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Efficient determination of six fatty acid ethyl ethers in human whole blood by gas chromatography-mass spectrometry

Abstract Fatty acid ethyl esters (FAEEs) have been widely studied as specific markers of ethanol intake and mediators of ethanol-induced diseases. In the present study, a simple and rapid gas chromatography-mass spectrometry (GC-MS) method was established for the qualitative and quantitative analysi... Full description

Journal Title: Alcohol (Fayetteville N.Y.), 2017, Vol.62, p.41-47
Main Author: Li, Jiaolun
Other Authors: Zhang, Xinyu , Lin, Zebin , Huang, Zhibin , Rao, Yulan
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Alma/SFX Local Collection
Publisher: United States: Elsevier Inc
ID: ISSN: 0741-8329
Link: https://www.ncbi.nlm.nih.gov/pubmed/28755750
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title: Efficient determination of six fatty acid ethyl ethers in human whole blood by gas chromatography-mass spectrometry
format: Article
creator:
  • Li, Jiaolun
  • Zhang, Xinyu
  • Lin, Zebin
  • Huang, Zhibin
  • Rao, Yulan
subjects:
  • Adult
  • Alcohol
  • Alcohol Drinking - blood
  • Alcohol use
  • Analysis
  • Biomarkers - analysis
  • Blood
  • Chemistry
  • China
  • Chromatography
  • Esters
  • Ethanol
  • Ethanol - blood
  • Ethanol markers
  • Ethers
  • Ethyl esters
  • Ethyl Ethers - blood
  • Fatty acid ethyl esters
  • Fatty acids
  • Fatty Acids - blood
  • Female
  • Forensic engineering
  • Forensic science
  • Forensic Sciences
  • Gas chromatography
  • Gas chromatography-mass spectrometry
  • Gas Chromatography-Mass Spectrometry - methods
  • Human whole blood
  • Humans
  • Limit of Detection
  • Linearity
  • Liquid-liquid extraction
  • Male
  • Mass spectrometry
  • Mass spectroscopy
  • Metabolites
  • Middle Aged
  • Palmitic acid
  • Plasma
  • Psychiatry
  • Qualitative analysis
  • Quantitative analysis
  • Reproducibility of Results
  • Scientific imaging
  • Sensitivity and Specificity
ispartof: Alcohol (Fayetteville, N.Y.), 2017, Vol.62, p.41-47
description: Abstract Fatty acid ethyl esters (FAEEs) have been widely studied as specific markers of ethanol intake and mediators of ethanol-induced diseases. In the present study, a simple and rapid gas chromatography-mass spectrometry (GC-MS) method was established for the qualitative and quantitative analysis of six fatty acid ethyl esters (FAEEs), including ethyl myristate, ethyl palmitate, ethyl stearate, ethyl oleate, ethyl linoleate, and ethyl arachidonate, in human whole blood. FAEEs were extracted from 200 μL of human whole blood by a modified liquid-liquid extraction, and the hexane layer was injected directly into GC-MS with ethyl heptadecanoate as the internal standard. The limits of detection (LODs) and limits of quantification (LOQs) were in the range of 5–50 ng/mL and 15–200 ng/mL, respectively. Linearity ranged up to 10 μg/mL with r2 higher than 0.998. Accuracy was in the range of 90.3–109.7%, while intra-day and inter-day precision were 0.7–9.3% and 3.4–12.5%, respectively. This method was then applied to 38 real samples from forensic cases. Differences in the most common FAEEs between Chinese and Western subjects were discussed. The relationship of FAEE concentrations with age and gender was also investigated.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0741-8329
fulltext: fulltext
issn:
  • 0741-8329
  • 1873-6823
url: Link


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descriptionAbstract Fatty acid ethyl esters (FAEEs) have been widely studied as specific markers of ethanol intake and mediators of ethanol-induced diseases. In the present study, a simple and rapid gas chromatography-mass spectrometry (GC-MS) method was established for the qualitative and quantitative analysis of six fatty acid ethyl esters (FAEEs), including ethyl myristate, ethyl palmitate, ethyl stearate, ethyl oleate, ethyl linoleate, and ethyl arachidonate, in human whole blood. FAEEs were extracted from 200 μL of human whole blood by a modified liquid-liquid extraction, and the hexane layer was injected directly into GC-MS with ethyl heptadecanoate as the internal standard. The limits of detection (LODs) and limits of quantification (LOQs) were in the range of 5–50 ng/mL and 15–200 ng/mL, respectively. Linearity ranged up to 10 μg/mL with r2 higher than 0.998. Accuracy was in the range of 90.3–109.7%, while intra-day and inter-day precision were 0.7–9.3% and 3.4–12.5%, respectively. This method was then applied to 38 real samples from forensic cases. Differences in the most common FAEEs between Chinese and Western subjects were discussed. The relationship of FAEE concentrations with age and gender was also investigated.
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subjectAdult ; Alcohol ; Alcohol Drinking - blood ; Alcohol use ; Analysis ; Biomarkers - analysis ; Blood ; Chemistry ; China ; Chromatography ; Esters ; Ethanol ; Ethanol - blood ; Ethanol markers ; Ethers ; Ethyl esters ; Ethyl Ethers - blood ; Fatty acid ethyl esters ; Fatty acids ; Fatty Acids - blood ; Female ; Forensic engineering ; Forensic science ; Forensic Sciences ; Gas chromatography ; Gas chromatography-mass spectrometry ; Gas Chromatography-Mass Spectrometry - methods ; Human whole blood ; Humans ; Limit of Detection ; Linearity ; Liquid-liquid extraction ; Male ; Mass spectrometry ; Mass spectroscopy ; Metabolites ; Middle Aged ; Palmitic acid ; Plasma ; Psychiatry ; Qualitative analysis ; Quantitative analysis ; Reproducibility of Results ; Scientific imaging ; Sensitivity and Specificity
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descriptionAbstract Fatty acid ethyl esters (FAEEs) have been widely studied as specific markers of ethanol intake and mediators of ethanol-induced diseases. In the present study, a simple and rapid gas chromatography-mass spectrometry (GC-MS) method was established for the qualitative and quantitative analysis of six fatty acid ethyl esters (FAEEs), including ethyl myristate, ethyl palmitate, ethyl stearate, ethyl oleate, ethyl linoleate, and ethyl arachidonate, in human whole blood. FAEEs were extracted from 200 μL of human whole blood by a modified liquid-liquid extraction, and the hexane layer was injected directly into GC-MS with ethyl heptadecanoate as the internal standard. The limits of detection (LODs) and limits of quantification (LOQs) were in the range of 5–50 ng/mL and 15–200 ng/mL, respectively. Linearity ranged up to 10 μg/mL with r2 higher than 0.998. Accuracy was in the range of 90.3–109.7%, while intra-day and inter-day precision were 0.7–9.3% and 3.4–12.5%, respectively. This method was then applied to 38 real samples from forensic cases. Differences in the most common FAEEs between Chinese and Western subjects were discussed. The relationship of FAEE concentrations with age and gender was also investigated.
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abstractAbstract Fatty acid ethyl esters (FAEEs) have been widely studied as specific markers of ethanol intake and mediators of ethanol-induced diseases. In the present study, a simple and rapid gas chromatography-mass spectrometry (GC-MS) method was established for the qualitative and quantitative analysis of six fatty acid ethyl esters (FAEEs), including ethyl myristate, ethyl palmitate, ethyl stearate, ethyl oleate, ethyl linoleate, and ethyl arachidonate, in human whole blood. FAEEs were extracted from 200 μL of human whole blood by a modified liquid-liquid extraction, and the hexane layer was injected directly into GC-MS with ethyl heptadecanoate as the internal standard. The limits of detection (LODs) and limits of quantification (LOQs) were in the range of 5–50 ng/mL and 15–200 ng/mL, respectively. Linearity ranged up to 10 μg/mL with r2 higher than 0.998. Accuracy was in the range of 90.3–109.7%, while intra-day and inter-day precision were 0.7–9.3% and 3.4–12.5%, respectively. This method was then applied to 38 real samples from forensic cases. Differences in the most common FAEEs between Chinese and Western subjects were discussed. The relationship of FAEE concentrations with age and gender was also investigated.
copUnited States
pubElsevier Inc
pmid28755750
doi10.1016/j.alcohol.2017.02.359