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The maize WRKY transcription factor ZmWRKY17 negatively regulates salt stress tolerance in transgenic Arabidopsis plants

Main conclusion We cloned and characterized the ZmWRKY17 gene from maize. Overexpression of ZmWRKY17 in Arabidopsis led to increased sensitivity to salt stress and decreased ABA sensitivity through regulating the expression of some ABA- and stress-responsive genes. The WRKY transcription factors hav... Full description

Journal Title: Planta 2017-08-31, Vol.246 (6), p.1215-1231
Main Author: Cai, Ronghao
Other Authors: Dai, Wei , Zhang, Congsheng , Wang, Yan , Wu, Min , Zhao, Yang , Ma, Qing , Xiang, Yan , Cheng, Beijiu
Format: Electronic Article Electronic Article
Language: English
Subjects:
RNA
Publisher: Berlin/Heidelberg: Springer Berlin Heidelberg
ID: ISSN: 0032-0935
Link: https://www.ncbi.nlm.nih.gov/pubmed/28861611
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recordid: cdi_proquest_miscellaneous_1936158191
title: The maize WRKY transcription factor ZmWRKY17 negatively regulates salt stress tolerance in transgenic Arabidopsis plants
format: Article
creator:
  • Cai, Ronghao
  • Dai, Wei
  • Zhang, Congsheng
  • Wang, Yan
  • Wu, Min
  • Zhao, Yang
  • Ma, Qing
  • Xiang, Yan
  • Cheng, Beijiu
subjects:
  • Abiotic stress
  • Abscisic acid
  • Abscisic Acid - analysis
  • Agriculture
  • Amino Acid Sequence
  • Analysis
  • Arabidopsis
  • Arabidopsis - genetics
  • Arabidopsis - physiology
  • Arabidopsis thaliana
  • Biological activity
  • Biomedical and Life Sciences
  • Chromosome Mapping
  • Corn
  • Cotyledon - genetics
  • Cotyledon - physiology
  • Cotyledons
  • DNA binding proteins
  • Drought
  • Droughts
  • Ecology
  • Forestry
  • Gene expression
  • Gene Expression Regulation, Plant
  • Genes
  • Genetic engineering
  • Genetic research
  • Genetically modified plants
  • Germination
  • Greening
  • Life Sciences
  • Malondialdehyde
  • Nuclei
  • Original Article
  • Phenotype
  • Phylogeny
  • Physiological aspects
  • Plant Growth Regulators - analysis
  • Plant Proteins - genetics
  • Plant Proteins - metabolism
  • Plant Roots - genetics
  • Plant Roots - physiology
  • Plant Sciences
  • Plants
  • Plants (botany)
  • Plants, Genetically Modified
  • Regulators
  • RNA
  • Salinity tolerance
  • Salt Tolerance
  • Salts
  • Seed germination
  • Seedlings
  • Seedlings - genetics
  • Seedlings - physiology
  • Sensitivity
  • Sequence Alignment
  • Sequence Analysis, RNA
  • Signaling
  • Sodium chloride
  • Stress
  • Stress, Physiological
  • Stresses
  • Transcription activation
  • Transcription factors
  • Transcription Factors - genetics
  • Transcription Factors - metabolism
  • Transgenic plants
  • Yeast
  • Zea mays - genetics
  • Zea mays - physiology
ispartof: Planta, 2017-08-31, Vol.246 (6), p.1215-1231
description: Main conclusion We cloned and characterized the ZmWRKY17 gene from maize. Overexpression of ZmWRKY17 in Arabidopsis led to increased sensitivity to salt stress and decreased ABA sensitivity through regulating the expression of some ABA- and stress-responsive genes. The WRKY transcription factors have been reported to function as positive or negative regulators in many different biological processes including plant development, defense regulation and stress response. This study isolated a maize WRKY gene, ZmWRKY17 , and characterized its role in tolerance to salt stress by generating transgenic Arabidopsis plants. Expression of the ZmWRKY17 was up-regulated by drought, salt and abscisic acid (ABA) treatments. ZmWRKY17 was localized in the nucleus with no transcriptional activation in yeast. Yeast one-hybrid assay showed that ZmWRKY17 can specifically bind to W-box, and it can activate W-box-dependent transcription in planta. Heterologous overexpression of ZmWRKY17 in Arabidopsis remarkably reduced plant tolerance to salt stress, as determined through physiological analyses of the cotyledons greening rate, root growth, relative electrical leakage and malondialdehyde content. Additionally, ZmWRKY17 transgenic plants showed decreased sensitivity to ABA during seed germination and early seedling growth. Transgenic plants accumulated higher content of ABA than wild-type (WT) plants under NaCl condition. Transcriptome and quantitative real-time PCR analyses revealed that some stress-related genes in transgenic seedlings showed lower expression level than that in the WT when treated with NaCl. Taken together, these results suggest that ZmWRKY17 may act as a negative regulator involved in the salt stress responses through ABA signalling.
language: eng
source:
identifier: ISSN: 0032-0935
fulltext: no_fulltext
issn:
  • 0032-0935
  • 1432-2048
url: Link


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titleThe maize WRKY transcription factor ZmWRKY17 negatively regulates salt stress tolerance in transgenic Arabidopsis plants
creatorCai, Ronghao ; Dai, Wei ; Zhang, Congsheng ; Wang, Yan ; Wu, Min ; Zhao, Yang ; Ma, Qing ; Xiang, Yan ; Cheng, Beijiu
creatorcontribCai, Ronghao ; Dai, Wei ; Zhang, Congsheng ; Wang, Yan ; Wu, Min ; Zhao, Yang ; Ma, Qing ; Xiang, Yan ; Cheng, Beijiu
descriptionMain conclusion We cloned and characterized the ZmWRKY17 gene from maize. Overexpression of ZmWRKY17 in Arabidopsis led to increased sensitivity to salt stress and decreased ABA sensitivity through regulating the expression of some ABA- and stress-responsive genes. The WRKY transcription factors have been reported to function as positive or negative regulators in many different biological processes including plant development, defense regulation and stress response. This study isolated a maize WRKY gene, ZmWRKY17 , and characterized its role in tolerance to salt stress by generating transgenic Arabidopsis plants. Expression of the ZmWRKY17 was up-regulated by drought, salt and abscisic acid (ABA) treatments. ZmWRKY17 was localized in the nucleus with no transcriptional activation in yeast. Yeast one-hybrid assay showed that ZmWRKY17 can specifically bind to W-box, and it can activate W-box-dependent transcription in planta. Heterologous overexpression of ZmWRKY17 in Arabidopsis remarkably reduced plant tolerance to salt stress, as determined through physiological analyses of the cotyledons greening rate, root growth, relative electrical leakage and malondialdehyde content. Additionally, ZmWRKY17 transgenic plants showed decreased sensitivity to ABA during seed germination and early seedling growth. Transgenic plants accumulated higher content of ABA than wild-type (WT) plants under NaCl condition. Transcriptome and quantitative real-time PCR analyses revealed that some stress-related genes in transgenic seedlings showed lower expression level than that in the WT when treated with NaCl. Taken together, these results suggest that ZmWRKY17 may act as a negative regulator involved in the salt stress responses through ABA signalling.
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1EISSN: 1432-2048
2DOI: 10.1007/s00425-017-2766-9
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languageeng
publisherBerlin/Heidelberg: Springer Berlin Heidelberg
subjectAbiotic stress ; Abscisic acid ; Abscisic Acid - analysis ; Agriculture ; Amino Acid Sequence ; Analysis ; Arabidopsis ; Arabidopsis - genetics ; Arabidopsis - physiology ; Arabidopsis thaliana ; Biological activity ; Biomedical and Life Sciences ; Chromosome Mapping ; Corn ; Cotyledon - genetics ; Cotyledon - physiology ; Cotyledons ; DNA binding proteins ; Drought ; Droughts ; Ecology ; Forestry ; Gene expression ; Gene Expression Regulation, Plant ; Genes ; Genetic engineering ; Genetic research ; Genetically modified plants ; Germination ; Greening ; Life Sciences ; Malondialdehyde ; Nuclei ; Original Article ; Phenotype ; Phylogeny ; Physiological aspects ; Plant Growth Regulators - analysis ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Roots - genetics ; Plant Roots - physiology ; Plant Sciences ; Plants ; Plants (botany) ; Plants, Genetically Modified ; Regulators ; RNA ; Salinity tolerance ; Salt Tolerance ; Salts ; Seed germination ; Seedlings ; Seedlings - genetics ; Seedlings - physiology ; Sensitivity ; Sequence Alignment ; Sequence Analysis, RNA ; Signaling ; Sodium chloride ; Stress ; Stress, Physiological ; Stresses ; Transcription activation ; Transcription factors ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transgenic plants ; Yeast ; Zea mays - genetics ; Zea mays - physiology
ispartofPlanta, 2017-08-31, Vol.246 (6), p.1215-1231
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0Springer-Verlag GmbH Germany 2017
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2Planta is a copyright of Springer, 2017.
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3Wang, Yan
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6Ma, Qing
7Xiang, Yan
8Cheng, Beijiu
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descriptionMain conclusion We cloned and characterized the ZmWRKY17 gene from maize. Overexpression of ZmWRKY17 in Arabidopsis led to increased sensitivity to salt stress and decreased ABA sensitivity through regulating the expression of some ABA- and stress-responsive genes. The WRKY transcription factors have been reported to function as positive or negative regulators in many different biological processes including plant development, defense regulation and stress response. This study isolated a maize WRKY gene, ZmWRKY17 , and characterized its role in tolerance to salt stress by generating transgenic Arabidopsis plants. Expression of the ZmWRKY17 was up-regulated by drought, salt and abscisic acid (ABA) treatments. ZmWRKY17 was localized in the nucleus with no transcriptional activation in yeast. Yeast one-hybrid assay showed that ZmWRKY17 can specifically bind to W-box, and it can activate W-box-dependent transcription in planta. Heterologous overexpression of ZmWRKY17 in Arabidopsis remarkably reduced plant tolerance to salt stress, as determined through physiological analyses of the cotyledons greening rate, root growth, relative electrical leakage and malondialdehyde content. Additionally, ZmWRKY17 transgenic plants showed decreased sensitivity to ABA during seed germination and early seedling growth. Transgenic plants accumulated higher content of ABA than wild-type (WT) plants under NaCl condition. Transcriptome and quantitative real-time PCR analyses revealed that some stress-related genes in transgenic seedlings showed lower expression level than that in the WT when treated with NaCl. Taken together, these results suggest that ZmWRKY17 may act as a negative regulator involved in the salt stress responses through ABA signalling.
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0Abiotic stress
1Abscisic acid
2Abscisic Acid - analysis
3Agriculture
4Amino Acid Sequence
5Analysis
6Arabidopsis
7Arabidopsis - genetics
8Arabidopsis - physiology
9Arabidopsis thaliana
10Biological activity
11Biomedical and Life Sciences
12Chromosome Mapping
13Corn
14Cotyledon - genetics
15Cotyledon - physiology
16Cotyledons
17DNA binding proteins
18Drought
19Droughts
20Ecology
21Forestry
22Gene expression
23Gene Expression Regulation, Plant
24Genes
25Genetic engineering
26Genetic research
27Genetically modified plants
28Germination
29Greening
30Life Sciences
31Malondialdehyde
32Nuclei
33Original Article
34Phenotype
35Phylogeny
36Physiological aspects
37Plant Growth Regulators - analysis
38Plant Proteins - genetics
39Plant Proteins - metabolism
40Plant Roots - genetics
41Plant Roots - physiology
42Plant Sciences
43Plants
44Plants (botany)
45Plants, Genetically Modified
46Regulators
47RNA
48Salinity tolerance
49Salt Tolerance
50Salts
51Seed germination
52Seedlings
53Seedlings - genetics
54Seedlings - physiology
55Sensitivity
56Sequence Alignment
57Sequence Analysis, RNA
58Signaling
59Sodium chloride
60Stress
61Stress, Physiological
62Stresses
63Transcription activation
64Transcription factors
65Transcription Factors - genetics
66Transcription Factors - metabolism
67Transgenic plants
68Yeast
69Zea mays - genetics
70Zea mays - physiology
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titleThe maize WRKY transcription factor ZmWRKY17 negatively regulates salt stress tolerance in transgenic Arabidopsis plants
authorCai, Ronghao ; Dai, Wei ; Zhang, Congsheng ; Wang, Yan ; Wu, Min ; Zhao, Yang ; Ma, Qing ; Xiang, Yan ; Cheng, Beijiu
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1Abscisic acid
2Abscisic Acid - analysis
3Agriculture
4Amino Acid Sequence
5Analysis
6Arabidopsis
7Arabidopsis - genetics
8Arabidopsis - physiology
9Arabidopsis thaliana
10Biological activity
11Biomedical and Life Sciences
12Chromosome Mapping
13Corn
14Cotyledon - genetics
15Cotyledon - physiology
16Cotyledons
17DNA binding proteins
18Drought
19Droughts
20Ecology
21Forestry
22Gene expression
23Gene Expression Regulation, Plant
24Genes
25Genetic engineering
26Genetic research
27Genetically modified plants
28Germination
29Greening
30Life Sciences
31Malondialdehyde
32Nuclei
33Original Article
34Phenotype
35Phylogeny
36Physiological aspects
37Plant Growth Regulators - analysis
38Plant Proteins - genetics
39Plant Proteins - metabolism
40Plant Roots - genetics
41Plant Roots - physiology
42Plant Sciences
43Plants
44Plants (botany)
45Plants, Genetically Modified
46Regulators
47RNA
48Salinity tolerance
49Salt Tolerance
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52Seedlings
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54Seedlings - physiology
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62Stresses
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66Transcription Factors - metabolism
67Transgenic plants
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69Zea mays - genetics
70Zea mays - physiology
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abstractMain conclusion We cloned and characterized the ZmWRKY17 gene from maize. Overexpression of ZmWRKY17 in Arabidopsis led to increased sensitivity to salt stress and decreased ABA sensitivity through regulating the expression of some ABA- and stress-responsive genes. The WRKY transcription factors have been reported to function as positive or negative regulators in many different biological processes including plant development, defense regulation and stress response. This study isolated a maize WRKY gene, ZmWRKY17 , and characterized its role in tolerance to salt stress by generating transgenic Arabidopsis plants. Expression of the ZmWRKY17 was up-regulated by drought, salt and abscisic acid (ABA) treatments. ZmWRKY17 was localized in the nucleus with no transcriptional activation in yeast. Yeast one-hybrid assay showed that ZmWRKY17 can specifically bind to W-box, and it can activate W-box-dependent transcription in planta. Heterologous overexpression of ZmWRKY17 in Arabidopsis remarkably reduced plant tolerance to salt stress, as determined through physiological analyses of the cotyledons greening rate, root growth, relative electrical leakage and malondialdehyde content. Additionally, ZmWRKY17 transgenic plants showed decreased sensitivity to ABA during seed germination and early seedling growth. Transgenic plants accumulated higher content of ABA than wild-type (WT) plants under NaCl condition. Transcriptome and quantitative real-time PCR analyses revealed that some stress-related genes in transgenic seedlings showed lower expression level than that in the WT when treated with NaCl. Taken together, these results suggest that ZmWRKY17 may act as a negative regulator involved in the salt stress responses through ABA signalling.
copBerlin/Heidelberg
pubSpringer Berlin Heidelberg
pmid28861611
doi10.1007/s00425-017-2766-9