schliessen

Filtern

 

Bibliotheken

Validation of an in vitro system for studies of pathogenicity mechanisms in Xanthomonas campestris

Abstract Several minimal media capable of inducing pathogenicity genes have been used to study plant–pathogen interactions. An in planta assay to study a closer interaction between the bacteria and the host was also developed and has been employed by our group. In order to determine whether growth m... Full description

Journal Title: FEMS microbiology letters 2017-12-01, Vol.364 (22)
Main Author: Maximiano, Mariana Rocha
Other Authors: Oliveira-Neto, Osmundo B , Franco, Octávio L , Mehta, Angela
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Alma/SFX Local Collection
Publisher: England: Oxford University Press
ID: ISSN: 1574-6968
Link: https://www.ncbi.nlm.nih.gov/pubmed/29040467
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: cdi_proquest_miscellaneous_1952525237
title: Validation of an in vitro system for studies of pathogenicity mechanisms in Xanthomonas campestris
format: Article
creator:
  • Maximiano, Mariana Rocha
  • Oliveira-Neto, Osmundo B
  • Franco, Octávio L
  • Mehta, Angela
subjects:
  • Bacterial Proteins - genetics
  • Bacterial Proteins - metabolism
  • Brassica - microbiology
  • Culture Media
  • Gene Expression Profiling
  • Gene Expression Regulation, Bacterial - genetics
  • Plant Diseases - microbiology
  • Plant Pathology
  • RNA, Bacterial - analysis
  • RNA, Bacterial - genetics
  • RNA, Bacterial - metabolism
  • RNA, Messenger - analysis
  • RNA, Messenger - genetics
  • RNA, Messenger - metabolism
  • Virulence - genetics
  • Xanthomonas campestris - genetics
  • Xanthomonas campestris - metabolism
  • Xanthomonas campestris - pathogenicity
ispartof: FEMS microbiology letters, 2017-12-01, Vol.364 (22)
description: Abstract Several minimal media capable of inducing pathogenicity genes have been used to study plant–pathogen interactions. An in planta assay to study a closer interaction between the bacteria and the host was also developed and has been employed by our group. In order to determine whether growth medium could be improved to better approximate in planta conditions beyond that offered by the defined minimal medium XVM1, we compared the expression of 20 Xanthomonas campestris pv. campestris (Xcc) genes by quantitative reverse transcription - polymerase chain reaction (qRT-PCR) under in vivo (bacteria recovered from the plant) and in vitro (rich medium NYG, minimal medium XVM1 and XVM1 + leaf extract) growth systems. The results showed a higher expression level of the genes in the in planta system when compared to growth in culture media. In planta growth is closest to a real interaction condition and captures the complexity of the plant cell environment; however, this system has some limitations. The main finding of our work is that the addition of plant extract to XVM1 medium results in a gene expression profile that better matches the in planta profile, when compared with the XVM1 medium alone, giving support to the use of plant extract to study pathogenicity mechanisms in Xanthomonas. The authors showed that addition of plant extract to a defined medium resulted in patterns of virulence-associated gene expression that more closely mimicked in-plant conditions than did defined medium alone.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 1574-6968
fulltext: fulltext
issn:
  • 1574-6968
  • 1574-6968
url: Link


@attributes
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
RANK2.4752676
LOCALfalse
PrimoNMBib
record
control
sourceidproquest_cross
recordidTN_cdi_proquest_miscellaneous_1952525237
sourceformatXML
sourcesystemPC
oup_id10.1093/femsle/fnx217
sourcerecordid1952525237
originalsourceidFETCH-LOGICAL-1330t-2cd7049b805d7ea8521256eb3eaf45b3522032bc9f2b903650626954fdd61ecd3
addsrcrecordideNqFkMtLxDAQh4Mo7rp69Co5eqmbR5Nuj7L4AsGLireStokbaZLaScX9723p-rjJHGZgvvkNfAidUnJBSc6XRjto9NL4T0azPTSnIksTmcvV_p95ho4A3gghKSPyEM1YTlKSymyOymfV2FpFGzwOBiuPrccfNnYBwxaidtiEDkPsa6thJFoVN-FVe1vZuMVOVxvlLTgY716UH5YueAW4Uq7VEDsLx-jAqAb0ya4v0NP11eP6Nrl_uLlbX94nlHMSE1bVGUnzckVEnWm1EowyIXXJtTKpKLlgjHBWVrlhZU64FEQymYvU1LWkuqr5Ap1PuW0X3vvhd-EsVLpplNehh4Lmgo3FswFNJrTqAkCnTdF21qluW1BSjFqLSWsxaR34s110Xzpd_9DfHn9_h779J-sL8J6Eag
sourcetypeAggregation Database
isCDItrue
recordtypearticle
pqid1952525237
display
typearticle
titleValidation of an in vitro system for studies of pathogenicity mechanisms in Xanthomonas campestris
sourceAlma/SFX Local Collection
creatorMaximiano, Mariana Rocha ; Oliveira-Neto, Osmundo B ; Franco, Octávio L ; Mehta, Angela
creatorcontribMaximiano, Mariana Rocha ; Oliveira-Neto, Osmundo B ; Franco, Octávio L ; Mehta, Angela
descriptionAbstract Several minimal media capable of inducing pathogenicity genes have been used to study plant–pathogen interactions. An in planta assay to study a closer interaction between the bacteria and the host was also developed and has been employed by our group. In order to determine whether growth medium could be improved to better approximate in planta conditions beyond that offered by the defined minimal medium XVM1, we compared the expression of 20 Xanthomonas campestris pv. campestris (Xcc) genes by quantitative reverse transcription - polymerase chain reaction (qRT-PCR) under in vivo (bacteria recovered from the plant) and in vitro (rich medium NYG, minimal medium XVM1 and XVM1 + leaf extract) growth systems. The results showed a higher expression level of the genes in the in planta system when compared to growth in culture media. In planta growth is closest to a real interaction condition and captures the complexity of the plant cell environment; however, this system has some limitations. The main finding of our work is that the addition of plant extract to XVM1 medium results in a gene expression profile that better matches the in planta profile, when compared with the XVM1 medium alone, giving support to the use of plant extract to study pathogenicity mechanisms in Xanthomonas. The authors showed that addition of plant extract to a defined medium resulted in patterns of virulence-associated gene expression that more closely mimicked in-plant conditions than did defined medium alone.
identifier
0ISSN: 1574-6968
1EISSN: 1574-6968
2DOI: 10.1093/femsle/fnx217
3PMID: 29040467
languageeng
publisherEngland: Oxford University Press
subjectBacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Brassica - microbiology ; Culture Media ; Gene Expression Profiling ; Gene Expression Regulation, Bacterial - genetics ; Plant Diseases - microbiology ; Plant Pathology ; RNA, Bacterial - analysis ; RNA, Bacterial - genetics ; RNA, Bacterial - metabolism ; RNA, Messenger - analysis ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Virulence - genetics ; Xanthomonas campestris - genetics ; Xanthomonas campestris - metabolism ; Xanthomonas campestris - pathogenicity
ispartofFEMS microbiology letters, 2017-12-01, Vol.364 (22)
rights
0FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com 2017
1FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
lds50peer_reviewed
oafree_for_read
citesFETCH-LOGICAL-1330t-2cd7049b805d7ea8521256eb3eaf45b3522032bc9f2b903650626954fdd61ecd3
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
thumbnail$$Usyndetics_thumb_exl
backlink$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29040467$$D View this record in MEDLINE/PubMed
search
creatorcontrib
0Maximiano, Mariana Rocha
1Oliveira-Neto, Osmundo B
2Franco, Octávio L
3Mehta, Angela
title
0Validation of an in vitro system for studies of pathogenicity mechanisms in Xanthomonas campestris
1FEMS microbiology letters
addtitleFEMS Microbiol Lett
descriptionAbstract Several minimal media capable of inducing pathogenicity genes have been used to study plant–pathogen interactions. An in planta assay to study a closer interaction between the bacteria and the host was also developed and has been employed by our group. In order to determine whether growth medium could be improved to better approximate in planta conditions beyond that offered by the defined minimal medium XVM1, we compared the expression of 20 Xanthomonas campestris pv. campestris (Xcc) genes by quantitative reverse transcription - polymerase chain reaction (qRT-PCR) under in vivo (bacteria recovered from the plant) and in vitro (rich medium NYG, minimal medium XVM1 and XVM1 + leaf extract) growth systems. The results showed a higher expression level of the genes in the in planta system when compared to growth in culture media. In planta growth is closest to a real interaction condition and captures the complexity of the plant cell environment; however, this system has some limitations. The main finding of our work is that the addition of plant extract to XVM1 medium results in a gene expression profile that better matches the in planta profile, when compared with the XVM1 medium alone, giving support to the use of plant extract to study pathogenicity mechanisms in Xanthomonas. The authors showed that addition of plant extract to a defined medium resulted in patterns of virulence-associated gene expression that more closely mimicked in-plant conditions than did defined medium alone.
subject
0Bacterial Proteins - genetics
1Bacterial Proteins - metabolism
2Brassica - microbiology
3Culture Media
4Gene Expression Profiling
5Gene Expression Regulation, Bacterial - genetics
6Plant Diseases - microbiology
7Plant Pathology
8RNA, Bacterial - analysis
9RNA, Bacterial - genetics
10RNA, Bacterial - metabolism
11RNA, Messenger - analysis
12RNA, Messenger - genetics
13RNA, Messenger - metabolism
14Virulence - genetics
15Xanthomonas campestris - genetics
16Xanthomonas campestris - metabolism
17Xanthomonas campestris - pathogenicity
issn
01574-6968
11574-6968
fulltexttrue
rsrctypearticle
creationdate2017
recordtypearticle
recordideNqFkMtLxDAQh4Mo7rp69Co5eqmbR5Nuj7L4AsGLireStokbaZLaScX9723p-rjJHGZgvvkNfAidUnJBSc6XRjto9NL4T0azPTSnIksTmcvV_p95ho4A3gghKSPyEM1YTlKSymyOymfV2FpFGzwOBiuPrccfNnYBwxaidtiEDkPsa6thJFoVN-FVe1vZuMVOVxvlLTgY716UH5YueAW4Uq7VEDsLx-jAqAb0ya4v0NP11eP6Nrl_uLlbX94nlHMSE1bVGUnzckVEnWm1EowyIXXJtTKpKLlgjHBWVrlhZU64FEQymYvU1LWkuqr5Ap1PuW0X3vvhd-EsVLpplNehh4Lmgo3FswFNJrTqAkCnTdF21qluW1BSjFqLSWsxaR34s110Xzpd_9DfHn9_h779J-sL8J6Eag
startdate20171201
enddate20171201
creator
0Maximiano, Mariana Rocha
1Oliveira-Neto, Osmundo B
2Franco, Octávio L
3Mehta, Angela
generalOxford University Press
scope
0CGR
1CUY
2CVF
3ECM
4EIF
5NPM
6AAYXX
7CITATION
87X8
sort
creationdate20171201
titleValidation of an in vitro system for studies of pathogenicity mechanisms in Xanthomonas campestris
authorMaximiano, Mariana Rocha ; Oliveira-Neto, Osmundo B ; Franco, Octávio L ; Mehta, Angela
facets
frbrtype5
frbrgroupidcdi_FETCH-LOGICAL-1330t-2cd7049b805d7ea8521256eb3eaf45b3522032bc9f2b903650626954fdd61ecd3
rsrctypearticles
prefilterarticles
languageeng
creationdate2017
topic
0Bacterial Proteins - genetics
1Bacterial Proteins - metabolism
2Brassica - microbiology
3Culture Media
4Gene Expression Profiling
5Gene Expression Regulation, Bacterial - genetics
6Plant Diseases - microbiology
7Plant Pathology
8RNA, Bacterial - analysis
9RNA, Bacterial - genetics
10RNA, Bacterial - metabolism
11RNA, Messenger - analysis
12RNA, Messenger - genetics
13RNA, Messenger - metabolism
14Virulence - genetics
15Xanthomonas campestris - genetics
16Xanthomonas campestris - metabolism
17Xanthomonas campestris - pathogenicity
toplevel
0peer_reviewed
1online_resources
creatorcontrib
0Maximiano, Mariana Rocha
1Oliveira-Neto, Osmundo B
2Franco, Octávio L
3Mehta, Angela
collection
0Medline
1MEDLINE
2MEDLINE (Ovid)
3MEDLINE
4MEDLINE
5PubMed
6CrossRef
7MEDLINE - Academic
jtitleFEMS microbiology letters
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
au
0Maximiano, Mariana Rocha
1Oliveira-Neto, Osmundo B
2Franco, Octávio L
3Mehta, Angela
formatjournal
genrearticle
ristypeJOUR
atitleValidation of an in vitro system for studies of pathogenicity mechanisms in Xanthomonas campestris
jtitleFEMS microbiology letters
addtitleFEMS Microbiol Lett
date2017-12-01
risdate2017
volume364
issue22
issn1574-6968
eissn1574-6968
abstractAbstract Several minimal media capable of inducing pathogenicity genes have been used to study plant–pathogen interactions. An in planta assay to study a closer interaction between the bacteria and the host was also developed and has been employed by our group. In order to determine whether growth medium could be improved to better approximate in planta conditions beyond that offered by the defined minimal medium XVM1, we compared the expression of 20 Xanthomonas campestris pv. campestris (Xcc) genes by quantitative reverse transcription - polymerase chain reaction (qRT-PCR) under in vivo (bacteria recovered from the plant) and in vitro (rich medium NYG, minimal medium XVM1 and XVM1 + leaf extract) growth systems. The results showed a higher expression level of the genes in the in planta system when compared to growth in culture media. In planta growth is closest to a real interaction condition and captures the complexity of the plant cell environment; however, this system has some limitations. The main finding of our work is that the addition of plant extract to XVM1 medium results in a gene expression profile that better matches the in planta profile, when compared with the XVM1 medium alone, giving support to the use of plant extract to study pathogenicity mechanisms in Xanthomonas. The authors showed that addition of plant extract to a defined medium resulted in patterns of virulence-associated gene expression that more closely mimicked in-plant conditions than did defined medium alone.
copEngland
pubOxford University Press
pmid29040467
doi10.1093/femsle/fnx217
oafree_for_read