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Reduction of antinutritional glucosinolates in Brassica oilseeds by mutation of genes encoding transporters

The nutritional value of Brassica seed meals is reduced by the presence of glucosinolates, which are toxic compounds involved in plant defense. Mutation of the genes encoding two glucosinolate transporters (GTRs) eliminated glucosinolates from Arabidopsis thaliana seeds, but translation of loss-of-f... Full description

Journal Title: Nature biotechnology 2017-04, Vol.35 (4), p.377-382
Main Author: Nour-Eldin, Hussam Hassan
Other Authors: Madsen, Svend Roesen , Engelen, Steven , Jørgensen, Morten Egevang , Olsen, Carl Erik , Andersen, Jonathan Sonne , Seynnaeve, David , Verhoye, Thalia , Fulawka, Rudy , Denolf, Peter , Halkier, Barbara Ann
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: United States: Nature Publishing Group
ID: ISSN: 1087-0156
Link: https://www.ncbi.nlm.nih.gov/pubmed/28288105
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title: Reduction of antinutritional glucosinolates in Brassica oilseeds by mutation of genes encoding transporters
format: Article
creator:
  • Nour-Eldin, Hussam Hassan
  • Madsen, Svend Roesen
  • Engelen, Steven
  • Jørgensen, Morten Egevang
  • Olsen, Carl Erik
  • Andersen, Jonathan Sonne
  • Seynnaeve, David
  • Verhoye, Thalia
  • Fulawka, Rudy
  • Denolf, Peter
  • Halkier, Barbara Ann
subjects:
  • Arabidopsis thaliana
  • Brassica - genetics
  • Brassica juncea
  • Brassica rapa
  • Camelina sativa
  • Crambe
  • Genetic Enhancement - methods
  • Glucosinolates - analysis
  • Glucosinolates - metabolism
  • Monosaccharide Transport Proteins - genetics
  • Mutation
  • Nutritional aspects
  • Plant Oils - analysis
  • Plant Oils - chemistry
  • Plants, Genetically Modified - genetics
  • Plants, Genetically Modified - metabolism
  • Seeds - chemistry
  • Seeds - genetics
ispartof: Nature biotechnology, 2017-04, Vol.35 (4), p.377-382
description: The nutritional value of Brassica seed meals is reduced by the presence of glucosinolates, which are toxic compounds involved in plant defense. Mutation of the genes encoding two glucosinolate transporters (GTRs) eliminated glucosinolates from Arabidopsis thaliana seeds, but translation of loss-of-function phenotypes into Brassica crops is challenging because Brassica is polyploid. We mutated one of seven and four of 12 GTR orthologs and reduced glucosinolate levels in seeds by 60-70% in two different Brassica species (Brassica rapa and Brassica juncea). Reduction in seed glucosinolates was stably inherited over multiple generations and maintained in field trials of two mutant populations at three locations. Successful translation of the gtr loss-of-function phenotype from model plant to two Brassica crops suggests that our transport engineering approach could be broadly applied to reduce seed glucosinolate content in other oilseed crops, such as Camelina sativa or Crambe abyssinica.
language: eng
source:
identifier: ISSN: 1087-0156
fulltext: no_fulltext
issn:
  • 1087-0156
  • 1546-1696
url: Link


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titleReduction of antinutritional glucosinolates in Brassica oilseeds by mutation of genes encoding transporters
creatorNour-Eldin, Hussam Hassan ; Madsen, Svend Roesen ; Engelen, Steven ; Jørgensen, Morten Egevang ; Olsen, Carl Erik ; Andersen, Jonathan Sonne ; Seynnaeve, David ; Verhoye, Thalia ; Fulawka, Rudy ; Denolf, Peter ; Halkier, Barbara Ann
creatorcontribNour-Eldin, Hussam Hassan ; Madsen, Svend Roesen ; Engelen, Steven ; Jørgensen, Morten Egevang ; Olsen, Carl Erik ; Andersen, Jonathan Sonne ; Seynnaeve, David ; Verhoye, Thalia ; Fulawka, Rudy ; Denolf, Peter ; Halkier, Barbara Ann
descriptionThe nutritional value of Brassica seed meals is reduced by the presence of glucosinolates, which are toxic compounds involved in plant defense. Mutation of the genes encoding two glucosinolate transporters (GTRs) eliminated glucosinolates from Arabidopsis thaliana seeds, but translation of loss-of-function phenotypes into Brassica crops is challenging because Brassica is polyploid. We mutated one of seven and four of 12 GTR orthologs and reduced glucosinolate levels in seeds by 60-70% in two different Brassica species (Brassica rapa and Brassica juncea). Reduction in seed glucosinolates was stably inherited over multiple generations and maintained in field trials of two mutant populations at three locations. Successful translation of the gtr loss-of-function phenotype from model plant to two Brassica crops suggests that our transport engineering approach could be broadly applied to reduce seed glucosinolate content in other oilseed crops, such as Camelina sativa or Crambe abyssinica.
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subjectArabidopsis thaliana ; Brassica - genetics ; Brassica juncea ; Brassica rapa ; Camelina sativa ; Crambe ; Genetic Enhancement - methods ; Glucosinolates - analysis ; Glucosinolates - metabolism ; Monosaccharide Transport Proteins - genetics ; Mutation ; Nutritional aspects ; Plant Oils - analysis ; Plant Oils - chemistry ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - metabolism ; Seeds - chemistry ; Seeds - genetics
ispartofNature biotechnology, 2017-04, Vol.35 (4), p.377-382
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descriptionThe nutritional value of Brassica seed meals is reduced by the presence of glucosinolates, which are toxic compounds involved in plant defense. Mutation of the genes encoding two glucosinolate transporters (GTRs) eliminated glucosinolates from Arabidopsis thaliana seeds, but translation of loss-of-function phenotypes into Brassica crops is challenging because Brassica is polyploid. We mutated one of seven and four of 12 GTR orthologs and reduced glucosinolate levels in seeds by 60-70% in two different Brassica species (Brassica rapa and Brassica juncea). Reduction in seed glucosinolates was stably inherited over multiple generations and maintained in field trials of two mutant populations at three locations. Successful translation of the gtr loss-of-function phenotype from model plant to two Brassica crops suggests that our transport engineering approach could be broadly applied to reduce seed glucosinolate content in other oilseed crops, such as Camelina sativa or Crambe abyssinica.
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titleReduction of antinutritional glucosinolates in Brassica oilseeds by mutation of genes encoding transporters
authorNour-Eldin, Hussam Hassan ; Madsen, Svend Roesen ; Engelen, Steven ; Jørgensen, Morten Egevang ; Olsen, Carl Erik ; Andersen, Jonathan Sonne ; Seynnaeve, David ; Verhoye, Thalia ; Fulawka, Rudy ; Denolf, Peter ; Halkier, Barbara Ann
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7Glucosinolates - analysis
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atitleReduction of antinutritional glucosinolates in Brassica oilseeds by mutation of genes encoding transporters
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abstractThe nutritional value of Brassica seed meals is reduced by the presence of glucosinolates, which are toxic compounds involved in plant defense. Mutation of the genes encoding two glucosinolate transporters (GTRs) eliminated glucosinolates from Arabidopsis thaliana seeds, but translation of loss-of-function phenotypes into Brassica crops is challenging because Brassica is polyploid. We mutated one of seven and four of 12 GTR orthologs and reduced glucosinolate levels in seeds by 60-70% in two different Brassica species (Brassica rapa and Brassica juncea). Reduction in seed glucosinolates was stably inherited over multiple generations and maintained in field trials of two mutant populations at three locations. Successful translation of the gtr loss-of-function phenotype from model plant to two Brassica crops suggests that our transport engineering approach could be broadly applied to reduce seed glucosinolate content in other oilseed crops, such as Camelina sativa or Crambe abyssinica.
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