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Transformation From a Conventional Clinical Microbiology Laboratory to Full Automation

Abstract Background To validate the performance, reproducibility, and reliability of BD automated instruments in order to establish a fully automated clinical microbiology laboratory. Methods We used control strains and clinical samples to assess the accuracy, reproducibility, and reliability of the... Full description

Journal Title: Laboratory medicine 2017-12-22, Vol.49 (1), p.e1-e8
Main Author: Moreno-Camacho, José L
Other Authors: Calva-Espinosa, Diana Y , Leal-Leyva, Yoseli Y , Elizalde-Olivas, Dolores C , Campos-Romero, Abraham , Alcántar-Fernández, Jonathan
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: US: Oxford University Press
ID: ISSN: 0007-5027
Link: https://www.ncbi.nlm.nih.gov/pubmed/29253199
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recordid: cdi_proquest_miscellaneous_1978718140
title: Transformation From a Conventional Clinical Microbiology Laboratory to Full Automation
format: Article
creator:
  • Moreno-Camacho, José L
  • Calva-Espinosa, Diana Y
  • Leal-Leyva, Yoseli Y
  • Elizalde-Olivas, Dolores C
  • Campos-Romero, Abraham
  • Alcántar-Fernández, Jonathan
subjects:
  • Antibiotics
  • Automation
  • Automation, Laboratory - instrumentation
  • Automation, Laboratory - methods
  • Automation, Laboratory - standards
  • Bacterial Load
  • Bacteriological Techniques - instrumentation
  • Bacteriological Techniques - methods
  • Bacteriological Techniques - standards
  • Humans
  • Laboratories
  • Microbial Sensitivity Tests
  • Molecular Typing
  • Reproducibility of Results
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
ispartof: Laboratory medicine, 2017-12-22, Vol.49 (1), p.e1-e8
description: Abstract Background To validate the performance, reproducibility, and reliability of BD automated instruments in order to establish a fully automated clinical microbiology laboratory. Methods We used control strains and clinical samples to assess the accuracy, reproducibility, and reliability of the BD Kiestra WCA, the BD Phoenix, and BD Bruker MALDI-Biotyper instruments and compared them to previously established conventional methods. The following processes were evaluated: sample inoculation and spreading, colony counts, sorting of cultures, antibiotic susceptibility test, and microbial identification. Results The BD Kiestra recovered single colonies in less time than conventional methods (e.g. E. coli, 7h vs 10h, respectively) and agreement between both methodologies was excellent for colony counts (κ=0.824) and sorting cultures (κ=0.821). Antibiotic susceptibility tests performed with BD Phoenix and disk diffusion demonstrated 96.3% agreement with both methods. Finally, we compared microbial identification in BD Phoenix and Bruker MALDI-Biotyper and observed perfect agreement (κ=1) and identification at a species level for control strains. Together these instruments allow us to process clinical urine samples in 36h (effective time). Conclusion The BD automated technologies have improved performance compared with conventional methods, and are suitable for its implementation in very busy microbiology laboratories.
language: eng
source:
identifier: ISSN: 0007-5027
fulltext: no_fulltext
issn:
  • 0007-5027
  • 1943-7730
url: Link


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titleTransformation From a Conventional Clinical Microbiology Laboratory to Full Automation
creatorMoreno-Camacho, José L ; Calva-Espinosa, Diana Y ; Leal-Leyva, Yoseli Y ; Elizalde-Olivas, Dolores C ; Campos-Romero, Abraham ; Alcántar-Fernández, Jonathan
creatorcontribMoreno-Camacho, José L ; Calva-Espinosa, Diana Y ; Leal-Leyva, Yoseli Y ; Elizalde-Olivas, Dolores C ; Campos-Romero, Abraham ; Alcántar-Fernández, Jonathan
descriptionAbstract Background To validate the performance, reproducibility, and reliability of BD automated instruments in order to establish a fully automated clinical microbiology laboratory. Methods We used control strains and clinical samples to assess the accuracy, reproducibility, and reliability of the BD Kiestra WCA, the BD Phoenix, and BD Bruker MALDI-Biotyper instruments and compared them to previously established conventional methods. The following processes were evaluated: sample inoculation and spreading, colony counts, sorting of cultures, antibiotic susceptibility test, and microbial identification. Results The BD Kiestra recovered single colonies in less time than conventional methods (e.g. E. coli, 7h vs 10h, respectively) and agreement between both methodologies was excellent for colony counts (κ=0.824) and sorting cultures (κ=0.821). Antibiotic susceptibility tests performed with BD Phoenix and disk diffusion demonstrated 96.3% agreement with both methods. Finally, we compared microbial identification in BD Phoenix and Bruker MALDI-Biotyper and observed perfect agreement (κ=1) and identification at a species level for control strains. Together these instruments allow us to process clinical urine samples in 36h (effective time). Conclusion The BD automated technologies have improved performance compared with conventional methods, and are suitable for its implementation in very busy microbiology laboratories.
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subjectAntibiotics ; Automation ; Automation, Laboratory - instrumentation ; Automation, Laboratory - methods ; Automation, Laboratory - standards ; Bacterial Load ; Bacteriological Techniques - instrumentation ; Bacteriological Techniques - methods ; Bacteriological Techniques - standards ; Humans ; Laboratories ; Microbial Sensitivity Tests ; Molecular Typing ; Reproducibility of Results ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
ispartofLaboratory medicine, 2017-12-22, Vol.49 (1), p.e1-e8
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0American Society for Clinical Pathology 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com 2017
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descriptionAbstract Background To validate the performance, reproducibility, and reliability of BD automated instruments in order to establish a fully automated clinical microbiology laboratory. Methods We used control strains and clinical samples to assess the accuracy, reproducibility, and reliability of the BD Kiestra WCA, the BD Phoenix, and BD Bruker MALDI-Biotyper instruments and compared them to previously established conventional methods. The following processes were evaluated: sample inoculation and spreading, colony counts, sorting of cultures, antibiotic susceptibility test, and microbial identification. Results The BD Kiestra recovered single colonies in less time than conventional methods (e.g. E. coli, 7h vs 10h, respectively) and agreement between both methodologies was excellent for colony counts (κ=0.824) and sorting cultures (κ=0.821). Antibiotic susceptibility tests performed with BD Phoenix and disk diffusion demonstrated 96.3% agreement with both methods. Finally, we compared microbial identification in BD Phoenix and Bruker MALDI-Biotyper and observed perfect agreement (κ=1) and identification at a species level for control strains. Together these instruments allow us to process clinical urine samples in 36h (effective time). Conclusion The BD automated technologies have improved performance compared with conventional methods, and are suitable for its implementation in very busy microbiology laboratories.
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0Antibiotics
1Automation
2Automation, Laboratory - instrumentation
3Automation, Laboratory - methods
4Automation, Laboratory - standards
5Bacterial Load
6Bacteriological Techniques - instrumentation
7Bacteriological Techniques - methods
8Bacteriological Techniques - standards
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10Laboratories
11Microbial Sensitivity Tests
12Molecular Typing
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abstractAbstract Background To validate the performance, reproducibility, and reliability of BD automated instruments in order to establish a fully automated clinical microbiology laboratory. Methods We used control strains and clinical samples to assess the accuracy, reproducibility, and reliability of the BD Kiestra WCA, the BD Phoenix, and BD Bruker MALDI-Biotyper instruments and compared them to previously established conventional methods. The following processes were evaluated: sample inoculation and spreading, colony counts, sorting of cultures, antibiotic susceptibility test, and microbial identification. Results The BD Kiestra recovered single colonies in less time than conventional methods (e.g. E. coli, 7h vs 10h, respectively) and agreement between both methodologies was excellent for colony counts (κ=0.824) and sorting cultures (κ=0.821). Antibiotic susceptibility tests performed with BD Phoenix and disk diffusion demonstrated 96.3% agreement with both methods. Finally, we compared microbial identification in BD Phoenix and Bruker MALDI-Biotyper and observed perfect agreement (κ=1) and identification at a species level for control strains. Together these instruments allow us to process clinical urine samples in 36h (effective time). Conclusion The BD automated technologies have improved performance compared with conventional methods, and are suitable for its implementation in very busy microbiology laboratories.
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