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Precise genome modification in the crop species Zea mays using zinc-finger nucleases

Agricultural biotechnology is limited by the inefficiencies of conventional random mutagenesis and transgenesis. Because targeted genome modification in plants has been intractable, plant trait engineering remains a laborious, time-consuming and unpredictable undertaking. Here we report a broadly ap... Full description

Journal Title: Nature 2009, Vol.459 (7245), p.437-441
Main Author: SHUKLA, Vipula K
Other Authors: DOYON, Yannick , CHOI, Vivian M , ROCK, Jeremy M , WU, Ying-Ying , KATIBAH, George E , GAO ZHIFANG , MCCASKILL, David , SIMPSON, Matthew A , BLAKESLEE, Beth , GREENWALT, Scott A , BUTLER, Holly J , MILLER, Jeffrey C , HINKLEY, Sarah J , LEI ZHANG , REBAR, Edward J , GREGORY, Philip D , URNOV, Fyodor D , DEKELVER, Russell C , MOEHLE, Erica A , WORDEN, Sarah E , MITCHELL, Jon C , ARNOLD, Nicole L , GOPALAN, Sunita , XIANGDONG MENG
Format: Electronic Article Electronic Article
Language: English
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Publisher: London: Nature Publishing Group
ID: ISSN: 0028-0836
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recordid: cdi_proquest_miscellaneous_20623636
title: Precise genome modification in the crop species Zea mays using zinc-finger nucleases
format: Article
creator:
  • SHUKLA, Vipula K
  • DOYON, Yannick
  • CHOI, Vivian M
  • ROCK, Jeremy M
  • WU, Ying-Ying
  • KATIBAH, George E
  • GAO ZHIFANG
  • MCCASKILL, David
  • SIMPSON, Matthew A
  • BLAKESLEE, Beth
  • GREENWALT, Scott A
  • BUTLER, Holly J
  • MILLER, Jeffrey C
  • HINKLEY, Sarah J
  • LEI ZHANG
  • REBAR, Edward J
  • GREGORY, Philip D
  • URNOV, Fyodor D
  • DEKELVER, Russell C
  • MOEHLE, Erica A
  • WORDEN, Sarah E
  • MITCHELL, Jon C
  • ARNOLD, Nicole L
  • GOPALAN, Sunita
  • XIANGDONG MENG
subjects:
  • Agricultural biotechnology
  • Biological and medical sciences
  • Biotechnology - methods
  • Corn
  • Deoxyribonucleases - chemistry
  • Deoxyribonucleases - genetics
  • Deoxyribonucleases - metabolism
  • Food, Genetically Modified
  • Gene Targeting - methods
  • General aspects
  • Genes, Plant - genetics
  • Genetic aspects
  • Genome, Plant - genetics
  • Genomics
  • Herbicide Resistance - genetics
  • Herbicides - pharmacology
  • Heredity
  • Infectious diseases
  • Inositol Phosphates - metabolism
  • Medical sciences
  • Mutagenesis, Site-Directed - methods
  • Mycoses
  • Plants, Genetically Modified
  • Recombination, Genetic - genetics
  • Reproducibility of Results
  • Usage
  • Zea mays
  • Zea mays - genetics
  • Zinc finger proteins
  • Zinc Fingers
ispartof: Nature, 2009, Vol.459 (7245), p.437-441
description: Agricultural biotechnology is limited by the inefficiencies of conventional random mutagenesis and transgenesis. Because targeted genome modification in plants has been intractable, plant trait engineering remains a laborious, time-consuming and unpredictable undertaking. Here we report a broadly applicable, versatile solution to this problem: the use of designed zinc-finger nucleases (ZFNs) that induce a double-stranded break at their target locus. We describe the use of ZFNs to modify endogenous loci in plants of the crop species Zea mays. We show that simultaneous expression of ZFNs and delivery of a simple heterologous donor molecule leads to precise targeted addition of an herbicide-tolerance gene at the intended locus in a significant number of isolated events. ZFN-modified maize plants faithfully transmit these genetic changes to the next generation. Insertional disruption of one target locus, IPK1, results in both herbicide tolerance and the expected alteration of the inositol phosphate profile in developing seeds. ZFNs can be used in any plant species amenable to DNA delivery; our results therefore establish a new strategy for plant genetic manipulation in basic science and agricultural applications.
language: eng
source:
identifier: ISSN: 0028-0836
fulltext: no_fulltext
issn:
  • 0028-0836
  • 1476-4687
  • 1476-4679
url: Link


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titlePrecise genome modification in the crop species Zea mays using zinc-finger nucleases
creatorSHUKLA, Vipula K ; DOYON, Yannick ; CHOI, Vivian M ; ROCK, Jeremy M ; WU, Ying-Ying ; KATIBAH, George E ; GAO ZHIFANG ; MCCASKILL, David ; SIMPSON, Matthew A ; BLAKESLEE, Beth ; GREENWALT, Scott A ; BUTLER, Holly J ; MILLER, Jeffrey C ; HINKLEY, Sarah J ; LEI ZHANG ; REBAR, Edward J ; GREGORY, Philip D ; URNOV, Fyodor D ; DEKELVER, Russell C ; MOEHLE, Erica A ; WORDEN, Sarah E ; MITCHELL, Jon C ; ARNOLD, Nicole L ; GOPALAN, Sunita ; XIANGDONG MENG
creatorcontribSHUKLA, Vipula K ; DOYON, Yannick ; CHOI, Vivian M ; ROCK, Jeremy M ; WU, Ying-Ying ; KATIBAH, George E ; GAO ZHIFANG ; MCCASKILL, David ; SIMPSON, Matthew A ; BLAKESLEE, Beth ; GREENWALT, Scott A ; BUTLER, Holly J ; MILLER, Jeffrey C ; HINKLEY, Sarah J ; LEI ZHANG ; REBAR, Edward J ; GREGORY, Philip D ; URNOV, Fyodor D ; DEKELVER, Russell C ; MOEHLE, Erica A ; WORDEN, Sarah E ; MITCHELL, Jon C ; ARNOLD, Nicole L ; GOPALAN, Sunita ; XIANGDONG MENG
descriptionAgricultural biotechnology is limited by the inefficiencies of conventional random mutagenesis and transgenesis. Because targeted genome modification in plants has been intractable, plant trait engineering remains a laborious, time-consuming and unpredictable undertaking. Here we report a broadly applicable, versatile solution to this problem: the use of designed zinc-finger nucleases (ZFNs) that induce a double-stranded break at their target locus. We describe the use of ZFNs to modify endogenous loci in plants of the crop species Zea mays. We show that simultaneous expression of ZFNs and delivery of a simple heterologous donor molecule leads to precise targeted addition of an herbicide-tolerance gene at the intended locus in a significant number of isolated events. ZFN-modified maize plants faithfully transmit these genetic changes to the next generation. Insertional disruption of one target locus, IPK1, results in both herbicide tolerance and the expected alteration of the inositol phosphate profile in developing seeds. ZFNs can be used in any plant species amenable to DNA delivery; our results therefore establish a new strategy for plant genetic manipulation in basic science and agricultural applications.
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subjectAgricultural biotechnology ; Biological and medical sciences ; Biotechnology - methods ; Corn ; Deoxyribonucleases - chemistry ; Deoxyribonucleases - genetics ; Deoxyribonucleases - metabolism ; Food, Genetically Modified ; Gene Targeting - methods ; General aspects ; Genes, Plant - genetics ; Genetic aspects ; Genome, Plant - genetics ; Genomics ; Herbicide Resistance - genetics ; Herbicides - pharmacology ; Heredity ; Infectious diseases ; Inositol Phosphates - metabolism ; Medical sciences ; Mutagenesis, Site-Directed - methods ; Mycoses ; Plants, Genetically Modified ; Recombination, Genetic - genetics ; Reproducibility of Results ; Usage ; Zea mays ; Zea mays - genetics ; Zinc finger proteins ; Zinc Fingers
ispartofNature, 2009, Vol.459 (7245), p.437-441
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2CHOI, Vivian M
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16GREGORY, Philip D
17URNOV, Fyodor D
18DEKELVER, Russell C
19MOEHLE, Erica A
20WORDEN, Sarah E
21MITCHELL, Jon C
22ARNOLD, Nicole L
23GOPALAN, Sunita
24XIANGDONG MENG
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descriptionAgricultural biotechnology is limited by the inefficiencies of conventional random mutagenesis and transgenesis. Because targeted genome modification in plants has been intractable, plant trait engineering remains a laborious, time-consuming and unpredictable undertaking. Here we report a broadly applicable, versatile solution to this problem: the use of designed zinc-finger nucleases (ZFNs) that induce a double-stranded break at their target locus. We describe the use of ZFNs to modify endogenous loci in plants of the crop species Zea mays. We show that simultaneous expression of ZFNs and delivery of a simple heterologous donor molecule leads to precise targeted addition of an herbicide-tolerance gene at the intended locus in a significant number of isolated events. ZFN-modified maize plants faithfully transmit these genetic changes to the next generation. Insertional disruption of one target locus, IPK1, results in both herbicide tolerance and the expected alteration of the inositol phosphate profile in developing seeds. ZFNs can be used in any plant species amenable to DNA delivery; our results therefore establish a new strategy for plant genetic manipulation in basic science and agricultural applications.
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0Agricultural biotechnology
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4Deoxyribonucleases - chemistry
5Deoxyribonucleases - genetics
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7Food, Genetically Modified
8Gene Targeting - methods
9General aspects
10Genes, Plant - genetics
11Genetic aspects
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14Herbicide Resistance - genetics
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titlePrecise genome modification in the crop species Zea mays using zinc-finger nucleases
authorSHUKLA, Vipula K ; DOYON, Yannick ; CHOI, Vivian M ; ROCK, Jeremy M ; WU, Ying-Ying ; KATIBAH, George E ; GAO ZHIFANG ; MCCASKILL, David ; SIMPSON, Matthew A ; BLAKESLEE, Beth ; GREENWALT, Scott A ; BUTLER, Holly J ; MILLER, Jeffrey C ; HINKLEY, Sarah J ; LEI ZHANG ; REBAR, Edward J ; GREGORY, Philip D ; URNOV, Fyodor D ; DEKELVER, Russell C ; MOEHLE, Erica A ; WORDEN, Sarah E ; MITCHELL, Jon C ; ARNOLD, Nicole L ; GOPALAN, Sunita ; XIANGDONG MENG
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1Biological and medical sciences
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4Deoxyribonucleases - chemistry
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6Deoxyribonucleases - metabolism
7Food, Genetically Modified
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27Zea mays - genetics
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abstractAgricultural biotechnology is limited by the inefficiencies of conventional random mutagenesis and transgenesis. Because targeted genome modification in plants has been intractable, plant trait engineering remains a laborious, time-consuming and unpredictable undertaking. Here we report a broadly applicable, versatile solution to this problem: the use of designed zinc-finger nucleases (ZFNs) that induce a double-stranded break at their target locus. We describe the use of ZFNs to modify endogenous loci in plants of the crop species Zea mays. We show that simultaneous expression of ZFNs and delivery of a simple heterologous donor molecule leads to precise targeted addition of an herbicide-tolerance gene at the intended locus in a significant number of isolated events. ZFN-modified maize plants faithfully transmit these genetic changes to the next generation. Insertional disruption of one target locus, IPK1, results in both herbicide tolerance and the expected alteration of the inositol phosphate profile in developing seeds. ZFNs can be used in any plant species amenable to DNA delivery; our results therefore establish a new strategy for plant genetic manipulation in basic science and agricultural applications.
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