, Boo-Young
  • Shin, Ji-Hyeon
  • Kim, Sung Won
  • Kim, Soo Whan
subjects:
  • Administration, Intranasal
  • Allergens
  • Allergic rhinitis
  • Allergies
  • Analysis
  • Animal models
  • Animals
  • Antihistamines
  • Asthma
  • Biological response modifiers
  • Biomarkers - analysis
  • Blotting, Western
  • Chronic illnesses
  • Coding
  • Corticosteroids
  • Cytokines
  • Dermatophagoides farinae - immunology
  • Disease
  • Disease Models, Animal
  • Drug Therapy, Combination
  • Exhibitions
  • Foxp3 protein
  • GATA-3 protein
  • Helper cells
  • Histamine
  • Hypersensitivity
  • Immunoglobulin E
  • Inflammation
  • Interferon
  • Interleukin
  • Interleukin 17
  • Interleukin 4
  • Interleukins
  • Leukocytes (eosinophilic)
  • Lymphocytes
  • Lymphocytes T
  • Mice
  • Mice, Inbred BALB C
  • Mometasone Furoate - administration & dosage
  • Mometasone Furoate - pharmacology
  • mRNA
  • Mucosa
  • Nose
  • Otolaryngology
  • Phthalazines - administration & dosage
  • Phthalazines - pharmacology
  • Polymerase chain reaction
  • Real-Time Polymerase Chain Reaction
  • Rhinitis
  • Rhinitis, Allergic - drug therapy
  • RNA
  • RNA, Messenger - analysis
  • Rubbing
  • Surgery
  • Synergistic effect
  • Western blotting
  • γ-Interferon
ispartof: American journal of otolaryngology, 2017, Vol.38 (2), p.198-203
description: Abstract Purpose The purpose of this study was to compare the anti-allergic effects of the combination of azelastine and mometasone with those of either agent alone in a Dermatophagoides farinae (Derf)-induced murine model of allergic rhinitis (AR). Materials and methods Forty BALB/c mice were divided into five groups: azelastine (A), mometasone (M), a combination of azelastine and mometasone (MA), Derf, and control. Derf served as the allergen. Allergic symptom scores, eosinophil counts, and serum Derf-specific IgE levels were measured. The mucosal levels of mRNAs encoding interferon (IFN)-γ, T-bet, interleukin (IL)-4, GATA-3, Foxp3, IL-17, and ROR-γt were determined by real-time polymerase chain reaction. The T-bet, GATA-3, Foxp3, and ROR-γt results were confirmed by Western blotting. Results Nose-rubbing motions; the levels of mRNAs encoding IL-4, GATA-3, and ROR-γt; and tissue eosinophil count were reduced in the MA compared with those in the Derf group (all P values < 0.05). The levels of mRNAs encoding GATA3 and IL-4 mRNA [synthesized by T helper (Th)2 cells] were reduced and that of mRNA encoding Foxp3 was increased in the MA compared with those in the Derf and A groups. Western blotting confirmed these findings. Conclusion We found that the combination of intranasal azelastine and mometasone synergistically suppressed Th17 responses and (reciprocally) elevated Treg responses. Therefore, this combination not only ameliorated allergic inflammation by suppressing Th2 responses, but also usefully modified the Treg/Th17 balance.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0196-0709
fulltext: fulltext
issn:
  • 0196-0709
  • 1532-818X
url: Link


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titleIntranasal azelastine and mometasone exhibit a synergistic effect on a murine model of allergic rhinitis
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creatorKim, Do Hyun ; Kim, Boo-Young ; Shin, Ji-Hyeon ; Kim, Sung Won ; Kim, Soo Whan
creatorcontribKim, Do Hyun ; Kim, Boo-Young ; Shin, Ji-Hyeon ; Kim, Sung Won ; Kim, Soo Whan
descriptionAbstract Purpose The purpose of this study was to compare the anti-allergic effects of the combination of azelastine and mometasone with those of either agent alone in a Dermatophagoides farinae (Derf)-induced murine model of allergic rhinitis (AR). Materials and methods Forty BALB/c mice were divided into five groups: azelastine (A), mometasone (M), a combination of azelastine and mometasone (MA), Derf, and control. Derf served as the allergen. Allergic symptom scores, eosinophil counts, and serum Derf-specific IgE levels were measured. The mucosal levels of mRNAs encoding interferon (IFN)-γ, T-bet, interleukin (IL)-4, GATA-3, Foxp3, IL-17, and ROR-γt were determined by real-time polymerase chain reaction. The T-bet, GATA-3, Foxp3, and ROR-γt results were confirmed by Western blotting. Results Nose-rubbing motions; the levels of mRNAs encoding IL-4, GATA-3, and ROR-γt; and tissue eosinophil count were reduced in the MA compared with those in the Derf group (all P values < 0.05). The levels of mRNAs encoding GATA3 and IL-4 mRNA [synthesized by T helper (Th)2 cells] were reduced and that of mRNA encoding Foxp3 was increased in the MA compared with those in the Derf and A groups. Western blotting confirmed these findings. Conclusion We found that the combination of intranasal azelastine and mometasone synergistically suppressed Th17 responses and (reciprocally) elevated Treg responses. Therefore, this combination not only ameliorated allergic inflammation by suppressing Th2 responses, but also usefully modified the Treg/Th17 balance.
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2DOI: 10.1016/j.amjoto.2017.01.008
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languageeng
publisherUnited States: Elsevier Inc
subjectAdministration, Intranasal ; Allergens ; Allergic rhinitis ; Allergies ; Analysis ; Animal models ; Animals ; Antihistamines ; Asthma ; Biological response modifiers ; Biomarkers - analysis ; Blotting, Western ; Chronic illnesses ; Coding ; Corticosteroids ; Cytokines ; Dermatophagoides farinae - immunology ; Disease ; Disease Models, Animal ; Drug Therapy, Combination ; Exhibitions ; Foxp3 protein ; GATA-3 protein ; Helper cells ; Histamine ; Hypersensitivity ; Immunoglobulin E ; Inflammation ; Interferon ; Interleukin ; Interleukin 17 ; Interleukin 4 ; Interleukins ; Leukocytes (eosinophilic) ; Lymphocytes ; Lymphocytes T ; Mice ; Mice, Inbred BALB C ; Mometasone Furoate - administration & dosage ; Mometasone Furoate - pharmacology ; mRNA ; Mucosa ; Nose ; Otolaryngology ; Phthalazines - administration & dosage ; Phthalazines - pharmacology ; Polymerase chain reaction ; Real-Time Polymerase Chain Reaction ; Rhinitis ; Rhinitis, Allergic - drug therapy ; RNA ; RNA, Messenger - analysis ; Rubbing ; Surgery ; Synergistic effect ; Western blotting ; γ-Interferon
ispartofAmerican journal of otolaryngology, 2017, Vol.38 (2), p.198-203
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2Shin, Ji-Hyeon
3Kim, Sung Won
4Kim, Soo Whan
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0Intranasal azelastine and mometasone exhibit a synergistic effect on a murine model of allergic rhinitis
1American journal of otolaryngology
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descriptionAbstract Purpose The purpose of this study was to compare the anti-allergic effects of the combination of azelastine and mometasone with those of either agent alone in a Dermatophagoides farinae (Derf)-induced murine model of allergic rhinitis (AR). Materials and methods Forty BALB/c mice were divided into five groups: azelastine (A), mometasone (M), a combination of azelastine and mometasone (MA), Derf, and control. Derf served as the allergen. Allergic symptom scores, eosinophil counts, and serum Derf-specific IgE levels were measured. The mucosal levels of mRNAs encoding interferon (IFN)-γ, T-bet, interleukin (IL)-4, GATA-3, Foxp3, IL-17, and ROR-γt were determined by real-time polymerase chain reaction. The T-bet, GATA-3, Foxp3, and ROR-γt results were confirmed by Western blotting. Results Nose-rubbing motions; the levels of mRNAs encoding IL-4, GATA-3, and ROR-γt; and tissue eosinophil count were reduced in the MA compared with those in the Derf group (all P values < 0.05). The levels of mRNAs encoding GATA3 and IL-4 mRNA [synthesized by T helper (Th)2 cells] were reduced and that of mRNA encoding Foxp3 was increased in the MA compared with those in the Derf and A groups. Western blotting confirmed these findings. Conclusion We found that the combination of intranasal azelastine and mometasone synergistically suppressed Th17 responses and (reciprocally) elevated Treg responses. Therefore, this combination not only ameliorated allergic inflammation by suppressing Th2 responses, but also usefully modified the Treg/Th17 balance.
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0Administration, Intranasal
1Allergens
2Allergic rhinitis
3Allergies
4Analysis
5Animal models
6Animals
7Antihistamines
8Asthma
9Biological response modifiers
10Biomarkers - analysis
11Blotting, Western
12Chronic illnesses
13Coding
14Corticosteroids
15Cytokines
16Dermatophagoides farinae - immunology
17Disease
18Disease Models, Animal
19Drug Therapy, Combination
20Exhibitions
21Foxp3 protein
22GATA-3 protein
23Helper cells
24Histamine
25Hypersensitivity
26Immunoglobulin E
27Inflammation
28Interferon
29Interleukin
30Interleukin 17
31Interleukin 4
32Interleukins
33Leukocytes (eosinophilic)
34Lymphocytes
35Lymphocytes T
36Mice
37Mice, Inbred BALB C
38Mometasone Furoate - administration & dosage
39Mometasone Furoate - pharmacology
40mRNA
41Mucosa
42Nose
43Otolaryngology
44Phthalazines - administration & dosage
45Phthalazines - pharmacology
46Polymerase chain reaction
47Real-Time Polymerase Chain Reaction
48Rhinitis
49Rhinitis, Allergic - drug therapy
50RNA
51RNA, Messenger - analysis
52Rubbing
53Surgery
54Synergistic effect
55Western blotting
56γ-Interferon
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titleIntranasal azelastine and mometasone exhibit a synergistic effect on a murine model of allergic rhinitis
authorKim, Do Hyun ; Kim, Boo-Young ; Shin, Ji-Hyeon ; Kim, Sung Won ; Kim, Soo Whan
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0Administration, Intranasal
1Allergens
2Allergic rhinitis
3Allergies
4Analysis
5Animal models
6Animals
7Antihistamines
8Asthma
9Biological response modifiers
10Biomarkers - analysis
11Blotting, Western
12Chronic illnesses
13Coding
14Corticosteroids
15Cytokines
16Dermatophagoides farinae - immunology
17Disease
18Disease Models, Animal
19Drug Therapy, Combination
20Exhibitions
21Foxp3 protein
22GATA-3 protein
23Helper cells
24Histamine
25Hypersensitivity
26Immunoglobulin E
27Inflammation
28Interferon
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31Interleukin 4
32Interleukins
33Leukocytes (eosinophilic)
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36Mice
37Mice, Inbred BALB C
38Mometasone Furoate - administration & dosage
39Mometasone Furoate - pharmacology
40mRNA
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42Nose
43Otolaryngology
44Phthalazines - administration & dosage
45Phthalazines - pharmacology
46Polymerase chain reaction
47Real-Time Polymerase Chain Reaction
48Rhinitis
49Rhinitis, Allergic - drug therapy
50RNA
51RNA, Messenger - analysis
52Rubbing
53Surgery
54Synergistic effect
55Western blotting
56γ-Interferon
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1Kim, Boo-Young
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atitleIntranasal azelastine and mometasone exhibit a synergistic effect on a murine model of allergic rhinitis
jtitleAmerican journal of otolaryngology
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date2017
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volume38
issue2
spage198
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issn0196-0709
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abstractAbstract Purpose The purpose of this study was to compare the anti-allergic effects of the combination of azelastine and mometasone with those of either agent alone in a Dermatophagoides farinae (Derf)-induced murine model of allergic rhinitis (AR). Materials and methods Forty BALB/c mice were divided into five groups: azelastine (A), mometasone (M), a combination of azelastine and mometasone (MA), Derf, and control. Derf served as the allergen. Allergic symptom scores, eosinophil counts, and serum Derf-specific IgE levels were measured. The mucosal levels of mRNAs encoding interferon (IFN)-γ, T-bet, interleukin (IL)-4, GATA-3, Foxp3, IL-17, and ROR-γt were determined by real-time polymerase chain reaction. The T-bet, GATA-3, Foxp3, and ROR-γt results were confirmed by Western blotting. Results Nose-rubbing motions; the levels of mRNAs encoding IL-4, GATA-3, and ROR-γt; and tissue eosinophil count were reduced in the MA compared with those in the Derf group (all P values < 0.05). The levels of mRNAs encoding GATA3 and IL-4 mRNA [synthesized by T helper (Th)2 cells] were reduced and that of mRNA encoding Foxp3 was increased in the MA compared with those in the Derf and A groups. Western blotting confirmed these findings. Conclusion We found that the combination of intranasal azelastine and mometasone synergistically suppressed Th17 responses and (reciprocally) elevated Treg responses. Therefore, this combination not only ameliorated allergic inflammation by suppressing Th2 responses, but also usefully modified the Treg/Th17 balance.
copUnited States
pubElsevier Inc
pmid28117118
doi10.1016/j.amjoto.2017.01.008