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Plant-made virus-like particles bearing influenza hemagglutinin (HA) recapitulate early interactions of native influenza virions with human monocytes/macrophages

•In addition to antibodies, plant-made VLP vaccines elicit an unusually strong CD4+ T cell response.•These enveloped VLPs recapitulate several key features of the initial virus-APC interactions.•Similarities of VLP/virus-cell interactions may help to explain the unusual features of the immune respon... Full description

Journal Title: Vaccine 2017, Vol.35 (35), p.4629-4636
Main Author: Makarkov, Alexander I
Other Authors: Chierzi, Sabrina , Pillet, Stéphane , Murai, Keith K , Landry, Nathalie , Ward, Brian J
Format: Electronic Article Electronic Article
Language: English
Subjects:
Acids
Analysis
Antigen processing
Antigen-presenting cells
Antigens
Avian flu
Avian influenza
Blood & organ donations
Cell fusion
Cell proliferation
Cell surface
Cholera toxin
Cloning
Compartments
Dendritic cells
Experiments
Hemagglutinins
Human monocyte-derived macrophage
Immunity
Immunogenicity
Immunological memory
Incubation
Infections
Influenza
Influenza vaccines
Influenza viruses
Internalization
Lectins
Leukocytes (mononuclear)
Lymphocytes T
Macrophages
Membranes
Microscopy
Monocytes
Peripheral blood mononuclear cells
Plant viruses
Plant-made vaccine
Proteins
Sialic acids
Stimulation
T cells
U-937 cell line
Vaccines
Virions
Virus-like particle
Virus-like particles
Viruses
Quelle: Alma/SFX Local Collection
Publisher: Netherlands: Elsevier Ltd
ID: ISSN: 0264-410X
Link: https://www.ncbi.nlm.nih.gov/pubmed/28712489
Zum Text:
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title: Plant-made virus-like particles bearing influenza hemagglutinin (HA) recapitulate early interactions of native influenza virions with human monocytes/macrophages
format: Article
creator:
  • Makarkov, Alexander I
  • Chierzi, Sabrina
  • Pillet, Stéphane
  • Murai, Keith K
  • Landry, Nathalie
  • Ward, Brian J
subjects:
  • Acids
  • Analysis
  • Antigen processing
  • Antigen-presenting cells
  • Antigens
  • Avian flu
  • Avian influenza
  • Blood & organ donations
  • Cell fusion
  • Cell proliferation
  • Cell surface
  • Cholera toxin
  • Cloning
  • Compartments
  • Dendritic cells
  • Experiments
  • Hemagglutinins
  • Human monocyte-derived macrophage
  • Immunity
  • Immunogenicity
  • Immunological memory
  • Incubation
  • Infections
  • Influenza
  • Influenza vaccines
  • Influenza viruses
  • Internalization
  • Lectins
  • Leukocytes (mononuclear)
  • Lymphocytes T
  • Macrophages
  • Membranes
  • Microscopy
  • Monocytes
  • Peripheral blood mononuclear cells
  • Plant viruses
  • Plant-made vaccine
  • Proteins
  • Sialic acids
  • Stimulation
  • T cells
  • U-937 cell line
  • Vaccines
  • Virions
  • Virus-like particle
  • Virus-like particles
  • Viruses
ispartof: Vaccine, 2017, Vol.35 (35), p.4629-4636
description: •In addition to antibodies, plant-made VLP vaccines elicit an unusually strong CD4+ T cell response.•These enveloped VLPs recapitulate several key features of the initial virus-APC interactions.•Similarities of VLP/virus-cell interactions may help to explain the unusual features of the immune response induced. Plant-made virus-like particles (VLP) bearing influenza virus hemagglutinins (HA) are novel vaccine candidates that induce cross-reactive humoral and poly-functional T cell responses. To better understand the mechanisms that underlie this broad immunogenicity we studied early interactions of VLPs bearing either H1 (A/California/07/2009 (H1N1)) or H5 (A/Indonesia/05/2005 (H5N1)) with a human monocytoid cell line (U-937 cells) and human monocyte-derived macrophages (MDMs) as model antigen-presenting cells (APC). Using Vibrio cholerae sialidase and lectins that target α2,6- (Sambucus nigra lectin) or α2,3-linked sialic acids (Maackia amurensis lectin I), we demonstrated that VLPs bind to these APCs in a sialic acid-dependent manner. Using lysosomal markers and DiD-labelled VLPs, we found that attachment to the cell surface leads to internalization, trafficking to acidic cell compartments and fusion of the VLP lipid envelope with endosomal membranes. Incubation of MDMs with H1- but not H5-VLPs induced proliferation of autologous peripheral blood mononuclear cells suggesting antigen processing and stimulation of a memory T cell response. Plant-made VLPs bearing influenza HA not only mimic the structure of influenza virions to some degree but also recapitulate key features of the initial virus-APC interaction. These observations may help to explain the balanced humoral and cellular responses to plant-made VLP vaccines.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0264-410X
fulltext: fulltext
issn:
  • 0264-410X
  • 1873-2518
url: Link


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titlePlant-made virus-like particles bearing influenza hemagglutinin (HA) recapitulate early interactions of native influenza virions with human monocytes/macrophages
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creatorMakarkov, Alexander I ; Chierzi, Sabrina ; Pillet, Stéphane ; Murai, Keith K ; Landry, Nathalie ; Ward, Brian J
creatorcontribMakarkov, Alexander I ; Chierzi, Sabrina ; Pillet, Stéphane ; Murai, Keith K ; Landry, Nathalie ; Ward, Brian J
description•In addition to antibodies, plant-made VLP vaccines elicit an unusually strong CD4+ T cell response.•These enveloped VLPs recapitulate several key features of the initial virus-APC interactions.•Similarities of VLP/virus-cell interactions may help to explain the unusual features of the immune response induced. Plant-made virus-like particles (VLP) bearing influenza virus hemagglutinins (HA) are novel vaccine candidates that induce cross-reactive humoral and poly-functional T cell responses. To better understand the mechanisms that underlie this broad immunogenicity we studied early interactions of VLPs bearing either H1 (A/California/07/2009 (H1N1)) or H5 (A/Indonesia/05/2005 (H5N1)) with a human monocytoid cell line (U-937 cells) and human monocyte-derived macrophages (MDMs) as model antigen-presenting cells (APC). Using Vibrio cholerae sialidase and lectins that target α2,6- (Sambucus nigra lectin) or α2,3-linked sialic acids (Maackia amurensis lectin I), we demonstrated that VLPs bind to these APCs in a sialic acid-dependent manner. Using lysosomal markers and DiD-labelled VLPs, we found that attachment to the cell surface leads to internalization, trafficking to acidic cell compartments and fusion of the VLP lipid envelope with endosomal membranes. Incubation of MDMs with H1- but not H5-VLPs induced proliferation of autologous peripheral blood mononuclear cells suggesting antigen processing and stimulation of a memory T cell response. Plant-made VLPs bearing influenza HA not only mimic the structure of influenza virions to some degree but also recapitulate key features of the initial virus-APC interaction. These observations may help to explain the balanced humoral and cellular responses to plant-made VLP vaccines.
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languageeng
publisherNetherlands: Elsevier Ltd
subjectAcids ; Analysis ; Antigen processing ; Antigen-presenting cells ; Antigens ; Avian flu ; Avian influenza ; Blood & organ donations ; Cell fusion ; Cell proliferation ; Cell surface ; Cholera toxin ; Cloning ; Compartments ; Dendritic cells ; Experiments ; Hemagglutinins ; Human monocyte-derived macrophage ; Immunity ; Immunogenicity ; Immunological memory ; Incubation ; Infections ; Influenza ; Influenza vaccines ; Influenza viruses ; Internalization ; Lectins ; Leukocytes (mononuclear) ; Lymphocytes T ; Macrophages ; Membranes ; Microscopy ; Monocytes ; Peripheral blood mononuclear cells ; Plant viruses ; Plant-made vaccine ; Proteins ; Sialic acids ; Stimulation ; T cells ; U-937 cell line ; Vaccines ; Virions ; Virus-like particle ; Virus-like particles ; Viruses
ispartofVaccine, 2017, Vol.35 (35), p.4629-4636
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description•In addition to antibodies, plant-made VLP vaccines elicit an unusually strong CD4+ T cell response.•These enveloped VLPs recapitulate several key features of the initial virus-APC interactions.•Similarities of VLP/virus-cell interactions may help to explain the unusual features of the immune response induced. Plant-made virus-like particles (VLP) bearing influenza virus hemagglutinins (HA) are novel vaccine candidates that induce cross-reactive humoral and poly-functional T cell responses. To better understand the mechanisms that underlie this broad immunogenicity we studied early interactions of VLPs bearing either H1 (A/California/07/2009 (H1N1)) or H5 (A/Indonesia/05/2005 (H5N1)) with a human monocytoid cell line (U-937 cells) and human monocyte-derived macrophages (MDMs) as model antigen-presenting cells (APC). Using Vibrio cholerae sialidase and lectins that target α2,6- (Sambucus nigra lectin) or α2,3-linked sialic acids (Maackia amurensis lectin I), we demonstrated that VLPs bind to these APCs in a sialic acid-dependent manner. Using lysosomal markers and DiD-labelled VLPs, we found that attachment to the cell surface leads to internalization, trafficking to acidic cell compartments and fusion of the VLP lipid envelope with endosomal membranes. Incubation of MDMs with H1- but not H5-VLPs induced proliferation of autologous peripheral blood mononuclear cells suggesting antigen processing and stimulation of a memory T cell response. Plant-made VLPs bearing influenza HA not only mimic the structure of influenza virions to some degree but also recapitulate key features of the initial virus-APC interaction. These observations may help to explain the balanced humoral and cellular responses to plant-made VLP vaccines.
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5Avian flu
6Avian influenza
7Blood & organ donations
8Cell fusion
9Cell proliferation
10Cell surface
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12Cloning
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15Experiments
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17Human monocyte-derived macrophage
18Immunity
19Immunogenicity
20Immunological memory
21Incubation
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24Influenza vaccines
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28Leukocytes (mononuclear)
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titlePlant-made virus-like particles bearing influenza hemagglutinin (HA) recapitulate early interactions of native influenza virions with human monocytes/macrophages
authorMakarkov, Alexander I ; Chierzi, Sabrina ; Pillet, Stéphane ; Murai, Keith K ; Landry, Nathalie ; Ward, Brian J
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7Blood & organ donations
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9Cell proliferation
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atitlePlant-made virus-like particles bearing influenza hemagglutinin (HA) recapitulate early interactions of native influenza virions with human monocytes/macrophages
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issn0264-410X
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abstract•In addition to antibodies, plant-made VLP vaccines elicit an unusually strong CD4+ T cell response.•These enveloped VLPs recapitulate several key features of the initial virus-APC interactions.•Similarities of VLP/virus-cell interactions may help to explain the unusual features of the immune response induced. Plant-made virus-like particles (VLP) bearing influenza virus hemagglutinins (HA) are novel vaccine candidates that induce cross-reactive humoral and poly-functional T cell responses. To better understand the mechanisms that underlie this broad immunogenicity we studied early interactions of VLPs bearing either H1 (A/California/07/2009 (H1N1)) or H5 (A/Indonesia/05/2005 (H5N1)) with a human monocytoid cell line (U-937 cells) and human monocyte-derived macrophages (MDMs) as model antigen-presenting cells (APC). Using Vibrio cholerae sialidase and lectins that target α2,6- (Sambucus nigra lectin) or α2,3-linked sialic acids (Maackia amurensis lectin I), we demonstrated that VLPs bind to these APCs in a sialic acid-dependent manner. Using lysosomal markers and DiD-labelled VLPs, we found that attachment to the cell surface leads to internalization, trafficking to acidic cell compartments and fusion of the VLP lipid envelope with endosomal membranes. Incubation of MDMs with H1- but not H5-VLPs induced proliferation of autologous peripheral blood mononuclear cells suggesting antigen processing and stimulation of a memory T cell response. Plant-made VLPs bearing influenza HA not only mimic the structure of influenza virions to some degree but also recapitulate key features of the initial virus-APC interaction. These observations may help to explain the balanced humoral and cellular responses to plant-made VLP vaccines.
copNetherlands
pubElsevier Ltd
pmid28712489
doi10.1016/j.vaccine.2017.07.012